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North Spore Shop: Injection Grain Bag

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OfflineGrimfarce
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Registered: 05/19/11
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Fruit Fly Maggots in my agar plate.
    #21746254 - 05/31/15 08:43 PM (8 years, 7 months ago)

There is a lot of condensation in my incubation chamber, perhaps that is why I found a bunch of maggots on my agar plate?  There is condensation inside the agar plate which is normal after sterilizing however the condensation on this one is from the incubation chamber as it was a pre-sterilized dish with no initial condensation.  How can i prevent this?  Should I seal my plate?  Or is it just my incubation chamber that is causing it.  Also, I threw the agar plate out already so no picture.


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InvisibleMad Season
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Re: Fruit Fly Maggots in my agar plate. [Re: Grimfarce]
    #21746407 - 05/31/15 09:22 PM (8 years, 7 months ago)

Try not using an incubation chamber and just do your work in room temperature. People saran wrap/parafilm their dishes as well. I personally just use pasty plates so maggots can't get in. :shrug:


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OfflineOysterKing
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Registered: 02/08/14
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Re: Fruit Fly Maggots in my agar plate. [Re: Grimfarce]
    #21746820 - 05/31/15 11:27 PM (8 years, 7 months ago)

Condensation will occur inside your incubation chamber, or inside your petri dish, if the temperature outside your incubation chamber, or petri dish, is lower than the temperature outside the chamber/dish.

I would guess your problem is temperature fluctuation which is causing condensation.

Check how big a range your thermostat allows. perhaps it is allowing too large a temperature swing.

Quote:

Try not using an incubation chamber and just do your work in room temperature. People saran wrap/parafilm their dishes as well. I personally just use pasty plates so maggots can't get in. :shrug:




:whathesaid: as well. I've never seen a maggot on agar. Got any pics?


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North Spore Shop: Injection Grain Bag


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