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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Straw steam sterilization [Re: maddchef]
#21761748 - 06/04/15 01:39 PM (8 years, 7 months ago) |
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Couldn't hurt.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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GreenRabbit
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Re: Straw steam sterilization [Re: 36fuckin5]
#21762220 - 06/04/15 04:00 PM (8 years, 7 months ago) |
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Quote:
36fuckin5 said: I'd do it for at least 2 hours. Straw is dirty stuff.
I noticed a increase in success rates for steam sterilizing BRF jars when going from 90 minutes to 120. You can steam sterilize for as long as you want, I remember RR saying something like 2 hours at 15psi is equivalent to 8 hours steam sterilization. Don't quote me on that though...
I still don't have 100% success at 2 hours with BRF jars so I'd recommend at least 2.5 to 3 hours.
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maddchef
Vaginal escape artist



Registered: 09/04/09
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Re: Straw steam sterilization [Re: GreenRabbit]
#21762230 - 06/04/15 04:05 PM (8 years, 7 months ago) |
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True about the 8 hours but that is for grain.
Straw should be a bit easier since it is pretty hollow and won't be dense in my use case.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Straw steam sterilization [Re: maddchef]
#21762271 - 06/04/15 04:25 PM (8 years, 7 months ago) |
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Try a few of each.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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mushpunx
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Re: Straw steam sterilization [Re: 36fuckin5]
#21762496 - 06/04/15 05:34 PM (8 years, 7 months ago) |
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I get what youre trying to do and its an interesting Idea, like transfering your grains into a bag of sterile straw and letting it colonize invitro
For pasturizing straw Ive found the buckets (like you would use to prep coir) work perfect I just soak my straw in some soapy water overnight and then strain, heat my water to around 180 and pour it in and then cap. After an hour the end temp is usually close to 150 so it works out pretty well for doing smaller batches
--------------------
 Amateur Mycologists United AMU Q&A
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
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Re: Straw steam sterilization [Re: mushpunx]
#21762674 - 06/04/15 06:34 PM (8 years, 7 months ago) |
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Quote:
maddchef said: Would a soak in bleach or lime adjusted water help to kill anything off before sterilization or does that only serve to make it inhabitable for spore germination?
Don't use bleach. Bleach only kills 99.9%. A single sneeze droplet contains over.... well... I've got a comic for you.

I don't care what anyone else here says. Endospores are still endospores. If your goal is sterilization then you NEED to sterilize it. This isn't something that changes just because you're using a less dense medium. If you want 100% success you should do the 8 hour steaming. If you only partially sterilize your straw you're setting yourself up for contamination.
And yes residual bleach will affect mycelium unless you can figure out a way to wash it out of the straw later. Even better though, do some jars at 2 hours, some at 4 hours and some at 8 hours and see which one gets better results.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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The bleach isn't used was a sterilizer in this instance, its used for a major pH swing. Endospores are endospores indeed but the density of your chosen substrate will determine how quickly center mass reaches temp.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: Straw steam sterilization [Re: 36fuckin5]
#21762887 - 06/04/15 07:21 PM (8 years, 7 months ago) |
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Quote:
36fuckin5 said: Many people have tried sterilized straw, all have failed.
I've done it a few times 
if it was just straw though I would say at least 60m. depends on how sterilized you want it. what the original contaminant load was too.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
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Re: Straw steam sterilization [Re: bodhisatta]
#21762987 - 06/04/15 07:46 PM (8 years, 7 months ago) |
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Might as well divulge why I'm so curious.
I'm wanting to build something I can sterilize straw logs in. Likely a large diameter pipe.
I would use poly tubing longer than what the original log would be and stuff very loosely. Have one end sealed per usual and possibly have the open end treated like a spawn bag folded up with a sleeve in it.
After sterilization open the open end in front of my hood and dump in spawn, then massage until I get grain to the other end. This should be pretty easily done since the straw will be so loose.
Once spawn is distributed then simply seal and squeeze the log like a backwards tube of toothpaste to compact it to normal density and size, then wrap with a zip tie or bailing wire to hold shape.
I am afraid to make a pressure vessel so I was thinking of using a PC or other steam generator. Then run the steam to a nipple on one end of the tube and have an outlet nipple on the other end.
It would basically be rr's 55gal steam barrel except smaller diameter.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
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Re: Straw steam sterilization [Re: maddchef]
#21763001 - 06/04/15 07:49 PM (8 years, 7 months ago) |
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I just hate the mess of a water bath
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: Straw steam sterilization [Re: maddchef]
#21763044 - 06/04/15 08:02 PM (8 years, 7 months ago) |
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Doesn't matter how tight you pack it you won't pack it so tight steam won't penetrate if you do it by hand without a weight. it's probably not enough time theoretically but I bet it would be sufficient
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Straw steam sterilization [Re: bodhisatta]
#21763095 - 06/04/15 08:12 PM (8 years, 7 months ago) |
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Do you get the jest of my design? I'd be happy to hear suggestions on how to improve it.
I have a couple of outdoor burners but as stated above I'd be afraid to try to make a pressure vessel out of it.
I was inspired by Azures build and I assume a good prefabricated 10in pipe could easily hold 15psi but the lid would be what stops me.
As I don't weld myself I can easily find someone to seal one end but the lid is where I would bust.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: Straw steam sterilization [Re: maddchef]
#21763111 - 06/04/15 08:16 PM (8 years, 7 months ago) |
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just take a 55 gal drum and make a trivet for the bottom and load it full of bags fill it to the trivet with water and make a lid.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
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Re: Straw steam sterilization [Re: bodhisatta]
#21763200 - 06/04/15 08:36 PM (8 years, 7 months ago) |
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So just make a 55gal steamer and set it on a burner? No pressure or nipples involved lol
Guess if I did that I could fit 5 "logs" at a time on end.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
Edited by maddchef (06/04/15 08:37 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: Straw steam sterilization [Re: maddchef]
#21763215 - 06/04/15 08:39 PM (8 years, 7 months ago) |
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gigantic PF tek pot and lid basically. if at first you don't succeed up your time.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
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Re: Straw steam sterilization [Re: bodhisatta]
#21763256 - 06/04/15 08:45 PM (8 years, 7 months ago) |
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I wonder what the safe psi of new 55gal drums is rated? I'm sure its not very high. Maybe I just need to save the time and effort and try to follow Azures path and build an actual PC.
Probably save myself many many hours a month with an initial investment.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: Straw steam sterilization [Re: maddchef]
#21763266 - 06/04/15 08:47 PM (8 years, 7 months ago) |
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55g drum psi rating is probably less than 1 psi but it would hold 1 psi just fine.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
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Re: Straw steam sterilization [Re: bodhisatta]
#21763293 - 06/04/15 08:52 PM (8 years, 7 months ago) |
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1psi might as well be 0psi. Well that's depressing.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Straw steam sterilization [Re: maddchef]
#21763360 - 06/04/15 09:07 PM (8 years, 7 months ago) |
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This might be of interest. I have been playing around with invitro mini straw subs with decent results. I am still tweaking things but the gist is flour suspended in water, then used to hydrate the straw. Straw and flour are then packed into containers in this case large pp5 ziplocs, and sterilized. I usually use wheat flour so I sterilize 90 min 15 psi but I did do one run with brf and got away with 70 min. These are then inoculated with LC or LI. BE was pretty good and I have yet to try them with a really good clone. Dry weight of materials for these is usually around 70 grams each.
A few pics. . .
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maddchef
Vaginal escape artist



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Re: Straw steam sterilization [Re: Pastywhyte]
#21763375 - 06/04/15 09:12 PM (8 years, 7 months ago) |
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Without the flour do you think LI would jump off directly to straw?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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