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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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at what point should one get a laminar flowhood or gloveboxes?
#21778244 - 06/08/15 10:57 AM (8 years, 7 months ago) |
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And does anyone here build and sell those home made flow hoods? I never used either
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: ipappnasei]
#21778262 - 06/08/15 11:01 AM (8 years, 7 months ago) |
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There are tons of pictorials on this site on how to build either one. I've never built a flow hood personally, I prefer to use a SAB. You can build one of them for as little as $10-$15 with materials found at any walmart or similar store.
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: Myconin]
#21778301 - 06/08/15 11:14 AM (8 years, 7 months ago) |
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Quote:
Myconin said: There are tons of pictorials on this site on how to build either one. I've never built a flow hood personally, I prefer to use a SAB. You can build one of them for as little as $10-$15 with materials found at any walmart or similar store.
Yea after looking at how tos i figured a sab is more doable for me. Im wondering right now how do you get the jars into the box safely? I mean they gonna have air contact and the inside of the box too?
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: ipappnasei]
#21778333 - 06/08/15 11:21 AM (8 years, 7 months ago) |
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Right. You put everything you'll need in the box (jars, syringe, etc) then close it up. I always keep a spray bottle of lysol and lysol wipes in the box, but soapy water will work. Spray the inside of the box, while closed, with lysol or soapy water and let it sit for about an hour or so to let everything settle. Then, wipe down the interior with lysol or iso alcohol, and go to work 
This process may change depending on your design. Some people have open holes on their SAB which they plug up when not in use, while others have permanent gloves attached to the holes. You'll have to engineer something that works for your project with what you have available.
Hope this helped
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: Myconin]
#21778405 - 06/08/15 11:38 AM (8 years, 7 months ago) |
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Quote:
Myconin said: Right. You put everything you'll need in the box (jars, syringe, etc) then close it up. I always keep a spray bottle of lysol and lysol wipes in the box, but soapy water will work. Spray the inside of the box, while closed, with lysol or soapy water and let it sit for about an hour or so to let everything settle. Then, wipe down the interior with lysol or iso alcohol, and go to work 
This process may change depending on your design. Some people have open holes on their SAB which they plug up when not in use, while others have permanent gloves attached to the holes. You'll have to engineer something that works for your project with what you have available.
Hope this helped 
Yes this helped a lot. Just one more ting. From personal exprience do you think holes or permanent gloves is the way to go?
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thoraxx
Wizard


Registered: 12/27/13
Posts: 580
Loc: Bavaria
Last seen: 4 years, 10 months
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: Myconin]
#21778433 - 06/08/15 11:45 AM (8 years, 7 months ago) |
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I cant see how a flow hood is nessecary for anyone but large scale growers As a hobby grower it makes no difference if you have 10-20% contamination vs <5% or whatever
I just started a new grow, reviving spores from my last grow about a year ago Now agar work is propably the most contamination prone, but i just do everything in my kitchen without much more than an alcohol soaked rag and theres no problem really A couple of plates contam after inocc (never after pouring), but spore prints arent sterile in the first place and thats the reason for working with agar anyway I can toss those, as long as one plate survives with a healthy sector thats all i need, from there contaminations are minimal and i can finish working toward an isolated culture
I guess it depends on the location/condition of your workspace, but you should try out how much contamination you actually get with different approaches For me its much more convenient to just work out in the open, without long prep work, and just make enough petris and jars so i can toss out a few contams here and there vs making a big fuss with my SAB (which still doesnt rule out contams) and ultimately wind up with less because it kills my motivation In the time it takes me to get the box out of storage, wipe and spray everything, put the stuff in there and try to accomodate an open flame around alcohol soaked material, i can just put everything up on a clean surface, maybe wear a mask and gloves and be done with twice the work
Once you start to inocc with healthy agar, contam risk on your jars goes down significantly anyway
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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: thoraxx]
#21778464 - 06/08/15 11:52 AM (8 years, 7 months ago) |
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Thx for replies. Is there someone with a room fully devoted to mushroom growing and nothing else? Id like to see that.
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: ipappnasei]
#21778551 - 06/08/15 12:20 PM (8 years, 7 months ago) |
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I started off working in open air, then moved to a SAB when I got into agar. Purchased a flowhood a year later & don't regret it one bit. It just depends on what your needs are and how serious you are about the hobby.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: grainbrain]
#21778567 - 06/08/15 12:25 PM (8 years, 7 months ago) |
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Gloveboxes move air around more than a SAB. You flame sterilize outside the SAB anyway. Pretty sure people don't even use gloveboxes anymore.
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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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Re: at what point should one get a laminar flowhood or gloveboxes? [Re: GreenRabbit]
#21778638 - 06/08/15 12:47 PM (8 years, 7 months ago) |
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Quote:
GreenRabbit said: Gloveboxes move air around more than a SAB. You flame sterilize outside the SAB anyway. Pretty sure people don't even use gloveboxes anymore.
I fail to see how. Wouldnt you push in air without gloves ? Pls explain if you feel like it
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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: grainbrain]
#21778649 - 06/08/15 12:49 PM (8 years, 7 months ago) |
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Quote:
grainbrain said: I started off working in open air, then moved to a SAB when I got into agar. Purchased a flowhood a year later & don't regret it one bit. It just depends on what your needs are and how serious you are about the hobby.

Would you mind providing a purchase link for future use?
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: ipappnasei]
#21778663 - 06/08/15 12:53 PM (8 years, 7 months ago) |
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: ipappnasei]
#21778692 - 06/08/15 12:58 PM (8 years, 7 months ago) |
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When using a SAB, you need have all fans and a.c. in the house and rooms off. The more still the air in the house, the more still the air is in your SAB. When you put your hands into the SAB, air is allowed to exit the arm holes rather than be pushed around inside like a glove box would.
Even if you made a glove box air tight, the stretching of the gloves would stir up the air inside the box making it useless.
--------------------
AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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thoraxx
Wizard


Registered: 12/27/13
Posts: 580
Loc: Bavaria
Last seen: 4 years, 10 months
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: grainbrain]
#21778698 - 06/08/15 01:01 PM (8 years, 7 months ago) |
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Thats a decent price Im sure if you can spare the money its worth it for convenience of a sterile atmosphere than you can just turn on with a button, but just for growing psilos idk
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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: Grey]
#21778699 - 06/08/15 01:02 PM (8 years, 7 months ago) |
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Oh i see. Thx
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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: grainbrain]
#21778713 - 06/08/15 01:06 PM (8 years, 7 months ago) |
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Quote:
grainbrain said: Fungi Perfecti Series I 
Thats nice. Did you try if it works properly? Ive searched the forums here for selfmade ones and on every thread rogerrabbit was like " thats not a laminaor flow hood because..." :p
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: ipappnasei]
#21778742 - 06/08/15 01:12 PM (8 years, 7 months ago) |
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That and we flame sterilize our needles and tools. You can't have a flame in the glove box. Especially if it was doused in alcohol. You'd see a huge explosion in your face like some have lol. So you pull your arms out, flame the tool, then put it back in. It's all good
Edited by Mad Season (06/08/15 01:13 PM)
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: ipappnasei]
#21778750 - 06/08/15 01:14 PM (8 years, 7 months ago) |
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Quote:
ipappnasei said:
Quote:
grainbrain said: Fungi Perfecti Series I 
Thats nice. Did you try if it works properly? Ive searched the forums here for selfmade ones and on every thread rogerrabbit was like " thats not a laminaor flow hood because..." :p
It works properly.
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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: Mad Season]
#21778782 - 06/08/15 01:21 PM (8 years, 7 months ago) |
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Quote:
Mad Season said: That and we flame sterilize our needles and tools. You can't have a flame in the glove box. Especially if it was doused in alcohol. You'd see a huge explosion in your face like some have lol. So you pull your arms out, flame the tool, then put it back in. It's all good
Haha are there really people that did that?
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ipappnasei



Registered: 10/13/13
Posts: 145
Last seen: 6 years, 4 months
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Re: at what point should one get a laminar flowhood or gloveboxes?6 [Re: grainbrain]
#21778787 - 06/08/15 01:22 PM (8 years, 7 months ago) |
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Quote:
grainbrain said:
Quote:
ipappnasei said:
Quote:
grainbrain said: Fungi Perfecti Series I 
Thats nice. Did you try if it works properly? Ive searched the forums here for selfmade ones and on every thread rogerrabbit was like " thats not a laminaor flow hood because..." :p
It works properly. 
Amazing. Is this like your hobby or is it a business?
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