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OfflineMidwestShroomin
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Can I inoculate in an open room? Any teks?
    #21761261 - 06/04/15 11:28 AM (8 years, 7 months ago)

I live in a loft with no rooms besides the bathroom and a closet (not an option for inoculation due to the girlfriends clothes all over the place).

Basically I have a studio.

I read RR say how bathrooms are the 2nd worse place to do innoculations.

My choices are a small bathroom, or the 'living room'

Which one?

Also, please don't mention a SAB, I'd prefer not to get one due to not having the tools to get it nor the funds


--------------------
"A good traveller has no fixed plans and is not intent on arriving."

-Lao Tzu


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InvisibleMyconin
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Re: Can I inoculate in an open room? Any teks? [Re: MidwestShroomin]
    #21761267 - 06/04/15 11:31 AM (8 years, 7 months ago)

http://www.shroomery.org/11402/Fast-easy-fool-proof-inoculation

But you really should try to make a SAB, It's super cheap, like 10-15$ or less really, and it'll help your game by leaps and bounds.


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Invisiblemushmagic
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761281 - 06/04/15 11:36 AM (8 years, 7 months ago)

:whathesaid:

Tubs are super cheap and you can just melt holes in it if need be. You may not want to right now but it would be worth it in the long run if you plan on moving to anything else.


--------------------


Trade list in journal (partially under construction; more to be added)


Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.


Edited by mushmagic (06/04/15 11:41 AM)


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Offlineqwertyiop25
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Re: Can I inoculate in an open room? Any teks? [Re: MidwestShroomin]
    #21761285 - 06/04/15 11:38 AM (8 years, 7 months ago)

Before I made an SAB, I did all my inoculations in my bathtub after giving it a once over with disinfectant. It's not ideal, but it can work. If you're just using a syringe you should be alright but if you're doing G2G you'll really want an SAB. You can make a simple still air box by just cutting two arm holes in whatever size plastic storage tub you feel like would work best for you. I've even used a tub left-over from a monotub and just taped over the other holes.


--------------------
I'd rather live on the side of a mountain than wander through canyons of concrete and steel.
I'd rather laugh in the rain and sunshine and lay down at sundown in some starry field.


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Invisiblemush madness
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761290 - 06/04/15 11:38 AM (8 years, 7 months ago)

Just put a self-healing injection port  and a synthetic filter in your lid and you will be fine, remember to flame the needle and do not wipe with alcohol after


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Onlinerxb
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Re: Can I inoculate in an open room? Any teks? [Re: mush madness]
    #21761365 - 06/04/15 11:57 AM (8 years, 7 months ago)

:whathesaid:

i dont think injecting through the sponge is a great idea unless the sponge has been soaking in alcohol forever.

but either way its not needed.

use the SHIP.. flame the needle, go in hot... when you pull back out put a dab of high temp silicone on the SHIP where you injected. and you are good :smile:


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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OfflineMatt87
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
    #21761370 - 06/04/15 11:59 AM (8 years, 7 months ago)

I do everything in open air! Be cautious and be brave!!


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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InvisibleMad Season
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761393 - 06/04/15 12:06 PM (8 years, 7 months ago)

And I do everything in a still air box! Why would you risk it? It's so easy to do sab work lol


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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AMU Q&A
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OfflinePussyFart
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761412 - 06/04/15 12:12 PM (8 years, 7 months ago)

Quote:

Matt87 said:
I do everything in open air! Be cautious and be brave!!



How can u be cautious in open air?

Using a SAB is the definition of being cautious.....


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OfflineMatt87
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761422 - 06/04/15 12:15 PM (8 years, 7 months ago)

Just trying to reassure Midwest shrooming it will be OK. There is a great old post on here about mass production and how 95% of the members here are just being overly anal about this shit. SAB isn't a necessity but if you start out with one and have success you probably just use it forever. I started without and see no need to make one now.


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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InvisibleMad Season
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761429 - 06/04/15 12:17 PM (8 years, 7 months ago)

That's because you haven't expanded beyond injecting jars. I started in open air and worked my way to using a sab due to failures.

Kind of done with failures. What's wrong with being anal?


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/04/15 12:18 PM)


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OfflineMatt87
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761438 - 06/04/15 12:20 PM (8 years, 7 months ago)

Quote:

Mad Season said:
That's because you haven't expanded beyond injecting jars. I started in open air and worked my way to using a sab due to failures.

Kind of done with failures. What's wrong with being anal?



Says who? I've only been in this for less than a year, but I've done a couple hundred g2gs. Only had to throw out three jars of wbs thus far.


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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OfflineMatt87
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761454 - 06/04/15 12:24 PM (8 years, 7 months ago)

AND the guy said he didn't have money for a SAB. Fifteen dollars though it may be. I was just answering his question to the best of my abilities. Not saying open air transfers are equally safe by any means, but OP shouldn't let not having a SAB keep him from enjoying this hobby!


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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InvisibleMad Season
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761466 - 06/04/15 12:27 PM (8 years, 7 months ago)

:thumbup: I stopped doing g2gs long ago. I did a side by side with a2g and a2g kicked its ass hard. If you want to step up your game do some agar, and do it in open air. I dare you. I'm tired of hidden contams, and your luck will eventually run out, like everyone else's. Including myself. Sure I had some success in open air. Looking back though I wouldn't call it success.

Would you rather be the guy who turned failures to success by doing it right? Or the guy who went from success to failures doing it wrong? Sorry but it's just the truth. I'm a cheap mofo but I'd never cheap out on an sab. Do it right, all will be well. Then you'd never have failures to fix in the first place.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/04/15 12:28 PM)


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InvisibleMyconin
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761502 - 06/04/15 12:37 PM (8 years, 7 months ago)



--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Onlinerxb
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761507 - 06/04/15 12:38 PM (8 years, 7 months ago)

you can use a clear plastic garbage bag.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Invisiblemush madness
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761523 - 06/04/15 12:43 PM (8 years, 7 months ago)

Sab is definitely the way to go, i do all my work in one just because I cant afford a hood right now

But he ask if it could be done, and the answer is yes.

It may not be ideal but if you are shooting a dirty ass MS syringe into grain what is a SAB going to help


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OfflinePussyFart
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761533 - 06/04/15 12:45 PM (8 years, 7 months ago)

Quote:

Matt87 said:
Just trying to reassure Midwest shrooming it will be OK. There is a great old post on here about mass production and how 95% of the members here are just being overly anal about this shit. SAB isn't a necessity but if you start out with one and have success you probably just use it forever. I started without and see no need to make one now.



If you had an extremely bright flashlight you might rethink that......there is so much shit just floating around in the air......shit we can see with a simple light....not to mention the shit we can't see.....

I have had entire G2G transfers go bad because of shitty technique in my SAB, so why would I even want to try open air?

I choose extra, simple steps as precautionary measures.....it's just like carrying a gun, or wearing a seatbelt, or getting health insurance.....it's just making sure ur ass is covered.


Edited by PussyFart (06/04/15 12:46 PM)


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OfflineFreeWorldOrder
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Re: Can I inoculate in an open room? Any teks? [Re: PussyFart]
    #21761550 - 06/04/15 12:50 PM (8 years, 7 months ago)

Maybe for the basic PF-Tek .... but when advancing to g2g, agar work (cloning, isolating, etc) Give yourself & the hobby the respect it deserves and make a $10 SAB for crying out loud!.... if someone can afford to get into the hobby (inexpensive) then they can surely break down and make a SAB.... GEEEEZ!


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK


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Onlinerxb
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Re: Can I inoculate in an open room? Any teks? [Re: FreeWorldOrder]
    #21761559 - 06/04/15 12:52 PM (8 years, 7 months ago)

you can do almost everything you can do in a SAB in a clear trashbag, for $1

SAB is nice tho.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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OfflineFreeWorldOrder
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Re: Can I inoculate in an open room? Any teks? [Re: FreeWorldOrder]
    #21761560 - 06/04/15 12:52 PM (8 years, 7 months ago)

.... that too!....lol.


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK


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InvisibleMad Season
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
    #21761565 - 06/04/15 12:53 PM (8 years, 7 months ago)

Hell people just use a cardboard box with 1 side wide open to block the area from 5 other sides. It's still better than open air lol


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblemush madness
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
    #21761572 - 06/04/15 12:55 PM (8 years, 7 months ago)

Quote:

rxb said:
you can do almost everything you can do in a SAB in a clear trashbag, for $1

SAB is nice tho.




I saw a site selling them for 30 bucks once, but they had cute little gloves built in :goodluckwiththat2:


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Onlinerxb
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761590 - 06/04/15 12:58 PM (8 years, 7 months ago)

Quote:

Mad Season said:
Hell people just use a cardboard box with 1 side wide open to block the area from 5 other sides. It's still better than open air lol




:thatsayes:


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Invisibleslapphappypill
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Re: Can I inoculate in an open room? Any teks? [Re: mush madness]
    #21761598 - 06/04/15 12:59 PM (8 years, 7 months ago)

Check out my tech in my signature. I built a pvc pipe room that hung from my ceiling. I lysol bombed it and was able to do open air agar work to bulk spawns to mono tubs :-)


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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InvisibleMyconin
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
    #21761608 - 06/04/15 01:00 PM (8 years, 7 months ago)

Quote:

rxb said:
you can do almost everything you can do in a SAB in a clear trashbag, for $1

SAB is nice tho.




The problem with a bag is that is constantly moves as you move around, negating the "still air" one is trying to achieve. You'd still be mixing all the contams in the air into the "clean" environment. Counter-productive if you ask me.


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Invisibleslapphappypill
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761624 - 06/04/15 01:03 PM (8 years, 7 months ago)



--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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InvisibleMyconin
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Re: Can I inoculate in an open room? Any teks? [Re: slapphappypill]
    #21761643 - 06/04/15 01:07 PM (8 years, 7 months ago)

Quote:

slapphappypill said:
http://www.shroomery.org/forums/showflat.php/Number/18019536




I had this image in my head of a guy laying a garbage bag on it's side, placing all materials needed inside, and then putting their arms up into the bag to work. Even with gloves and all the other precautions, if you're doing that you may as well do open air imo.

That link though, now that's something to consider :strokebeard:


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Onlinerxb
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761662 - 06/04/15 01:13 PM (8 years, 7 months ago)

lay bag on its side.

spray everything down with alcohol. and put in bag.

spray bag down with alcohol.

roll up/ tape up end of bag....

do work through the bag... becareful not to tear the plastic opening jars you can pre loosen the lid, just dont loosen it all the way.

its pretty much the same thing as a SAB.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisibleMad Season
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
    #21761667 - 06/04/15 01:14 PM (8 years, 7 months ago)

Shmuvbox? :P that's a bag utilization too. I'm just going to stick with my still air box haha.

I use folgers coffee cans to heat my arm holes that way I can have folger holes :wink:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/04/15 01:17 PM)


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Onlinerxb
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761673 - 06/04/15 01:16 PM (8 years, 7 months ago)

yeah shmuvebox = bag taped to a hepafilter/ioniser to make a small posative pressure room... he tears holes in the bag.

the tek i mentioned, the bag is closed.... sort of sealed.

you dont need air in the bag you can just work...

its the same level of safty as a SAB with no bulk. (because the air is not sterile its just still)


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisibleMad Season
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
    #21761683 - 06/04/15 01:18 PM (8 years, 7 months ago)

Haha I know what you meant. I was just saying another way of using bags. Bags are cool. You're right it's called a still air box. Not a sterile box :P so it's the same thing.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineShroominMe
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761701 - 06/04/15 01:24 PM (8 years, 7 months ago)

I use my bathroom because I foster animals and the rest of my place is not exactly sterile.  The bathroom is good because it does not have carpet, is small and can be easily sterilized.

I first remove all the towels and anything loose sitting on my sink.  Next I start my HEPA air filter on max velocity, close the door and wait for 1/2 hr - 1 hr.

Then I spray everything - ceiling, walls, shower curtain, liner, toilet, sink, floor - with a bleach solution 2T bleach to 32 ozs water using a spray bottle.  I used to use bleach in a bucket but the spray bottle is so much easier.  I don't rush this part.  The bleach gets to me after a while so I take 3 or 4 passes to get it done.

After that I put a quarter cup of bleach in the toilet bowl and another quarter cup in the water reservoir.  By that time things are pretty sterile so I bring in my SAB which is just a cardboard box with 6" circles cut out 3/4 on the side for my arms.  The fact that I haven't completely cut out the circles leaves flaps to loosely close the SAB.  The top is just plastic wrap.  Total cost basically $0.

I spray all the equipment I am going to use in my SAB with Lysol and place inside the SAB.  Then I spray all sides of the SAB with Lysol and then spray the inside pretty well.  I make sure to get the top inside of the plastic wrap as well as the sides and bottom.  The SAB sits on my sink.

Then, outside of my bathroom, I wash myself with disinfectant soap, put on clean cotton t-shirt and shorts, and spray myself with Lysol.  Once I am back in the bathroom I spray the bottoms of my feet and then quickly spray the SAB and surrounding area one more time for good luck.  Wash my hands and arms with rubbing alcohol, let dry, put on gloves, wash gloves with rubbing alcohol, and begin.

Sounds like a lot of work, I guess, but since I've been doing this I've had 0 contams.  Before I had more contams than success.

Maybe it will help to consider the disinfection process like a sacred ceremony necessary to produce the sacred fruits :smile:


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Re: Can I inoculate in an open room? Any teks? [Re: ShroominMe]
    #21761718 - 06/04/15 01:29 PM (8 years, 7 months ago)

Your predicament is the same as mine. Old farm house with a bunch of animals. I do my work in the living room watching TV or in the kitchen. Just be really still and never let your hand or arm go over any open media. Dont jostle around because particles take 20 minutes to hit the ground. Techniques will vastly outweigh cleanliness. Especially in an unclean box to begin with.


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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761750 - 06/04/15 01:39 PM (8 years, 7 months ago)

Quote:

Myconin said:
Quote:

slapphappypill said:
http://www.shroomery.org/forums/showflat.php/Number/18019536




I had this image in my head of a guy laying a garbage bag on it's side, placing all materials needed inside, and then putting their arms up into the bag to work. Even with gloves and all the other precautions, if you're doing that you may as well do open air imo.

That link though, now that's something to consider :strokebeard:




It served me very well until I got a 4x2 flowhood :datass:


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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21762040 - 06/04/15 03:07 PM (8 years, 7 months ago)

thats why you spray the inside of the bag down with alcohol.

but its really not a big deal.

neither is a SAB but its $1 vs $10

not much contamination in a bag, you spray it all down so theres less.

shrug.

none of it is a flow hood.


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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
    #21762067 - 06/04/15 03:14 PM (8 years, 7 months ago)

dont listen to me im not that experienced but i do open air in my bathroom after disinfecting spraying the whole room flame sterilize and and alcohol no contams yet


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Re: Can I inoculate in an open room? Any teks? [Re: bugman]
    #21762075 - 06/04/15 03:16 PM (8 years, 7 months ago)

Quote:

bugman said:
dont listen to me



K


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Re: Can I inoculate in an open room? Any teks? [Re: PussyFart]
    #21764814 - 06/05/15 07:19 AM (8 years, 7 months ago)

:lmafo:


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