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mycologyinfo
Stranger
Registered: 03/16/14
Posts: 174
Last seen: 8 years, 6 months
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Jar colonization questions
#21756466 - 06/03/15 08:10 AM (8 years, 7 months ago) |
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I'm moving at the end of summer, and am wondering if 8-10 weeks is enough to finish a monotub grow. I have MS syringes. Would it be more beneficial to make an LC or grow it out in agar, then inoculate the jars? Would that make the end colonization time faster? Or do I have enough time to do just an MS inoculation. Thanks
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
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Conservative estimation:
2 weeks to get a clean culture on agar 2 weeks to colonize jars 2 weeks to colonize substrate 2 weeks to fruit
I say go for it 
plenty of leeway in that 'conservative' estimation
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mycologyinfo
Stranger
Registered: 03/16/14
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Last seen: 8 years, 6 months
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So by those estimates, would MS jars not colonize within 4 weeks? Also, is the easy agar tek good enough to get clean cultures?
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 57 minutes
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link me to your easy agar tek (they're alot of 'em, and they're all kind'a easy, but I'm sure it'll be fine, as all agar teks include sterilizing)
if you have enough grain and jars, ya might wanna do both, MS some jars and as a back-up do some culture work on agar
my first instinct is to goto agar, it would be less risky than shooting MS solution into some jars and hope'n it was clean 
and if you have the ability to agar, then....why not safety first, right?
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mycologyinfo
Stranger
Registered: 03/16/14
Posts: 174
Last seen: 8 years, 6 months
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http://www.shroomery.org/forums/showflat.php/Number/19208976 there's the link. Point me to a better one if you know one. Also, are there teks for how to get a clean culture for beginners? Like how to do the actual work on agar. And I guess I could inoculate jars then work on agar..worst case is some fail and I'll have cultures to replace it. thanks for help
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 12 hours
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Quote:
MonkeyJesusFresco said: Conservative estimation:
2 weeks to get a clean culture on agar 2 weeks to colonize jars 2 weeks to colonize substrate 2 weeks to fruit
I say go for it 
plenty of leeway in that 'conservative' estimation
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 57 minutes
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yeah man, no problem.
I would definitely check out some...oh wait...
I just clicked on the link you provided and then opened a new tab to search for pastyplates (i.e. the link you provided)
I give that tek a 
The only issue I have is the tape that's used for g/e. Shouldn't be too hard to come across, but I'm out in the middle of goddamn nowhere, so if you can't fine the right tape, you can just pull poly through like this...also, make sure your containers are polypropylene (PP5) and...um...agar work...
well, if you're working from a print, you can take an inoculation loop and swipe it across the print, if using a spore syringe, just a tiny little drop will do ya; I would do multiple plates, and have some more plates made up on stand by for making the transfers.
That's all I can think of off the top of my head, check my sig too I guess...good luck!
-------------------- LAGM v 2.024 - endo cabendo
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mycologyinfo
Stranger
Registered: 03/16/14
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Last seen: 8 years, 6 months
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Got everything I needed. Thanks for the help!
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mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
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you can also use 1 drip from spore syringe onto sterile cotton swab and streak multiple plates
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mycologyinfo
Stranger
Registered: 03/16/14
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Last seen: 8 years, 6 months
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Re: Jar colonization questions [Re: mustangbob3]
#21757180 - 06/03/15 12:12 PM (8 years, 7 months ago) |
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How would you get a sterile swab? Also, am I looking to isolate, or just have a clean culture?
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Re: Jar colonization questions [Re: mustangbob3]
#21757269 - 06/03/15 12:38 PM (8 years, 7 months ago) |
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Quote:
mustangbob3 said: you can also use 1 drip from spore syringe onto sterile cotton swab and streak multiple plates 
One swab per dish. If you had a contam on that one swab, it would then go to each dish of agar you dared to touch it to. You should be much more diligent with sterile technique.
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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mycologyinfo
Stranger
Registered: 03/16/14
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Re: Jar colonization questions [Re: Myconin]
#21757280 - 06/03/15 12:44 PM (8 years, 7 months ago) |
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Ah. Well thank you.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
mycologyinfo said: How would you get a sterile swab? Also, am I looking to isolate, or just have a clean culture?
you can PC a q-tip. I do it in jars. or you can just flame your needle and squirt spore solution on it and swipe it on the plate, keeps the excess water out.
the iso/clean is up to you, I recommend just cleaning as ISOs are a mystery until you fruit them and very few will be worthy of keeping around!
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mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
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Re: Jar colonization questions [Re: Myconin]
#21757341 - 06/03/15 01:03 PM (8 years, 7 months ago) |
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Quote:
Myconin said:
Quote:
mustangbob3 said: you can also use 1 drip from spore syringe onto sterile cotton swab and streak multiple plates 
One swab per dish. If you had a contam on that one swab, it would then go to each dish of agar you dared to touch it to. You should be much more diligent with sterile technique.
it your plates are sterile and if the swab is sterile the only source of contamination was the spores on the print or the air in sab to which you will be exposed with any amount of swabs! to which you would then have to clean up anyway even if u use several swabs one touch of the unsterile print and its there. and the point of agar is to clean up/garantee a culture or be sure of a clean culture. the contam if any on the swab from the print matters not thats why your using agar in the first place so you can select away from any contams amoungst the spore on a print
using the same swab allow you to spread the spore mass like you would when you streak a plate and at the end of the streak its easier to seperate smaller colonies to further isolate as they have more space between. allow more transfers of different colonys.
thus allowing more chance to find a good one and a clean culture
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Re: Jar colonization questions *DELETED* [Re: mustangbob3]
#21757373 - 06/03/15 01:10 PM (8 years, 7 months ago) |
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Post deleted by MyconinReason for deletion: This was posted before Bob had the whistle blown on him.
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 57 minutes
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Re: Jar colonization questions [Re: Myconin]
#21757439 - 06/03/15 01:30 PM (8 years, 7 months ago) |
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mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
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OP ignore him with agar you want lots of transfers at each stage purly for back up as some may not be cleaned fully at the transfer and also when you get to an isolate theres no way to know if it will even fruit before you try it. dont do this.. have back ups so you have plenty to test to find that 1 good culture amongst the bad ones.
Quote:
Which is why you "should be more diligent with sterile techniques". And also, if you can get your work done cleaner, with less agar plates = efficiency.
Honestly dude, do you even grow? Or use spell check for that matter. I agree with GR, you sound like your a 12 year old trying to sound 30. It's not working out well for you, and our intelligent members can see that quite clearly as you've been previously told.
You should listen to your own quote, you sound like you admire yourself a little too much.
tbh you can be as sterile as you like and you still cant guarantee that print is clean this has nothing to do with experience or the way print was taken. even working in a still air box you are exposing the swabs to unsterilised air!! you cant get around that how would you recommend being more sterile? if everything used is already sterile? the print is the weak link that you cannot control not the swab this is why so many people fear using MS syringes and learn agar!
simply with unclean spores and agar more plates and more transfers of individual colonies give more chance of a clean culture not to mention more cultures to test out to find a decent 1 
with agar you will use lots of plates just to find that 1 culture most will be tossed out! but this is not as inefficient as it seems.. imagine isolating 1 clean culture and then making lots of spawn with it and then monos ect just to find its shit and have to start again. months wasted and lots of supplies. make your mistakes on agar it will save stress later and time and will make you more efficient!
you can isolate 1 clean culture- but that dosent mean its a good fruiter or a fruiter at all lol. numbers work in the mycologists favour sorry thats just how i see it With all agar work its guessing until you fruit your chosen isolates to see how they preform, and to limit ones self to 1 culture is a risky business
i would advocate transferring several different pieces from each plate to its own new plate at every stage!! better to be safe than sorry. the only shortcut you want to be taking is using a clone to agar instead of spores to swing the odds in your favour of a fruiting culture, this way and only this way you wont need as many cultures to test
if the print is dirty then it matters not how many sterile swabs you use as each time you touch the print it is no longer sterile long before any contact with the agar. using more swabs only will result in more contaminated swabs. 1 or 10 it makes no diff it the print is not clean with 1 swab it only has 1 chance to touch contam on the print... lots swabs all touch different places on the print so more times touching and more areas on the dirty print = more chances to pick up a contam. i touch with 1 swab streaked over several plates as to spread out the spore mass will make it easier and more efficient everytime
i would prefer more to test so i didnt do the isolation work just to find something i wasnt looking for and having to start from scratch!!
its not immature to do that and in the long run is more efficient.
EDIT:why are you attacking me again in a thread for spelling!! stop following me round and bitching at me. do you want this thread closed too? if you dont like what i say fine but at least pull me down for something real. you know following me round just to bitch at what i say because of a simple spelling error is TROLLING and the behaviour of a minor and decent adult could overlook such small things and not ruin the atmosphere of yet another thread imo your childish for keep finding fault with my spelling when it is only minor and changes nothing of what im saying clearly u just want an argument. yes i sometime miss letters and comma's big deal
thats why im being a grass and mods been notified.
EDIT: like how after i responded you just deleted that to try make me look the dick. i should have QFT you. you snake.
for everyone else myconin said the basis of him attacking me again was..
Quote:
because: spellcheck
guess what myconin you cant cover your tracks i quoted you at the start so you couldnt do this 
everyone can see what you said and how you try to troll and stir shit again deleting your posts dosent hide the fact your wrong.
Edited by mustangbob3 (06/04/15 03:22 AM)
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oontribe

Registered: 01/14/15
Posts: 3,570
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Re: Jar colonization questions [Re: mustangbob3]
#21760145 - 06/04/15 02:58 AM (8 years, 7 months ago) |
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What about your 2nd and maybe 3rd flush?
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Re: Jar colonization questions [Re: mustangbob3]
#21760194 - 06/04/15 03:32 AM (8 years, 7 months ago) |
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Quote:
mustangbob3 said: OP ignore him with agar you want lots of transfers at each stage purly for back up as some may not be cleaned fully at the transfer and also when you get to an isolate theres no way to know if it will even fruit before you try it. dont do this.. have back ups so you have plenty to test to find that 1 good culture amongst the bad ones.
Quote:
Which is why you "should be more diligent with sterile techniques". And also, if you can get your work done cleaner, with less agar plates = efficiency.
Honestly dude, do you even grow? Or use spell check for that matter. I agree with GR, you sound like your a 12 year old trying to sound 30. It's not working out well for you, and our intelligent members can see that quite clearly as you've been previously told.
You should listen to your own quote, you sound like you admire yourself a little too much.
tbh you can be as sterile as you like and you still cant guarantee that print is clean this has nothing to do with experience or the way print was taken. even working in a still air box you are exposing the swabs to unsterilised air!! you cant get around that how would you recommend being more sterile? if everything used is already sterile? the print is the weak link that you cannot control not the swab this is why so many people fear using MS syringes and learn agar!
simply with unclean spores and agar more plates and more transfers of individual colonies give more chance of a clean culture not to mention more cultures to test out to find a decent 1 
with agar you will use lots of plates just to find that 1 culture most will be tossed out! but this is not as inefficient as it seems.. imagine isolating 1 clean culture and then making lots of spawn with it and then monos ect just to find its shit and have to start again. months wasted and lots of supplies. make your mistakes on agar it will save stress later and time and will make you more efficient!
you can isolate 1 clean culture- but that dosent mean its a good fruiter or a fruiter at all lol. numbers work in the mycologists favour sorry thats just how i see it With all agar work its guessing until you fruit your chosen isolates to see how they preform, and to limit ones self to 1 culture is a risky business
i would advocate transferring several different pieces from each plate to its own new plate at every stage!! better to be safe than sorry. the only shortcut you want to be taking is using a clone to agar instead of spores to swing the odds in your favour of a fruiting culture, this way and only this way you wont need as many cultures to test
if the print is dirty then it matters not how many sterile swabs you use as each time you touch the print it is no longer sterile long before any contact with the agar. using more swabs only will result in more contaminated swabs. 1 or 10 it makes no diff it the print is not clean with 1 swab it only has 1 chance to touch contam on the print... lots swabs all touch different places on the print so more times touching and more areas on the dirty print = more chances to pick up a contam. i touch with 1 swab streaked over several plates as to spread out the spore mass will make it easier and more efficient everytime
i would prefer more to test so i didnt do the isolation work just to find something i wasnt looking for and having to start from scratch!!
its not immature to do that and in the long run is more efficient.
EDIT:why are you attacking me again in a thread for spelling!! stop following me round and bitching at me. do you want this thread closed too? if you dont like what i say fine but at least pull me down for something real. you know following me round just to bitch at what i say because of a simple spelling error is TROLLING and the behaviour of a minor and decent adult could overlook such small things and not ruin the atmosphere of yet another thread imo your childish for keep finding fault with my spelling when it is only minor and changes nothing of what im saying clearly u just want an argument. yes i sometime miss letters and comma's big deal
thats why im being a grass and mods been notified.
EDIT: like how after i responded you just deleted that to try make me look the dick. i should have QFT you. you snake.
for everyone else myconin said the basis of him attacking me again was..
Quote:
because: spellcheck
guess what myconin you cant cover your tracks i quoted you at the start so you couldnt do this 
everyone can see what you said and how you try to troll and stir shit again deleting your posts dosent hide the fact your wrong.
I removed them because they were not conducive to a healthy forum. It was an outburst of frustration, one that won't happen again to you, I promise. And I'm not hiding, you can't hide anything from the Mods if they want to find it. I said what I said and will face any consequences of my words.
But enough thread jacking. Sorry OP.
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 23 hours
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Re: Jar colonization questions [Re: Myconin]
#21760199 - 06/04/15 03:34 AM (8 years, 7 months ago) |
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One more jacked thread from you guys bickering and I will ban u both.
If it can remain civil, and on topic, then I am all for it.
This adolescence ends here.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
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Re: Jar colonization questions [Re: PussyFart]
#21760220 - 06/04/15 03:47 AM (8 years, 7 months ago) |
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sorry OP If you feel i was jacking your shit, i thought you actually wanted advice on agar and isolation ect
pf i never thread jacked i gave advice relevant to what the op wanted then got attacked for it!
how is that me thread jacking
and the advice was relevant and correct and civil! what your saying to me basicaly is dont ever post
and yes op swabs are great as it limits the amount of water left in the petri. dripping a syringe its easy to push to hard and use to much. less is more with spores
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Edited by mustangbob3 (06/04/15 04:29 AM)
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