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Offlineipappnasei
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Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
at what point should one get a laminar flowhood or gloveboxes?
    #21778244 - 06/08/15 10:57 AM (8 years, 7 months ago)

And does anyone here build and sell those home made flow hoods? I never used either


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InvisibleMyconin
Mushroom Ninja
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Registered: 05/11/15
Posts: 308
Loc: The shadows... Flag
Re: at what point should one get a laminar flowhood or gloveboxes? [Re: ipappnasei]
    #21778262 - 06/08/15 11:01 AM (8 years, 7 months ago)

There are tons of pictorials on this site on how to build either one. I've never built a flow hood personally, I prefer to use a SAB. You can build one of them for as little as $10-$15 with materials found at any walmart or similar store.


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Offlineipappnasei
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Registered: 10/13/13
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Last seen: 6 years, 4 months
Re: at what point should one get a laminar flowhood or gloveboxes? [Re: Myconin]
    #21778301 - 06/08/15 11:14 AM (8 years, 7 months ago)

Quote:

Myconin said:
There are tons of pictorials on this site on how to build either one. I've never built a flow hood personally, I prefer to use a SAB. You can build one of them for as little as $10-$15 with materials found at any walmart or similar store.





Yea after looking at how tos i figured a sab is more doable for me. Im wondering right now how do you get the jars into the box safely? I mean they gonna have air contact and the inside of the box too?


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InvisibleMyconin
Mushroom Ninja
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Registered: 05/11/15
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: ipappnasei]
    #21778333 - 06/08/15 11:21 AM (8 years, 7 months ago)

Right. You put everything you'll need in the box (jars, syringe, etc) then close it up. I always keep a spray bottle of lysol and lysol wipes in the box, but soapy water will work. Spray the inside of the box, while closed, with lysol or soapy water and let it sit for about an hour or so to let everything settle. Then, wipe down the interior with lysol or iso alcohol, and go to work :grin:

This process may change depending on your design. Some people have open holes on their SAB which they plug up when not in use, while others have permanent gloves attached to the holes. You'll have to engineer something that works for your project with what you have available.

Hope this helped :thumbup:


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Offlineipappnasei
Male


Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
Re: at what point should one get a laminar flowhood or gloveboxes? [Re: Myconin]
    #21778405 - 06/08/15 11:38 AM (8 years, 7 months ago)

Quote:

Myconin said:
Right. You put everything you'll need in the box (jars, syringe, etc) then close it up. I always keep a spray bottle of lysol and lysol wipes in the box, but soapy water will work. Spray the inside of the box, while closed, with lysol or soapy water and let it sit for about an hour or so to let everything settle. Then, wipe down the interior with lysol or iso alcohol, and go to work :grin:

This process may change depending on your design. Some people have open holes on their SAB which they plug up when not in use, while others have permanent gloves attached to the holes. You'll have to engineer something that works for your project with what you have available.

Hope this helped :thumbup:






Yes this helped a lot. Just one more ting. From personal exprience do you think holes or permanent gloves is the way to go?


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Offlinethoraxx
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Registered: 12/27/13
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: Myconin]
    #21778433 - 06/08/15 11:45 AM (8 years, 7 months ago)

I cant see how a flow hood is nessecary for anyone but large scale growers
As a hobby grower it makes no difference if you have 10-20% contamination vs <5% or whatever

I just started a new grow, reviving spores from my last grow about a year ago
Now agar work is propably the most contamination prone, but i just do everything in my kitchen without much more than an alcohol soaked rag and theres no problem really
A couple of plates contam after inocc (never after pouring), but spore prints arent sterile in the first place and thats the reason for working with agar anyway
I can toss those, as long as one plate survives with a healthy sector thats all i need, from there contaminations are minimal and i can finish working toward an isolated culture

I guess it depends on the location/condition of your workspace, but you should try out how much contamination you actually get with different approaches
For me its much more convenient to just work out in the open, without long prep work, and just make enough petris and jars so i can toss out a few contams here and there vs making a big fuss with my SAB (which still doesnt rule out contams) and ultimately wind up with less because it kills my motivation
In the time it takes me to get the box out of storage, wipe and spray everything, put the stuff in there and try to accomodate an open flame around alcohol soaked material, i can just put everything up on a clean surface, maybe wear a mask and gloves and be done with twice the work

Once you start to inocc with healthy agar, contam risk on your jars goes down significantly anyway


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Offlineipappnasei
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: thoraxx]
    #21778464 - 06/08/15 11:52 AM (8 years, 7 months ago)

Thx for replies. Is there someone with a room fully devoted to mushroom growing and nothing else? Id like to see that.


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Invisiblegrainbrain
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Registered: 05/11/11
Posts: 2,626
Re: at what point should one get a laminar flowhood or gloveboxes? [Re: ipappnasei]
    #21778551 - 06/08/15 12:20 PM (8 years, 7 months ago)

I started off working in open air, then moved to a SAB when I got into agar.  Purchased a flowhood a year later & don't regret it one bit.  It just depends on what your needs are and how serious you are about the hobby. 



--------------------
Stop cold shocking your mycelium!
Hot Spawn - Get It On



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OfflineGreenRabbit
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: grainbrain]
    #21778567 - 06/08/15 12:25 PM (8 years, 7 months ago)

Gloveboxes move air around more than a SAB. You flame sterilize outside the SAB anyway. Pretty sure people don't even use gloveboxes anymore.


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Offlineipappnasei
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Registered: 10/13/13
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: GreenRabbit]
    #21778638 - 06/08/15 12:47 PM (8 years, 7 months ago)

Quote:

GreenRabbit said:
Gloveboxes move air around more than a SAB. You flame sterilize outside the SAB anyway. Pretty sure people don't even use gloveboxes anymore.





I fail to see how. Wouldnt you push in air without gloves ? Pls explain if you feel like it


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Offlineipappnasei
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Registered: 10/13/13
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: grainbrain]
    #21778649 - 06/08/15 12:49 PM (8 years, 7 months ago)

Quote:

grainbrain said:
I started off working in open air, then moved to a SAB when I got into agar.  Purchased a flowhood a year later & don't regret it one bit.  It just depends on what your needs are and how serious you are about the hobby. 







Would you mind providing a purchase link for future use?


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Invisiblegrainbrain
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: ipappnasei]
    #21778663 - 06/08/15 12:53 PM (8 years, 7 months ago)



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InvisibleGrey
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: ipappnasei]
    #21778692 - 06/08/15 12:58 PM (8 years, 7 months ago)

When using a SAB, you need have all fans and a.c. in the house and rooms off. The more still the air in the house, the more still the air is in your SAB. When you put your hands into the SAB, air is allowed to exit the arm holes rather than be pushed around inside like a glove box would.

Even if you made a glove box air tight, the stretching of the gloves would stir up the air inside the box making it useless.


--------------------


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Offlinethoraxx
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: grainbrain]
    #21778698 - 06/08/15 01:01 PM (8 years, 7 months ago)

Thats a decent price
Im sure if you can spare the money its worth it for convenience of a sterile atmosphere than you can just turn on with a button, but just for growing psilos idk


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Offlineipappnasei
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: Grey]
    #21778699 - 06/08/15 01:02 PM (8 years, 7 months ago)

Oh i see. Thx


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Offlineipappnasei
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: grainbrain]
    #21778713 - 06/08/15 01:06 PM (8 years, 7 months ago)

Quote:

grainbrain said:
Fungi Perfecti Series I  :thumbup: :thumbup:




Thats nice. Did you try if it works properly? Ive searched the forums here for selfmade ones and on every thread rogerrabbit was like " thats not a laminaor flow hood because..." :p


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InvisibleMad Season
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: ipappnasei]
    #21778742 - 06/08/15 01:12 PM (8 years, 7 months ago)

That and we flame sterilize our needles and tools. You can't have a flame in the glove box. Especially if it was doused in alcohol. You'd see a huge explosion in your face like some have lol. So you pull your arms out, flame the tool, then put it back in. It's all good


--------------------
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Edited by Mad Season (06/08/15 01:13 PM)


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Invisiblegrainbrain
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: ipappnasei]
    #21778750 - 06/08/15 01:14 PM (8 years, 7 months ago)

Quote:

ipappnasei said:
Quote:

grainbrain said:
Fungi Perfecti Series I  :thumbup: :thumbup:




Thats nice. Did you try if it works properly? Ive searched the forums here for selfmade ones and on every thread rogerrabbit was like " thats not a laminaor flow hood because..." :p





It works properly.  :thumbup:


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Offlineipappnasei
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: Mad Season]
    #21778782 - 06/08/15 01:21 PM (8 years, 7 months ago)

Quote:

Mad Season said:
That and we flame sterilize our needles and tools. You can't have a flame in the glove box. Especially if it was doused in alcohol. You'd see a huge explosion in your face like some have lol. So you pull your arms out, flame the tool, then put it back in. It's all good





Haha are there really people that did that?


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Offlineipappnasei
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Registered: 10/13/13
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: grainbrain]
    #21778787 - 06/08/15 01:22 PM (8 years, 7 months ago)

Quote:

grainbrain said:
Quote:

ipappnasei said:
Quote:

grainbrain said:
Fungi Perfecti Series I  :thumbup: :thumbup:




Thats nice. Did you try if it works properly? Ive searched the forums here for selfmade ones and on every thread rogerrabbit was like " thats not a laminaor flow hood because..." :p





It works properly.  :thumbup:




Amazing. Is this like your hobby or is it a business?


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