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OfflineYaMoonSun
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Registered: 10/23/14
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Bread Thread
    #21769237 - 06/06/15 07:39 AM (8 years, 7 months ago)

After making myself a sandwich, only the heel remained in the bread-bag.
Rather than let it go to waste, I thought I'd try a grown-up version of the experiment I did in 2nd grade.



I washed my hands, grabbed a zip-lock bag, and quickly transferred the bread into the zip-lock whilst holding my breath; Sealed the zip-lock half way, gave it three sprays from my misting bottle, and closed the remaining zip-lock. Then I sterilized the needle of my spore syringe, and cooled the needled with a bit of spore solution, opened the zip-lock just ever-so-slightly, and inoculated probably less than 1cc (ml) into the bag.

I don't really know how I'm going to fruit it, but for now, let's focus on colonization. It's really going to be a race of myc vs trich. Probably would work better with LC or LI.


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Invisiblemaddchef
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Re: Bread Thread [Re: YaMoonSun]
    #21769243 - 06/06/15 07:41 AM (8 years, 7 months ago)

Why bother sterilizing your needle to put spores solution onto a dirty piece of bread? Lol


--------------------
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All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

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InvisibleZombi3
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Re: Bread Thread [Re: YaMoonSun]
    #21769245 - 06/06/15 07:42 AM (8 years, 7 months ago)

I foresee moldy bread coming.

I would have just made toast, you dont eat the end slice?


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OfflineMajickMuffin
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Re: Bread Thread [Re: maddchef]
    #21769247 - 06/06/15 07:43 AM (8 years, 7 months ago)

What Brand of Bread is that?

I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better :shrug:


Edited by MajickMuffin (06/06/15 07:44 AM)


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OfflineYaMoonSun
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Re: Bread Thread [Re: maddchef]
    #21769249 - 06/06/15 07:44 AM (8 years, 7 months ago)

Loving the optimism, lol; I just like to follow standard procedures - Even on not-so-sterile substrates. To point in further contributing to contaminates.


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Re: Bread Thread [Re: YaMoonSun]
    #21769260 - 06/06/15 07:49 AM (8 years, 7 months ago)

Who wants to make a bet? No offence OP... LOL
Will this innoculated bread totally contam?
You may choose only one


Votes accepted from (06/06/15 05:49 AM) to (No end specified)
You must vote before you can view the results of this poll



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OfflineYaMoonSun
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Re: Bread Thread [Re: Zombi3]
    #21769265 - 06/06/15 07:51 AM (8 years, 7 months ago)

Quote:

MajickMuffin said:
What Brand of Bread is that?

I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better :shrug:



SaraLee "Healthy Multi-Grain Bread"



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OfflineMajickMuffin
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Re: Bread Thread [Re: YaMoonSun]
    #21769271 - 06/06/15 07:52 AM (8 years, 7 months ago)

Well, why didnt you add 'Has the possibility to grow successful mycelium, but will likely contaminate'

That makes most sense to me. Theres always a chance, but no its not likely at all.

Quote:

YaMoonSun said:
Quote:

MajickMuffin said:
What Brand of Bread is that?

I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better :shrug:



SaraLee "Healthy Multi-Grain Bread"







Cool, Keep us updated with results.


Edited by MajickMuffin (06/06/15 07:52 AM)


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InvisibleZombi3
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Re: Bread Thread [Re: MajickMuffin]
    #21769277 - 06/06/15 07:54 AM (8 years, 7 months ago)

I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.

Definetily keep us updated :toast:

Good luck OP!


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OfflineYaMoonSun
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Re: Bread Thread [Re: YaMoonSun]
    #21769279 - 06/06/15 07:54 AM (8 years, 7 months ago)

Quote:

MajickMuffin said:
Well, why didnt you add 'Has the possibility to grow successful mycelium, but will likely contaminate'

That makes most sense to me. Theres always a chance, but no its not likely at all.

Cool, Keep us updated with results.





Quote:

YaMoonSun said:
It's really going to be a race of myc vs trich. Probably would work better with LC or LI.




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Invisiblemustangbob3
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Re: Bread Thread [Re: YaMoonSun]
    #21769282 - 06/06/15 07:55 AM (8 years, 7 months ago)

dont waste the end slice the crusts the best bit especialy for toast!! :kingtard:


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OfflineMajickMuffin
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Re: Bread Thread [Re: Zombi3]
    #21769283 - 06/06/15 07:56 AM (8 years, 7 months ago)

Quote:

Zombi3 said:
I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.

Definetily keep us updated :toast:

Good luck OP!



Best way to do it would be to make homemade bread and seperate it into a sterile environment directly after cooking, while its still cooling.


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InvisibleZombi3
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Re: Bread Thread [Re: MajickMuffin]
    #21769291 - 06/06/15 07:58 AM (8 years, 7 months ago)

Quote:

MajickMuffin said:
Quote:

Zombi3 said:
I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.

Definetily keep us updated :toast:

Good luck OP!



Best way to do it would be to make homemade bread and seperate it into a sterile environment directly after cooking, while its still cooling.



Yes I agree. Plus home made bread is where its at. I hate store bought bread. Get a bread machine and never look back. :thumbup:


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Invisiblemustangbob3
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Re: Bread Thread [Re: MajickMuffin]
    #21769293 - 06/06/15 07:58 AM (8 years, 7 months ago)

wheres the adventure in that lol


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Invisiblemaddchef
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Re: Bread Thread [Re: MajickMuffin]
    #21769297 - 06/06/15 07:59 AM (8 years, 7 months ago)

This will totally work since the op stated "held my breath".


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineYaMoonSun
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Re: Bread Thread [Re: Zombi3]
    #21769300 - 06/06/15 08:00 AM (8 years, 7 months ago)

Ingredient List:
  • Whole Wheat Flour
  • Wheat
  • Rye
  • Barley
  • Oats
  • Corn
  • Millet
  • Sugar
  • Water
  • Wheat Gluten
  • Oat Fiber
  • Yeast
  • Cultured Wheat Flour
  • Soybean Oil
  • Flaxseed
  • Distilled Vinegar
  • Wheat Brain
  • Calcium Sulfite
  • "Enzymes"
  • Ascorbic Acid
  • Milk
  • Soy Flour


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OfflineYaMoonSun
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Re: Bread Thread [Re: maddchef]
    #21769306 - 06/06/15 08:00 AM (8 years, 7 months ago)

:lol:
I really can't wait to see what happens.
Promise I won't hold my breath through the whole colonization xD


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Invisibleazur
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Re: Bread Thread [Re: Zombi3] * 1
    #21769309 - 06/06/15 08:01 AM (8 years, 7 months ago)

I predict penicillium


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OfflineMajickMuffin
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Re: Bread Thread [Re: YaMoonSun]
    #21769311 - 06/06/15 08:02 AM (8 years, 7 months ago)

Quote:

YaMoonSun said:
Ingredient List:
  • Whole Wheat Flour
  • Wheat
  • Rye
  • Barley
  • Oats
  • Corn
  • Millet
  • Sugar
  • Water
  • Wheat Gluten
  • Oat Fiber
  • Yeast
  • Cultured Wheat Flour
  • Soybean Oil
  • Flaxseed
  • Distilled Vinegar
  • Wheat Brain
  • Calcium Sulfite
  • "Enzymes"
  • Ascorbic Acid
  • Milk
  • Soy Flour




Yea but if Sarah Lee touched it you know dat bitch sprinkeld some kind of shit in there.


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OfflineFreeWorldOrder
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Re: Bread Thread [Re: YaMoonSun]
    #21769321 - 06/06/15 08:07 AM (8 years, 7 months ago)

It will probably just mold... but no harm in trying if you have the extra spores right.


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OfflineYaMoonSun
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Re: Bread Thread [Re: FreeWorldOrder]
    #21769328 - 06/06/15 08:09 AM (8 years, 7 months ago)

Yeah, that's people kept saying in threads I was browsing, but I've got time to kill, and OPs from previous threads didn't even make an attempt. :cheers:


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InvisibleZombi3
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Re: Bread Thread [Re: FreeWorldOrder]
    #21769329 - 06/06/15 08:09 AM (8 years, 7 months ago)

Quote:

FreeWorldOrder said:
no harm in trying if you have the extra spores right.



Correct. I have failed many times. No harm in trying.


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OfflineMajickMuffin
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Re: Bread Thread [Re: Zombi3]
    #21769375 - 06/06/15 08:23 AM (8 years, 7 months ago)

Quote:

Zombi3 said:
Quote:

FreeWorldOrder said:
no harm in trying if you have the extra spores right.



Correct. I have failed many times. No harm in trying.



But did you see ANY mycelium growth?


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Invisibleazur
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Re: Bread Thread [Re: YaMoonSun]
    #21769380 - 06/06/15 08:24 AM (8 years, 7 months ago)

Quote:

YaMoonSun said:
Yeah, that's people kept saying in threads I was browsing, but I've got time to kill, and OPs from previous threads didn't even make an attempt. :cheers:



Kill time until there's no time to kill. Not cool.


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FALL IN LOVE WITH LC
FOTTSE!!!
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InvisibleZombi3
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Re: Bread Thread [Re: MajickMuffin]
    #21769387 - 06/06/15 08:26 AM (8 years, 7 months ago)

Ummmm i saw mycelium growth in about %2 of the silly "out of the box" experiments like this that I tried. But that %2 was on stuff I had PC'd. Ive never ever seen myc grow on experiments like this that werent were not PC'd. Save for some mycelium I did manage to grow out of a random flower pot, I think I harvested like 3-5g cubensis from a draceanna reflexa pot, but then the myc contammd and killed my draceanna, so....


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InvisiblePastywhyteMDiscord
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Re: Bread Thread [Re: Zombi3] * 1
    #21769393 - 06/06/15 08:29 AM (8 years, 7 months ago)

Quote:

Zombi3 said:
I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.





I haven't had bread mold on me in some time, I was considering that it was some kind of chemical additive, like benomyl or something. In any case it would very likely prevent the cube spores from germinating.


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OfflineMajickMuffin
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Re: Bread Thread [Re: Pastywhyte]
    #21769399 - 06/06/15 08:31 AM (8 years, 7 months ago)

You guys should help me 0out real fast

http://www.shroomery.org/forums/showflat.php/Number/21769364


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OfflineNcogneato
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Re: Bread Thread [Re: Zombi3]
    #21769414 - 06/06/15 08:35 AM (8 years, 7 months ago)

I can see why you are getting some ridicule but being on this forum for any length of time will quickly desensitize you of negativity and snobbery. I would have tried to sterilize the bread though. I don't know how well it would stand up to a PC but that may have been a better way to start. I am guessing that the mean green will show up rather quickly and spoil your party. If you have any other mycology projects going on you may want to isolate that bread to keep spore contamination to a minimum.


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OfflineYaMoonSun
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Re: Bread Thread [Re: Pastywhyte]
    #21769438 - 06/06/15 08:43 AM (8 years, 7 months ago)

What brand are ya dealing with? I've seen trich colonizing this SaraLee in the past; When people would ignore the last piece of bread for a week or two.


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InvisibleZombi3
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Re: Bread Thread [Re: YaMoonSun]
    #21769444 - 06/06/15 08:45 AM (8 years, 7 months ago)

Quote:

YaMoonSun said:
What brand are ya dealing with? I've seen trich colonizing this SaraLee in the past; When people would ignore the last piece of bread for a week or two.



A week or two is how long mycelium will take to grow if you used MS syringe...

Now youve added water for that trich, its not going to take a week or two for it to show up this time.


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Re: Bread Thread [Re: YaMoonSun]
    #21769447 - 06/06/15 08:47 AM (8 years, 7 months ago)

Bread will need to be sterilized if its to have a chance. If mold won't germinate on it chances are nothing will. If the cube spores can germinate that means competitors can too. End.


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OfflineYaMoonSun
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Re: Bread Thread [Re: Ncogneato]
    #21769448 - 06/06/15 08:47 AM (8 years, 7 months ago)

If I recall correctly, (from the 2nd grade, lol) I had a "clean" piece of bread, and one that I tossed into a sandbox, and they were each in difference baggies. The sandbox baggie took about a week and a half to colonize and then it was tossed. The "clean" bread has a little trich on the corners were it was handled. I don't recall washing my hands. This was YEARS ago though, maybe the teacher made me wash my hands and I don't recall.


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OfflineYaMoonSun
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Re: Bread Thread [Re: Pastywhyte]
    #21769459 - 06/06/15 08:50 AM (8 years, 7 months ago)

I wouldn't doubt the chances of this experiment working are about the same as getting hit by lightning, but I like my chances nevertheless. At least there's a chance. Am going to let it run it's course, and if it fails, I may try again with sterilized bread (dunno how I'm going to pull that off) or a LC.


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InvisiblePastywhyteMDiscord
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Re: Bread Thread [Re: YaMoonSun]
    #21769487 - 06/06/15 08:58 AM (8 years, 7 months ago)

Just to be clear, even if it did produce a fruit it still wouldn't be an experiment. It would be at best a proof of concept, nothing more. The odds of this working are far less than being hit by lightning tho. Compared to being hit by lightning or abducted by aliens this would be a long shot.


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Invisiblemustangbob3
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Re: Bread Thread [Re: YaMoonSun]
    #21769500 - 06/06/15 09:03 AM (8 years, 7 months ago)

mircowave the bread in a baggie!
that will sterilise to give you a window to colonise prob:thumbup:

just bread will turn soggy in microwave dont add h20!!


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InvisiblePastywhyteMDiscord
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Re: Bread Thread [Re: mustangbob3]
    #21769530 - 06/06/15 09:11 AM (8 years, 7 months ago)

The best way to pull off a grow of this nature would be to have a fresh piece of bread placed into a pp5 sandwich container. Ziploc makes lots of em. Then add a filtered hole for GE and sterilize in a PC. You would want to avoid liquid inoculate so I would either use grains sprinkled over the bread or a few agar wedges. It would certainly be an interesting novelty.


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Invisiblemustangbob3
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Re: Bread Thread [Re: Pastywhyte]
    #21769562 - 06/06/15 09:23 AM (8 years, 7 months ago)

:thumbup:


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OfflineYaMoonSun
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Re: Bread Thread [Re: mustangbob3]
    #21819897 - 06/17/15 05:28 PM (8 years, 7 months ago)

Ten days later and no signs of myc or contaminates, going to give it a solid 30 days before I toss it though. Go vote on that one guy's poll on the first page. :super:


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InvisibleZombi3
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Re: Bread Thread [Re: YaMoonSun]
    #21819977 - 06/17/15 05:53 PM (8 years, 7 months ago)

That one guy has a name bro :wink:


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OfflineYaMoonSun
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Re: Bread Thread [Re: Zombi3]
    #21820022 - 06/17/15 06:01 PM (8 years, 7 months ago)

I was too lazy to go back to the first page to check, sorry :stoned:


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Re: Bread Thread [Re: YaMoonSun]
    #21820128 - 06/17/15 06:22 PM (8 years, 7 months ago)

I'm only busting your balls cuz I made the poll :lol:


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OfflineYaMoonSun
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Re: Bread Thread [Re: Zombi3]
    #21943633 - 07/14/15 07:43 PM (8 years, 6 months ago)

Nearly thirty days later and no signs of life from any fungi/bacteria/mold. I don't think I wanna buy this bread anymore :shrug:


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