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Danimal1q2w3
Stranger

Registered: 02/19/15
Posts: 6
Last seen: 8 years, 7 months
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Black ink looking stuff and black dots on rye seed
#21742666 - 05/30/15 08:56 PM (8 years, 7 months ago) |
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Hey everyone. Recently inoculated some rye seed jars. As I saw on a forum, soaked the seeds for 24 hours in water with a little bit of coffee. Put the seeds in jars and then pressure cooked them at 15 psi for 80 minutes. Waited about a week and a half for mycelium to develop and o check today and notice what looked like little black fish eggs, or little tiny dots of black spots. This is my first time using the rye seed method and upon shaking the jars, it was like an ink explosion and the entire jar was covered in black ink looking stuff. Obviously it's contaminated, but I want to know if anyone has ever encountered this before. And why it would happen. I feel as if i was overly careful with sterilization. Sterilized the bin the jars are sitting in, pressure cooked everything for 20 minutes more than I was supposed to. Sterilized the air and the walls and the ceiling and everything in the room with the bin. Wore sterile gloves whenever handling anything. Any information you guys could share would be greatly appreciated.

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MikeBearPig
Not liked.

Registered: 07/15/14
Posts: 2,319
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Re: Black ink looking stuff and black dots on rye seed [Re: Danimal1q2w3]
#21742736 - 05/30/15 09:13 PM (8 years, 7 months ago) |
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Quote:
Danimal1q2w3 said: Hey everyone. Recently inoculated some rye seed jars. As I saw on a forum, soaked the seeds for 24 hours in water with a little bit of coffee. Put the seeds in jars and then pressure cooked them at 15 psi for 80 minutes. Waited about a week and a half for mycelium to develop and o check today and notice what looked like little black fish eggs, or little tiny dots of black spots. This is my first time using the rye seed method and upon shaking the jars, it was like an ink explosion and the entire jar was covered in black ink looking stuff. Obviously it's contaminated, but I want to know if anyone has ever encountered this before. And why it would happen. I feel as if i was overly careful with sterilization. Sterilized the bin the jars are sitting in, pressure cooked everything for 20 minutes more than I was supposed to. Sterilized the air and the walls and the ceiling and everything in the room with the bin. Wore sterile gloves whenever handling anything. Any information you guys could share would be greatly appreciated.


Do a full 90min pressure cooking run. (it's likely your 80min was enough)
What medium did you use for inoculation?
Edited by MikeBearPig (05/30/15 09:14 PM)
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Danimal1q2w3
Stranger

Registered: 02/19/15
Posts: 6
Last seen: 8 years, 7 months
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Re: Black ink looking stuff and black dots on rye seed [Re: MikeBearPig]
#21742957 - 05/30/15 10:04 PM (8 years, 7 months ago) |
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Spore syringes
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Danimal1q2w3
Stranger

Registered: 02/19/15
Posts: 6
Last seen: 8 years, 7 months
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Re: Black ink looking stuff and black dots on rye seed [Re: MikeBearPig]
#21743801 - 05/31/15 04:39 AM (8 years, 7 months ago) |
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Anyone has this before?
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Danimal1q2w3
Stranger

Registered: 02/19/15
Posts: 6
Last seen: 8 years, 7 months
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Re: Black ink looking stuff and black dots on rye seed [Re: Danimal1q2w3]
#21772677 - 06/07/15 01:41 AM (8 years, 7 months ago) |
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bump
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Black ink looking stuff and black dots on rye seed [Re: Danimal1q2w3]
#21772694 - 06/07/15 02:01 AM (8 years, 7 months ago) |
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I don't know what it is. Bad pics.. why's one jar completely black?
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