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Offlinemromgwtf
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Registered: 06/06/15
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Growing scletoria without spores? (Directly from an existing truffle)
    #21768723 - 06/06/15 02:30 AM (8 years, 7 months ago)

Hey. I've recently bought 15 grams of psilocybe atlantis. I've made a tea with it with tons of lemon juice. I took it together with my friend. It was our first time, it was quite amazing.

I left out a small single truffle for the purpose of cultivation. How would I go about growing it? I've read quite a lot about scletoria cultivation, but all of those techniques use spores. How can I grow it from an existing truffle?
I can't imagine how I'd put it inside of a jar that has been sterilized without contaminating it. Can somebody give me some pointers? Thanks in advance.


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
    #21768816 - 06/06/15 03:18 AM (8 years, 7 months ago)

A-G-A-R


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Fail to plan and you plan to fail.

Enter the Ban Lottery


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OfflineYaMoonSun
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Buck513]
    #21768848 - 06/06/15 03:33 AM (8 years, 7 months ago)



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Offlinemromgwtf
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Buck513]
    #21768903 - 06/06/15 04:14 AM (8 years, 7 months ago)

Quote:

YaMoonSun said:
This is a pretty good read; Good luck.
http://www.shroomery.org/forums/showflat.php/Number/20833312/fpart/1





So if I prepare a proper agar petri dish, and drop the stone in there, will it grow?


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Edited by mromgwtf (06/06/15 04:15 AM)


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OfflineMUSHmania
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
    #21768904 - 06/06/15 04:16 AM (8 years, 7 months ago)

Quote:

mromgwtf said:
Quote:

YaMoonSun said:
This is a pretty good read; Good luck.
http://www.shroomery.org/forums/showflat.php/Number/20833312/fpart/1





So if I prepare a proper agar petri dish, and drop the stone in there, will it grow?



Take a tissue sample and clone to agar


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Invisibleoontribe
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: MUSHmania]
    #21768919 - 06/06/15 04:33 AM (8 years, 7 months ago)

i think you take a tissue usually from the stem if they are fruits and you drop it in the agar and they start to grow.
dunno about stones!


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: oontribe]
    #21768992 - 06/06/15 05:13 AM (8 years, 7 months ago)

:lolwut:

You can clone a stone by taking a tissue sample from a stone. Plenty of people do that


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Offlinemromgwtf
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Buck513]
    #21769003 - 06/06/15 05:23 AM (8 years, 7 months ago)

Quote:

Buck513 said:
:lolwut:

You can clone a stone by taking a tissue sample from a stone. Plenty of people do that




So basically take a piece of the inside of the stone with a syringe or anything, and drop it in to a prepared jar of rice/ryegrass seed/whatever?


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
    #21769021 - 06/06/15 05:33 AM (8 years, 7 months ago)

No. Take a tissue sample and put it on AGAR.
Transfer a couple times to clean it up if need be. You should have a strictly stone producing culture.


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Invisibleoontribe
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Buck513]
    #21769052 - 06/06/15 05:58 AM (8 years, 7 months ago)

yea i think it work the same as fruits.


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InvisibleMad Season
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: oontribe]
    #21769400 - 06/06/15 08:31 AM (8 years, 7 months ago)

Rip stone in half, take sterile scalpel and cut out a meaty piece of it. A place that hadn't touched open air. Inside a still air box, as sterile as you can be. Drop the flesh on a petri dish of agar. I'd probably make a few dishes to be safe. Transfer when clean and if you get a clean culture, tiger drop it into grain jars.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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Edited by Mad Season (06/06/15 08:33 AM)


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Offlinemromgwtf
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Mad Season]
    #21769642 - 06/06/15 09:45 AM (8 years, 7 months ago)

What about growing it in a jar full of agar with nutrients? Agar seems so nice because you can see through it, and so you are fully aware of what's going on in there.


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InvisibleMad Season
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
    #21769691 - 06/06/15 09:55 AM (8 years, 7 months ago)

Yes. You can use wide mouth half pints like you do for pf tek, or pp5 containers.

http://www.shroomery.org/forums/showflat.php/Number/19208976


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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InvisibleJuiceh
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Mad Season]
    #21769798 - 06/06/15 10:19 AM (8 years, 7 months ago)

Quote:

Mad Season said:
Rip stone in half, take sterile scalpel and cut out a meaty piece of it. A place that hadn't touched open air. Inside a still air box, as sterile as you can be. Drop the flesh on a petri dish of agar. I'd probably make a few dishes to be safe. Transfer when clean and if you get a clean culture, tiger drop it into grain jars.



Cloned stone LI or LC inoculation of grains intended for stone producer is better than dropping an agar chunk in. The jar will colonize evenly and rapidly and begin producing stones sooner. But since these jars often sit for very extended periods of time, I guess it doesn't matter a whole lot which inoculation method you use as long as it's clean. If you're wanting the fastest way to stones though, it's something to consider.


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InvisibleMad Season
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Juiceh]
    #21769864 - 06/06/15 10:33 AM (8 years, 7 months ago)

Lc and li works for sure :smile: I was just being simplistic. I think any inoculant has its pros and cons. I've never done stones so it's cool to know liquid is better.

Still highly recommend cleaning it up on agar and testing the liquid on agar to make sure it's clean


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/06/15 11:45 AM)


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Offlinemromgwtf
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Mad Season]
    #21770144 - 06/06/15 11:43 AM (8 years, 7 months ago)

Ok, I think I will go with the easiest route. Buy a sterilized grow bag of rye, and a sterilized syringe of spores. Inject that stuff and wait. It has 1400 grams of rye in it, how much truffles it'll yield you think?

Btw, here are the products I'd use:
http://www.zamnesia.com/sporemate/2308-vial-psilocybe-tampanensis-pollock-.html
http://www.zamnesia.com/mushroom-grow-supplies/2697-bag-sterilized-rye.html


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Edited by mromgwtf (06/06/15 11:44 AM)


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OfflineDhearic
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
    #21770156 - 06/06/15 11:47 AM (8 years, 7 months ago)

A shit ton more than the 15 grams you originally bought. Don't forget to do your inoculation in a still air box.

I'd suggest you use the search function and read a bit about stone production and basic agar stuff. You don't want to have to keep buying spores right?

Pasty plates would probably be great for you :thumbup:


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OfflineAero
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Dhearic]
    #21770288 - 06/06/15 12:24 PM (8 years, 7 months ago)

As above.
Break the stone in half in a glovebox. Get a sterile scalpel, cut a small piece out from the core and place it on agar.
Should start to colonize in 2-3days
Good luck :wink:


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SPREAD THE SPORES



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Offlinemromgwtf
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: Aero]
    #21770341 - 06/06/15 12:37 PM (8 years, 7 months ago)

Quote:

Aero said:
As above.
Break the stone in half in a glovebox. Get a sterile scalpel, cut a small piece out from the core and place it on agar.
Should start to colonize in 2-3days
Good luck :wink:




What if I added hydrogen peroxide into the agar? As far as I know it'd kill all the mold spores and other containment, but not the mycelium, so I wouldn't need to worry that much about the sterility. Am I right?


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OfflineAero
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
    #21770355 - 06/06/15 12:42 PM (8 years, 7 months ago)

Hydrogen peroxide breaks down pretty fast. Won't be there after sterilizing the agar. U can put antibiotics in the mix but the same applies it won't survive the heat only if u add it after. I prefer not to add it cause I like to see how clean the procedure was
Dont try to take the easy way. If u work clean, u have contam free first plates. I never had contams while cloning..


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SPREAD THE SPORES



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InvisibleMad Season
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
    #21770367 - 06/06/15 12:45 PM (8 years, 7 months ago)

Lots of people recommend running through an antibacterial plate at least once to kill hidden contams. Personally I love sterile technique and improving my techniques, so I don't use antibacterial agar. It will work though.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinekushroom
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Re: Growing scletoria without spores? (Directly from an existing truffle) [Re: mromgwtf]
    #21770664 - 06/06/15 02:19 PM (8 years, 7 months ago)

Quote:

mromgwtf said:
Quote:

Buck513 said:
:lolwut:

You can clone a stone by taking a tissue sample from a stone. Plenty of people do that




So basically take a piece of the inside of the stone with a syringe or anything, and drop it in to a prepared jar of rice/ryegrass seed/whatever?




Lol, just look into agar man, read up on some teks than come back with questions, remember the search bar can be a best friend at times -kushroom


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:/



All submitted posts are by Someone Who Isn't Me (SWIM) -  and in any event are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.


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