|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
Bread Thread
#21769237 - 06/06/15 07:39 AM (8 years, 7 months ago) |
|
|
After making myself a sandwich, only the heel remained in the bread-bag. Rather than let it go to waste, I thought I'd try a grown-up version of the experiment I did in 2nd grade.

I washed my hands, grabbed a zip-lock bag, and quickly transferred the bread into the zip-lock whilst holding my breath; Sealed the zip-lock half way, gave it three sprays from my misting bottle, and closed the remaining zip-lock. Then I sterilized the needle of my spore syringe, and cooled the needled with a bit of spore solution, opened the zip-lock just ever-so-slightly, and inoculated probably less than 1cc (ml) into the bag.
I don't really know how I'm going to fruit it, but for now, let's focus on colonization. It's really going to be a race of myc vs trich. Probably would work better with LC or LI.
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
|
Why bother sterilizing your needle to put spores solution onto a dirty piece of bread? Lol
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
|
I foresee moldy bread coming.
I would have just made toast, you dont eat the end slice?
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
|
Re: Bread Thread [Re: maddchef]
#21769247 - 06/06/15 07:43 AM (8 years, 7 months ago) |
|
|
What Brand of Bread is that?
I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better
Edited by MajickMuffin (06/06/15 07:44 AM)
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
Re: Bread Thread [Re: maddchef]
#21769249 - 06/06/15 07:44 AM (8 years, 7 months ago) |
|
|
Loving the optimism, lol; I just like to follow standard procedures - Even on not-so-sterile substrates. To point in further contributing to contaminates.
|
Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
|
Who wants to make a bet? No offence OP... LOL
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
Re: Bread Thread [Re: Zombi3]
#21769265 - 06/06/15 07:51 AM (8 years, 7 months ago) |
|
|
Quote:
MajickMuffin said: What Brand of Bread is that?
I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better 
SaraLee "Healthy Multi-Grain Bread"
|
MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
|
|
Well, why didnt you add 'Has the possibility to grow successful mycelium, but will likely contaminate'
That makes most sense to me. Theres always a chance, but no its not likely at all.
Quote:
YaMoonSun said:
Quote:
MajickMuffin said: What Brand of Bread is that?
I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better 
SaraLee "Healthy Multi-Grain Bread"

Cool, Keep us updated with results.
Edited by MajickMuffin (06/06/15 07:52 AM)
|
Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
|
I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.
Definetily keep us updated 
Good luck OP!
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
|
Quote:
MajickMuffin said: Well, why didnt you add 'Has the possibility to grow successful mycelium, but will likely contaminate'
That makes most sense to me. Theres always a chance, but no its not likely at all.
Cool, Keep us updated with results.
Quote:
YaMoonSun said: It's really going to be a race of myc vs trich. Probably would work better with LC or LI.
|
mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
|
|
dont waste the end slice the crusts the best bit especialy for toast!!
--------------------
|
MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
|
Re: Bread Thread [Re: Zombi3]
#21769283 - 06/06/15 07:56 AM (8 years, 7 months ago) |
|
|
Quote:
Zombi3 said: I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.
Definetily keep us updated 
Good luck OP!
Best way to do it would be to make homemade bread and seperate it into a sterile environment directly after cooking, while its still cooling.
|
Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
|
Quote:
MajickMuffin said:
Quote:
Zombi3 said: I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.
Definetily keep us updated 
Good luck OP!
Best way to do it would be to make homemade bread and seperate it into a sterile environment directly after cooking, while its still cooling.
Yes I agree. Plus home made bread is where its at. I hate store bought bread. Get a bread machine and never look back.
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
|
|
wheres the adventure in that lol
--------------------
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
|
This will totally work since the op stated "held my breath".
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
Re: Bread Thread [Re: Zombi3]
#21769300 - 06/06/15 08:00 AM (8 years, 7 months ago) |
|
|
Ingredient List:
- Whole Wheat Flour
- Wheat
- Rye
- Barley
- Oats
- Corn
- Millet
- Sugar
- Water
- Wheat Gluten
- Oat Fiber
- Yeast
- Cultured Wheat Flour
- Soybean Oil
- Flaxseed
- Distilled Vinegar
- Wheat Brain
- Calcium Sulfite
- "Enzymes"
- Ascorbic Acid
- Milk
- Soy Flour
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
Re: Bread Thread [Re: maddchef]
#21769306 - 06/06/15 08:00 AM (8 years, 7 months ago) |
|
|
 I really can't wait to see what happens. Promise I won't hold my breath through the whole colonization xD
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: Bread Thread [Re: Zombi3] 1
#21769309 - 06/06/15 08:01 AM (8 years, 7 months ago) |
|
|
I predict penicillium
|
MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
|
|
Quote:
YaMoonSun said: Ingredient List:
- Whole Wheat Flour
- Wheat
- Rye
- Barley
- Oats
- Corn
- Millet
- Sugar
- Water
- Wheat Gluten
- Oat Fiber
- Yeast
- Cultured Wheat Flour
- Soybean Oil
- Flaxseed
- Distilled Vinegar
- Wheat Brain
- Calcium Sulfite
- "Enzymes"
- Ascorbic Acid
- Milk
- Soy Flour
Yea but if Sarah Lee touched it you know dat bitch sprinkeld some kind of shit in there.
|
FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 11 hours
|
|
It will probably just mold... but no harm in trying if you have the extra spores right.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
|
Yeah, that's people kept saying in threads I was browsing, but I've got time to kill, and OPs from previous threads didn't even make an attempt.
|
Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
|
Quote:
FreeWorldOrder said: no harm in trying if you have the extra spores right.
Correct. I have failed many times. No harm in trying.
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
|
Re: Bread Thread [Re: Zombi3]
#21769375 - 06/06/15 08:23 AM (8 years, 7 months ago) |
|
|
Quote:
Zombi3 said:
Quote:
FreeWorldOrder said: no harm in trying if you have the extra spores right.
Correct. I have failed many times. No harm in trying.
But did you see ANY mycelium growth?
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
|
Quote:
YaMoonSun said: Yeah, that's people kept saying in threads I was browsing, but I've got time to kill, and OPs from previous threads didn't even make an attempt. 
Kill time until there's no time to kill. Not cool.
|
Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
|
Ummmm i saw mycelium growth in about %2 of the silly "out of the box" experiments like this that I tried. But that %2 was on stuff I had PC'd. Ive never ever seen myc grow on experiments like this that werent were not PC'd. Save for some mycelium I did manage to grow out of a random flower pot, I think I harvested like 3-5g cubensis from a draceanna reflexa pot, but then the myc contammd and killed my draceanna, so....
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Bread Thread [Re: Zombi3] 1
#21769393 - 06/06/15 08:29 AM (8 years, 7 months ago) |
|
|
Quote:
Zombi3 said: I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.
I haven't had bread mold on me in some time, I was considering that it was some kind of chemical additive, like benomyl or something. In any case it would very likely prevent the cube spores from germinating.
|
MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
|
|
|
Ncogneato
Patriot



Registered: 04/27/15
Posts: 228
Loc: Wisco
Last seen: 7 years, 11 months
|
Re: Bread Thread [Re: Zombi3]
#21769414 - 06/06/15 08:35 AM (8 years, 7 months ago) |
|
|
I can see why you are getting some ridicule but being on this forum for any length of time will quickly desensitize you of negativity and snobbery. I would have tried to sterilize the bread though. I don't know how well it would stand up to a PC but that may have been a better way to start. I am guessing that the mean green will show up rather quickly and spoil your party. If you have any other mycology projects going on you may want to isolate that bread to keep spore contamination to a minimum.
-------------------- Psilocybin.........the poor man's Cancun.
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
|
What brand are ya dealing with? I've seen trich colonizing this SaraLee in the past; When people would ignore the last piece of bread for a week or two.
|
Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
|
Quote:
YaMoonSun said: What brand are ya dealing with? I've seen trich colonizing this SaraLee in the past; When people would ignore the last piece of bread for a week or two.
A week or two is how long mycelium will take to grow if you used MS syringe...
Now youve added water for that trich, its not going to take a week or two for it to show up this time.
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
Bread will need to be sterilized if its to have a chance. If mold won't germinate on it chances are nothing will. If the cube spores can germinate that means competitors can too. End.
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
|
If I recall correctly, (from the 2nd grade, lol) I had a "clean" piece of bread, and one that I tossed into a sandbox, and they were each in difference baggies. The sandbox baggie took about a week and a half to colonize and then it was tossed. The "clean" bread has a little trich on the corners were it was handled. I don't recall washing my hands. This was YEARS ago though, maybe the teacher made me wash my hands and I don't recall.
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
|
I wouldn't doubt the chances of this experiment working are about the same as getting hit by lightning, but I like my chances nevertheless. At least there's a chance. Am going to let it run it's course, and if it fails, I may try again with sterilized bread (dunno how I'm going to pull that off) or a LC.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
Just to be clear, even if it did produce a fruit it still wouldn't be an experiment. It would be at best a proof of concept, nothing more. The odds of this working are far less than being hit by lightning tho. Compared to being hit by lightning or abducted by aliens this would be a long shot.
|
mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
|
|
mircowave the bread in a baggie! that will sterilise to give you a window to colonise prob
just bread will turn soggy in microwave dont add h20!!
--------------------
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
The best way to pull off a grow of this nature would be to have a fresh piece of bread placed into a pp5 sandwich container. Ziploc makes lots of em. Then add a filtered hole for GE and sterilize in a PC. You would want to avoid liquid inoculate so I would either use grains sprinkled over the bread or a few agar wedges. It would certainly be an interesting novelty.
|
mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
|
|
--------------------
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
|
Ten days later and no signs of myc or contaminates, going to give it a solid 30 days before I toss it though. Go vote on that one guy's poll on the first page.
|
Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
|
That one guy has a name bro
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
Re: Bread Thread [Re: Zombi3]
#21820022 - 06/17/15 06:01 PM (8 years, 7 months ago) |
|
|
I was too lazy to go back to the first page to check, sorry
|
Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
|
|
I'm only busting your balls cuz I made the poll
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
|
Re: Bread Thread [Re: Zombi3]
#21943633 - 07/14/15 07:43 PM (8 years, 6 months ago) |
|
|
Nearly thirty days later and no signs of life from any fungi/bacteria/mold. I don't think I wanna buy this bread anymore
|
|