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OfflineMr.Brain
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P. Azurescens indoor cultivation log
    #21769870 - 06/06/15 10:35 AM (8 years, 7 months ago)

Hi everyone,

I just decided to fill another box with some mycelium and write down a little grow log.
Incubator:
http://www.exo-terra.com/en/products/incubator.php

Setup for the box:
- 1cm perlite on the ground
- in the middle on it about 4*4*1cm mycelium
- the middle layer is a ~3-4cm mix of sawdust and small wood chips
- the top layer is 1cm soil



The temperature in the incubator is 22-24°C

I'll post the next update, as soon as I can see mycelium anywhere at the side of the box.

If you have any questions or something like that, feel free to ask :wink:

So far,
Mr.Brain


Edited by Mr.Brain (06/06/15 10:35 AM)


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Invisiblemustangbob3
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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21769878 - 06/06/15 10:37 AM (8 years, 7 months ago)

1 question why perlite on the bottom?

your room temp would also incubate that fine :thumbup:


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Edited by mustangbob3 (06/06/15 10:38 AM)


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: mustangbob3]
    #21769893 - 06/06/15 10:39 AM (8 years, 7 months ago)

From time to time I had problems with too much water on the ground in those boxes. When I put in the wood to early for example. I just thought of it as an "insurance", no water is collecting on the ground.

At the moment it's pretty warm here, the incubator has a cooling function already ^^


Edited by Mr.Brain (06/06/15 10:41 AM)


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Invisiblemustangbob3
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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21769907 - 06/06/15 10:44 AM (8 years, 7 months ago)

good luck :popcorn:

how are you planning to fruit? :smile:


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Edited by mustangbob3 (06/06/15 10:46 AM)


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: mustangbob3]
    #21769934 - 06/06/15 10:49 AM (8 years, 7 months ago)

At the moment my plan would be to wait, until the soil is covered with mycelium, then drop the temperature to 8-10°C and see what happens :laugh:
Oh yeah and of course wrap the box in aluminium foil so it only gets light from the upside

Another edit :laugh:
For the cubensis I grew before, I had the feeling the optional blue led light seemed to have a positive effect on the growth rate, I'll watch out, if I can observe something similar here.


Edited by Mr.Brain (06/06/15 10:52 AM)


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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21770009 - 06/06/15 11:08 AM (8 years, 7 months ago)

Quote:

Oh yeah and of course wrap the box in aluminium foil so it only gets light from the upside



it doesn't matter where the light hits.

temperature doesn't cause Azurs to fruit plenty of indoor grows have been tried in fridges and other temperature controlled methods with 0 results or one or two fruits. soil microbes and symbiotic relationships are required for the fruiting as far as most people are concerned.


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Invisiblemustangbob3
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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21770024 - 06/06/15 11:11 AM (8 years, 7 months ago)

when you say soil on top was it sterile soil or a good living garden loam?

like bodhisatta said generally its thought a living soil or at least the presence of the right bacteria is what helps fruiting as they seem to live in symbiosis with something in the ground.

whats your thoughts on an extended dunk along with cool temps to help start fruiting?

like copying heavy rains.


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Edited by mustangbob3 (06/06/15 11:24 AM)


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: mustangbob3]
    #21770038 - 06/06/15 11:16 AM (8 years, 7 months ago)

Quote:

mustangbob3 said:
when you say soil on top was it sterile soil or a good living garden loam?




I have it from a mushroom cultivation online shop, they sell it as casing layer soil, it's enriched with calcium carbonate. That's all the info I have on the soil.


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Invisiblemustangbob3
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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21770050 - 06/06/15 11:20 AM (8 years, 7 months ago)

you can prob bet that its sterile then..
like bodhi said lots have tried and succeeded but only minimal fruits.
there seems to be a piece of the puzzle still missing and most think its beneficial bacteria or fungi.

not sure what tests have been done to find out what is responsible.
nowadays glomus intradices and other such soil bacteria is easy to source but there is so many to try!



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Edited by mustangbob3 (06/06/15 11:24 AM)


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: mustangbob3]
    #21770067 - 06/06/15 11:23 AM (8 years, 7 months ago)

Quote:

mustangbob3 said:
you can prob bet that its sterile then..
like bohdi said lots have tried and succeeded but only minimal fruits.
there seems to be a piece of the puzzle still missing and most think its beneficial bacteria or fungi.




Well, I'll just see what happens, if nothing happens, I may remove just the soil layer from the top, get into the woods, grab some earth and smaller stuff near trees, use this as a new top layer and see what happens then :laugh:


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Invisiblemustangbob3
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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21770079 - 06/06/15 11:26 AM (8 years, 7 months ago)

i would leave it to colonise and then try the outdoor soil as a casing when it come to fruiting :thumbup:

you never know :smile:


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: mustangbob3]
    #21770090 - 06/06/15 11:30 AM (8 years, 7 months ago)

Quote:

mustangbob3 said:
i would leave it to colonise and then try the outdoor soil as a casing when it come to fruiting :thumbup:

you never know :smile:




I mean, whatever is needed, it can't be tooo special, can it? As far as I read almost anybody could grow them in a bed of woodchips in the own garden, if temp is right in your area. So almost anybody must have it in the own garden :laugh:


Edited by Mr.Brain (06/06/15 11:31 AM)


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Invisiblemustangbob3
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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21770096 - 06/06/15 11:33 AM (8 years, 7 months ago)

yes outdoors can be simpler than cubes imo

whatever they may need to fruit is quite common in nature it seems.:smile:

i been  doing my first grow of azzies for the last few months and all i have done is chuck is some good garden loam ( a good handful nothing drastic) in hope its enough. :smile:

heres a link if your interested -
http://www.shroomery.org/forums/showflat.php/Number/21728044/page/3


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Edited by mustangbob3 (06/06/15 11:37 AM)


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Re: P. Azurescens indoor cultivation log [Re: mustangbob3]
    #21770630 - 06/06/15 02:06 PM (8 years, 7 months ago)

I definitely believe the presence of beneficial bacteria or a similar symbiosis is required too.  A friend in the UK had a very prolific outdoor Psi cyanescens grow, then decided to try fruiting indoors.

He dug up a clump of colonized wood chips (hence beneficial bacteria already present) and attempted fruiting in a modified wine chiller; primordia DID form but never matured, only aborted.  Besides the bacteria/soil symbiosis, I definitely think they require strong(er) FAE and wetness common outdoors in the fall where Psi. azurescens/cyanescens are indigenous.

I also currently have my first Psi azurescens in progress.


http://www.shroomery.org/forums/showflat.php/Number/21119350


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: mycomattie]
    #21771856 - 06/06/15 08:29 PM (8 years, 7 months ago)

Quote:

mycomattie said:
I definitely believe the presence of beneficial bacteria or a similar symbiosis is required too.  A friend in the UK had a very prolific outdoor Psi cyanescens grow, then decided to try fruiting indoors.

He dug up a clump of colonized wood chips (hence beneficial bacteria already present) and attempted fruiting in a modified wine chiller; primordia DID form but never matured, only aborted.  Besides the bacteria/soil symbiosis, I definitely think they require strong(er) FAE and wetness common outdoors in the fall where Psi. azurescens/cyanescens are indigenous.

I also currently have my first Psi azurescens in progress.


http://www.shroomery.org/forums/showflat.php/Number/21119350




Since the room temp here is always higher or lower, than the temp in the incubator the fan of the incubator will turn on like every 1-2 mins for about 1 min, so air is exchanged almost permanently

€dit:
What about the water ph value? Ever compared the water you use, with the rain water? Does this maybe make a difference?


Edited by Mr.Brain (06/06/15 08:57 PM)


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Re: P. Azurescens indoor cultivation log [Re: bodhisatta] * 1
    #21771968 - 06/06/15 08:57 PM (8 years, 7 months ago)

Quote:

bodhisatta said:
Quote:

Oh yeah and of course wrap the box in aluminium foil so it only gets light from the upside



it doesn't matter where the light hits.

temperature doesn't cause Azurs to fruit plenty of indoor grows have been tried in fridges and other temperature controlled methods with 0 results or one or two fruits. soil microbes and symbiotic relationships are required for the fruiting as far as most people are concerned.




Aren't azures similar to Ps cyan? 'Cause I've fruited cyans on wood chips in a converted fridge with no problems.  Slow as shit, but that goes with the territory. :lol:


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: PrimalSoup]
    #21772893 - 06/07/15 05:24 AM (8 years, 7 months ago)

Quote:

PrimalSoup said:
Quote:

bodhisatta said:
Quote:

Oh yeah and of course wrap the box in aluminium foil so it only gets light from the upside



it doesn't matter where the light hits.

temperature doesn't cause Azurs to fruit plenty of indoor grows have been tried in fridges and other temperature controlled methods with 0 results or one or two fruits. soil microbes and symbiotic relationships are required for the fruiting as far as most people are concerned.




Aren't azures similar to Ps cyan? 'Cause I've fruited cyans on wood chips in a converted fridge with no problems.  Slow as shit, but that goes with the territory. :lol:




How did you modify the fridge? How was the result?

Oh, another question, has anyone yet used bark mulch as top layer? I saw this in a pdf, describing how to lay a mushroom bed (Azurescens) in the garden.


Edited by Mr.Brain (06/07/15 07:15 AM)


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21773314 - 06/07/15 09:30 AM (8 years, 7 months ago)

I don't know if I missed this yesterday, or if this is just going really fast ^^


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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21773564 - 06/07/15 10:46 AM (8 years, 7 months ago)

:popcorn:


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Re: P. Azurescens indoor cultivation log [Re: nobody83]
    #21774008 - 06/07/15 01:09 PM (8 years, 7 months ago)

Quote:

nobody83 said:
:popcorn:




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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21774059 - 06/07/15 01:22 PM (8 years, 7 months ago)

Quote:

Mr.Brain said:
Quote:

PrimalSoup said:
Quote:

bodhisatta said:
Quote:

Oh yeah and of course wrap the box in aluminium foil so it only gets light from the upside



it doesn't matter where the light hits.

temperature doesn't cause Azurs to fruit plenty of indoor grows have been tried in fridges and other temperature controlled methods with 0 results or one or two fruits. soil microbes and symbiotic relationships are required for the fruiting as far as most people are concerned.




Aren't azures similar to Ps cyan? 'Cause I've fruited cyans on wood chips in a converted fridge with no problems.  Slow as shit, but that goes with the territory. :lol:




How did you modify the fridge? How was the result?

Oh, another question, has anyone yet used bark mulch as top layer? I saw this in a pdf, describing how to lay a mushroom bed (Azurescens) in the garden.




I put a light in it and maybe jiggered the thermostat so I could get it to the temp I wanted.  It was in the garage when we moved in so I'm not sure how well it was working before that. :lol:

This was on cased wood chips, BTW, but nothing special about it at all except the temps.


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: PrimalSoup]
    #21777640 - 06/08/15 07:40 AM (8 years, 7 months ago)

Well, seems like this is really going fast :smile: Already three spots where I can see the mycelium.
Next Update will be in a few days, when you see some more, than those little spots.



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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21777709 - 06/08/15 08:11 AM (8 years, 7 months ago)

:thumbup: :popcorn:


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OfflineMr.Brain
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Re: P. Azurescens indoor cultivation log [Re: mustangbob3]
    #21779441 - 06/08/15 03:56 PM (8 years, 7 months ago)

I also have a container here, with 3/4 complete mycelium.
Today I saw some contaminations at the corners, so I cut off the contaminations, chopped the rest of the mycelium to pieces and refilled the box with woodchips / sawdust.
After that, I filled another Box with perlite and used some single (clean) inoculated wood chips I cut off before. But for the top layer I used some bark mulch out of the garden. I will post some pictures, when it gets interesting.
I'm primary interested, if bark mulch is maybe the better top layer, since some manuals for a wood based mushroom bed recommend it, so lets see whats happening :smile:


Edited by Mr.Brain (06/08/15 04:00 PM)


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Re: P. Azurescens indoor cultivation log [Re: Mr.Brain]
    #21779626 - 06/08/15 04:56 PM (8 years, 7 months ago)

:super: Definitely gonna be keeping tabs on your grow!


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Re: P. Azurescens indoor cultivation log [Re: fan5]
    #21781542 - 06/09/15 01:56 AM (8 years, 7 months ago)

im interested how the different casing/ top layers preform :smile:


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Re: P. Azurescens indoor cultivation log [Re: mustangbob3]
    #21781892 - 06/09/15 06:41 AM (8 years, 7 months ago)

Even my first outdoor flush of azures and cyans wasn't established enough so it was a weak flush definitely require some extra goodies from outdoors! :mushroom2:


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Re: P. Azurescens indoor cultivation log [Re: Eclipse3130]
    #21781979 - 06/09/15 07:33 AM (8 years, 7 months ago)

Quote:

Eclipse3130 said:
Even my first outdoor flush of azures and cyans wasn't established enough so it was a weak flush definitely require some extra goodies from outdoors! :mushroom2:




Well, I'm really looking forward to see what will happen in my incubator :smile:
If nothing happens, I guess I'll put the mycelium into the fridge until fall and then place it outside.

But from what I saw, one of the main differences to most indoor Azu grows is, that I not only have control over the temperature, but also a permanent air exchange, because compared to a normal fridge the incubator isn't a closed system and has a vent on the backside.

So I guess, I will try different casing layers until it works and if not ... it's going into the fridge.


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