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mromgwtf
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Registered: 06/06/15
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Last seen: 6 years, 2 months
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Growing scletoria without spores? (Directly from an existing truffle)
#21768723 - 06/06/15 02:30 AM (8 years, 7 months ago) |
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Hey. I've recently bought 15 grams of psilocybe atlantis. I've made a tea with it with tons of lemon juice. I took it together with my friend. It was our first time, it was quite amazing.
I left out a small single truffle for the purpose of cultivation. How would I go about growing it? I've read quite a lot about scletoria cultivation, but all of those techniques use spores. How can I grow it from an existing truffle? I can't imagine how I'd put it inside of a jar that has been sterilized without contaminating it. Can somebody give me some pointers? Thanks in advance.
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
#21768816 - 06/06/15 03:18 AM (8 years, 7 months ago) |
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A-G-A-R
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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YaMoonSun
The Double Standard


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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Buck513]
#21768848 - 06/06/15 03:33 AM (8 years, 7 months ago) |
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mromgwtf
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Buck513]
#21768903 - 06/06/15 04:14 AM (8 years, 7 months ago) |
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Quote:
YaMoonSun said: This is a pretty good read; Good luck. http://www.shroomery.org/forums/showflat.php/Number/20833312/fpart/1
So if I prepare a proper agar petri dish, and drop the stone in there, will it grow?
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Edited by mromgwtf (06/06/15 04:15 AM)
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MUSHmania
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
#21768904 - 06/06/15 04:16 AM (8 years, 7 months ago) |
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Quote:
mromgwtf said:
Quote:
YaMoonSun said: This is a pretty good read; Good luck. http://www.shroomery.org/forums/showflat.php/Number/20833312/fpart/1
So if I prepare a proper agar petri dish, and drop the stone in there, will it grow?
Take a tissue sample and clone to agar
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oontribe

Registered: 01/14/15
Posts: 3,570
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: MUSHmania]
#21768919 - 06/06/15 04:33 AM (8 years, 7 months ago) |
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i think you take a tissue usually from the stem if they are fruits and you drop it in the agar and they start to grow. dunno about stones!
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: oontribe]
#21768992 - 06/06/15 05:13 AM (8 years, 7 months ago) |
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You can clone a stone by taking a tissue sample from a stone. Plenty of people do that
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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mromgwtf
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Registered: 06/06/15
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Buck513]
#21769003 - 06/06/15 05:23 AM (8 years, 7 months ago) |
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Quote:
Buck513 said:

You can clone a stone by taking a tissue sample from a stone. Plenty of people do that
So basically take a piece of the inside of the stone with a syringe or anything, and drop it in to a prepared jar of rice/ryegrass seed/whatever?
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
#21769021 - 06/06/15 05:33 AM (8 years, 7 months ago) |
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No. Take a tissue sample and put it on AGAR. Transfer a couple times to clean it up if need be. You should have a strictly stone producing culture.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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oontribe

Registered: 01/14/15
Posts: 3,570
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Buck513]
#21769052 - 06/06/15 05:58 AM (8 years, 7 months ago) |
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yea i think it work the same as fruits.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: oontribe]
#21769400 - 06/06/15 08:31 AM (8 years, 7 months ago) |
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Rip stone in half, take sterile scalpel and cut out a meaty piece of it. A place that hadn't touched open air. Inside a still air box, as sterile as you can be. Drop the flesh on a petri dish of agar. I'd probably make a few dishes to be safe. Transfer when clean and if you get a clean culture, tiger drop it into grain jars.
Edited by Mad Season (06/06/15 08:33 AM)
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mromgwtf
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Mad Season]
#21769642 - 06/06/15 09:45 AM (8 years, 7 months ago) |
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What about growing it in a jar full of agar with nutrients? Agar seems so nice because you can see through it, and so you are fully aware of what's going on in there.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
#21769691 - 06/06/15 09:55 AM (8 years, 7 months ago) |
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Yes. You can use wide mouth half pints like you do for pf tek, or pp5 containers.
http://www.shroomery.org/forums/showflat.php/Number/19208976
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Mad Season]
#21769798 - 06/06/15 10:19 AM (8 years, 7 months ago) |
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Quote:
Mad Season said: Rip stone in half, take sterile scalpel and cut out a meaty piece of it. A place that hadn't touched open air. Inside a still air box, as sterile as you can be. Drop the flesh on a petri dish of agar. I'd probably make a few dishes to be safe. Transfer when clean and if you get a clean culture, tiger drop it into grain jars.
Cloned stone LI or LC inoculation of grains intended for stone producer is better than dropping an agar chunk in. The jar will colonize evenly and rapidly and begin producing stones sooner. But since these jars often sit for very extended periods of time, I guess it doesn't matter a whole lot which inoculation method you use as long as it's clean. If you're wanting the fastest way to stones though, it's something to consider.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Juiceh]
#21769864 - 06/06/15 10:33 AM (8 years, 7 months ago) |
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Lc and li works for sure I was just being simplistic. I think any inoculant has its pros and cons. I've never done stones so it's cool to know liquid is better.
Still highly recommend cleaning it up on agar and testing the liquid on agar to make sure it's clean
Edited by Mad Season (06/06/15 11:45 AM)
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mromgwtf
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Mad Season]
#21770144 - 06/06/15 11:43 AM (8 years, 7 months ago) |
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Ok, I think I will go with the easiest route. Buy a sterilized grow bag of rye, and a sterilized syringe of spores. Inject that stuff and wait. It has 1400 grams of rye in it, how much truffles it'll yield you think?
Btw, here are the products I'd use: http://www.zamnesia.com/sporemate/2308-vial-psilocybe-tampanensis-pollock-.html http://www.zamnesia.com/mushroom-grow-supplies/2697-bag-sterilized-rye.html
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Edited by mromgwtf (06/06/15 11:44 AM)
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Dhearic


Registered: 01/24/14
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
#21770156 - 06/06/15 11:47 AM (8 years, 7 months ago) |
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A shit ton more than the 15 grams you originally bought. Don't forget to do your inoculation in a still air box.
I'd suggest you use the search function and read a bit about stone production and basic agar stuff. You don't want to have to keep buying spores right?
Pasty plates would probably be great for you
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Credit where credit is due.
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Aero
Orea


Registered: 11/01/13
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Dhearic]
#21770288 - 06/06/15 12:24 PM (8 years, 7 months ago) |
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As above. Break the stone in half in a glovebox. Get a sterile scalpel, cut a small piece out from the core and place it on agar. Should start to colonize in 2-3days Good luck
-------------------- SPREAD THE SPORES
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mromgwtf
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Aero]
#21770341 - 06/06/15 12:37 PM (8 years, 7 months ago) |
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Quote:
Aero said: As above. Break the stone in half in a glovebox. Get a sterile scalpel, cut a small piece out from the core and place it on agar. Should start to colonize in 2-3days Good luck 
What if I added hydrogen peroxide into the agar? As far as I know it'd kill all the mold spores and other containment, but not the mycelium, so I wouldn't need to worry that much about the sterility. Am I right?
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Aero
Orea


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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
#21770355 - 06/06/15 12:42 PM (8 years, 7 months ago) |
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Hydrogen peroxide breaks down pretty fast. Won't be there after sterilizing the agar. U can put antibiotics in the mix but the same applies it won't survive the heat only if u add it after. I prefer not to add it cause I like to see how clean the procedure was Dont try to take the easy way. If u work clean, u have contam free first plates. I never had contams while cloning..
-------------------- SPREAD THE SPORES
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
#21770367 - 06/06/15 12:45 PM (8 years, 7 months ago) |
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Lots of people recommend running through an antibacterial plate at least once to kill hidden contams. Personally I love sterile technique and improving my techniques, so I don't use antibacterial agar. It will work though.
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kushroom



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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
#21770664 - 06/06/15 02:19 PM (8 years, 7 months ago) |
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Quote:
mromgwtf said:
Quote:
Buck513 said:

You can clone a stone by taking a tissue sample from a stone. Plenty of people do that
So basically take a piece of the inside of the stone with a syringe or anything, and drop it in to a prepared jar of rice/ryegrass seed/whatever?
Lol, just look into agar man, read up on some teks than come back with questions, remember the search bar can be a best friend at times -kushroom
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 All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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