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OfflineWindWisperer

Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
First PF Tek Cultivation
    #21768678 - 06/06/15 02:10 AM (8 years, 7 months ago)

[EDIT] Here is my journal where I moved updated stuff:
WBS / PF Tek Journal

Here are a few of my 12 jars that are 13 days along. Awesome rhizo. structure I'd say! Very excited to see where it goes from here.

Genes: Maza.
Method: Spore Syringe

Recipe used: 2 parts verm, 1 part BRF, water to field capacity

I was concerned I may have added too much water, my verm was on the finer side, and ended up holding more water than the coarse stuff.
I sterilized in a pot with rocks on top, and lids on the bottom to space the jars away from the heat source. I sterilized for 90 minutes at a calm boil/simmer. When I took them out there was a bit of water resting on the inside of the lid, which made me concerned that the dry verm layer might have gotten moist. So far all seems to be turning out very well despite these small things!

They have been inoculating in a tub in tub chamber that stays at a consistent 75-76 degrees F. 50% humidity on average, with just a blanket over the top to allow for air exchange. Would you suggest just leaving them out in the open in 70-72 degrees ambient temps?






[EDIT 07/16]
This is my second grow, including 6 KSSS, and 6 Chitwan. I am also undertaking some Agar work from a 10g Maz fruit that performed well.

-6 Days after Noc- (KSSS)


-9 Days after Noc- (KSSS)


-12 Days after Noc- (KSSS)


--Some Maz Agar Transfers--

This is transfer one after growing out for a few days! Great rhizomorphic growth, and fast to boot!





--

I will eventually just start posting in a journal unless I have questions if anybody is interested in watching this stuff pan out!




Edited by WindWisperer (07/27/15 01:54 AM)


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21768747 - 06/06/15 02:42 AM (8 years, 7 months ago)

Make sure to add the water to the verm and get that to field capacity and then add the brf. Water inside the jar lid? Did you put aluminum foil around the lids before sterilizing? Leaving them out on a shelf in room temp is preferred and light is beneficial at all stages of growth.


Edited by insanemike (06/06/15 02:44 AM)


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OfflineWindWisperer

Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
Re: First PF Tek Cultivation [Re: insanemike]
    #21768754 - 06/06/15 02:47 AM (8 years, 7 months ago)

Quote:

insanemike said:
Make sure to add the water to the verm and get that to field capacity and then add the brf. Water inside the jar lid? Did you put aluminum foil around the lids before sterilizing? Leaving them out on a shelf in room temp is preferred and light is beneficial at all stages of growth.




I meant a very small amount of water around a few of the inoculation points on maybe 4 of the jars had accumulated on the top of the lid. Yes I used foil when I boiled, but this only seemed to affect a few points on 4 jars. I used the recipe, then added the BRF, and it seemed much too dry so I added a tad more water until I got the few droplets when squeezed.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21768768 - 06/06/15 02:54 AM (8 years, 7 months ago)

I would always lean more toward the dry side. Too much moisture can cause more problems then if it were a little on the dry side. Besides, you add more moisture when noc'ing them up anyway.


Edited by insanemike (06/06/15 02:54 AM)


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OfflineWindWisperer

Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21768772 - 06/06/15 02:54 AM (8 years, 7 months ago)

Oh and of course, the tek followed to a T. I did however not use a SAB, I instead opted for a sealed off bathroom cleaned with bleach, and sprayed with disinfectant before entering. Masks, gloves, and a torch for sterilizing the needle between each jar was practiced. Thanks for the advise, I'll take them out of the TIT chamber tomorrow when I do the 4 day check. I'll take more pictures of said jars in the former pictures for reference of growth as well!

What recipe do you follow that seems to work? I used a natural VERM that seemed much finer than the Home Depot stuff people seem to be using around here. I a investing in a PC soon, I'm looking into either a 12qt, or a 16-23 qt if I can find one reasonably priced. Hopefully that will make the process a little simpler!


Edited by WindWisperer (06/06/15 02:56 AM)


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21768787 - 06/06/15 03:00 AM (8 years, 7 months ago)

No need for a sab when injecting into an injection port. Although I would if I were you plan on building one and get use to using it, for you will need it for future endeavors. Great job so far, the jars look good.

:vibin:


Edited by insanemike (06/06/15 03:00 AM)


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OfflineElement1
Stranger
Registered: 04/08/13
Posts: 57
Last seen: 7 years, 10 days
Re: First PF Tek Cultivation [Re: insanemike]
    #21769131 - 06/06/15 06:44 AM (8 years, 7 months ago)

Looks good keep us updated!


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OfflineWindWisperer

Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
Re: First PF Tek Cultivation [Re: Element1]
    #21772770 - 06/07/15 03:31 AM (8 years, 7 months ago)

Bump with small update! They came along along well the past 4 days, all of them doubled in area covered by myc! Still not quite there yet from the looks of it. Do some cakes end up showing some substrate still, or should I expect it to be 100% white when it is finished?



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Invisiblemushmagic
supporting radical habits
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Registered: 03/21/12
Posts: 5,372
Loc: Candyland
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21772791 - 06/07/15 03:49 AM (8 years, 7 months ago)

Cakes are usually completely white while bulk subs like coir may be a little thinner at parts making the sub a bit more visible through the myc IME.


--------------------


Trade list in journal (partially under construction; more to be added)


Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21772794 - 06/07/15 03:50 AM (8 years, 7 months ago)

Everything should be white but it can look a bit translucent with some grain or verm showing through. You need to let them consolidate for atleast a week after full colonization which should give the myc enough time to thicken up.


Edited by insanemike (06/07/15 03:51 AM)


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OfflineWindWisperer

Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
Re: First PF Tek Cultivation [Re: insanemike]
    #21772802 - 06/07/15 03:55 AM (8 years, 7 months ago)

Quote:

insanemike said:
Everything should be white but it can look a bit translucent with some grain or verm showing through. You need to let them consolidate for atleast a week after full colonization which should give the myc enough time to thicken up.




Okay so full colonization is described as the whole jar being lightly covered to fully covered by myc, and consolidating it is just allowing it to fill in? The clearer pic I posted, given that the whole jar is covered that way; how far along would you say it is? These are roughly 16 days along now.


Edited by WindWisperer (06/07/15 03:59 AM)


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21772840 - 06/07/15 04:41 AM (8 years, 7 months ago)

Well the pic on the right looks like it's ready for that week of consolidation but the pic on the left is tough to judge because of the lighting.


Edited by insanemike (06/07/15 04:41 AM)


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: First PF Tek Cultivation [Re: insanemike]
    #21775343 - 06/07/15 06:18 PM (8 years, 7 months ago)

You can probably start consolidation on the left as well :smile:


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
Re: First PF Tek Cultivation [Re: Mad Season]
    #21781480 - 06/09/15 01:16 AM (8 years, 7 months ago)

Here's a small update with 2 pics. I separated out 4 jars because they have a bit of yellowish to them. I assume this is only metabolites, but I've been keeping an eye on them. The other jars are filling in quite nicely!

Let me know if this is not metabolites, and they should be chucked! They have no bad smells coming from them (I smelled closely around the noc points.)





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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21781513 - 06/09/15 01:39 AM (8 years, 7 months ago)

I'm concerned about that creamy growth over top of that healthy looking myc.


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
Re: First PF Tek Cultivation [Re: insanemike]
    #21799752 - 06/12/15 09:43 PM (8 years, 7 months ago)

About to birth 6 jars that are for sure done, I will update with pictures tomorrow. Given the colonization time etc should I let them dunk for 12 or 24 hours? Also I cannot keep the water lower than 50 degrees F. for longer than a few hours, should I put them in the fridge?

Also I realized I made a critical error, the water I used was in fact distilled. I am surprised I haven't had issues, they will be put in tap water for the dunk.


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OfflineTravelAgency
The ongoing "wow"

Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21799890 - 06/12/15 10:16 PM (8 years, 7 months ago)

Looks super healthy mate. Those runners! Should fruit nicely after you let them consolidate.


--------------------





Indoor Greenhouse Build 
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OfflineShroominMe
Stranger
Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21800001 - 06/12/15 10:50 PM (8 years, 7 months ago)

Quote:

WindWisperer said:
About to birth 6 jars that are for sure done, I will update with pictures tomorrow. Given the colonization time etc should I let them dunk for 12 or 24 hours? Also I cannot keep the water lower than 50 degrees F. for longer than a few hours, should I put them in the fridge?



I'd go 12 hours but others will say 24.  Your call :wink:
Yes, put them in the fridge

Quote:

WindWisperer said:
Also I realized I made a critical error, the water I used was in fact distilled. I am surprised I haven't had issues, they will be put in tap water for the dunk.



Huh?  Distilled water IMO is definitely preferable to tap water which has chlorine and fluorine added.


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OfflineWindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
Re: First PF Tek Cultivation [Re: ShroominMe]
    #21800033 - 06/12/15 10:58 PM (8 years, 7 months ago)

I had read somewhere not to use distilled water, oh well I'll just use carbon purified tap water next time so there are no hard deposits. They will have been consolidating for a week as of tomorrow! I'm very excited, and only one jar has lagged behind really badly from the rest. 6 jars are for sure 100% ready to fruit. I'll do the other 6 in a few days since they were about 30% behind the others. I'm going to poke the holes in my sgfc tomorrow, and lay down the perlite. About 5 inches of perlite is optimal aye?


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OfflineShroominMe
Stranger
Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: First PF Tek Cultivation [Re: WindWisperer]
    #21800463 - 06/13/15 01:41 AM (8 years, 7 months ago)

The only reason to not use distilled water is because of the benefits of additional minerals in spring water.  I suspect all tap and bottled water, but that's just me :laugh:

I've heard 4" but of course the only important point is to raise your humidity level to the correct level.  Depends on your setup.

You're on the final stretch!  Yessss!!!! :thumbup::thumbup::thumbup:


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