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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Bottle of sterile water contaminated!?
    #21745971 - 05/31/15 07:32 PM (8 years, 7 months ago)

I was making a LI a few months back and i had some jars (mason style) of water PCed I don't know exactly how long anymore, atleast 15 minutes.  Probably twice that long but anyhow the lid is solid except for a RTV ship.  It sat on the shelf for atleast 2 months maybe more but I just checked it and there are colonies of black/grey mold everywhere.  It's really amazin because there is no GE and the lid came off with a nice "pop" just now.  The lid was cranked down tightly. 

What does it mean?  Did spores actually survive the PC?  I have no idea...  I may need to run some tests..


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InvisibleMikeBearPig
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Registered: 07/15/14
Posts: 2,319
Re: Bottle of sterile water contaminated!? [Re: invitro]
    #21746360 - 05/31/15 09:10 PM (8 years, 7 months ago)

Check and make sure you are running proper temps during the PC cycle. 

I frequently leave mine out for months with just Micropour tape covering the GE hole and I never have problems with late inoculations.

Just did 2 Yesterday lol


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