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Offlineinvitro

Registered: 05/03/13
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Mold forms floating island on LC
    #21745857 - 05/31/15 07:01 PM (8 years, 7 months ago)

The lc got jostled a bit before the pic, so the surface of the sporulating mold is wet with liquid culture solution.

What kind of mold is this?



I poured out the lc and put one island into a dish.



The pics are a bit out of focus but that's all I can do at the moment.


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InvisibleMad Season
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Re: Mold forms floating island on LC [Re: invitro]
    #21745895 - 05/31/15 07:10 PM (8 years, 7 months ago)

Looks quite trichy to me. :shrug:


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InvisibleMikeBearPig
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Re: Mold forms floating island on LC [Re: Mad Season]
    #21746366 - 05/31/15 09:11 PM (8 years, 7 months ago)



Trich :smile:

My first batch of grain water LC's made some very pretty trich.


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Offlineinvitro

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Re: Mold forms floating island on LC [Re: MikeBearPig]
    #21769222 - 06/06/15 07:35 AM (8 years, 7 months ago)

I keep getting the same exact mold infection over and over and over again. 
IMO In order of likeliness:
These are the possibilities
1) my sfds are not able to keep out the spores (penecillium infection?) or
2) 25 minutes is not enough time to sterilize
3) the rubber seals on the lids are failing somehow.


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InvisibleMad Season
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Re: Mold forms floating island on LC [Re: invitro]
    #21769268 - 06/06/15 07:52 AM (8 years, 7 months ago)

What's your inoculant and do you do it in a sab?

Molds die quick af in the pc. We up the times for bacteria, so it's probably not sterilization. I do 45 minutes with agar and lcs to be safe.

Lots of people use lids with no rubber seals, so I doubt it's that.

If you used cellulose filter discs and kept them wet for a long time, which is highly unlikely, it can get contaminated, but you'd be able to see mold on the disk.

Although there's lots of people who love and hate both agar and lcs, the truth is that I feel they go hand in hand. I feel people should always test lcs on agar, it'll tell you if it's clean way quicker. Also lcs should be inoculated with clean agar, which will increase the success rates 10 fold.


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Edited by Mad Season (06/06/15 07:57 AM)


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InvisibleMikeBearPig
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Re: Mold forms floating island on LC [Re: invitro]
    #21769959 - 06/06/15 10:54 AM (8 years, 7 months ago)

Quote:

invitro said:
I keep getting the same exact mold infection over and over and over again. 
IMO In order of likeliness:
These are the possibilities
1) my sfds are not able to keep out the spores (penecillium infection?) or
2) 25 minutes is not enough time to sterilize
3) the rubber seals on the lids are failing somehow.





Use the regular lids or plastic ones with micropour tape.    It only takes 15min to sterilize LC's made with honey.  I would do grain longer, but I just switched back to honey.  Works 9/10 times with no contams.  I do everything open air.


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Offlineinvitro

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Re: Mold forms floating island on LC [Re: MikeBearPig]
    #21770132 - 06/06/15 11:41 AM (8 years, 7 months ago)

I'm using synthetic filter discs, I'm really guessings it's penicillium spores small enough to get through the pores in the disc.  That's why I wanted the ID on the type of mold.  I'm using a flow hood.

Mike, your using a needle with a ship in open air? 

I don't get the contams early on, I get them late, really late, like weeks after inoculating which leads me to think the sfds are letting those super tiny penicillium spores through.


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InvisibleMikeBearPig
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Re: Mold forms floating island on LC [Re: invitro]
    #21770546 - 06/06/15 01:49 PM (8 years, 7 months ago)

Quote:

invitro said:
I'm using synthetic filter discs, I'm really guessings it's penicillium spores small enough to get through the pores in the disc.  That's why I wanted the ID on the type of mold.  I'm using a flow hood.

Mike, your using a needle with a ship in open air? 

I don't get the contams early on, I get them late, really late, like weeks after inoculating which leads me to think the sfds are letting those super tiny penicillium spores through.





We told you the contam was trich.  I just poke my needle through a layer of MP tape, then cover up with 3 layers of MP tape.  It's the laziest method, I just never have issues.  1/10 will contam if i am careless, which I usually am.


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Offlineinvitro

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Re: Mold forms floating island on LC [Re: MikeBearPig]
    #21770578 - 06/06/15 01:56 PM (8 years, 7 months ago)

My contam looks very distinctively different that yours, I haven't posted enough pics to make that clear yet because I disturbed the colony too much before taking the pic..  Plus lots of people say trich as a catch-all for anything green so I wasn't sure if you really meant trich, apparently you do.


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InvisibleMikeBearPig
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Re: Mold forms floating island on LC [Re: invitro]
    #21770607 - 06/06/15 02:02 PM (8 years, 7 months ago)

Quote:

invitro said:
My contam looks very distinctively different that yours, I haven't posted enough pics to make that clear yet because I disturbed the colony too much before taking the pic..  Plus lots of people say trich as a catch-all for anything green so I wasn't sure if you really meant trich, apparently you do.




Your's might not of been sporalated or as far along as mine , or its the one that comes from breathing .. Forgot the name, starts with an A i think.


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InvisibleMad Season
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Re: Mold forms floating island on LC [Re: MikeBearPig]
    #21770676 - 06/06/15 02:22 PM (8 years, 7 months ago)

Mbp are you talking about Aspergillus? It's in the Trichocomaceae family, same with penicillium.

To accurately identify the exact mold you have, we'd need to see its spores, or at least a clearer pic. You are correct that trich is just what the online mushroom community says when we see POWDERY green mold. It can indeed be trichoderma, penicillium, aspergillus, etc.

It really doesn't matter what it is for our purposes. The filter disks can still keep it out. If you're worried just get new lids and filters, but like I said, work on your inoculant (agars the only way to know 100% it's clean inoculant. I will always test lcs with agar and inoculate lcs with clean agar) and sterile techniques too.


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/06/15 02:23 PM)


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Offlineinvitro

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Re: Mold forms floating island on LC [Re: Mad Season]
    #21770875 - 06/06/15 03:19 PM (8 years, 7 months ago)

Yea I have new filter disks...

I really want to focus in on the species because I want a better grip on it's growth pattern, speed, etc.  So little info floating around about it, especially for lcs.

I might put some to agar for an ID, I'm pretty sure they all show unique morphology on agar (I hope).


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InvisibleMad Season
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Re: Mold forms floating island on LC [Re: invitro]
    #21771509 - 06/06/15 06:59 PM (8 years, 7 months ago)

Understood. It does look different on agar most of the time. Still, like I said, if you had proper techniques, it wouldn't be annoying you.

My house is far from clean and I haven't had to toss anything for a long time. I used to toss every jar and tub until I finally just broke down and used a sab and agar. You still haven't told me what your inoculating your lc with, and if it all came from the same print/syringe


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlineinvitro

Registered: 05/03/13
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Re: Mold forms floating island on LC [Re: Mad Season]
    #21771545 - 06/06/15 07:09 PM (8 years, 7 months ago)

I'm inoculating with a syringe.  My initial contam rate is low, maybe 5% at the most I would say.  But like I said after 3 - 5 weeks, this mold just shows up, even in the jars that show no signs of growth after inoculation.  These sfds I'm using have only been used about twice.   

I'm switching to cellulose filter disks to see how that works.  But I just have a bunch of these jars with sfds that never took off after inoculation, and they are getting contamed, a trich sniper is nailing them one by one.


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