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Offlinecigam
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Registered: 11/20/14
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Loc: California
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First time working with agar
    #21774505 - 06/07/15 02:55 PM (8 years, 7 months ago)

I took a tissue sample from a nice cube steam. and 6 days later i have now done a transfer.




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Offlinelivelogikal
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Registered: 06/06/15
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Last seen: 1 month, 28 days
Re: First time working with agar [Re: cigam]
    #21774556 - 06/07/15 03:08 PM (8 years, 7 months ago)

Id go 8-9 more transfers before putting in grain.


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InvisiblebodhisattaMDiscordReddit
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Re: First time working with agar [Re: livelogikal]
    #21774653 - 06/07/15 03:35 PM (8 years, 7 months ago)

Quote:

livelogikal said:
Id go 8-9 more transfers before putting in grain.



:confused:


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Invisiblemush madness
absorbing everything
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Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: First time working with agar [Re: livelogikal]
    #21774665 - 06/07/15 03:37 PM (8 years, 7 months ago)

Quote:

cigam said:
I took a tissue sample from a nice cube steam. and 6 days later i have now done a transfer.








Nice work

Quote:

livelogikal said:
Id go 8-9 more transfers before putting in grain.




Umm no


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OfflinePinPornProducer
Buy the ticket, take the ride
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Registered: 08/23/14
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Re: First time working with agar [Re: mush madness]
    #21774676 - 06/07/15 03:39 PM (8 years, 7 months ago)

2-3 then grains if the culture is cleaned up by then


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Offlinelivelogikal
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Re: First time working with agar [Re: PinPornProducer]
    #21776845 - 06/08/15 12:30 AM (8 years, 7 months ago)

Depends on your goals. Isolation should be your only goal using agar (unless cleaning up). You will not get to that point in 3 transfers. Look at the growth... ummm I wouldnt be satisfied with that whispy ass growth, itll take 10 transfers to get that rigorous iso out of that myc!


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OfflineSksoul
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Registered: 10/31/14
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Re: First time working with agar [Re: livelogikal]
    #21776888 - 06/08/15 12:43 AM (8 years, 7 months ago)

Quote:

livelogikal said:
Depends on your goals. Isolation should be your only goal using agar (unless cleaning up). You will not get to that point in 3 transfers. Look at the growth... ummm I wouldnt be satisfied with that whispy ass growth, itll take 10 transfers to get that rigorous iso out of that myc!





Why should isolation be your only goal with agar? The main point of agar is to ensure you have clean cultures. If you isolate directly from MS, you could isolate a non-fruiting strain.


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InvisibleDirty Harry
Go ahead, make my day
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Registered: 06/06/15
Posts: 12
Loc: San Francisco
Re: First time working with agar [Re: Sksoul]
    #21777298 - 06/08/15 04:31 AM (8 years, 7 months ago)

Did you :sploosh: on that plate? LOL


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