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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Bread Thread
    #21769237 - 06/06/15 07:39 AM (8 years, 7 months ago)

After making myself a sandwich, only the heel remained in the bread-bag.
Rather than let it go to waste, I thought I'd try a grown-up version of the experiment I did in 2nd grade.



I washed my hands, grabbed a zip-lock bag, and quickly transferred the bread into the zip-lock whilst holding my breath; Sealed the zip-lock half way, gave it three sprays from my misting bottle, and closed the remaining zip-lock. Then I sterilized the needle of my spore syringe, and cooled the needled with a bit of spore solution, opened the zip-lock just ever-so-slightly, and inoculated probably less than 1cc (ml) into the bag.

I don't really know how I'm going to fruit it, but for now, let's focus on colonization. It's really going to be a race of myc vs trich. Probably would work better with LC or LI.


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Invisiblemaddchef
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Re: Bread Thread [Re: YaMoonSun]
    #21769243 - 06/06/15 07:41 AM (8 years, 7 months ago)

Why bother sterilizing your needle to put spores solution onto a dirty piece of bread? Lol


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleZombi3
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Re: Bread Thread [Re: YaMoonSun]
    #21769245 - 06/06/15 07:42 AM (8 years, 7 months ago)

I foresee moldy bread coming.

I would have just made toast, you dont eat the end slice?


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OfflineMajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
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Re: Bread Thread [Re: maddchef]
    #21769247 - 06/06/15 07:43 AM (8 years, 7 months ago)

What Brand of Bread is that?

I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better :shrug:


Edited by MajickMuffin (06/06/15 07:44 AM)


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
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Re: Bread Thread [Re: maddchef]
    #21769249 - 06/06/15 07:44 AM (8 years, 7 months ago)

Loving the optimism, lol; I just like to follow standard procedures - Even on not-so-sterile substrates. To point in further contributing to contaminates.


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InvisibleZombi3
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Re: Bread Thread [Re: YaMoonSun]
    #21769260 - 06/06/15 07:49 AM (8 years, 7 months ago)

Who wants to make a bet? No offence OP... LOL
Will this innoculated bread totally contam?
You may choose only one


Votes accepted from (06/06/15 05:49 AM) to (No end specified)
You must vote before you can view the results of this poll



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OfflineYaMoonSun
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Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Bread Thread [Re: Zombi3]
    #21769265 - 06/06/15 07:51 AM (8 years, 7 months ago)

Quote:

MajickMuffin said:
What Brand of Bread is that?

I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better :shrug:



SaraLee "Healthy Multi-Grain Bread"



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OfflineMajickMuffin
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Re: Bread Thread [Re: YaMoonSun]
    #21769271 - 06/06/15 07:52 AM (8 years, 7 months ago)

Well, why didnt you add 'Has the possibility to grow successful mycelium, but will likely contaminate'

That makes most sense to me. Theres always a chance, but no its not likely at all.

Quote:

YaMoonSun said:
Quote:

MajickMuffin said:
What Brand of Bread is that?

I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better :shrug:



SaraLee "Healthy Multi-Grain Bread"







Cool, Keep us updated with results.


Edited by MajickMuffin (06/06/15 07:52 AM)


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InvisibleZombi3
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Re: Bread Thread [Re: MajickMuffin]
    #21769277 - 06/06/15 07:54 AM (8 years, 7 months ago)

I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.

Definetily keep us updated :toast:

Good luck OP!


--------------------
Youve Met With A Terrible Fate, Havent You?

Click here to enter this weeks Ban Lottery!!
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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Bread Thread [Re: YaMoonSun]
    #21769279 - 06/06/15 07:54 AM (8 years, 7 months ago)

Quote:

MajickMuffin said:
Well, why didnt you add 'Has the possibility to grow successful mycelium, but will likely contaminate'

That makes most sense to me. Theres always a chance, but no its not likely at all.

Cool, Keep us updated with results.





Quote:

YaMoonSun said:
It's really going to be a race of myc vs trich. Probably would work better with LC or LI.




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Invisiblemustangbob3
Mad Myrmecologist
I'm a teapot


Registered: 10/15/14
Posts: 1,685
Re: Bread Thread [Re: YaMoonSun]
    #21769282 - 06/06/15 07:55 AM (8 years, 7 months ago)

dont waste the end slice the crusts the best bit especialy for toast!! :kingtard:


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OfflineMajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
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Re: Bread Thread [Re: Zombi3]
    #21769283 - 06/06/15 07:56 AM (8 years, 7 months ago)

Quote:

Zombi3 said:
I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.

Definetily keep us updated :toast:

Good luck OP!



Best way to do it would be to make homemade bread and seperate it into a sterile environment directly after cooking, while its still cooling.


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InvisibleZombi3
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Re: Bread Thread [Re: MajickMuffin]
    #21769291 - 06/06/15 07:58 AM (8 years, 7 months ago)

Quote:

MajickMuffin said:
Quote:

Zombi3 said:
I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.

Definetily keep us updated :toast:

Good luck OP!



Best way to do it would be to make homemade bread and seperate it into a sterile environment directly after cooking, while its still cooling.



Yes I agree. Plus home made bread is where its at. I hate store bought bread. Get a bread machine and never look back. :thumbup:


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Youve Met With A Terrible Fate, Havent You?

Click here to enter this weeks Ban Lottery!!
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Invisiblemustangbob3
Mad Myrmecologist
I'm a teapot


Registered: 10/15/14
Posts: 1,685
Re: Bread Thread [Re: MajickMuffin]
    #21769293 - 06/06/15 07:58 AM (8 years, 7 months ago)

wheres the adventure in that lol


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Invisiblemaddchef
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Re: Bread Thread [Re: MajickMuffin]
    #21769297 - 06/06/15 07:59 AM (8 years, 7 months ago)

This will totally work since the op stated "held my breath".


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Bread Thread [Re: Zombi3]
    #21769300 - 06/06/15 08:00 AM (8 years, 7 months ago)

Ingredient List:
  • Whole Wheat Flour
  • Wheat
  • Rye
  • Barley
  • Oats
  • Corn
  • Millet
  • Sugar
  • Water
  • Wheat Gluten
  • Oat Fiber
  • Yeast
  • Cultured Wheat Flour
  • Soybean Oil
  • Flaxseed
  • Distilled Vinegar
  • Wheat Brain
  • Calcium Sulfite
  • "Enzymes"
  • Ascorbic Acid
  • Milk
  • Soy Flour


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Bread Thread [Re: maddchef]
    #21769306 - 06/06/15 08:00 AM (8 years, 7 months ago)

:lol:
I really can't wait to see what happens.
Promise I won't hold my breath through the whole colonization xD


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Invisibleazur
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Re: Bread Thread [Re: Zombi3] * 1
    #21769309 - 06/06/15 08:01 AM (8 years, 7 months ago)

I predict penicillium


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OfflineMajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
Re: Bread Thread [Re: YaMoonSun]
    #21769311 - 06/06/15 08:02 AM (8 years, 7 months ago)

Quote:

YaMoonSun said:
Ingredient List:
  • Whole Wheat Flour
  • Wheat
  • Rye
  • Barley
  • Oats
  • Corn
  • Millet
  • Sugar
  • Water
  • Wheat Gluten
  • Oat Fiber
  • Yeast
  • Cultured Wheat Flour
  • Soybean Oil
  • Flaxseed
  • Distilled Vinegar
  • Wheat Brain
  • Calcium Sulfite
  • "Enzymes"
  • Ascorbic Acid
  • Milk
  • Soy Flour




Yea but if Sarah Lee touched it you know dat bitch sprinkeld some kind of shit in there.


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OfflineFreeWorldOrder
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Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA Flag
Last seen: 8 days, 7 hours
Re: Bread Thread [Re: YaMoonSun]
    #21769321 - 06/06/15 08:07 AM (8 years, 7 months ago)

It will probably just mold... but no harm in trying if you have the extra spores right.


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"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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