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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21835159 - 06/21/15 01:38 AM (8 years, 7 months ago)

Visual metabolites on a substrate don't always mean contams. Depending on genetics and substrate specificty, secondary metabolites can be excreted due to reaching a natural or artificial boundary. They can also be excreted due stress to the mycelium network from unfavorable environmental conditions.


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 24 days
Re: First PF Tek Cultivation [Re: insanemike]
    #21835170 - 06/21/15 01:42 AM (8 years, 7 months ago)

Quote:

insanemike said:
Visual metabolites on a substrate don't always mean contams. Depending on genetics and substrate specificty, secondary metabolites can be excreted due to reaching a natural or artificial boundary. They can also be excreted due stress to the mycelium network from unfavorable environmental conditions.




That would make sense given that I made my jars way too moist, and my mixture wasn't airy enough.


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
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Re: First PF Tek Cultivation [Re: WindWisperer]
    #21844630 - 06/23/15 03:46 AM (8 years, 7 months ago)

I checked on them today, haven't watered for a day. I will let them go another day without water, and water tomorrow night. I noticed that the one that was pinning in vitro started doing some funky stuff. The single mushroom coming off of it got fuzzy since the last time I saw it. On another note I got my first actual normal pin! It has popped up as of today on one of the cakes. Thanks for the watering advice, I'm sure it will make a world of a difference! I'll post pictures tomorrow.


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OfflinefirstTIMER420
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Re: First PF Tek Cultivation [Re: WindWisperer]
    #21846270 - 06/23/15 02:31 PM (8 years, 7 months ago)

good links that helped me and countless other people:

-misted cake project(what properly misted cakes should look like)
http://www.shroomery.org/forums/showflat.php/Number/21595437

-what it should and shouldn't look like(contams and such)
http://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/1/vc/1


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 24 days
Re: First PF Tek Cultivation [Re: firstTIMER420]
    #21849911 - 06/24/15 10:59 AM (8 years, 7 months ago)

Small update with pics! Pins!!!!


Here's the fuzzy one that pinned in vitro I was talking about







A little bit of yellowing on the edges of this one









Edited by WindWisperer (06/24/15 11:02 AM)


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InvisibleMad Season
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Registered: 09/16/12
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Re: First PF Tek Cultivation [Re: WindWisperer]
    #21849942 - 06/24/15 11:06 AM (8 years, 7 months ago)

Looks beautiful! :rockon: that fuzzy in vitro guy, I'd let him chill for a few days at least. Don't need to harm your cakes for a slow pin/abort. I'd just pick it when you're picking your first harvest from the cake.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
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Re: First PF Tek Cultivation [Re: Mad Season]
    #21849953 - 06/24/15 11:08 AM (8 years, 7 months ago)

Quote:

Mad Season said:
I'd just pick it when you're picking your first harvest from the cake.



Or when it matures....which ever comes first....


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InvisibleMad Season
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Re: First PF Tek Cultivation [Re: PussyFart]
    #21849960 - 06/24/15 11:10 AM (8 years, 7 months ago)

:whathesaid: that too my man.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
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Re: First PF Tek Cultivation [Re: Mad Season]
    #21849961 - 06/24/15 11:11 AM (8 years, 7 months ago)

Thanks man! I'll just let him do his thing in that case. I felt the verm on top of one of the cakes at the time of taking this picture, it still felt damp. Should I let it dry out even more before misting, or should I mist it lightly before it gets dry to the touch? Right now they haven't been misted since Monday night.

[EDIT] Just trying not to botch it since I way over watered earlier in these cakes lives.


Edited by WindWisperer (06/24/15 11:12 AM)


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InvisibleMad Season
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Re: First PF Tek Cultivation [Re: WindWisperer]
    #21849978 - 06/24/15 11:14 AM (8 years, 7 months ago)

You don't want it to get dry to the touch. You want it really close to being wet without having any pooled up water 24/7. Also known as glistening wet 24/7. What you had was puddles on the top of the cakes before. I'd go ahead and give them a light mist, based off your pics. One has yellow staining on the corner from getting dry, so you're overdue for a mist anyway.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/24/15 11:16 AM)


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 24 days
Re: First PF Tek Cultivation [Re: Mad Season]
    #21849994 - 06/24/15 11:18 AM (8 years, 7 months ago)

Quote:

Mad Season said:
You don't want it to get dry to the touch. You want it really close to being wet without having any pooled up water 24/7. Also known as glistening wet 24/7. What you had was puddles on the top of the cakes before. I'd go ahead and give them a light mist, based off your pics. One has yellow staining on the corner from getting dry, so you're overdue for a mist anyway.





Okay thanks for the misting advice. I had no idea what the yellowing was, thanks for clearing that up as well!


Edited by WindWisperer (06/24/15 11:18 AM)


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OfflinefirstTIMER420
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Re: First PF Tek Cultivation [Re: WindWisperer]
    #21850005 - 06/24/15 11:21 AM (8 years, 7 months ago)

I thought the yellowing was bacteria, shows what I know! :rofl:


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
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Re: First PF Tek Cultivation [Re: firstTIMER420]
    #21850042 - 06/24/15 11:29 AM (8 years, 7 months ago)

On another note I have decided to put my big boy pants on and do a Bulk grow in the near future. I have the stuff to do Pasty Plates, and I will be following Franks' Bulk grow method with Coir/WBS. I am just waiting on that precious 23qt Pressure Cooker!



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OfflinefirstTIMER420
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Re: First PF Tek Cultivation [Re: WindWisperer]
    #21852705 - 06/24/15 09:27 PM (8 years, 7 months ago)

franks 12 step?  exactly what im making the step to. gonna be fun! im just gonna wait on the pasty plates for now, have a feeling the timing isn't rigt since I just started a new job and cannot give them the full attention they deserve.

what brand of jars are those?


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
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Re: First PF Tek Cultivation [Re: firstTIMER420]
    #21852723 - 06/24/15 09:32 PM (8 years, 7 months ago)

You're silly. Agar requires very little attention. A lot less than tubs or jars imo. Very easy to manage too


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Edited by Mad Season (06/24/15 09:32 PM)


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
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Re: First PF Tek Cultivation [Re: firstTIMER420]
    #21852898 - 06/24/15 10:25 PM (8 years, 7 months ago)

Quote:

firstTIMER420 said:
franks 12 step?  exactly what im making the step to. gonna be fun! im just gonna wait on the pasty plates for now, have a feeling the timing isn't rigt since I just started a new job and cannot give them the full attention they deserve.

what brand of jars are those?




Ball, but I got the plastic lids because they are cheap, easy to punch big holes in, and I still have the metal lids if I want to do something else with em. Yeah I'd be lying if I said I wasn't a little nervous about agar, I need to step up my sterilization game by a LOT for when I start doing transfers, and colonizing jars with agar wedges. A SAB seems to be a must when you are doing open air transfers like that.


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InvisibleMad Season
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Re: First PF Tek Cultivation [Re: WindWisperer]
    #21852958 - 06/24/15 10:44 PM (8 years, 7 months ago)

It is necessary to have a SAB. I'd recommend 100+ qt clear tub. This is mine, literally took the melted 6" holes from the coffee can and taped them to the tub to try to make a still environment. I do all my work in this, bare armed with disposable latex gloves on my kitchen table or living room. Key is never to have your arm or hand hover over open media:
I made the holes at shoulder length and made the right one higher, since I normally have it above the media.

No need to be scared :smile:. Just need a good scalpel/#11 x-acto. I use a butane torch to heat OUTSIDE the box too. You also can breathe normally too. Don't need to get over the top and look away. It's actually pretty comfortable.

I like this video. Takes 5 minutes to make :smile:


Edited by Mad Season (06/24/15 11:30 PM)


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OfflinefirstTIMER420
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Re: First PF Tek Cultivation [Re: Mad Season]
    #21857016 - 06/25/15 08:31 PM (8 years, 7 months ago)

I agree, they are easy to make, that is, if you have a hole saw! The key is shoulder length apart to make sure your comfortable.  I used my 4 inch hole saw and it was done in like 2 minutes.  Also, I recommend placing it upside down on the table, with a fresh towel where the top is touching the table, to make sure there are no drafts coming in from the bottom.(lid off)

I followed this exactly every time I do it, and have had 0 contams(out of 12 jars, 6 each time)
be

if you follow that and RR's notes on his vids when inoculating youll be 100% fine.

and ill tell you, I was nervous my first time, but it also made me pay attention more.  People say the key with SAB is minimize your movements, think about what your going to do before you do it.

and I also have a 100qt tub(like 106 or something I forget)

Good luck!



Also, what tek did you follow to make your lids for those quart jars? im doing a monotub yet and I haven't decided on a lid design, but I think I wanna do polyfill stuffed like yours. how big is the hole(what bit used)


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12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
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Edited by firstTIMER420 (06/25/15 08:33 PM)


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InvisibleMad Season
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Registered: 09/16/12
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Re: First PF Tek Cultivation [Re: firstTIMER420]
    #21857053 - 06/25/15 08:38 PM (8 years, 7 months ago)

Hole saw works really well and is the best way to do it. Check out my video I posted for an alternative if you don't have a hole saw :smile: cause I just used the bottom of a coffee can, and melted my arm holes. I love melting my plastics apparently. That's how I made my monotub holes too. Took a 1" socket, torched it til red hot, and melted my mono holes.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 24 days
Re: First PF Tek Cultivation [Re: Mad Season]
    #21857975 - 06/25/15 11:37 PM (8 years, 7 months ago)

Thanks for the advice, I'm sure I have a few old 'nuts n bolts' hardware tins around. If not I have a shoddy soldering iron I don't care about anymore, since I have a hakko now. I will look for the coffee can first though since it will make a cleaner hole I think! The biggest hole saw I've ever used is a 2 inch, so LOL that's a big step! Expensive too.


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