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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
Re: Low Yield, Strange Growth, Noob Questions [Re: bodhisatta]
    #21773614 - 06/07/15 11:00 AM (8 years, 7 months ago)

I knew what you meant Bod, and so did uhh everyone else?


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InvisibleLeopardMan
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Registered: 09/21/09
Posts: 5,463
Loc: A tree house
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Re: Low Yield, Strange Growth, Noob Questions [Re: bodhisatta]
    #21774110 - 06/07/15 01:35 PM (8 years, 7 months ago)

Quote:

bodhisatta said:
Don't quote out of context the very next sentence. OMC defines sterile very different than a microbiology textbook.

I have a few posts on the finer details of pasteurization (and PUs), sanitization, and sterilization and what it really means for us in terms of log reduction value.

If a jar gets to 100% OMC refers to the jar as having been sufficiently sterile as to get you mycelium to 100% sure you could have another mold or bacteria in there but we're assuming the cakes are free of contamination. Thus
"birth the cakes are very contamination resistant."

They've already been contaminated with cubensis rot. They're not sterile by definition after inoculation that's obvious. OMC makes distinctions to the lexicon of biology terms to jam them into working with amateur boomer cultivation

semantic vs scientific definitions, they're what makes this place work a little better in practice.




Sterile means sterile. A jar at full colonization is not sterile, There is a mycelium and there are bacteria and possibly mold spores as well. It's just wrong man, no need to argue that black is white and bring some friends to help you.
Also, care to explain what "log reduction value" is? I need to update my dictionary.


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-


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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Low Yield, Strange Growth, Noob Questions [Re: LeopardMan]
    #21774282 - 06/07/15 02:14 PM (8 years, 7 months ago)

Autoclave software aims for SAL sterility assurance level. If you say have a load for your autoclave and its harboring 1mil CFU before sterilization. Then you need a 6 log reduction to theoretically 0 CFU survived. That's not sterile though. You sterilize to an additional six log to achieve 10^-6 chance of one CFU in each media in your autoclave load. We don't know our original contaminant load but more than 12 total log reduction in CFU is at minimum the aim of sterilization for mycological purpose.


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
Re: Low Yield, Strange Growth, Noob Questions [Re: bodhisatta]
    #21774694 - 06/07/15 03:43 PM (8 years, 7 months ago)

Everyone on these boards say they are "sterilizing" their jars, it's the lingo we use.  We know they aren't truly sterile.  To suggest that Bod didn't know what the dictionary definition of sterile means is ridiculous.  It's you who are out of step with the way people talk around here.  We also say a quart of grain spawn when we usually mean less than a quart.  We also say trich, when we see green mold, even though it could be another type.  If you don't like the way we use the english language around here well start a petition or something to change it, but going after Bod about our vernacular is lame.

I'm not biased toward or against Bod by the way, I butt heads with him almost daily it seems.  When I see something way out of line I tend to correct it, it's that simple.


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InvisibleLeopardMan
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Registered: 09/21/09
Posts: 5,463
Loc: A tree house
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Re: Low Yield, Strange Growth, Noob Questions [Re: invitro]
    #21774713 - 06/07/15 03:47 PM (8 years, 7 months ago)

Quote:

invitro said:
Everyone on these boards say they are "sterilizing" their jars, it's the lingo we use.  We know they aren't truly sterile.  To suggest that Bod didn't know what the dictionary definition of sterile means is ridiculous.  It's you who are out of step with the way people talk around here.  We also say a quart of grain spawn when we usually mean less than a quart.  We also say trich, when we see green mold, even though it could be another type.  If you don't like the way we use the english language around here well start a petition or something to change it, but going after Bod about our vernacular is lame.

I'm not biased toward or against Bod by the way, I butt heads with him almost daily it seems.  When I see something way out of line I tend to correct it, it's that simple.




I have been around since 2009 and sterile always meant sterile, sorry :shrug: Anyway I have nothing against you or Bodhisatta :peace:


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
Re: Low Yield, Strange Growth, Noob Questions [Re: LeopardMan]
    #21774720 - 06/07/15 03:48 PM (8 years, 7 months ago)

I've been around long enough to know you are way wrong, nobody's jars are sterile even after hours in the pressure cooker, or did you not know this?


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