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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: How do you keep your tyvek clean? [Re: mushpunx]
#21776278 - 06/07/15 09:47 PM (8 years, 7 months ago) |
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Quote:
mushpunx said: Well the reason Ive been using tyvek suit as of late is because (besides the fact I was given a bunch) it covers you up so well that, if the suit is clean you dont have to shower/ don freshly washed clothes when you work. But of course that only works if the suit is clean haha.
Like I said I normally shower and wear freshly washed scrubs, mask, gloves, hair net and tyvek sleeves.
I do my work in front of a flow hood. If its just agar plates or even agar to grain innoculations Im working on it isnt the biggest deal, but when it comes to G2G (especially into spawn bags) its easier for any nastys that happen to be on my sleeves to get blown around amd get into my work.
So yea, if Im scrubbing up and going through the works, but if my sleeves arent clean I could see it being a serious vector yea?
No worries, mush. Just straighten out the used sleeves, reinsert into gussets, and sterilize. BAM! Putting tyvek wrist sleeves in the Washer ruins their integrity. Used sleeves don't have to be spotless to be used as bag filters, because they get sterilized.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: How do you keep your tyvek clean? [Re: hamloaf]
#21776304 - 06/07/15 09:54 PM (8 years, 7 months ago) |
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Pasty you can't just leave it at "got really creative". What did you do lol
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: How do you keep your tyvek clean? [Re: maddchef]
#21776371 - 06/07/15 10:14 PM (8 years, 7 months ago) |
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Aha! So Loaf you sterilize your sleeves before you work in front of the hood , every time yea?2
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 Amateur Mycologists United AMU Q&A
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: How do you keep your tyvek clean? [Re: maddchef]
#21776415 - 06/07/15 10:23 PM (8 years, 7 months ago) |
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maddchef said: Pasty you can't just leave it at "got really creative". What did you do lol
Umm I really don't want to say. I will preface with it was a measure that was only used to allow me to make use of that particular batch of grain. I had over 400 lbs of it and couldn't bear to throw it out. But once I started to get creative I was able to use it everytime, no issues.
I sterilized it twice. First run would be for a hour to 90 min at 20 PSI. Second run would be done 12-18 hours later and was done for 120 min at 20 PSI. Worked like a charm after that.
I need to be clear. The spawn that was run only once always smelled good, recovered fairly well. Spawn run was a little longer usually but nothing extraordinary. Usually 10 days. But most of the time mold showed up either just before the first flush or just before the second. After I started to double the run it never happened again.
The proof was when I started to run Muda bottles. I went back to just one run with it. I would see all the little dark spots where the bacteria germinated in the grains outlined in the sub. The myc would overrun it but it was there. Because the colony remained unbroken, mold was unable to get in and cause problems. I started to double run the Muda bottles and never saw them again. I was convinced.
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mush madness
absorbing everything



Registered: 05/22/15
Posts: 252
Loc: Brazil
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Re: How do you keep your tyvek clean? [Re: Pastywhyte]
#21776451 - 06/07/15 10:34 PM (8 years, 7 months ago) |
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Quote:
Pastywhyte said:
Quote:
maddchef said: Pasty you can't just leave it at "got really creative". What did you do lol
Umm I really don't want to say. I will preface with it was a measure that was only used to allow me to make use of that particular batch of grain. I had over 400 lbs of it and couldn't bear to throw it out. But once I started to get creative I was able to use it everytime, no issues.
I sterilized it twice. First run would be for a hour to 90 min at 20 PSI. Second run would be done 12-18 hours later and was done for 120 min at 20 PSI. Worked like a charm after that.
I need to be clear. The spawn that was run only once always smelled good, recovered fairly well. Spawn run was a little longer usually but nothing extraordinary. Usually 10 days. But most of the time mold showed up either just before the first flush or just before the second. After I started to double the run it never happened again.
The proof was when I started to run Muda bottles. I went back to just one run with it. I would see all the little dark spots where the bacteria germinated in the grains outlined in the sub. The myc would overrun it but it was there. Because the colony remained unbroken, mold was unable to get in and cause problems. I started to double run the Muda bottles and never saw them again. I was convinced.
I have been having issues with my current batch of oats, I will try your idea of a double run.
Was it oats for you?
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Pastywhyte
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: How do you keep your tyvek clean? [Re: mush madness]
#21776462 - 06/07/15 10:37 PM (8 years, 7 months ago) |
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Yes it was oats. But I have had oats that also gave me zero issues. I wouldn't write oats off just cause they're oats. I would approach any grain with care and understand that there are vectors we are not able to see easily.
Do the double run. Worst case is that you're still where you started. Best case it works for ya.
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mush madness
absorbing everything



Registered: 05/22/15
Posts: 252
Loc: Brazil
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Re: How do you keep your tyvek clean? [Re: Pastywhyte]
#21776470 - 06/07/15 10:40 PM (8 years, 7 months ago) |
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Quote:
Pastywhyte said: Yes it was oats. But I have had oats that also gave me zero issues. I wouldn't write oats off just cause they're oats. I would approach any grain with care and understand that there are vectors we are not able to see easily.
Do the double run. Worst case is that you're still where you started. Best case it works for ya.
I hadn't had any issues with oats before and absolutely love them, just have a batch that are being difficult.
Thanks for your tip
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: How do you keep your tyvek clean? [Re: mush madness]
#21776755 - 06/07/15 11:55 PM (8 years, 7 months ago) |
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What's the benefit in sterilizing 12hours apart? Any reason to not do it back to back so core temp is held for longer?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Pastywhyte
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: How do you keep your tyvek clean? [Re: maddchef]
#21776775 - 06/07/15 11:59 PM (8 years, 7 months ago) |
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maddchef said: What's the benefit in sterilizing 12hours apart? Any reason to not do it back to back so core temp is held for longer?
Well the main reason was that a 4 hour cycle didn't work. I have some theories as to why. Not sure I'm ready to discuss them now. Its bedtime for me. Goodnight all.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: How do you keep your tyvek clean? [Re: Pastywhyte]
#21776811 - 06/08/15 12:16 AM (8 years, 7 months ago) |
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Party pooper. I believe I may have just about the same issue is why I inquire.
I have a clear Tupperware where you can clearly see on the bottom in a few places where bacteria is damaging my mycelium and reproducing.
It looks like a brown/rust colored spot and will soon spread and these grains were put through a 3.5hr run @15psi
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: How do you keep your tyvek clean? [Re: fearnoevil]
#21777031 - 06/08/15 01:46 AM (8 years, 7 months ago) |
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fearnoevil said: Honestly, I don't believe in wearing tyvek, and in fact, as unpleasant as the visualization may be, I do all my G2G and even cloning work in the buff, or at most with a clean pair of tighty whities, lol. Well that and a hair net and a freshly washed/bleached bandana covering my mouth, look kinda like a masked streaker, lol. I also work in open air, YES that's right, no glove box and DEFINITELY no laminar fucking flow hood either (what an immense waste of money, imo).
The main goal is to create an environment where there is ZERO air movement. To this end I have a small bare room, no carpet, all vents have been sealed, and the window is also completely sealed with tape. Also have weather-sealed around the door to the room and have one of those tubular seal things that slides under the door so that it's completely air-tight for all intensive purposes. Also make sure the AC/heating is turned off to minimize air movement within the house.
Then, about an hour before I start the process, I make sure all jars and work surfaces are wiped down with iso alky, and then I'll use about a 1/2 can of Lysol disenfectant to spray the entire room (and the bathroom too). I then leave and take a shower, use a lufa to scrub most of my upper body (great for your complexion, btw) towel dry with a freshly washed/bleached towel, put on slippers, a hair net and surgical gloves then go directly to my clean room. Open and close the door slowly, and then put on the sterilized bandana and tightly cover my mouth.
Sound like a lot of work? Well yes, somewhat, although it's not that bad and the trade off is that IT IS FAR AND AWAY THE EASIEST WAY TO ACTUALLY DO THE WORK, MUCH EASIER IN MY XP THEN WORKING WITH GLOVE/SHMUV BOX. So much easier to move around and see what you're doing as well. And whether you believe it or not, I've done countless G2G transfers as well as cloning and even some agar work this way, despite "experts" opinons to the contrary.
And sorry if this is a bit of a hi-jack, but to the OP's original question, you don't need a tyvek suit. Wipe down/wash your arms and hands with iso-alky, use latex gloves, also wiped with alky, and when doing G2G, wipe your spoon/utensile with alky between transfers and you should have no problem bringing contams into your workspace.
I'm guessing you don't have pets or kids and you don't live in a old house or trailer.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: How do you keep your tyvek clean? [Re: Pastywhyte]
#21777374 - 06/08/15 05:20 AM (8 years, 7 months ago) |
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Pastywhyte said: Procedure is more important. Don't matter how well you showered or laundered shit, if your stirring up air currents or moving incorrectly in front of the hood its gonna be a problem.
Clean tyvek sleeves in your bags that then get sterilized ain't that important. I don't use bags much but again, any contams on them are being killed. Now if the pores opened up too much while PCing maybe that's the issue but, even then I don't think its likely.
You seemed less than confident that your masters were truly clean. I would look there. Sounds to me like a bacteria issue, especially if your really loading a lot of bags in at once or using grain with a massive endospore count. Might need to really up them PC times. For me, even a longer PC cycle didn't solve my endospore issues with a certain batch of grain, I needed to get really creative.
I must have skipped over this post I didnt see it But I might have confused you, Im speaking of the tyvek that I wear on my arms, not that I use in my bags as filters.
Im fairly confident in my procedure, Im still new to bags so I *could* be a bit smoother but my motions are pretty smooth considering
What Im talking about is, I do my best to wipe everything down with ISO that goes in front of the hood, wipe my gloves down etc. But if Ive picked up mold spores on my sleeves between work (which is very likely in my house) it cant help to have them blowing around when Im doing G2G, smooth as though my hand motions might be 
So like Hamloaf sugguested since they dont wash well I guess thr bedt thing to do is run a few sets through a PC cycle in spawn bag or something and just pull thrm out as I need?
But Ive been trying to resolve this trich problem for a few months now, some of the improvements Ive made on procedure have made a real difference. So Im attacking this from all angles, and the bacteria angle has definitly caught my attention as well For all my efforts I should have a much lower contam rate.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: How do you keep your tyvek clean? [Re: mushpunx]
#21777414 - 06/08/15 05:47 AM (8 years, 7 months ago) |
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Quote:
mushpunx said: Aha! So Loaf you sterilize your sleeves before you work in front of the hood , every time yea?2
Correct. Recycled tyvek wrist sleeves, and substrates are sterilized at the same time. Vacuum seal created between bag gussets, and tyvek wrist sleeves is only broken in the direct downstream of the flowhood.
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mushpunx
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Registered: 04/20/14
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Re: How do you keep your tyvek clean? [Re: hamloaf]
#21777511 - 06/08/15 06:39 AM (8 years, 7 months ago) |
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Ahh SQuote:
hamloaf said:
Quote:
mushpunx said: Aha! So Loaf you sterilize your sleeves before you work in front of the hood , every time yea?2
Correct. Recycled tyvek wrist sleeves, and substrates are sterilized at the same time. Vacuum seal created between bag gussets, and tyvek wrist sleeves is only broken in the direct downstream of the flowhood.

Ah. See yea I have a couple seperate pairs Ive just been keeping in gallon ziplocks. When I inoculate spawn bags I just pull out the wrist sleeves and set them aside, never occured to me to then wear them while Im doing work as theyve just been nicely cleaned
I could see that being a source of *some*of my problems, working in sleeves that might not be tottally clean..
I never move anythinh between the hood and open work. But who knows what could be blowing around off my sleeves espdcially in my house
Its not such an issue working with agar because my arms are kept pretty far away but with bags your elbows are moving around quite a bit manuevering
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (06/08/15 07:00 AM)
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
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Re: How do you keep your tyvek clean? [Re: mushpunx]
#21777753 - 06/08/15 08:33 AM (8 years, 7 months ago) |
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Yea, wearing dirty sleeves in the direct downstream of the flowhood will blow contaminates into the sterile air of direct downstream of flowhood. Solution, have you a pair of wrist sleeves exclusively designated for the wearing during inoculations. Also, be sure that no grain kernels are left on the sleeves, and that the sleeves are DRY before enclosing in a ziplock bag for storage.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: How do you keep your tyvek clean? [Re: hamloaf]
#21777816 - 06/08/15 08:54 AM (8 years, 7 months ago) |
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Yeah this thread kinda went sideways, sorry op.
Just put your work sleeves in a jar and PC them, then stick in a zip lock bag when not in use to keep junk from landing on them.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: How do you keep your tyvek clean? [Re: maddchef]
#21778108 - 06/08/15 10:17 AM (8 years, 7 months ago) |
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Hah
No its a good thread, good discussion.
Never occurred to me to be cleaning these sleeves like and I feel like thiz should make a good bit of difference.
Learned a couple other things to be lookimg at aa well.
By the time I start getting 100% sucess rate Ill be a freaking rock star
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 Amateur Mycologists United AMU Q&A
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fearnoevil
Stranger than you think ;?D

Registered: 06/07/15
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Re: How do you keep your tyvek clean? [Re: mushpunx]
#21800504 - 06/13/15 02:07 AM (8 years, 7 months ago) |
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Insanemike, lol, no I don't, although my house dates to the early '70s, but I understand with the kids especially.
Pasteywhyte, I'm not certain that a second sterilization is necessary, at least to my understanding. Why not simply increase the initial duration to 120 minutes rather than 90? If you are PCing at 20 psi with temps around 259f, from my research I don't think there are many bacteria/endospores if any that will survive for that length of time at those temperatures.
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Pastywhyte
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Registered: 09/15/12
Posts: 37,810
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Re: How do you keep your tyvek clean? [Re: fearnoevil]
#21801135 - 06/13/15 08:26 AM (8 years, 7 months ago) |
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Proof of the pudding is in the eating. 4 hours in one run didn't work. One hour for the first and 90 min for the second did. I have my theories why. More research is being done but at the end of the day what grows mushrooms works and what grows mold does not.
I must make it clear that this is not a standard procedure for me. Most of my grains are run for 100min and that is enough. The double run was a measure needed for a single batch of grain. Its not even the type of grain at play. Just a single instance. But I had hundreds of pounds of it and can't bear waste.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
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Re: How do you keep your tyvek clean? [Re: mushpunx]
#21801161 - 06/13/15 08:36 AM (8 years, 7 months ago) |
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Quote:
mushpunx said:
I could see that being a source of *some*of my problems, working in sleeves that might not be tottally clean..
Only takes one fuck up no matter how big, or small said fuck up is to ruin a culture, and thus an entire grow as a result. Don't need to tell you that sometimes infections to the mushroom culture go undetected. Tighten up your sterile technique from all angels you can possibly dream of, start with clean cultures, and the rest will be history.
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