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Invisiblemaddchef
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Straw steam sterilization
    #21759909 - 06/04/15 12:19 AM (8 years, 7 months ago)

For an experiment I will be running in the next few days is anyone able to give me a semi accurate/starting point timetable of straw sterilization?

Say if one were to loosely fill a qt jar and PC it?

Only I am looking for a guestimate of how long you would steam sterilize said jar for? X minutes @ 212f

Hopefully I'm not looking at 8 hrs like grain. I wouldn't think so especially because I said LOOSE.

Thanks
:havesomescience:


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleImperfect Iam
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Re: Straw steam sterilization [Re: maddchef]
    #21759919 - 06/04/15 12:25 AM (8 years, 7 months ago)

You don't want to sterilize straw, you want to pasteurize it in 140-160 degree water for 1 hour, to an hour and a half.

It is also said it helps straw to retain water by soaking it in an anti bacterial soap before pasteurization.


--------------------
All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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OfflineCharleskun
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Re: Straw steam sterilization [Re: Imperfect Iam]
    #21759959 - 06/04/15 12:44 AM (8 years, 7 months ago)

Quote:

Imperfect Iam said:
You don't want to sterilize straw, you want to pasteurize it in 140-160 degree water for 1 hour, to an hour and a half.

It is also said it helps straw to retain water by soaking it in an anti bacterial soap before pasteurization.




:whathesaid:

Just get like a 5 gallon bucket with a lid and put your straw + boiling water in and close the lid, wait about 2 hours and you should be good.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Charleskun]
    #21760566 - 06/04/15 07:31 AM (8 years, 7 months ago)

I understand well proper straw prep and that's not the info I'm after.

I'm asking for my own reasons if you were going to steam sterilize a quart of loose straw how long you would run it for?

I own several PCs, modified trashcan for pasteurization, hood, among other things so I'm not trying to take noob shortcuts.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMyconin
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Re: Straw steam sterilization [Re: maddchef]
    #21760573 - 06/04/15 07:35 AM (8 years, 7 months ago)

If you really want to sterilize it for curiosity's sake, I say err on the side of caution. Go for a 2hr or more run in the PC, although I would guess that the usual 90min run should be ok.

If this is all just an experiment, why not set up a couple different runs for different time periods and see which ones perform optimally?


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Myconin]
    #21760735 - 06/04/15 08:47 AM (8 years, 7 months ago)

It won't be pc'd it will be steam sterilized.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMad Season
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Re: Straw steam sterilization [Re: maddchef]
    #21760741 - 06/04/15 08:49 AM (8 years, 7 months ago)

Pc is steam sterilization. You can't go past 100° with water. It's pressurized steam. You need to specify and say steam sterilization at 1 psi.


--------------------
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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Mad Season]
    #21761094 - 06/04/15 10:35 AM (8 years, 7 months ago)

How hot exactly is steam not under pressure? When we tell ppl how to do the pf tek we say steam sterilize when talking about a pot. We say PC when we mean under pressure


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMad Season
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Re: Straw steam sterilization [Re: maddchef]
    #21761118 - 06/04/15 10:47 AM (8 years, 7 months ago)

I've never taken the time to measure the temperature of steam. I don't know what more to tell you, except that most of us who are up to date refer to steam sterilization as pcing. I call pf tek just boiling jars. It's not really even sterilization, to boil and sterilize you have to do it for 8 hours.

To be specific, if it's not under pressure, it's just steam with no pressure or steam at 1 psi.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Mad Season]
    #21761125 - 06/04/15 10:49 AM (8 years, 7 months ago)

Steam can only get to 212f I believe under no pressure.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
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Re: Straw steam sterilization [Re: maddchef]
    #21761153 - 06/04/15 10:57 AM (8 years, 7 months ago)

Many people have tried sterilized straw, all have failed.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: 36fuckin5]
    #21761392 - 06/04/15 12:06 PM (8 years, 7 months ago)

Understood and agreed. I don't plan on sterilizing it and spawning to it like a tub or stuffing a log.

Sterilized and left in its vessel is: jar, bag, tube, etc. It should behave like any other sterile media and be decently resistant once colonized and "birthed" no?

What I have in mind I plan on colonizing it in the container used to sterilize it. Bag for instance, sterilize a straw bag, open in front of a hood and pour in spawn, reseal.

Would this not work in theory?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
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Re: Straw steam sterilization [Re: maddchef]
    #21761405 - 06/04/15 12:09 PM (8 years, 7 months ago)

I suppose that'd work, but it'd be easier to just dump some hot water over it and throw in some lime. Plus you could do big-ass logs or a basket that way.

You may have problems once you introduce FAE though. It'd definitely be a 1-and-done thing.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineGreenRabbit
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Re: Straw steam sterilization [Re: Mad Season]
    #21761493 - 06/04/15 12:33 PM (8 years, 7 months ago)

Quote:

Mad Season said:
Pc is steam sterilization. You can't go past 100° with water. It's pressurized steam. You need to specify and say steam sterilization at 1 psi.




Increasing pressure raises the BP of water, so you can go past 100C. But beyond that, yes, the steam is pressurized as well.

Steam sterilization is not 1 psi. It is 0 psi gage pressure or 1 atm absolute pressure (101 kPa or 14.7 psi).


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InvisibleMad Season
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Re: Straw steam sterilization [Re: GreenRabbit]
    #21761518 - 06/04/15 12:42 PM (8 years, 7 months ago)

Truth. It's also why you can cool an engine with no boiling beyond 100°. However I believe it's like 1 psi ups it by 2 or 3°, and we're relying on the high heat from the steam beyond the 130-140° water to sterilize, thus it's technically steam sterilization. It confused us because that's what we call pcing. It's a lot easier to say steam sterilization at atmospheric pressure. It wouldn't confuse everyone.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Mad Season]
    #21761539 - 06/04/15 12:47 PM (8 years, 7 months ago)

OK so now that all that has been cleared up. If you were to STEAM not PC a quart or bag of loose straw how long would it take to achieve sterilization? 60,90,120, etc.

Best guesses anyone?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMad Season
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Re: Straw steam sterilization [Re: maddchef]
    #21761547 - 06/04/15 12:49 PM (8 years, 7 months ago)

120 or more.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblemaddchef
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Re: Straw steam sterilization [Re: Mad Season]
    #21761724 - 06/04/15 01:30 PM (8 years, 7 months ago)

Really? I was thinking shorter. We PC solid grains for 90 @ 250ish so I figured with straw being hollow and loose that 212f @ 90mins might cut it.

I dunno what kind of bacteria straw harbors though, I only ever see it succumb to mold.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
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Re: Straw steam sterilization [Re: maddchef]
    #21761740 - 06/04/15 01:35 PM (8 years, 7 months ago)

I'd do it for at least 2 hours. Straw is dirty stuff.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
Invisiblemaddchef
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Registered: 09/04/09
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Re: Straw steam sterilization [Re: 36fuckin5]
    #21761743 - 06/04/15 01:37 PM (8 years, 7 months ago)

Would a soak in bleach or lime adjusted water help to kill anything off before sterilization or does that only serve to make it inhabitable for spore germination?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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