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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Bread Thread
#21769237 - 06/06/15 07:39 AM (8 years, 7 months ago) |
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After making myself a sandwich, only the heel remained in the bread-bag. Rather than let it go to waste, I thought I'd try a grown-up version of the experiment I did in 2nd grade.

I washed my hands, grabbed a zip-lock bag, and quickly transferred the bread into the zip-lock whilst holding my breath; Sealed the zip-lock half way, gave it three sprays from my misting bottle, and closed the remaining zip-lock. Then I sterilized the needle of my spore syringe, and cooled the needled with a bit of spore solution, opened the zip-lock just ever-so-slightly, and inoculated probably less than 1cc (ml) into the bag.
I don't really know how I'm going to fruit it, but for now, let's focus on colonization. It's really going to be a race of myc vs trich. Probably would work better with LC or LI.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Why bother sterilizing your needle to put spores solution onto a dirty piece of bread? Lol
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
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I foresee moldy bread coming.
I would have just made toast, you dont eat the end slice?
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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Re: Bread Thread [Re: maddchef]
#21769247 - 06/06/15 07:43 AM (8 years, 7 months ago) |
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What Brand of Bread is that?
I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better
Edited by MajickMuffin (06/06/15 07:44 AM)
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: Bread Thread [Re: maddchef]
#21769249 - 06/06/15 07:44 AM (8 years, 7 months ago) |
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Loving the optimism, lol; I just like to follow standard procedures - Even on not-so-sterile substrates. To point in further contributing to contaminates.
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Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
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Who wants to make a bet? No offence OP... LOL
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: Bread Thread [Re: Zombi3]
#21769265 - 06/06/15 07:51 AM (8 years, 7 months ago) |
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Quote:
MajickMuffin said: What Brand of Bread is that?
I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better 
SaraLee "Healthy Multi-Grain Bread"
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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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Well, why didnt you add 'Has the possibility to grow successful mycelium, but will likely contaminate'
That makes most sense to me. Theres always a chance, but no its not likely at all.
Quote:
YaMoonSun said:
Quote:
MajickMuffin said: What Brand of Bread is that?
I always toast the end slice, actually I always toast bread, my sandwiches are always toasted. Its just fucking better 
SaraLee "Healthy Multi-Grain Bread"

Cool, Keep us updated with results.
Edited by MajickMuffin (06/06/15 07:52 AM)
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Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
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I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.
Definetily keep us updated 
Good luck OP!
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Quote:
MajickMuffin said: Well, why didnt you add 'Has the possibility to grow successful mycelium, but will likely contaminate'
That makes most sense to me. Theres always a chance, but no its not likely at all.
Cool, Keep us updated with results.
Quote:
YaMoonSun said: It's really going to be a race of myc vs trich. Probably would work better with LC or LI.
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mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
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dont waste the end slice the crusts the best bit especialy for toast!!
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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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Re: Bread Thread [Re: Zombi3]
#21769283 - 06/06/15 07:56 AM (8 years, 7 months ago) |
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Quote:
Zombi3 said: I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.
Definetily keep us updated 
Good luck OP!
Best way to do it would be to make homemade bread and seperate it into a sterile environment directly after cooking, while its still cooling.
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Zombi3
Bella Ciao!!




Registered: 01/11/13
Posts: 27,086
Loc: Bat Country
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Quote:
MajickMuffin said:
Quote:
Zombi3 said: I bet theres a bunch of weird preservatives in that bread that will kill your spores. But Im no expert.
Definetily keep us updated 
Good luck OP!
Best way to do it would be to make homemade bread and seperate it into a sterile environment directly after cooking, while its still cooling.
Yes I agree. Plus home made bread is where its at. I hate store bought bread. Get a bread machine and never look back.
-------------------- You’ve Met With A Terrible Fate, Haven’t You?
Click here to enter this weeks Ban Lottery!! In Crust We Trust
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mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
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wheres the adventure in that lol
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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This will totally work since the op stated "held my breath".
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: Bread Thread [Re: Zombi3]
#21769300 - 06/06/15 08:00 AM (8 years, 7 months ago) |
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Ingredient List:
- Whole Wheat Flour
- Wheat
- Rye
- Barley
- Oats
- Corn
- Millet
- Sugar
- Water
- Wheat Gluten
- Oat Fiber
- Yeast
- Cultured Wheat Flour
- Soybean Oil
- Flaxseed
- Distilled Vinegar
- Wheat Brain
- Calcium Sulfite
- "Enzymes"
- Ascorbic Acid
- Milk
- Soy Flour
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: Bread Thread [Re: maddchef]
#21769306 - 06/06/15 08:00 AM (8 years, 7 months ago) |
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 I really can't wait to see what happens. Promise I won't hold my breath through the whole colonization xD
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Bread Thread [Re: Zombi3] 1
#21769309 - 06/06/15 08:01 AM (8 years, 7 months ago) |
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I predict penicillium
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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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Quote:
YaMoonSun said: Ingredient List:
- Whole Wheat Flour
- Wheat
- Rye
- Barley
- Oats
- Corn
- Millet
- Sugar
- Water
- Wheat Gluten
- Oat Fiber
- Yeast
- Cultured Wheat Flour
- Soybean Oil
- Flaxseed
- Distilled Vinegar
- Wheat Brain
- Calcium Sulfite
- "Enzymes"
- Ascorbic Acid
- Milk
- Soy Flour
Yea but if Sarah Lee touched it you know dat bitch sprinkeld some kind of shit in there.
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 7 hours
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It will probably just mold... but no harm in trying if you have the extra spores right.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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