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OfflineLeon76
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Registered: 05/18/13
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Can mushrooms be positively identified through their mycelium?
    #21763987 - 06/04/15 11:36 PM (8 years, 7 months ago)

Can mushrooms be positively identified through their mycelium?


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“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe


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Invisible36fuckin5
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
    #21768192 - 06/05/15 11:23 PM (8 years, 7 months ago)

If you take a piece of myc and DNA sequence it, yes. Otherwise no.


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Redd Foxx said:
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Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

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i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineLeon76
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Re: Can mushrooms be positively identified through their mycelium? [Re: 36fuckin5]
    #21768744 - 06/06/15 02:41 AM (8 years, 7 months ago)

Wouldn't happen to have a ballpark cost range, would you?


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“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe


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Invisiblemaddchef
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
    #21769223 - 06/06/15 07:35 AM (8 years, 7 months ago)

Somewhere between very expensive- unaffordable.

Why not just fruit the mycelium in question?


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In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

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:havesomescience:


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Offlinedrake89
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Re: Can mushrooms be positively identified through their mycelium? [Re: maddchef]
    #21769927 - 06/06/15 10:49 AM (8 years, 7 months ago)

this day in age it's really not that expensive, provided you can do the extraction and amplification yourself.  Maybe $50.  $250 for someone to do all the work for you :wink:


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Offlinematsc
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Re: Can mushrooms be positively identified through their mycelium? [Re: drake89]
    #21771686 - 06/06/15 07:50 PM (8 years, 7 months ago)

It costs my lab about 40$ to run a sequence (4 reactions), less if we're running a bunch at once. DNA extraction is a bit more involved, and whether you send off PCR product or bulk DNA makes a difference as well.

That said there are a few, very very few, fungi you can ballpark the ID of from mycelial features. But even that requires a lot of practice and some higher end optical equipment. And even then its usually only to the genus level.


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Offlinedrake89
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Re: Can mushrooms be positively identified through their mycelium? [Re: matsc]
    #21773085 - 06/07/15 07:50 AM (8 years, 7 months ago)

Quote:

matsc said:
It costs my lab about 40$ to run a sequence (4 reactions), less if we're running a bunch at once. DNA extraction is a bit more involved, and whether you send off PCR product or bulk DNA makes a difference as well.

That said there are a few, very very few, fungi you can ballpark the ID of from mycelial features. But even that requires a lot of practice and some higher end optical equipment. And even then its usually only to the genus level.




if it's pink, purple, or orange I can pretty well tell ya what it is :wink:


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Offlinematsc
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Re: Can mushrooms be positively identified through their mycelium? [Re: drake89]
    #21773636 - 06/07/15 11:08 AM (8 years, 7 months ago)

Orange = Neurospora crassa! Or maybe Cryphonectria parasitica. Those are my guesses ^_^

But I can ID a few nematophagus fungi from their hyphal traps. And Phymatotrichopsis omnivorum has those characteristic cruiciform branches that pop out pretty well...

And... um.... thats about all I can think of off the top of my head without getting into spore morphological nonsense. Much easier to spend a few hours doing an ITS run and handing it off to the sequencing lab :grin:.


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OfflineLeon76
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Re: Can mushrooms be positively identified through their mycelium? [Re: matsc]
    #21775976 - 06/07/15 08:42 PM (8 years, 7 months ago)

Police found a few mycelium jars of mine. No shrooms or anything, just jars and some spore syringes. I am wondering if I deny that they are cubensis will they have them dna sequenced? Would they in turn have to dna sequence every jar they found? How cost effective is this going to be for them? My plan is to deny, for the present, that they are indeed cubensis. It seems most everyone around here, including the police and attorneys, dont know shit about this. By the questions the detective was asking me I could tell he was pretty clueless about this stuff. He actually asked me if I would be willing to teach him more about the process and he would help me out in return. Naturally I told him top go f himself but since I have come to realize the police are ignorant I am wondering if I should tell them they are legal edible mushrooms.

P.S. They found eight jars


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“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe


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Invisible36fuckin5
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
    #21776028 - 06/07/15 08:53 PM (8 years, 7 months ago)

Don't tell them anything at all. Talk to your lawyer and only your lawyer. They'll probably send at least a sample off.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineLeon76
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Registered: 05/18/13
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Re: Can mushrooms be positively identified through their mycelium? [Re: 36fuckin5]
    #21776090 - 06/07/15 09:06 PM (8 years, 7 months ago)

Yes, I assume they will send a sample off. My question is can it be identified using a reasonable cost procedure. I cant see them spending thousands of dollars to dna sequence some mushroom jars. I have been charged with manufacturing but even the lead detective told me he knew i wasnt selling anything as they didnt see ANY traffic coming and going and I have never sold a single thing. I am strictly a consumer. Apparently they staked out my house for 6 weeks or so thinking I was growing massive amounts of pot. A neighbor saw all my Reaper and Bhutlah peppers in the window that I had started indoors for the summer planting season and called the police and told them I was growing smoke. They had a warrant for marijuana and didnt find anything worth charging me but while destroying my house found the shroom products and charged me. My lawyer is sure that I can get this down to a simple possession charge, if not dismissed entirely, as they didnt find much at all. So I am wondering if it is worth their time and cost for this all to come down to a simple possession charge.


--------------------
“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe


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Offlinematsc
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Re: Can mushrooms be positively identified through their mycelium? [Re: Leon76]
    #21776120 - 06/07/15 09:14 PM (8 years, 7 months ago)

I once had the cops sicced on my apartment because of my chili plants. Someone saw my grow light through the window and called it in. Very annoying.


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OfflineLeon76
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Re: Can mushrooms be positively identified through their mycelium? [Re: matsc]
    #21776383 - 06/07/15 10:16 PM (8 years, 7 months ago)

Thats exactly what happened to me. I would take them out in the morning when the sun was up but here in the Midwest we have late frosts so I would carry them in at night, put them on a dresser directly in front of a window facing the street, and light them up with a couple cfl's until the morning and then do it all over again. Apparently thats suspicious behavior and enough to get a search warrant. I have about 40 pepper plants so I guess they thought i was big time. Man, that pig was so mad when he realized I didnt have a grow operation in my house. If I wasnt so pissed and utterly shocked I would have laughed my ass off. Fucking PIGS!!!!!!!!!!!!!!!!!


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“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe


Edited by Leon76 (06/07/15 10:19 PM)


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OfflineJenn
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Re: Can mushrooms be positively identified through their mycelium? *DELETED* [Re: Leon76]
    #21779232 - 06/08/15 03:11 PM (8 years, 7 months ago)

Post deleted by Jenn

Reason for deletion: I don't like it anymore.



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Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.


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OfflineJenn
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Re: Can mushrooms be positively identified through their mycelium? [Re: Jenn]
    #21783717 - 06/09/15 03:19 PM (8 years, 7 months ago)



--------------------
Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.


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OfflineJenn
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Re: Can mushrooms be positively identified through their mycelium? [Re: Jenn]
    #21798303 - 06/12/15 04:20 PM (8 years, 7 months ago)

Update?


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Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.


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Invisiblenooneman
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Re: Can mushrooms be positively identified through their mycelium? [Re: Jenn]
    #21798398 - 06/12/15 04:34 PM (8 years, 7 months ago)

Preview of coming attractions: OP will be charged with manufacture, distribution and possession of a controlled substance. They won't have it identified by a mycologist. Instead, they'll send it over to a chem lab, and it'll test positive for psilocybin.


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Invisiblemaddchef
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Re: Can mushrooms be positively identified through their mycelium? [Re: nooneman]
    #21798540 - 06/12/15 05:00 PM (8 years, 7 months ago)

Waait. So they staked out your house for 6 weeks and saw no evidence of drug activities but somehow got a warrant to search your house? Seriously wtf.


Something is missing from this story


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Edited by maddchef (06/12/15 05:00 PM)


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OfflineJenn
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Re: Can mushrooms be positively identified through their mycelium? [Re: maddchef]
    #21802074 - 06/13/15 11:02 AM (8 years, 7 months ago)

Well, hopefully Op comes back and hooks us up with the dets...  I love shit like this!


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Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.


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OfflineLeon76
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Re: Can mushrooms be positively identified through their mycelium? [Re: Jenn]
    #22124891 - 08/21/15 10:43 PM (8 years, 5 months ago)

Well, heres an update for those that asked. My 14 year old son, along with his mother, my ex-girlfriend, turned me in. My son broke into my locked grow room one night, took pictures, gave them to his mother and his mother called the police. They apparently did a trash search and found some discarded leaves from the pot and that was enough for the warrant. I have been charged with manufacturing a controlled substance but am getting accepted into drug court so no jail time. But, they COULD NOT test the jars of mycelium or the liquid cultures I had. I was originally charged with manufacturing marijuana as well as manufacturing mushrooms but as the lab was not able to test the jars, and they tried, the manufacturing of mushrooms charge was dismissed leaving only the marijuana charge. My own fucking son. I am still in shock about that. But again, they could not test the mycelium or the LQ's so they kicked that charge. Thought that would be pretty interesting for some of you. Not sure why they couldn't, just know the D.A. said they were unable. So I have to be a good boy for the next 12 to 18 months and not dabble in anything, which really sucks ass, but it is much better than the penitentiary.


--------------------
“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe


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