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Offlinenetofbeing
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Registered: 05/31/15
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Why did one of my jars stop colonizing?
    #21745652 - 05/31/15 05:47 PM (8 years, 7 months ago)

About 3 weeks after inoculation I noticed one of my eight jars stopped colonizing. It hasn't seemed to progress at all since I noticed this about two weeks ago.. all of the others are 90-100% and are currently sitting for consolidation. I dont' see any sign of contamination on any of them. They are sitting on a shelf in my closet with the temps being approximately 70f.

Here is a photo for reference:



I was thinking, perhaps it is because during the filling of the jars with substrate I ran out of substrate and had to make a small amount for the one jar that was left - perhaps this was the jar. But I still can't figure out why it hasn't progressed with colonizing.


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Invisiblermshroom
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Re: Why did one of my jars stop colonizing? [Re: netofbeing]
    #21745711 - 05/31/15 06:12 PM (8 years, 7 months ago)

it could have drank all the water out of the substrate or there is no flour or nutrients in that patch of the substrate due to poor mixing. Just give it another week three weeks is good time.

Congrats, those jars look great!


--------------------


Edited by rmshroom (05/31/15 06:14 PM)


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OfflineDensePlacebo
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Re: Why did one of my jars stop colonizing? [Re: rmshroom]
    #21745788 - 05/31/15 06:41 PM (8 years, 7 months ago)

If you have an empty syringe you can try injecting sterile water into that patch.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!




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Offlinephilsfan
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Re: Why did one of my jars stop colonizing? [Re: DensePlacebo]
    #21745840 - 05/31/15 06:58 PM (8 years, 7 months ago)

Could be bacterial contamination as well


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OfflineElement1
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Re: Why did one of my jars stop colonizing? [Re: philsfan]
    #21745937 - 05/31/15 07:21 PM (8 years, 7 months ago)

i have somethign similar happen to me. i have a feeling mine was due to endospore contamination because i didnt steam my jars enough ( i have no pressure cooker) or that it might have been too mich rice :shrug:


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Offlinenetofbeing
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Re: Why did one of my jars stop colonizing? [Re: Element1]
    #21746894 - 05/31/15 11:56 PM (8 years, 7 months ago)

Unfortunately i don't have any empty syringes :/ what are the signs a bacterial contamination?

Also, side note, is condensation on the side of the jars inside normal? And a small amount of water on the bottom of the jar?


Edited by netofbeing (05/31/15 11:58 PM)


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InvisibleMyconin
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Re: Why did one of my jars stop colonizing? [Re: netofbeing]
    #21746960 - 06/01/15 12:28 AM (8 years, 7 months ago)

With this hobby, there are myriad reasons why it could have happened. Bad genetics in spores, contamination, fluctuating temps, poor GE, so on, so forth. Key thing is patience. Wait a little longer for it to colonize more, and smell it before spawning. If it smells off, chuck it. At least you've got 9 more beautiful jars :thumbup:


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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OfflineDensePlacebo
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Re: Why did one of my jars stop colonizing? [Re: netofbeing]
    #21747076 - 06/01/15 01:25 AM (8 years, 7 months ago)

I would say a little condensation is fine my jars always have a little. But if it has condensation I would also say it definitely didn't dry up. So your probably back a some sort of bacteria.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!




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InvisibleMyconin
Mushroom Ninja
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Registered: 05/11/15
Posts: 308
Loc: The shadows... Flag
Re: Why did one of my jars stop colonizing? [Re: DensePlacebo]
    #21747081 - 06/01/15 01:27 AM (8 years, 7 months ago)

Derp, 7 jars. I need sleep lol!


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Offline350shroomz
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Re: Why did one of my jars stop colonizing? [Re: Myconin]
    #21747167 - 06/01/15 02:07 AM (8 years, 7 months ago)

When my jars began to cease in colonizing, I sprayed a little must of distiller water inside the jars, the mycelium's growth grew exponentially. I know some people who just dump about a 1/4 of distiller water in the jars when it becomes dry. They check it in a week and the mycelium already doubled in size, and the extra water was absent.


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OfflinePussyFart
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Re: Why did one of my jars stop colonizing? [Re: 350shroomz] * 1
    #21747175 - 06/01/15 02:11 AM (8 years, 7 months ago)

Quote:

350shroomz said:
When my jars began to cease in colonizing, I sprayed a little must of distiller water inside the jars, the mycelium's growth grew exponentially. I know some people who just dump about a 1/4 of distiller water in the jars when it becomes dry. They check it in a week and the mycelium already doubled in size, and the extra water was absent.



Absolutely Terrible advice........Never open a colonizing jar...and if you do never add unsterilized water.......and distilled water is only good for making spore syringes, as it is a solvent....please stop giving advice here.


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Offlinespacechildo
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Re: Why did one of my jars stop colonizing? [Re: netofbeing]
    #21747415 - 06/01/15 05:42 AM (8 years, 7 months ago)

Quote:

netofbeing said:
Unfortunately i don't have any empty syringes :/ what are the signs a bacterial contamination?

Also, side note, is condensation on the side of the jars inside normal? And a small amount of water on the bottom of the jar?




take a close up pic of the stalled jar and I bet we can show you what bacteria looks like.


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Offlinenetofbeing
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Re: Why did one of my jars stop colonizing? [Re: spacechildo]
    #21758640 - 06/03/15 06:44 PM (8 years, 7 months ago)

Quote:

spacechildo said:
Quote:

netofbeing said:
Unfortunately i don't have any empty syringes :/ what are the signs a bacterial contamination?

Also, side note, is condensation on the side of the jars inside normal? And a small amount of water on the bottom of the jar?




take a close up pic of the stalled jar and I bet we can show you what bacteria looks like.




Here are some pics... sorry for the quality I took em with my phone.





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Invisiblermshroom
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Re: Why did one of my jars stop colonizing? [Re: netofbeing]
    #21758718 - 06/03/15 07:07 PM (8 years, 7 months ago)

Those look like there doing really well I don't see any bacteria in ther keep up the good work


--------------------


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InvisibleMad Season
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Re: Why did one of my jars stop colonizing? [Re: rmshroom]
    #21758726 - 06/03/15 07:09 PM (8 years, 7 months ago)

It doesn't look all that stalled to me. It probably just slowed down. It happens


--------------------
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Offlinenetofbeing
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Re: Why did one of my jars stop colonizing? [Re: Mad Season]
    #21759222 - 06/03/15 09:05 PM (8 years, 7 months ago)

Quote:

rmshroom said:
Those look like there doing really well I don't see any bacteria in ther keep up the good work



Quote:

Mad Season said:
It doesn't look all that stalled to me. It probably just slowed down. It happens




This jar hasn't gotten past what you see in the photo, its been like that for approximately 2 weeks. The other 7 jars will be ready for fruiting next week, whilst this one just remains the same.. you guys think I should just wait?


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OfflineBkillsU
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Re: Why did one of my jars stop colonizing? [Re: netofbeing]
    #21759236 - 06/03/15 09:08 PM (8 years, 7 months ago)

I say isolate it and wait. If nothing changes in a week throw it away. 1/8 jars lost is great compared to my usual 15/40 lost.


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InvisibleMad Season
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Re: Why did one of my jars stop colonizing? [Re: netofbeing]
    #21759237 - 06/03/15 09:09 PM (8 years, 7 months ago)

Yeah. Give it more time. Take a marker and draw the outline of the mycelium. If it grows past it, it's not stalled and is just taking its time. It's probably from a bad moisture content or something.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleMyconin
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Re: Why did one of my jars stop colonizing? [Re: Mad Season]
    #21759584 - 06/03/15 10:36 PM (8 years, 7 months ago)

Quote:

Mad Season said:
Yeah. Give it more time. Take a marker and draw the outline of the mycelium. If it grows past it, it's not stalled and is just taking its time. It's probably from a bad moisture content or something.




:whathesaid: I do that for all my jars every few days to monitor progress. It's a great idea.


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Invisiblermshroom
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Re: Why did one of my jars stop colonizing? [Re: Myconin]
    #21760033 - 06/04/15 01:39 AM (8 years, 7 months ago)

Try raising your incubation temperature 5 degrees


Edited by rmshroom (06/04/15 01:41 AM)


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InvisibleMyconin
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Re: Why did one of my jars stop colonizing? [Re: rmshroom]
    #21760044 - 06/04/15 01:48 AM (8 years, 7 months ago)

Quote:

rmshroom said:
Try raising your incubation temperature 5 degrees




I don't really think temps are the issue imo. 65F to 75F is the golden zone for colonizing temps, and he says he's at 70F. I think at this point, it's in the mycelium's hands now. Wait and see :wink:


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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Offlinespacechildo
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Re: Why did one of my jars stop colonizing? [Re: Myconin]
    #21760445 - 06/04/15 06:34 AM (8 years, 7 months ago)

that growth look pretty thick and bacterial,
if its looked like this for 2 weeks its pretty much toast IMO..


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Invisiblermshroom
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Re: Why did one of my jars stop colonizing? [Re: spacechildo]
    #21765408 - 06/05/15 10:48 AM (8 years, 7 months ago)

Quote:

spacechildo said:
that growth look pretty thick and bacterial,
if its looked like this for 2 weeks its pretty much toast IMO..




I would still try to do something with the cakes, throwing them away when they are so colonized seems like such a waste. and what bacteria do you see? It looks like thick mycelial growth to me. Spacechildo also said my grain jars looked moldy, the same ones that gave me a half pound flush dry and that is no joke. Is everyones jar moldy to you Spacechild or do you not know what bacteria looks like?


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OfflineRyeJar
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Re: Why did one of my jars stop colonizing? [Re: spacechildo]
    #21765431 - 06/05/15 10:56 AM (8 years, 7 months ago)

Quote:

spacechildo said:
if its looked like this for 2 weeks its pretty much toast IMO..




Exactly what I was thinking the entire thread.
mycellium doesn't slow down it only gets faster if everything is right.  If it's looked anything close to that for 2 WEEKS then.....


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InvisibleMad Season
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Re: Why did one of my jars stop colonizing? [Re: RyeJar]
    #21765456 - 06/05/15 11:07 AM (8 years, 7 months ago)

Moisture content is undoubtedly off, genetics just suck, or it's bacteria if it's been 2 weeks. Which is why it's best to mark the mycelium, and see if it's actually stopped completely. Every batch has fast and slow jars. Patience is best.

I'm sure we've all messed up moisture content or something and let a jar keep going even though we thought it'd fail. These guys are quite strong.

This bacterial jar took over 1 month to take over the last little spot enough for me to call colonized:


Spawned using only the bacterial jar
Turned into:


I think I got around an ounce or 2 first flush and it did 2 more semi decent ones.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/05/15 11:10 AM)


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