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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Why???
#21745584 - 05/31/15 05:23 PM (8 years, 7 months ago) |
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so I made 6 jars PF tek style...and inoculated. Only 2 jars have myc growth after 3 weeks.
BUT heres the thing...
Both jars with growth only has growth on 1 of the inoculation points. I have no idea how this could be.
any ideas?
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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I had the same problem with one of my PF tek grows. Is their adequate gas exchange? I made the mistake of leaving the tin foil on my very first set of jars and it colonized slowly and never finished. After trying bulk, I don't think I'm going back to PF tek, I just don't see the need when working with grain is so much easier.
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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I used the same method for all the jars. I followed the "lets grow mushrooms" tek to the T.
I filled the jars with the right ratio of verm/brf..covered with dry verm..then put the lids back on. I PC'd for 90 minutes at 15PSI...I used micropore tape for the GE holes..i did the exact same thing as the video, like I mentioned 
I thought it was my spores until I saw definite growth on 2 of the jars the other day. And only each jar the growth is only on 1 inoculation point.
I had REALLY thought it was the spores because I noc'd up 3 quart jars of grain and they all contaminated.
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Pics? Did you make any improvisations at all during prep?
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Why are you still doing PF tek grows..?
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Quote:
Inocuole said: Why are you still doing PF tek grows..?
Because i like simple. I find pf tek to be the easiest way to get myc for spawning to bulk substrate. I dont fruit the cakes..i break them up and spswn to bulk. I dont like dealing with grains.
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Makes sense. So, did you make any improvisations at all?
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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BRF cakes take soooooong to colonizw compared to grains man! Theres no real practical way to expand your spawn.
Your jars will colonize with just one inoculation point colonizing though
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 Amateur Mycologists United AMU Q&A
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
Bill_Oreilly said: I used the same method for all the jars. I followed the "lets grow mushrooms" tek to the T.
I filled the jars with the right ratio of verm/brf..covered with dry verm..then put the lids back on. I PC'd for 90 minutes at 15PSI...I used micropore tape for the GE holes..i did the exact same thing as the video, like I mentioned 
I thought it was my spores until I saw definite growth on 2 of the jars the other day. And only each jar the growth is only on 1 inoculation point.
I had REALLY thought it was the spores because I noc'd up 3 quart jars of grain and they all contaminated.
if you did everything as in the videos, including wiping the rim of the jar before adding the dry verm layer its pretty obvious that any contamination comes from the syringe.
If you didn't get growth in more than 1/4 holes on your jars you maybe forgot to shake the syringe properly before inoc'ing? even a clear totally see-through syringe has a buttload of spores in them so lack of spores isn't the reason IME.
btw, why dont you like working with grains? if you have a PC it only takes a couple of tries to get the grain prep down, and grains can be shaken to distribute for faster colonization, g2g'd, inoc'd with agar etc.
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Achillita
Back to the basics



Registered: 05/26/14
Posts: 4,565
Last seen: 3 years, 10 days
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Your spore syringe might be bunk, first 3 times I had bought syringes, not a single one grew anything. Did you make the syringes yourself bill?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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That's the thing about spore syringes, and by association, PF tek. Can never be sure what got scraped up with that print.
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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the spores were from SW. Reputable vendor..never had a problem with them.
like i said..im getting growth on 2 jars but only on 1 inoculation point of each of the 2 jars.
i followed the video to the T.
if it were the spores then i wouldn't have any growth at all.
and yes, i did shake the syringe.
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Achillita
Back to the basics



Registered: 05/26/14
Posts: 4,565
Last seen: 3 years, 10 days
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Agar is much easier to work with than spore syringes. But it might be a sort of contam. How long did you let the spores stay in the syringe?
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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About 3 days
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Bill_Oreilly said: if it were the spores then i wouldn't have any growth at all.
What might possibly make you think that's true? A light bacterial contam could distribute itself in such a way that some spores can germinate in certain spots before it's all taken over.
And I know for sure that even SW spores aren't infallible. It would be unwise to describe my experience with them since that would violate some rules here but I assure you I've grown some bacteria from some items with a SW label.
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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That shouldn't have been a problem then, unless they were bad to begin with
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Quote:
Inocuole said:
Quote:
Bill_Oreilly said: if it were the spores then i wouldn't have any growth at all.
What might possibly make you think that's true? A light bacterial contam could distribute itself in such a way that some spores can germinate in certain spots before it's all taken over.
And I know for sure that even SW spores aren't infallible. It would be unwise to describe my experience with them since that would violate some rules here but I assure you I've grown some bacteria from some items with a SW label.
ah ok I didn't know that. thanks. it could be the spores then
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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bill, if you don't feel like working with agar and still want an easy (and faster than PFtek) solution:
buy some whole oats at a feed store for $0.30/lb, inoculate a jar with spores, then do G2G.
-------------------- (Yes, the egg is real)
How to post pics
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Quote:
FriedEgg said: bill, if you don't feel like working with agar and still want an easy (and faster than PFtek) solution:
buy some whole oats at a feed store for $0.30/lb, inoculate a jar with spores, then do G2G.
Quote:
Bill_Oreilly said: I dont like dealing with grains.
LuLz
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Why??? [Re: Juiceh]
#21748598 - 06/01/15 01:45 PM (8 years, 7 months ago) |
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Thats why I suggested whole oats. they're so easy.
-------------------- (Yes, the egg is real)
How to post pics
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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If you are having syringe problems but still want to work with BRF you can clean up your culture on agar, prep your PF jars with a grain lid and no dry verm layer, and drop your wedge on top.
--------------------
 Amateur Mycologists United AMU Q&A
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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It doesn't matter if you do grains or not, what really matters is putting agar between the spores and the substrate. You need a clean inoculant so you can rule that out completely as a contam vector, then you can do cakes or tubs or logs or whatever the fuck you want because just about everything will colonize without issue.
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Quote:
FriedEgg said: bill, if you don't feel like working with agar and still want an easy (and faster than PFtek) solution:
buy some whole oats at a feed store for $0.30/lb, inoculate a jar with spores, then do G2G.
hiw do I prep them? that's why im not a grain user as of now...I don't think ill be able to get the prep down.
Also, G2G is ehh because I need a SAB. I haven't made one yet, so I need to get on it I guess..
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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For oats, you just boil them for 30-40 minutes until the white starchy center turns clear. Vandergreigen had a tek, with pictures, showing the process. It's incredibly easy.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Why??? [Re: Grey]
#21753858 - 06/02/15 05:34 PM (8 years, 7 months ago) |
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There's an oat tek in my signature that I wrote up. There's a few ways to do it, it's really forgiving compared to other grains.
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