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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 9 hours
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Re: Can I inoculate in an open room? Any teks? [Re: FreeWorldOrder]
#21761560 - 06/04/15 12:52 PM (8 years, 7 months ago) |
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.... that too!....lol.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
#21761565 - 06/04/15 12:53 PM (8 years, 7 months ago) |
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Hell people just use a cardboard box with 1 side wide open to block the area from 5 other sides. It's still better than open air lol
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mush madness
absorbing everything



Registered: 05/22/15
Posts: 252
Loc: Brazil
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
#21761572 - 06/04/15 12:55 PM (8 years, 7 months ago) |
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Quote:
rxb said: you can do almost everything you can do in a SAB in a clear trashbag, for $1
SAB is nice tho.
I saw a site selling them for 30 bucks once, but they had cute little gloves built in
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,526
Loc: FREE PSYCHONAUTICA
Last seen: 11 seconds
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
#21761590 - 06/04/15 12:58 PM (8 years, 7 months ago) |
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Quote:
Mad Season said: Hell people just use a cardboard box with 1 side wide open to block the area from 5 other sides. It's still better than open air lol
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: Can I inoculate in an open room? Any teks? [Re: mush madness]
#21761598 - 06/04/15 12:59 PM (8 years, 7 months ago) |
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Check out my tech in my signature. I built a pvc pipe room that hung from my ceiling. I lysol bombed it and was able to do open air agar work to bulk spawns to mono tubs :-)
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
#21761608 - 06/04/15 01:00 PM (8 years, 7 months ago) |
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Quote:
rxb said: you can do almost everything you can do in a SAB in a clear trashbag, for $1
SAB is nice tho.
The problem with a bag is that is constantly moves as you move around, negating the "still air" one is trying to achieve. You'd still be mixing all the contams in the air into the "clean" environment. Counter-productive if you ask me.
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
#21761624 - 06/04/15 01:03 PM (8 years, 7 months ago) |
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-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Re: Can I inoculate in an open room? Any teks? [Re: slapphappypill]
#21761643 - 06/04/15 01:07 PM (8 years, 7 months ago) |
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Quote:
slapphappypill said: http://www.shroomery.org/forums/showflat.php/Number/18019536
I had this image in my head of a guy laying a garbage bag on it's side, placing all materials needed inside, and then putting their arms up into the bag to work. Even with gloves and all the other precautions, if you're doing that you may as well do open air imo.
That link though, now that's something to consider
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,526
Loc: FREE PSYCHONAUTICA
Last seen: 11 seconds
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
#21761662 - 06/04/15 01:13 PM (8 years, 7 months ago) |
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lay bag on its side.
spray everything down with alcohol. and put in bag.
spray bag down with alcohol.
roll up/ tape up end of bag....
do work through the bag... becareful not to tear the plastic opening jars you can pre loosen the lid, just dont loosen it all the way.
its pretty much the same thing as a SAB.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
#21761667 - 06/04/15 01:14 PM (8 years, 7 months ago) |
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Shmuvbox? :P that's a bag utilization too. I'm just going to stick with my still air box haha.
I use folgers coffee cans to heat my arm holes that way I can have folger holes
Edited by Mad Season (06/04/15 01:17 PM)
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,526
Loc: FREE PSYCHONAUTICA
Last seen: 11 seconds
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
#21761673 - 06/04/15 01:16 PM (8 years, 7 months ago) |
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yeah shmuvebox = bag taped to a hepafilter/ioniser to make a small posative pressure room... he tears holes in the bag.
the tek i mentioned, the bag is closed.... sort of sealed.
you dont need air in the bag you can just work...
its the same level of safty as a SAB with no bulk. (because the air is not sterile its just still)
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
#21761683 - 06/04/15 01:18 PM (8 years, 7 months ago) |
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Haha I know what you meant. I was just saying another way of using bags. Bags are cool. You're right it's called a still air box. Not a sterile box :P so it's the same thing.
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ShroominMe
Stranger
Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
#21761701 - 06/04/15 01:24 PM (8 years, 7 months ago) |
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I use my bathroom because I foster animals and the rest of my place is not exactly sterile. The bathroom is good because it does not have carpet, is small and can be easily sterilized.
I first remove all the towels and anything loose sitting on my sink. Next I start my HEPA air filter on max velocity, close the door and wait for 1/2 hr - 1 hr.
Then I spray everything - ceiling, walls, shower curtain, liner, toilet, sink, floor - with a bleach solution 2T bleach to 32 ozs water using a spray bottle. I used to use bleach in a bucket but the spray bottle is so much easier. I don't rush this part. The bleach gets to me after a while so I take 3 or 4 passes to get it done.
After that I put a quarter cup of bleach in the toilet bowl and another quarter cup in the water reservoir. By that time things are pretty sterile so I bring in my SAB which is just a cardboard box with 6" circles cut out 3/4 on the side for my arms. The fact that I haven't completely cut out the circles leaves flaps to loosely close the SAB. The top is just plastic wrap. Total cost basically $0.
I spray all the equipment I am going to use in my SAB with Lysol and place inside the SAB. Then I spray all sides of the SAB with Lysol and then spray the inside pretty well. I make sure to get the top inside of the plastic wrap as well as the sides and bottom. The SAB sits on my sink.
Then, outside of my bathroom, I wash myself with disinfectant soap, put on clean cotton t-shirt and shorts, and spray myself with Lysol. Once I am back in the bathroom I spray the bottoms of my feet and then quickly spray the SAB and surrounding area one more time for good luck. Wash my hands and arms with rubbing alcohol, let dry, put on gloves, wash gloves with rubbing alcohol, and begin.
Sounds like a lot of work, I guess, but since I've been doing this I've had 0 contams. Before I had more contams than success.
Maybe it will help to consider the disinfection process like a sacred ceremony necessary to produce the sacred fruits
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Can I inoculate in an open room? Any teks? [Re: ShroominMe]
#21761718 - 06/04/15 01:29 PM (8 years, 7 months ago) |
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Your predicament is the same as mine. Old farm house with a bunch of animals. I do my work in the living room watching TV or in the kitchen. Just be really still and never let your hand or arm go over any open media. Dont jostle around because particles take 20 minutes to hit the ground. Techniques will vastly outweigh cleanliness. Especially in an unclean box to begin with.
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
#21761750 - 06/04/15 01:39 PM (8 years, 7 months ago) |
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Quote:
Myconin said:
Quote:
slapphappypill said: http://www.shroomery.org/forums/showflat.php/Number/18019536
I had this image in my head of a guy laying a garbage bag on it's side, placing all materials needed inside, and then putting their arms up into the bag to work. Even with gloves and all the other precautions, if you're doing that you may as well do open air imo.
That link though, now that's something to consider 
It served me very well until I got a 4x2 flowhood
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,526
Loc: FREE PSYCHONAUTICA
Last seen: 11 seconds
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
#21762040 - 06/04/15 03:07 PM (8 years, 7 months ago) |
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thats why you spray the inside of the bag down with alcohol.
but its really not a big deal.
neither is a SAB but its $1 vs $10
not much contamination in a bag, you spray it all down so theres less.
shrug.
none of it is a flow hood.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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bugman
buggy


Registered: 05/10/15
Posts: 245
Loc: united kingdom
Last seen: 4 months, 20 days
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
#21762067 - 06/04/15 03:14 PM (8 years, 7 months ago) |
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dont listen to me im not that experienced but i do open air in my bathroom after disinfecting spraying the whole room flame sterilize and and alcohol no contams yet
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 20 hours
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Re: Can I inoculate in an open room? Any teks? [Re: bugman]
#21762075 - 06/04/15 03:16 PM (8 years, 7 months ago) |
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Quote:
bugman said: dont listen to me
K
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 9 hours
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Re: Can I inoculate in an open room? Any teks? [Re: PussyFart]
#21764814 - 06/05/15 07:19 AM (8 years, 7 months ago) |
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-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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