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Leon76


Registered: 05/18/13
Posts: 78
Last seen: 7 months, 27 days
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Can mushrooms be positively identified through their mycelium?
#21763987 - 06/04/15 11:36 PM (8 years, 7 months ago) |
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Can mushrooms be positively identified through their mycelium?
-------------------- “I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.” ― Edgar Allan Poe
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
#21768192 - 06/05/15 11:23 PM (8 years, 7 months ago) |
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If you take a piece of myc and DNA sequence it, yes. Otherwise no.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Leon76


Registered: 05/18/13
Posts: 78
Last seen: 7 months, 27 days
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Re: Can mushrooms be positively identified through their mycelium? [Re: 36fuckin5]
#21768744 - 06/06/15 02:41 AM (8 years, 7 months ago) |
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Wouldn't happen to have a ballpark cost range, would you?
-------------------- “I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.” ― Edgar Allan Poe
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
#21769223 - 06/06/15 07:35 AM (8 years, 7 months ago) |
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Somewhere between very expensive- unaffordable.
Why not just fruit the mycelium in question?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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drake89
Mushroom Magnate



Registered: 06/26/11
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Re: Can mushrooms be positively identified through their mycelium? [Re: maddchef]
#21769927 - 06/06/15 10:49 AM (8 years, 7 months ago) |
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this day in age it's really not that expensive, provided you can do the extraction and amplification yourself. Maybe $50. $250 for someone to do all the work for you
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matsc
Stranger



Registered: 09/17/12
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Re: Can mushrooms be positively identified through their mycelium? [Re: drake89]
#21771686 - 06/06/15 07:50 PM (8 years, 7 months ago) |
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It costs my lab about 40$ to run a sequence (4 reactions), less if we're running a bunch at once. DNA extraction is a bit more involved, and whether you send off PCR product or bulk DNA makes a difference as well.
That said there are a few, very very few, fungi you can ballpark the ID of from mycelial features. But even that requires a lot of practice and some higher end optical equipment. And even then its usually only to the genus level.
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drake89
Mushroom Magnate



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Re: Can mushrooms be positively identified through their mycelium? [Re: matsc]
#21773085 - 06/07/15 07:50 AM (8 years, 7 months ago) |
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Quote:
matsc said: It costs my lab about 40$ to run a sequence (4 reactions), less if we're running a bunch at once. DNA extraction is a bit more involved, and whether you send off PCR product or bulk DNA makes a difference as well.
That said there are a few, very very few, fungi you can ballpark the ID of from mycelial features. But even that requires a lot of practice and some higher end optical equipment. And even then its usually only to the genus level.
if it's pink, purple, or orange I can pretty well tell ya what it is
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matsc
Stranger



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Re: Can mushrooms be positively identified through their mycelium? [Re: drake89]
#21773636 - 06/07/15 11:08 AM (8 years, 7 months ago) |
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Orange = Neurospora crassa! Or maybe Cryphonectria parasitica. Those are my guesses ^_^
But I can ID a few nematophagus fungi from their hyphal traps. And Phymatotrichopsis omnivorum has those characteristic cruiciform branches that pop out pretty well...
And... um.... thats about all I can think of off the top of my head without getting into spore morphological nonsense. Much easier to spend a few hours doing an ITS run and handing it off to the sequencing lab .
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Leon76


Registered: 05/18/13
Posts: 78
Last seen: 7 months, 27 days
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Re: Can mushrooms be positively identified through their mycelium? [Re: matsc]
#21775976 - 06/07/15 08:42 PM (8 years, 7 months ago) |
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Police found a few mycelium jars of mine. No shrooms or anything, just jars and some spore syringes. I am wondering if I deny that they are cubensis will they have them dna sequenced? Would they in turn have to dna sequence every jar they found? How cost effective is this going to be for them? My plan is to deny, for the present, that they are indeed cubensis. It seems most everyone around here, including the police and attorneys, dont know shit about this. By the questions the detective was asking me I could tell he was pretty clueless about this stuff. He actually asked me if I would be willing to teach him more about the process and he would help me out in return. Naturally I told him top go f himself but since I have come to realize the police are ignorant I am wondering if I should tell them they are legal edible mushrooms.
P.S. They found eight jars
-------------------- “I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.” ― Edgar Allan Poe
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
#21776028 - 06/07/15 08:53 PM (8 years, 7 months ago) |
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Don't tell them anything at all. Talk to your lawyer and only your lawyer. They'll probably send at least a sample off.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Leon76


Registered: 05/18/13
Posts: 78
Last seen: 7 months, 27 days
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Re: Can mushrooms be positively identified through their mycelium? [Re: 36fuckin5]
#21776090 - 06/07/15 09:06 PM (8 years, 7 months ago) |
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Yes, I assume they will send a sample off. My question is can it be identified using a reasonable cost procedure. I cant see them spending thousands of dollars to dna sequence some mushroom jars. I have been charged with manufacturing but even the lead detective told me he knew i wasnt selling anything as they didnt see ANY traffic coming and going and I have never sold a single thing. I am strictly a consumer. Apparently they staked out my house for 6 weeks or so thinking I was growing massive amounts of pot. A neighbor saw all my Reaper and Bhutlah peppers in the window that I had started indoors for the summer planting season and called the police and told them I was growing smoke. They had a warrant for marijuana and didnt find anything worth charging me but while destroying my house found the shroom products and charged me. My lawyer is sure that I can get this down to a simple possession charge, if not dismissed entirely, as they didnt find much at all. So I am wondering if it is worth their time and cost for this all to come down to a simple possession charge.
-------------------- “I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.” ― Edgar Allan Poe
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matsc
Stranger



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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
#21776120 - 06/07/15 09:14 PM (8 years, 7 months ago) |
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I once had the cops sicced on my apartment because of my chili plants. Someone saw my grow light through the window and called it in. Very annoying.
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Leon76


Registered: 05/18/13
Posts: 78
Last seen: 7 months, 27 days
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Re: Can mushrooms be positively identified through their mycelium? [Re: matsc]
#21776383 - 06/07/15 10:16 PM (8 years, 7 months ago) |
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Thats exactly what happened to me. I would take them out in the morning when the sun was up but here in the Midwest we have late frosts so I would carry them in at night, put them on a dresser directly in front of a window facing the street, and light them up with a couple cfl's until the morning and then do it all over again. Apparently thats suspicious behavior and enough to get a search warrant. I have about 40 pepper plants so I guess they thought i was big time. Man, that pig was so mad when he realized I didnt have a grow operation in my house. If I wasnt so pissed and utterly shocked I would have laughed my ass off. Fucking PIGS!!!!!!!!!!!!!!!!!
-------------------- “I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.” ― Edgar Allan Poe
Edited by Leon76 (06/07/15 10:19 PM)
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Jenn
Supreme Leader



Registered: 04/27/15
Posts: 200
Last seen: 7 years, 11 months
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Re: Can mushrooms be positively identified through their mycelium? *DELETED* [Re: Leon76]
#21779232 - 06/08/15 03:11 PM (8 years, 7 months ago) |
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Post deleted by JennReason for deletion: I don't like it anymore.
-------------------- Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.
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Jenn
Supreme Leader



Registered: 04/27/15
Posts: 200
Last seen: 7 years, 11 months
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Re: Can mushrooms be positively identified through their mycelium? [Re: Jenn]
#21783717 - 06/09/15 03:19 PM (8 years, 7 months ago) |
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-------------------- Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.
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Jenn
Supreme Leader



Registered: 04/27/15
Posts: 200
Last seen: 7 years, 11 months
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Re: Can mushrooms be positively identified through their mycelium? [Re: Jenn]
#21798303 - 06/12/15 04:20 PM (8 years, 7 months ago) |
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Update?
-------------------- Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.
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nooneman


Registered: 04/24/09
Posts: 14,561
Loc: Utah
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Re: Can mushrooms be positively identified through their mycelium? [Re: Jenn]
#21798398 - 06/12/15 04:34 PM (8 years, 7 months ago) |
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Preview of coming attractions: OP will be charged with manufacture, distribution and possession of a controlled substance. They won't have it identified by a mycologist. Instead, they'll send it over to a chem lab, and it'll test positive for psilocybin.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Can mushrooms be positively identified through their mycelium? [Re: nooneman]
#21798540 - 06/12/15 05:00 PM (8 years, 7 months ago) |
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Waait. So they staked out your house for 6 weeks and saw no evidence of drug activities but somehow got a warrant to search your house? Seriously wtf.
Something is missing from this story
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
Edited by maddchef (06/12/15 05:00 PM)
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Jenn
Supreme Leader



Registered: 04/27/15
Posts: 200
Last seen: 7 years, 11 months
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Re: Can mushrooms be positively identified through their mycelium? [Re: maddchef]
#21802074 - 06/13/15 11:02 AM (8 years, 7 months ago) |
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Well, hopefully Op comes back and hooks us up with the dets... I love shit like this!
-------------------- Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.
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Leon76


Registered: 05/18/13
Posts: 78
Last seen: 7 months, 27 days
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Re: Can mushrooms be positively identified through their mycelium? [Re: Jenn]
#22124891 - 08/21/15 10:43 PM (8 years, 5 months ago) |
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Well, heres an update for those that asked. My 14 year old son, along with his mother, my ex-girlfriend, turned me in. My son broke into my locked grow room one night, took pictures, gave them to his mother and his mother called the police. They apparently did a trash search and found some discarded leaves from the pot and that was enough for the warrant. I have been charged with manufacturing a controlled substance but am getting accepted into drug court so no jail time. But, they COULD NOT test the jars of mycelium or the liquid cultures I had. I was originally charged with manufacturing marijuana as well as manufacturing mushrooms but as the lab was not able to test the jars, and they tried, the manufacturing of mushrooms charge was dismissed leaving only the marijuana charge. My own fucking son. I am still in shock about that. But again, they could not test the mycelium or the LQ's so they kicked that charge. Thought that would be pretty interesting for some of you. Not sure why they couldn't, just know the D.A. said they were unable. So I have to be a good boy for the next 12 to 18 months and not dabble in anything, which really sucks ass, but it is much better than the penitentiary.
-------------------- “I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.” ― Edgar Allan Poe
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
#22125039 - 08/21/15 11:27 PM (8 years, 5 months ago) |
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That really fucking sucks, and I feel for you. Nobody should go to jail for reproducing nature.
That being said, rule #1 is tell nobody. And that means people that used to live in your balls or people that you inseminated. I hate that you have to deal with this, but I hope anyone else reading this will take it to heart and realize that you can only trust you with your safety. Maybe your story can keep a dozen anons from going to jail.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Leon76


Registered: 05/18/13
Posts: 78
Last seen: 7 months, 27 days
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Re: Can mushrooms be positively identified through their mycelium? [Re: 36fuckin5]
#22125143 - 08/21/15 11:59 PM (8 years, 5 months ago) |
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I didn't tell them. I guess my son could smell it through the room and when he told his mother I am sure she convinced him to take the pics and because she is a spiteful bitch she called the police. There was a tiny smell from the grow room but I never imagined in a million years that my son would ever do something like this to his own father. I also have three small kids ages 3, 2, and 8 months, I got married about 6 years ago, that were placed in a foster home, someone they knew thank God, because the lead detective said that mushroom spores produce very toxic spores that spread through the house and that was endangering their lives. And now they can't even prove that they were illegal. We wife got our kids back about a month ago but I have had to move out of the house, I took full responsibility for the whole thing, and for the time being am living in a 300 square foot apartment until DHHS says I can be trusted to be back in the home. My wife is a nurse and has never done a drug or even had a speeding ticket in her whole life but they took her to jail and plastered us all over the local news only to drop all the charges against her. Absolute nightmare, for 4 plants. That's all I had. Just enough for me to not have to buy anything from anybody. Stuffs getting so expensive these days. Never sold a single gram of it to anyone. But you are correct in saying you never know who will snitch you out. But God damn, my own fucking son.
-------------------- “I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.” ― Edgar Allan Poe
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
#22125286 - 08/22/15 01:02 AM (8 years, 5 months ago) |
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There would be no point in identifying the species because the species aren't illegal. They would test for psilocybin/psilocin and colonizing jars and LCs generally contain none.
Quote:
the lead detective said that mushroom spores produce very toxic spores that spread through the house and that was endangering their lives.
Don't you just love when police make shit up and pretend they know what they're talking about.
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Leon76


Registered: 05/18/13
Posts: 78
Last seen: 7 months, 27 days
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Re: Can mushrooms be positively identified through their mycelium? [Re: Kizzle]
#22126804 - 08/22/15 01:08 PM (8 years, 5 months ago) |
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I think they did test them for Psilocybin. I believe they just came negative, which is interesting as I have read on here several times that colonized jars contain this chemical, as well as some techs that claim to be able to extract Psilocybin from colonized jars. There is no way they did not attempt to test a sample. It just had to come back negative.
-------------------- “I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.” ― Edgar Allan Poe
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
#22129529 - 08/23/15 03:16 AM (8 years, 5 months ago) |
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It's possible to extract psilocynin from the mycelium but the mycelium doesn't start producing it until pinning begins.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: Can mushrooms be positively identified through their mycelium? [Re: Kizzle]
#22130581 - 08/23/15 11:53 AM (8 years, 5 months ago) |
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Kizzles right, this happened with PF.
The mycelium is legal until it is a container of psilocybin or if the conspiracy to attempt to manufacture psilocybin from the mycelium is made. If using a large enough potassium deficiency or extending the trophophase to prevent secondary metabolites i believe the mycelium of cubensis could be handled legally 
If they are mature you are done for, if they are young you are technically within your legal limits as a hobbyist mycologist.
This is why scientists and not politicians should be in charge of public health law.
As not your lawyer i advise you to stick to gourmet/medicinal species and going hunting or to a phish concert for your specialized needs
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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