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InvisibleHeisencybin
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Does it matter if you accidentally skip a hole pf tek jar inoculation?
    #21773450 - 06/07/15 10:15 AM (8 years, 7 months ago)

I may have missed count or accidently skipped a hole while inoculating my jars. So I only have 3/4 holes inoculated or maybe 4/4 but did the same hole twice? Would that matter either? If I inoculated the same hole twice on accident?


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OfflineMacMerdin
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: Heisencybin]
    #21773484 - 06/07/15 10:25 AM (8 years, 7 months ago)

You should be fine if everything else is good.

Meaning a skipped hole or double innoc is the least of your worries in this hobbie.  :thumbup:


--------------------
MacMerdin's Simple Pan-Cakes



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OfflineMajickMuffin
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: MacMerdin]
    #21773507 - 06/07/15 10:31 AM (8 years, 7 months ago)

Fuck yea it matters, if you only did 3/4 holes and missed 1/4, no spores will germinate.
(sarcasm)


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OfflineGrim767
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: MajickMuffin]
    #21773560 - 06/07/15 10:45 AM (8 years, 7 months ago)

Everything should be fine


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflineFreeWorldOrder
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: Grim767]
    #21774095 - 06/07/15 01:31 PM (8 years, 7 months ago)

Will be just fine. Will just take a little longer for those sides to fill in with mycelium.


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK


Edited by FreeWorldOrder (06/07/15 01:31 PM)


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OfflineGrimley
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: FreeWorldOrder]
    #21774165 - 06/07/15 01:48 PM (8 years, 7 months ago)

Usually I only make one hole in the middle, any try to get the spore solution as close to the center of jar substrate as possible. Works good for me, and dosent effect the time rate.


--------------------

Routine is lethal!


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OfflineTNK
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: Grimley]
    #21774306 - 06/07/15 02:17 PM (8 years, 7 months ago)

Quote:

Grimley said:
Usually I only make one hole in the middle, any try to get the spore solution as close to the center of jar substrate as possible. Works good for me, and dosent effect the time rate.




The idea behind inoculating closer to the jar wall is to be able to watch for contams, but if your confident with your sterilization than the middle works just as good as any--


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Edited by TNK (02/22/22 22:22 PM)


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OfflineGrimley
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: TNK]
    #21774350 - 06/07/15 02:23 PM (8 years, 7 months ago)

Usually I only make one hole in the middle, any try to get the spore solution as close to the center of jar substrate as possible. Works good for me, and dosent effect the time rate.
Quote:

TheNatureKid said:
Quote:

Grimley said:
Usually I only make one hole in the middle, any try to get the spore solution as close to the center of jar substrate as possible. Works good for me, and dosent effect the time rate.




The idea behind inoculating closer to the jar wall is to be able to watch for contams, but if your confident with your sterilization than the middle works just as good as any--




yep, but as you say, I'm very confident in my sterilization, and I don't watch my jars grow like I did in the beginning.  haven't had a contam in a very long time.


--------------------

Routine is lethal!


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Offlinenwalker248
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: Grimley]
    #21774410 - 06/07/15 02:34 PM (8 years, 7 months ago)

best case out of this very small issue is that you double inoculated a whole, because it would mean that you got more spores into the jar overall. But as others have said it really doesn't matter too much and will still colonize.


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Invisibleoontribe
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: nwalker248]
    #21774445 - 06/07/15 02:41 PM (8 years, 7 months ago)

The idea of 4 holes is to speed up colonization.


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OfflineGrimley
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: oontribe]
    #21774468 - 06/07/15 02:48 PM (8 years, 7 months ago)

Quote:

oontribe said:
The idea of 4 holes is to speed up colonization.




don't see any difference in 4 holes or 1, but that's me! I use the same amount,of solution tough... Just wanna state that skipping a hole isn't anything to fear...


--------------------

Routine is lethal!


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OfflineTNK
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: oontribe]
    #21774482 - 06/07/15 02:50 PM (8 years, 7 months ago)

Quote:

oontribe said:
The idea of 4 holes is to speed up colonization.




That is sort'of true--

I have yet to experiment with growth speeds of 1 hole versus 4, technically when you innoc 4 holes you are starting four seperate MYC growth's rather than only 1


--------------------
Edited by TNK (02/22/22 22:22 PM)


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OfflineFreeWorldOrder
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: oontribe]
    #21774792 - 06/07/15 04:00 PM (8 years, 7 months ago)

Quote:

oontribe said:
The idea of 4 holes is to speed up colonization.




Exactly...

And that's just grade school common sense. If you are starting mycelium on 4 different sides of container, of course it will colonize faster.

I don't understand why some people have such a hard time with that concept.

Another thing, I wouldn't inoculate in the center of jar either.

The whole purpose of letting the solution run down the sides of the jar is so the mycelium starts on the sides and can thus be monitored for contams.

I seldom do a PF-Tek anymore, or use spore syringes for that matter. (more into cloning & isolation of strains on agar, agar wedge inoculated grain jars, G2G, and monotubs or trays.

But there is absolutely nothing wrong with doing cakes. The method is damn near fool-proof as long as you follow the TEK. And is where most of us have started out learning the basics.

I would stick to the TEK and do the four 1/8th holes for best results and fastest colonization times... simple as that.


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK


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OfflineGrimley
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: FreeWorldOrder]
    #21774866 - 06/07/15 04:13 PM (8 years, 7 months ago)

Quote:

FreeWorldOrder said:
Quote:

oontribe said:
The idea of 4 holes is to speed up colonization.




Exactly...

And that's just grade school common sense. If you are starting mycelium on 4 different sides of container, of course it will colonize faster.

I don't understand why some people have such a hard time with that concept.

Another thing, I wouldn't inoculate in the center of jar either.

The whole purpose of letting the solution run down the sides of the jar is so the mycelium starts on the sides and can thus be monitored for contams.

I seldom do a PF-Tek anymore, or use spore syringes for that matter. (more into cloning & isolation of strains on agar, agar wedge inoculated grain jars, G2G, and monotubs or trays.

But there is absolutely nothing wrong with doing cakes. The method is damn near fool-proof as long as you follow the TEK. And is where most of us have started out learning the basics.

I would stick to the TEK and do the four 1/8th holes for best results and fastest colonization times... simple as that.




yeah your absolutely right. But when inoculating 30 jars, its easier with one point inject, just my "bad" habbit, and I never recommended it. Stick with 4hole tek. Forget about my shit, my point was, does it matter that much skipping one single hole... No.


--------------------

Routine is lethal!


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InvisibleHeisencybin
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: Grimley]
    #21777529 - 06/08/15 06:45 AM (8 years, 7 months ago)

Thanks for the info guys! I can rest easy for the next 3-5 weeks now :smile:


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OfflineMajickMuffin
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: FreeWorldOrder]
    #21777617 - 06/08/15 07:28 AM (8 years, 7 months ago)

Quote:

FreeWorldOrder said:
Quote:

oontribe said:
The idea of 4 holes is to speed up colonization.




Exactly...

And that's just grade school common sense. If you are starting mycelium on 4 different sides of container, of course it will colonize faster.

I don't understand why some people have such a hard time with that concept.

Another thing, I wouldn't inoculate in the center of jar either.

The whole purpose of letting the solution run down the sides of the jar is so the mycelium starts on the sides and can thus be monitored for contams.

I seldom do a PF-Tek anymore, or use spore syringes for that matter. (more into cloning & isolation of strains on agar, agar wedge inoculated grain jars, G2G, and monotubs or trays.

But there is absolutely nothing wrong with doing cakes. The method is damn near fool-proof as long as you follow the TEK. And is where most of us have started out learning the basics.

I would stick to the TEK and do the four 1/8th holes for best results and fastest colonization times... simple as that.




Your saying the purpose of innoculating on sides is for monitoring whether it has contams? You would figure that shit out either way. If you start in the middle it will reach to the sides, and if its contaminated you will still see it.


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Offlinenwalker248
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: MajickMuffin]
    #21777628 - 06/08/15 07:35 AM (8 years, 7 months ago)

I think what he means is you dont have to wait for the mycelium/contam to reach the edge, and so can get on with throwing them away faster.


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OfflineMajickMuffin
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: nwalker248]
    #21777830 - 06/08/15 08:58 AM (8 years, 7 months ago)

Quote:

nwalker248 said:
I think what he means is you dont have to wait for the mycelium/contam to reach the edge, and so can get on with throwing them away faster.



I get that, but if your confident that your syringe is clean, which is most likely, that shoulsnt even be a factor. Imo


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OfflineLoveNaborFuckHater
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: TNK]
    #21777992 - 06/08/15 09:47 AM (8 years, 7 months ago)

Quote:

TheNatureKid said:
Quote:

oontribe said:
The idea of 4 holes is to speed up colonization.




That is sort'of true--

I have yet to experiment with growth speeds of 1 hole versus 4, technically when you innoc 4 holes you are starting four seperate MYC growth's rather than only 1



I've never noticed a difference in speed, alot of time I'll innoc only 2 holes on jars that have 4 holes and some I only made two holes.  Alot of times I'll have one or two innoc points that never actually grow so I just cut back to save spore solution. I've had two innoc points out-grow 4 points by many days.


--------------------
"They told me drugs were bad, oh man, oh man, they had me fooled"


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OfflineFreeWorldOrder
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Re: Does it matter if you accidentally skip a hole pf tek jar inoculation? [Re: MajickMuffin]
    #21778736 - 06/08/15 01:11 PM (8 years, 7 months ago)

Quote:

MajickMuffin said:
Quote:

nwalker248 said:
I think what he means is you dont have to wait for the mycelium/contam to reach the edge, and so can get on with throwing them away faster.



I get that, but if your confident that your syringe is clean, which is most likely, that shoulsnt even be a factor. Imo




One can never be confident that any syringe is clean. After all, it's made from a print taken from a fruit that has been exposed to dirty ass air for days...lol.

Too many "if"'s to form any solid basis, imo.


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK


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