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Invisiblemaddchef
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Straw steam sterilization
    #21759909 - 06/04/15 12:19 AM (8 years, 7 months ago)

For an experiment I will be running in the next few days is anyone able to give me a semi accurate/starting point timetable of straw sterilization?

Say if one were to loosely fill a qt jar and PC it?

Only I am looking for a guestimate of how long you would steam sterilize said jar for? X minutes @ 212f

Hopefully I'm not looking at 8 hrs like grain. I wouldn't think so especially because I said LOOSE.

Thanks
:havesomescience:


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

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InvisibleImperfect Iam
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Re: Straw steam sterilization [Re: maddchef]
    #21759919 - 06/04/15 12:25 AM (8 years, 7 months ago)

You don't want to sterilize straw, you want to pasteurize it in 140-160 degree water for 1 hour, to an hour and a half.

It is also said it helps straw to retain water by soaking it in an anti bacterial soap before pasteurization.


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OfflineCharleskun
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Re: Straw steam sterilization [Re: Imperfect Iam]
    #21759959 - 06/04/15 12:44 AM (8 years, 7 months ago)

Quote:

Imperfect Iam said:
You don't want to sterilize straw, you want to pasteurize it in 140-160 degree water for 1 hour, to an hour and a half.

It is also said it helps straw to retain water by soaking it in an anti bacterial soap before pasteurization.




:whathesaid:

Just get like a 5 gallon bucket with a lid and put your straw + boiling water in and close the lid, wait about 2 hours and you should be good.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Charleskun]
    #21760566 - 06/04/15 07:31 AM (8 years, 7 months ago)

I understand well proper straw prep and that's not the info I'm after.

I'm asking for my own reasons if you were going to steam sterilize a quart of loose straw how long you would run it for?

I own several PCs, modified trashcan for pasteurization, hood, among other things so I'm not trying to take noob shortcuts.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMyconin
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Re: Straw steam sterilization [Re: maddchef]
    #21760573 - 06/04/15 07:35 AM (8 years, 7 months ago)

If you really want to sterilize it for curiosity's sake, I say err on the side of caution. Go for a 2hr or more run in the PC, although I would guess that the usual 90min run should be ok.

If this is all just an experiment, why not set up a couple different runs for different time periods and see which ones perform optimally?


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Myconin]
    #21760735 - 06/04/15 08:47 AM (8 years, 7 months ago)

It won't be pc'd it will be steam sterilized.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMad Season
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Re: Straw steam sterilization [Re: maddchef]
    #21760741 - 06/04/15 08:49 AM (8 years, 7 months ago)

Pc is steam sterilization. You can't go past 100° with water. It's pressurized steam. You need to specify and say steam sterilization at 1 psi.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Mad Season]
    #21761094 - 06/04/15 10:35 AM (8 years, 7 months ago)

How hot exactly is steam not under pressure? When we tell ppl how to do the pf tek we say steam sterilize when talking about a pot. We say PC when we mean under pressure


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMad Season
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Re: Straw steam sterilization [Re: maddchef]
    #21761118 - 06/04/15 10:47 AM (8 years, 7 months ago)

I've never taken the time to measure the temperature of steam. I don't know what more to tell you, except that most of us who are up to date refer to steam sterilization as pcing. I call pf tek just boiling jars. It's not really even sterilization, to boil and sterilize you have to do it for 8 hours.

To be specific, if it's not under pressure, it's just steam with no pressure or steam at 1 psi.


--------------------
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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Mad Season]
    #21761125 - 06/04/15 10:49 AM (8 years, 7 months ago)

Steam can only get to 212f I believe under no pressure.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
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Re: Straw steam sterilization [Re: maddchef]
    #21761153 - 06/04/15 10:57 AM (8 years, 7 months ago)

Many people have tried sterilized straw, all have failed.


--------------------
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These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: 36fuckin5]
    #21761392 - 06/04/15 12:06 PM (8 years, 7 months ago)

Understood and agreed. I don't plan on sterilizing it and spawning to it like a tub or stuffing a log.

Sterilized and left in its vessel is: jar, bag, tube, etc. It should behave like any other sterile media and be decently resistant once colonized and "birthed" no?

What I have in mind I plan on colonizing it in the container used to sterilize it. Bag for instance, sterilize a straw bag, open in front of a hood and pour in spawn, reseal.

Would this not work in theory?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
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Re: Straw steam sterilization [Re: maddchef]
    #21761405 - 06/04/15 12:09 PM (8 years, 7 months ago)

I suppose that'd work, but it'd be easier to just dump some hot water over it and throw in some lime. Plus you could do big-ass logs or a basket that way.

You may have problems once you introduce FAE though. It'd definitely be a 1-and-done thing.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineGreenRabbit
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Re: Straw steam sterilization [Re: Mad Season]
    #21761493 - 06/04/15 12:33 PM (8 years, 7 months ago)

Quote:

Mad Season said:
Pc is steam sterilization. You can't go past 100° with water. It's pressurized steam. You need to specify and say steam sterilization at 1 psi.




Increasing pressure raises the BP of water, so you can go past 100C. But beyond that, yes, the steam is pressurized as well.

Steam sterilization is not 1 psi. It is 0 psi gage pressure or 1 atm absolute pressure (101 kPa or 14.7 psi).


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InvisibleMad Season
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Re: Straw steam sterilization [Re: GreenRabbit]
    #21761518 - 06/04/15 12:42 PM (8 years, 7 months ago)

Truth. It's also why you can cool an engine with no boiling beyond 100°. However I believe it's like 1 psi ups it by 2 or 3°, and we're relying on the high heat from the steam beyond the 130-140° water to sterilize, thus it's technically steam sterilization. It confused us because that's what we call pcing. It's a lot easier to say steam sterilization at atmospheric pressure. It wouldn't confuse everyone.


--------------------
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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Mad Season]
    #21761539 - 06/04/15 12:47 PM (8 years, 7 months ago)

OK so now that all that has been cleared up. If you were to STEAM not PC a quart or bag of loose straw how long would it take to achieve sterilization? 60,90,120, etc.

Best guesses anyone?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMad Season
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Re: Straw steam sterilization [Re: maddchef]
    #21761547 - 06/04/15 12:49 PM (8 years, 7 months ago)

120 or more.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Mad Season]
    #21761724 - 06/04/15 01:30 PM (8 years, 7 months ago)

Really? I was thinking shorter. We PC solid grains for 90 @ 250ish so I figured with straw being hollow and loose that 212f @ 90mins might cut it.

I dunno what kind of bacteria straw harbors though, I only ever see it succumb to mold.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
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Re: Straw steam sterilization [Re: maddchef]
    #21761740 - 06/04/15 01:35 PM (8 years, 7 months ago)

I'd do it for at least 2 hours. Straw is dirty stuff.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: 36fuckin5]
    #21761743 - 06/04/15 01:37 PM (8 years, 7 months ago)

Would a soak in bleach or lime adjusted water help to kill anything off before sterilization or does that only serve to make it inhabitable for spore germination?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
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Re: Straw steam sterilization [Re: maddchef]
    #21761748 - 06/04/15 01:39 PM (8 years, 7 months ago)

Couldn't hurt.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineGreenRabbit
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Re: Straw steam sterilization [Re: 36fuckin5]
    #21762220 - 06/04/15 04:00 PM (8 years, 7 months ago)

Quote:

36fuckin5 said:
I'd do it for at least 2 hours. Straw is dirty stuff.




I noticed a increase in success rates for steam sterilizing BRF jars when going from 90 minutes to 120. You can steam sterilize for as long as you want, I remember RR saying something like 2 hours at 15psi is equivalent to 8 hours steam sterilization. Don't quote me on that though...

I still don't have 100% success at 2 hours with BRF jars so I'd recommend at least 2.5 to 3 hours.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: GreenRabbit]
    #21762230 - 06/04/15 04:05 PM (8 years, 7 months ago)

True about the 8 hours but that is for grain.

Straw should be a bit easier since it is pretty hollow and won't be dense in my use case.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
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Re: Straw steam sterilization [Re: maddchef]
    #21762271 - 06/04/15 04:25 PM (8 years, 7 months ago)

Try a few of each. :shrug:


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Re: Straw steam sterilization [Re: 36fuckin5]
    #21762496 - 06/04/15 05:34 PM (8 years, 7 months ago)

I get what youre trying to do and its an interesting Idea, like transfering your grains into a bag of sterile straw and letting it colonize invitro


For pasturizing straw Ive found the buckets (like you would use to prep coir) work perfect I just soak my straw in some soapy water overnight and then strain, heat my water to around 180 and pour it in and then cap. After an hour the end temp is usually close to 150 so it works out pretty well for doing smaller batches


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Re: Straw steam sterilization [Re: mushpunx]
    #21762674 - 06/04/15 06:34 PM (8 years, 7 months ago)

Quote:

maddchef said:
Would a soak in bleach or lime adjusted water help to kill anything off before sterilization or does that only serve to make it inhabitable for spore germination?




Don't use bleach. Bleach only kills 99.9%.  A single sneeze droplet contains over.... well... I've got a comic for you.



I don't care what anyone else here says. Endospores are still endospores. If your goal is sterilization then you NEED to sterilize it.  This isn't something that changes just because you're using a less dense medium.  If you want 100% success you should do the 8 hour steaming.  If you only partially sterilize your straw you're setting yourself up for contamination.

And yes residual bleach will affect mycelium unless you can figure out a way to wash it out of the straw later.
Even better though, do some jars at 2 hours, some at 4 hours and some at 8 hours and see which one gets better results.

:aweohyou:


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: elasticaltiger]
    #21762774 - 06/04/15 06:56 PM (8 years, 7 months ago)

The bleach isn't used was a sterilizer in this instance, its used for a major pH swing. Endospores are endospores indeed but the density of your chosen substrate will determine how quickly center mass reaches temp.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblebodhisattaMDiscordReddit
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Re: Straw steam sterilization [Re: 36fuckin5]
    #21762887 - 06/04/15 07:21 PM (8 years, 7 months ago)

Quote:

36fuckin5 said:
Many people have tried sterilized straw, all have failed.



I've done it a few times :shrug:

if it was just straw though I would say at least 60m. depends on how sterilized you want it. what the original contaminant load was too.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: bodhisatta]
    #21762987 - 06/04/15 07:46 PM (8 years, 7 months ago)

Might as well divulge why I'm so curious.

I'm wanting to build something I can sterilize straw logs in. Likely a large diameter pipe.

I would use poly tubing longer than what the original log would be and stuff very loosely. Have one end sealed per usual and possibly have the open end treated like a spawn bag folded up with a sleeve in it.

After sterilization open the open end in front of my hood and dump in spawn, then massage until I get grain to the other end. This should be pretty easily done since the straw will be so loose.

Once spawn is distributed then simply seal and squeeze the log like a backwards tube of toothpaste to compact it to normal density and size, then wrap with a zip tie or bailing wire to hold shape.

I am afraid to make a pressure vessel so I was thinking of using a PC or other steam generator. Then run the steam to a nipple on one end of the tube and have an outlet nipple on the other end.

It would basically be rr's 55gal steam barrel except smaller diameter.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: maddchef]
    #21763001 - 06/04/15 07:49 PM (8 years, 7 months ago)

I just hate the mess of a water bath


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Re: Straw steam sterilization [Re: maddchef]
    #21763044 - 06/04/15 08:02 PM (8 years, 7 months ago)

Doesn't matter how tight you pack it you won't pack it so tight steam won't penetrate if you do it by hand without a weight. it's probably not enough time theoretically but I bet it would be sufficient


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: bodhisatta]
    #21763095 - 06/04/15 08:12 PM (8 years, 7 months ago)

Do you get the jest of my design? I'd be happy to hear suggestions on how to improve it.

I have a couple of outdoor burners but as stated above I'd be afraid to try to make a pressure vessel out of it.

I was inspired by Azures build and I assume a good prefabricated 10in pipe could easily hold 15psi but the lid would be what stops me.

As I don't weld myself I can easily find someone to seal one end but the lid is where I would bust.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

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Re: Straw steam sterilization [Re: maddchef]
    #21763111 - 06/04/15 08:16 PM (8 years, 7 months ago)

just take a 55 gal drum and make a trivet for the bottom and load it full of bags fill it to the trivet with water and make a lid.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: bodhisatta]
    #21763200 - 06/04/15 08:36 PM (8 years, 7 months ago)

So just make a 55gal steamer and set it on a burner? No pressure or nipples involved lol

Guess if I did that I could fit 5 "logs" at a time on end.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Edited by maddchef (06/04/15 08:37 PM)


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Re: Straw steam sterilization [Re: maddchef]
    #21763215 - 06/04/15 08:39 PM (8 years, 7 months ago)

gigantic PF tek pot and lid basically. if at first you don't succeed up your time.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: bodhisatta]
    #21763256 - 06/04/15 08:45 PM (8 years, 7 months ago)

I wonder what the safe psi of new 55gal drums is rated? I'm sure its not very high. Maybe I just need to save the time and effort and try to follow Azures path and build an actual PC.

Probably save myself many many hours a month with an initial investment.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Re: Straw steam sterilization [Re: maddchef]
    #21763266 - 06/04/15 08:47 PM (8 years, 7 months ago)

55g drum psi rating is probably less than 1 psi but it would hold 1 psi just fine.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: bodhisatta]
    #21763293 - 06/04/15 08:52 PM (8 years, 7 months ago)

1psi might as well be 0psi. Well that's depressing.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Re: Straw steam sterilization [Re: maddchef]
    #21763360 - 06/04/15 09:07 PM (8 years, 7 months ago)

This might be of interest.  I have been playing around with invitro mini straw subs with decent results.  I am still tweaking things but the gist is flour suspended in water,  then used to hydrate the straw. Straw and flour are then packed into containers in this case large pp5 ziplocs, and sterilized. I usually use wheat flour so I sterilize 90 min 15 psi but I did do one run with brf and got away with 70 min. These are then inoculated with LC or LI. BE was pretty good and I have yet to try them with a really good clone. Dry weight of materials for these is usually around 70 grams each.

A few pics. . .



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Re: Straw steam sterilization [Re: Pastywhyte]
    #21763375 - 06/04/15 09:12 PM (8 years, 7 months ago)

Without the flour do you think LI would jump off directly to straw?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: maddchef]
    #21763381 - 06/04/15 09:13 PM (8 years, 7 months ago)

Hell I just need a 5ft tall PC lol.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Re: Straw steam sterilization [Re: maddchef]
    #21763386 - 06/04/15 09:14 PM (8 years, 7 months ago)

Hard to say. Yields would certainly suffer. I wanna say it would colonize but I'm not sure I would be worth it in the end.


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Re: Straw steam sterilization [Re: Pastywhyte]
    #21763398 - 06/04/15 09:17 PM (8 years, 7 months ago)

Well, I can't recall seeing a grow, but I believe you bodhi. I must have missed that.

Regardless, I still think it'd be easier and more efficient to pasteurize. I also fucked up when I said 120 minutes. I was thinking 15 PSI for some reason. I'd steam for a full 8 hours if I were trying to sterilize straw.

BTW people who aren't experienced (or aren't posting or don't have an account or whatever), this is a good experiment. Don't get all crazy, tweak things a little at a time. We're out to make things easier, and I could see how this could make things easier in some situations.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: 36fuckin5]
    #21763409 - 06/04/15 09:19 PM (8 years, 7 months ago)

Yes please at least learn actual proper pasteurization for your own sake.

I couldn't really justify 8 hours for 5 straw logs.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Re: Straw steam sterilization [Re: 36fuckin5]
    #21763430 - 06/04/15 09:22 PM (8 years, 7 months ago)

what is easier anyway. doing is doing. I think it would be cool to see a result. people are concerned with how someone else's grow will turn out. I could see if it was asking how to get started and this idea was pitched.

I used sterilized straw instead of coir in a muda bottle tek. so it's straw with other things like grain and vermiculite. but still it's sterilized straw.

how sterilized the straw needs to be is up to guess work or empirical study. I say figure it out if you're interested.


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Re: Straw steam sterilization [Re: bodhisatta]
    #21763533 - 06/04/15 09:45 PM (8 years, 7 months ago)

Too bad there's no a way to build the log and then run a steam line directly into one end of it. That would save a good deal of time.

Be all nasty and waterlogged with likely no way to drain it off too lol.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblemush madness
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Re: Straw steam sterilization [Re: maddchef]
    #21763561 - 06/04/15 09:50 PM (8 years, 7 months ago)

Could you use 12 inch pvc with screw on caps, put a 1 inch hole in one cap ( for steam exhaust) and plumb the steam into the other end?


Edited by mush madness (06/04/15 09:51 PM)


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Re: Straw steam sterilization [Re: mush madness]
    #21763615 - 06/04/15 10:02 PM (8 years, 7 months ago)

Have to use the high temp PVC, can't remember what its called though. That's actually the exact design I had in mind.

The only way I could see this being worth my time, effort, and money is if I did somewhat of a modular system and created 8-10 of the tubes.

Downside to that is I'd likely have to use a very large or powerful steam generator and would burn through a dickload of fuel to run it.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Re: Straw steam sterilization [Re: maddchef]
    #21763647 - 06/04/15 10:07 PM (8 years, 7 months ago)

55 gallon banded drum and wood fire a possibility for you?


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Re: Straw steam sterilization [Re: mush madness]
    #21763703 - 06/04/15 10:20 PM (8 years, 7 months ago)

Wood or my propane burners. I prefer propane since its easier to regulate.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Re: Straw steam sterilization [Re: maddchef]
    #21763744 - 06/04/15 10:28 PM (8 years, 7 months ago)

I asked about wood due to its cheap heat, I dont think you would have to regulate the heat to much its not like you are building pressure. And 35 to 40 gallons of water will go a long way


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: mush madness]
    #21763783 - 06/04/15 10:37 PM (8 years, 7 months ago)

35-40 gallons will take an eon to heat


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblemush madness
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Re: Straw steam sterilization [Re: maddchef]
    #21763814 - 06/04/15 10:47 PM (8 years, 7 months ago)

1.5 hours or so with a hot fire but it will run the 8 hours that you need


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Re: Straw steam sterilization [Re: mush madness]
    #21763833 - 06/04/15 10:53 PM (8 years, 7 months ago)

I can fit 12 of those pp5's in my sterilizer at a time. I usually put 10 in my 66 quart monos dialed in as normal. Its a good enough yield and I am finding with straw subs with ms I am seeing around 150-200% BE first flush and close to 100% second. I'm sure a good clone will blow that out of the water. Straw that is colonized in vitro seems far more contam resistant as well compared to spawning to it.

Bulk bottles also really kick ass and I can get really good yields from them as well. Muda's tek is something i highly recommend. It even works with grain inoculate instead of liquids. My wikidzon clone was pumping out 20-25 dry grams a bottle. That's with less than a pint of grain. I can jam 13-15 of them into a 66 quart mono. So with that I was getting around 10-11 dry oz first flush in the same space as a regular mono. But it was far more contam resistant amd the second flushes were decent.



Point is that invitro bulk may not be as large a sub as people are used to. But pack a mono with enough of them and it operates the same way, your yield is great and its very contam resistant. Its also less work. One heat treatment, inoculate and then when its done remove the lid and shake on some verm. Done.



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Offlinecilasiah
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Re: Straw steam sterilization [Re: Pastywhyte]
    #24964236 - 02/03/18 03:40 PM (5 years, 11 months ago)

wow! really really nice fruited jars and yields!


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