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OfflineMidwestShroomin
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Registered: 05/21/15
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Can I inoculate in an open room? Any teks?
    #21761261 - 06/04/15 11:28 AM (8 years, 7 months ago)

I live in a loft with no rooms besides the bathroom and a closet (not an option for inoculation due to the girlfriends clothes all over the place).

Basically I have a studio.

I read RR say how bathrooms are the 2nd worse place to do innoculations.

My choices are a small bathroom, or the 'living room'

Which one?

Also, please don't mention a SAB, I'd prefer not to get one due to not having the tools to get it nor the funds


--------------------
"A good traveller has no fixed plans and is not intent on arriving."

-Lao Tzu


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InvisibleMyconin
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Registered: 05/11/15
Posts: 308
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Re: Can I inoculate in an open room? Any teks? [Re: MidwestShroomin]
    #21761267 - 06/04/15 11:31 AM (8 years, 7 months ago)

http://www.shroomery.org/11402/Fast-easy-fool-proof-inoculation

But you really should try to make a SAB, It's super cheap, like 10-15$ or less really, and it'll help your game by leaps and bounds.


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Invisiblemushmagic
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761281 - 06/04/15 11:36 AM (8 years, 7 months ago)

:whathesaid:

Tubs are super cheap and you can just melt holes in it if need be. You may not want to right now but it would be worth it in the long run if you plan on moving to anything else.


--------------------


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Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.


Edited by mushmagic (06/04/15 11:41 AM)


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Offlineqwertyiop25
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Re: Can I inoculate in an open room? Any teks? [Re: MidwestShroomin]
    #21761285 - 06/04/15 11:38 AM (8 years, 7 months ago)

Before I made an SAB, I did all my inoculations in my bathtub after giving it a once over with disinfectant. It's not ideal, but it can work. If you're just using a syringe you should be alright but if you're doing G2G you'll really want an SAB. You can make a simple still air box by just cutting two arm holes in whatever size plastic storage tub you feel like would work best for you. I've even used a tub left-over from a monotub and just taped over the other holes.


--------------------
I'd rather live on the side of a mountain than wander through canyons of concrete and steel.
I'd rather laugh in the rain and sunshine and lay down at sundown in some starry field.


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Invisiblemush madness
absorbing everything
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Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761290 - 06/04/15 11:38 AM (8 years, 7 months ago)

Just put a self-healing injection port  and a synthetic filter in your lid and you will be fine, remember to flame the needle and do not wipe with alcohol after


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Offlinerxb
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Re: Can I inoculate in an open room? Any teks? [Re: mush madness]
    #21761365 - 06/04/15 11:57 AM (8 years, 7 months ago)

:whathesaid:

i dont think injecting through the sponge is a great idea unless the sponge has been soaking in alcohol forever.

but either way its not needed.

use the SHIP.. flame the needle, go in hot... when you pull back out put a dab of high temp silicone on the SHIP where you injected. and you are good :smile:


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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OfflineMatt87
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Re: Can I inoculate in an open room? Any teks? [Re: rxb]
    #21761370 - 06/04/15 11:59 AM (8 years, 7 months ago)

I do everything in open air! Be cautious and be brave!!


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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InvisibleMad Season
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761393 - 06/04/15 12:06 PM (8 years, 7 months ago)

And I do everything in a still air box! Why would you risk it? It's so easy to do sab work lol


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinePussyFart
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761412 - 06/04/15 12:12 PM (8 years, 7 months ago)

Quote:

Matt87 said:
I do everything in open air! Be cautious and be brave!!



How can u be cautious in open air?

Using a SAB is the definition of being cautious.....


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OfflineMatt87
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761422 - 06/04/15 12:15 PM (8 years, 7 months ago)

Just trying to reassure Midwest shrooming it will be OK. There is a great old post on here about mass production and how 95% of the members here are just being overly anal about this shit. SAB isn't a necessity but if you start out with one and have success you probably just use it forever. I started without and see no need to make one now.


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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InvisibleMad Season
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761429 - 06/04/15 12:17 PM (8 years, 7 months ago)

That's because you haven't expanded beyond injecting jars. I started in open air and worked my way to using a sab due to failures.

Kind of done with failures. What's wrong with being anal?


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/04/15 12:18 PM)


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OfflineMatt87
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761438 - 06/04/15 12:20 PM (8 years, 7 months ago)

Quote:

Mad Season said:
That's because you haven't expanded beyond injecting jars. I started in open air and worked my way to using a sab due to failures.

Kind of done with failures. What's wrong with being anal?



Says who? I've only been in this for less than a year, but I've done a couple hundred g2gs. Only had to throw out three jars of wbs thus far.


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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OfflineMatt87
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761454 - 06/04/15 12:24 PM (8 years, 7 months ago)

AND the guy said he didn't have money for a SAB. Fifteen dollars though it may be. I was just answering his question to the best of my abilities. Not saying open air transfers are equally safe by any means, but OP shouldn't let not having a SAB keep him from enjoying this hobby!


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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InvisibleMad Season
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761466 - 06/04/15 12:27 PM (8 years, 7 months ago)

:thumbup: I stopped doing g2gs long ago. I did a side by side with a2g and a2g kicked its ass hard. If you want to step up your game do some agar, and do it in open air. I dare you. I'm tired of hidden contams, and your luck will eventually run out, like everyone else's. Including myself. Sure I had some success in open air. Looking back though I wouldn't call it success.

Would you rather be the guy who turned failures to success by doing it right? Or the guy who went from success to failures doing it wrong? Sorry but it's just the truth. I'm a cheap mofo but I'd never cheap out on an sab. Do it right, all will be well. Then you'd never have failures to fix in the first place.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/04/15 12:28 PM)


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InvisibleMyconin
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Registered: 05/11/15
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Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761502 - 06/04/15 12:37 PM (8 years, 7 months ago)



--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Offlinerxb
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Registered: 08/24/13
Posts: 9,521
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Re: Can I inoculate in an open room? Any teks? [Re: Myconin]
    #21761507 - 06/04/15 12:38 PM (8 years, 7 months ago)

you can use a clear plastic garbage bag.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Invisiblemush madness
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Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: Can I inoculate in an open room? Any teks? [Re: Mad Season]
    #21761523 - 06/04/15 12:43 PM (8 years, 7 months ago)

Sab is definitely the way to go, i do all my work in one just because I cant afford a hood right now

But he ask if it could be done, and the answer is yes.

It may not be ideal but if you are shooting a dirty ass MS syringe into grain what is a SAB going to help


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OfflinePussyFart
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Re: Can I inoculate in an open room? Any teks? [Re: Matt87]
    #21761533 - 06/04/15 12:45 PM (8 years, 7 months ago)

Quote:

Matt87 said:
Just trying to reassure Midwest shrooming it will be OK. There is a great old post on here about mass production and how 95% of the members here are just being overly anal about this shit. SAB isn't a necessity but if you start out with one and have success you probably just use it forever. I started without and see no need to make one now.



If you had an extremely bright flashlight you might rethink that......there is so much shit just floating around in the air......shit we can see with a simple light....not to mention the shit we can't see.....

I have had entire G2G transfers go bad because of shitty technique in my SAB, so why would I even want to try open air?

I choose extra, simple steps as precautionary measures.....it's just like carrying a gun, or wearing a seatbelt, or getting health insurance.....it's just making sure ur ass is covered.


Edited by PussyFart (06/04/15 12:46 PM)


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OfflineFreeWorldOrder
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Re: Can I inoculate in an open room? Any teks? [Re: PussyFart]
    #21761550 - 06/04/15 12:50 PM (8 years, 7 months ago)

Maybe for the basic PF-Tek .... but when advancing to g2g, agar work (cloning, isolating, etc) Give yourself & the hobby the respect it deserves and make a $10 SAB for crying out loud!.... if someone can afford to get into the hobby (inexpensive) then they can surely break down and make a SAB.... GEEEEZ!


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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Offlinerxb
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Re: Can I inoculate in an open room? Any teks? [Re: FreeWorldOrder]
    #21761559 - 06/04/15 12:52 PM (8 years, 7 months ago)

you can do almost everything you can do in a SAB in a clear trashbag, for $1

SAB is nice tho.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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