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Invisibleoontribe
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Registered: 01/14/15
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how long sterile water can stay sterile? making a syringe
    #21762666 - 06/04/15 06:31 PM (8 years, 7 months ago)

so i have decided to follow this tek:
http://www.shroomery.org/forums/showflat.php/Number/9526135

but i have made a mistake...i didnt cover the jar!
and i took the jar from the pc while it was still hot and put it in the SAB because i wanted to use the pc again.
then i covered the jar holes with foil cleaned with alcohol.
i cant make a spore syringe yet because the water was so hot and i am waiting for it to cool down.

is it gonna be still sterile after an hour or something?


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Invisiblemaddchef
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Re: how long sterile water can stay sterile? making a syringe [Re: oontribe]
    #21762796 - 06/04/15 07:00 PM (8 years, 7 months ago)

If it was sterile to begin with then it will stay sterile.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisibleoontribe
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Re: how long sterile water can stay sterile? making a syringe [Re: maddchef]
    #21762829 - 06/04/15 07:09 PM (8 years, 7 months ago)

i sterilized the water inside a jar in a pc!


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Invisiblemaddchef
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Re: how long sterile water can stay sterile? making a syringe [Re: oontribe]
    #21762852 - 06/04/15 07:14 PM (8 years, 7 months ago)

Then it will stay sterile unless you fucked it up somehow


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisibleoontribe
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Registered: 01/14/15
Posts: 3,570
Re: how long sterile water can stay sterile? making a syringe [Re: maddchef]
    #21762861 - 06/04/15 07:16 PM (8 years, 7 months ago)

well i didnt cover the jar lid...which have inoculation holes and i removed it from the pc to sab...can this brief exposition to open air between the PC and the SAB cause contamination?


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: how long sterile water can stay sterile? making a syringe [Re: oontribe]
    #21763006 - 06/04/15 07:50 PM (8 years, 7 months ago)

As long as you have a filter it doesn't matter.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisibleoontribe
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Registered: 01/14/15
Posts: 3,570
Re: how long sterile water can stay sterile? making a syringe [Re: maddchef]
    #21763058 - 06/04/15 08:04 PM (8 years, 7 months ago)

I dont...


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: how long sterile water can stay sterile? making a syringe [Re: oontribe]
    #21763107 - 06/04/15 08:15 PM (8 years, 7 months ago)

Micropore tape? Anything? Its going to suck in air as it cools. Spores live in air. I wouldn't trust it without some kind of filter.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisibleoontribe
Male User Gallery
Registered: 01/14/15
Posts: 3,570
Re: how long sterile water can stay sterile? making a syringe [Re: maddchef]
    #21763125 - 06/04/15 08:20 PM (8 years, 7 months ago)

No filter at all...lol I think i am gonna toss it then.
thx man.


Edited by oontribe (06/04/15 08:29 PM)


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