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OfflineSyntheticwords
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The Road to 7 mono tubs
    #21736944 - 05/29/15 01:53 PM (8 years, 7 months ago)

Greetings Humans,

I have recently undertaken a project for an assosciate of mine
I will use my powers for good! Never evil...

So here we begin. I will work and update this post through my ventures

I began with 3 syringes

Cambodian
Amazon
Creepers

Using these strains I created
12 jars of liquid culture



With these jars I will make 10 x 10ml syringes
And I plan to test out the LC tonight to see if it's ready for bulk


I plan to use all the syringes to knock up
42 Quart jars of WBS

That's a whopping 7 mono tubs expected for this project


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OfflinePinPornProducer
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21736964 - 05/29/15 02:00 PM (8 years, 7 months ago)

Good luck man, just a head up though, you may want to test those lcs before going balls out with them. They can be very deceiving and bite you right in the ass very quick.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: PinPornProducer]
    #21736979 - 05/29/15 02:03 PM (8 years, 7 months ago)

I plan to test each jar with a brf cake tonight

They are currently in the PC cooling down


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OfflinePinPornProducer
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21736982 - 05/29/15 02:04 PM (8 years, 7 months ago)

:super:


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Invisible36fuckin5
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Registered: 08/11/03
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Re: The Road to 7 mono tubs [Re: PinPornProducer]
    #21736990 - 05/29/15 02:07 PM (8 years, 7 months ago)

If you have spores left, you might as well just knock up a bunch of jars with spores. It only takes a tiny amount of spores per jar, maybe 1/10 of a ml. By the time those BRF cakes are done you would have plenty of spawn.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21737005 - 05/29/15 02:11 PM (8 years, 7 months ago)

Well the brf cakes are just my testers. No need to wait until they are finished

If I see good healthy spread of mycelium, then I will use the LC to knock up
WBS qt jars


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,079
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21737014 - 05/29/15 02:12 PM (8 years, 7 months ago)

No, you still need to wait for them to finish. Mold and bacteria can show up at any time.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinePinPornProducer
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21737025 - 05/29/15 02:14 PM (8 years, 7 months ago)

:whathesaid: and fruit them, mold doesn't show up sometimes until it's introduced to fresh air (fruiting conditions)


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Invisible36fuckin5
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Re: The Road to 7 mono tubs [Re: PinPornProducer]
    #21737038 - 05/29/15 02:16 PM (8 years, 7 months ago)

I would fruit them anyway, cause why waste spawn, but you don't really need to go that far. If they colonize clean then it should work.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: PinPornProducer]
    #21737067 - 05/29/15 02:24 PM (8 years, 7 months ago)

So before I can determine if my LC is healthy I have to wait until cakes are done?
Under my time parameters It may not be viable to wait to fruit the cakes. The real treasure is in the bulk.

Your saying mold and bacteria are harboring inside the mycelium ?
Fruiting conditions won't be introduced until ....the substrate has consolidated?


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OfflinePinPornProducer
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Re: The Road to 7 mono tubs [Re: Syntheticwords] * 1
    #21737092 - 05/29/15 02:29 PM (8 years, 7 months ago)

It's the same as bulk, grains look healthy and clean but once spawned and fruited, if the spawn was bad, 3-4 days later it tams out. That's why we use agar, contams can't hide. Trich mycelium hides very well within mushroom myc. If you don't want to wait until fruits then test it on agar, you will know within a few days to a week if it's clean or not


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InvisiblePastywhyteMDiscord
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Re: The Road to 7 mono tubs [Re: PinPornProducer] * 1
    #21737104 - 05/29/15 02:32 PM (8 years, 7 months ago)

FYI its best to test LC on agar. You can see right away on the 2D media if its clean or not. Its also better to inoculate LC with agar wedges anyway so the two go hand in hand IMO.

Given that its nearly impossible to have a 100% aseptic LC you need to use it as soon as you can after colonization. Agar gives an edge there for sure.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Pastywhyte]
    #21737137 - 05/29/15 02:42 PM (8 years, 7 months ago)

Currently the LC is on its 10th day

And agar would probably take a while to ship here
As a future reference those helps tremendously


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InvisiblePastywhyteMDiscord
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21737196 - 05/29/15 02:54 PM (8 years, 7 months ago)

I buy agar in an asian super market for cheap. Add your own nutes and its done. It can also be found in health food stores.


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Invisible36fuckin5
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21739127 - 05/29/15 10:57 PM (8 years, 7 months ago)

Quote:

Syntheticwords said:
So before I can determine if my LC is healthy I have to wait until cakes are done?
Under my time parameters It may not be viable to wait to fruit the cakes. The real treasure is in the bulk.




Then wait the proper amount of time. Rushing things leads to failure, especially when you're new enough to have to ask these questions.

Trust me, putting shit off 2 weeks is much better than throwing away 7 tubs' worth of shit. Learn from my mistake, not yours.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinePhosCap
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21739203 - 05/29/15 11:13 PM (8 years, 7 months ago)

I remember reading last year or so Frank went balls deep in LC's and then ultimately lost many tubs due to that unknown factor of contam. Then he bypassed and went straight to agar.  I personally never done LC because of what i read. Agar seems the best, easiest route.


--------------------
Psychoactive Fungi of the World        TLs            Franks teks and Tips        Stonesun's..
Spitballs Quick Rye Prep


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Invisible36fuckin5
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Re: The Road to 7 mono tubs [Re: PhosCap]
    #21739218 - 05/29/15 11:17 PM (8 years, 7 months ago)

I've done lots and lots of LC. There's a reason I quit. If it works you have a fuckload of inoculant ready at once. A couple quarts of LC does a LOT of jars or bags. If it doesn't work, or you happen to contaminate it during a transfer, you lose just as much. Not worth it. Methods need to work 100% of the time for 100% of people who take time to do it properly.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21752126 - 06/02/15 10:33 AM (8 years, 7 months ago)

UPDATE:

Brf cakes are showing signs of healthy growth
If I hadn't posted on this forum I would probably
Feel extremely confident

However. Many of you make me question my work
I will heed your warnings and order a pack of petri agar dishes

Pics coming soon


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Invisibleslapphappypill
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21752334 - 06/02/15 11:32 AM (8 years, 7 months ago)

next stop on that road, 100 monotubs :-P

good luck and keep posting pics/questions as needed!


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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OfflineBurberrySpores
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Re: The Road to 7 mono tubs [Re: slapphappypill]
    #21752453 - 06/02/15 12:02 PM (8 years, 7 months ago)

Lol.  I'm working on 20 right now:lookslucrative:


--------------------
I could go crazy... and no one would notice :seeya:


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: BurberrySpores]
    #21752458 - 06/02/15 12:03 PM (8 years, 7 months ago)

well with my given space I'm trying to do some heavy duty stackable tubs


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Invisibleslapphappypill
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21752474 - 06/02/15 12:06 PM (8 years, 7 months ago)

I've got more than that in fruiting at all times ;-)

I tend to do 12 jars at a time, then as my 971 holds 6 full spawn bags, I do 6 bags at a time, and split my tub mixing up into 2 days for 12 tubs at a time. Always keep the next project coming, and you'll be working all week


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: slapphappypill]
    #21765172 - 06/05/15 09:29 AM (8 years, 7 months ago)

Greetings humans

Sorry for lack of pictures. But there's not much to show

Brf cakes are healthy
And I have already begun sterilizing my grains(WBS)

With a 10lb bag of seed I got 12 quart jars worth
I decided to make 3 of them G2G jars

HOWEVER
When I was pcing the first batch of jars my weight did not jiggle and most of the steam was exiting out of the base of the regulator. The weight DID shake but not often. I continued to pc at the first sign of a minor jingle and I waited for pressure to go down

AFTER
Some cool down time I shook the jars and stored them in a regular tote to continue pcing
But this time I had tightend the piece under the lid which was the cause of so much steam exiting under the weight rather then pushing the weight around

I've read that you can pc grains as much as you want
Or at least twice in my case... This next batch the weight is rocking gently at 15psi so I feel better about this batch. The first batch was almost 2+ hours

If anyone thinks it is a bad idea to pc these grains
Let another human know


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Offlinemetaphoric
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21765223 - 06/05/15 09:48 AM (8 years, 7 months ago)

Quote:

Syntheticwords said:
UPDATE:

Brf cakes are showing signs of healthy growth
If I hadn't posted on this forum I would probably
Feel extremely confident

However. Many of you make me question my work
I will heed your warnings and order a pack of petri agar dishes

Pics coming soon



The thing you gotta watch out for on the shroomery is the advice you're getting is generally from perfectionists. Which is great! Because perfection should be everyone's goal. But once you have your LC made waiting on a brf cake to finish an entire cycle is kind of pointless. It's either going to contam, or it isn't. If it does you'd have to throw away all the LC anyway so the only thing your really wasting is the WBS you inoculated, which is cheap so who cares? Then if it doesn't contaminate you've got a brf cake you can slurry to start a second round right around the time your first bulk is reaching full colonization. Fruiting in layers (I.E. a new round every week) is the best way to ensure a contamination won't set you back too much. I use LCs all the time and have still never figured out why they get such a bad rep. :confused: The need the same autoclaved and still air conditions as anything else and if that's present you won't contam. It's faster then starting from spores and easier to get way more bulk. Agar is fantastic too! I mean really fantastic, you'll have so much fun and it has all the same advantages of LC plus you get to see contams wayyyy earlier. Have fun with it, go balls to the wall with it, have a blast and post some pin porn for us soon!
:dawerp:


--------------------
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: metaphoric]
    #21765271 - 06/05/15 10:06 AM (8 years, 7 months ago)

Thank you for the optimistic answer.

Now on to world domination..


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21771734 - 06/06/15 08:03 PM (8 years, 7 months ago)

So while I was inoculating the WBS jars some polyfill fell through but I still used my culture without noticing it until I was shaking it.. Also the polyfill was damp. My guess is from the pressure cooking

Any suggestions?


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OfflineBkillsU
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21771842 - 06/06/15 08:27 PM (8 years, 7 months ago)

You should be fine the poly won't hurt anythin.

Also, pics please :smile:


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: BkillsU]
    #21771972 - 06/06/15 08:58 PM (8 years, 7 months ago)

Pics coming soon

I covered the hole with micropore tape


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21772224 - 06/06/15 10:15 PM (8 years, 7 months ago)

Behold!


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21777896 - 06/08/15 09:17 AM (8 years, 7 months ago)

Two on the left are not usable? They look strange...

One on the right is my idea of a good looking LC perhaps not contam free. But better looking






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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21777909 - 06/08/15 09:20 AM (8 years, 7 months ago)

They seem pretty tough. And whatever is there is a lot of it. But the coloration is odd

I used light corn syrup


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21778043 - 06/08/15 10:03 AM (8 years, 7 months ago)

All 21 WBS jars and a few cakes in there also.. 4 of the grains are designated to be g2g jars



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OfflineSyntheticwords
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Re: The Road to 7 mono tubs *update* [Re: Syntheticwords]
    #21793993 - 06/11/15 05:19 PM (8 years, 7 months ago)

Behold! A few days after inoculation






All 21 jars are showing signs of growth similar to these with my g2g jars I plan to multiply the grow.
I plan to shake the hell out of the jars around 30-40% colonization

Now that I'm closer to the tub I've been thinking about my substrate
I want to use coir, hpoo, coffee grinds, gypsum but I am an idiot when it comes to ratios

Anyone want to chime in with their recipe?
Also I plan to use whole grain horse oats instead of WBS
I read a good article on them at http://www.shroomery.org/forums/showflat.php/Number/21121313#21121313


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Offlinetwistedty
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21794006 - 06/11/15 05:21 PM (8 years, 7 months ago)

good luck......

i want updates on this even if it fails.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: twistedty]
    #21794141 - 06/11/15 05:51 PM (8 years, 7 months ago)

It shall be done


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21797283 - 06/12/15 12:31 PM (8 years, 7 months ago)

Greetings humans

Colonization is increasing throughout all jars
Waiting to shake these babes around 30-40%

I have ordered agar, gypsum and oats for
Part 2 of the op


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21803850 - 06/13/15 09:08 PM (8 years, 7 months ago)

Here is how the colonization is going so far







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Offlinebugman
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21803857 - 06/13/15 09:10 PM (8 years, 7 months ago)

holy shit same picture


--------------------


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Offlinebugman
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Re: The Road to 7 mono tubs [Re: bugman]
    #21803863 - 06/13/15 09:11 PM (8 years, 7 months ago)

thought about doing outs but stuck to wbs let me know how oats are


--------------------


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OfflineDirdyD
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Re: The Road to 7 mono tubs [Re: bugman]
    #21804035 - 06/13/15 10:08 PM (8 years, 7 months ago)

I ran 7 tubs my first grow and things went pretty good (in my signature).

Ramping things up a bit this go 'round.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: bugman]
    #21804039 - 06/13/15 10:11 PM (8 years, 7 months ago)

I shall sire


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21823185 - 06/18/15 11:16 AM (8 years, 7 months ago)

GREETINGS HUMANS

Yesterday I shook the jars like a baby
Here are some updated pics

My G2G jars are almost 100% colonized how long should I wait before using?




Here are some of the ones I shaked. I left a few to colonize more before I shake them up





This go around I'm thinking of using whole grain horse oats or maybe ordering rye berrys or rye grass seed

Also I' plan on adding synthetic disc filters to these jars for a better experience then tyvek and coffee filters

Chime in on thoughts for bulk substrate ideas. I wasn't sure for my first grow if I should go with a 50:50 hpoo and straw or stick to just coir 

Any input is appreciated


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21823820 - 06/18/15 02:11 PM (8 years, 7 months ago)

Bump


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21823881 - 06/18/15 02:26 PM (8 years, 7 months ago)

If i were you id test the lc on agar. Nd i just made 24 jars, all but one is doing great!


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: kushroom]
    #21824315 - 06/18/15 04:11 PM (8 years, 7 months ago)

I'm past that  point haha all my LC is gone


Pay attention class!


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21826935 - 06/19/15 07:03 AM (8 years, 7 months ago)

Here is just 2 days after shaking like a baby



still debating on that sub choice... Feel free to chime in


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OfflineDirdyD
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21827205 - 06/19/15 08:40 AM (8 years, 7 months ago)

You'll likely get quicker and more informed suggestions from AMU.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: DirdyD]
    #21827646 - 06/19/15 10:54 AM (8 years, 7 months ago)

I shall not bother the GODS


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21828464 - 06/19/15 02:50 PM (8 years, 7 months ago)

Don't mind me, just following progress :grin:


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21830116 - 06/19/15 09:17 PM (8 years, 7 months ago)

Quote:

Syntheticwords said:
I shall not bother the GODS




DO bother the Gods, for without us, they have no power.  They're also historically jealous of our humanity and fleeting lives.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: DirdyD]
    #21838332 - 06/21/15 06:29 PM (8 years, 7 months ago)

Greetings humans

Today is a grand day

My g2g jars are fully colonized and I am preparing more batches of  WBS tonight

http://www.shroomery.org/forums/showflat.php/Number/16071143/fpart/1/vc/1

Using TL's Glc tek can i just use the syringe of mycelium water to speed up the colonizing of these grains as opposed to a g2g transfer?

Thank you


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21838647 - 06/21/15 07:33 PM (8 years, 7 months ago)

Some update pics COming soon


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21848209 - 06/23/15 10:39 PM (8 years, 7 months ago)

So I just got in a bag of 50/50 hpoo and straw mix from a vendor and I plan to crumble the cakes into a tray and stick in the sgfc

Meanwhile I await jars to cool for my first g2g transfer


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21848509 - 06/23/15 11:57 PM (8 years, 7 months ago)

Quote:

Syntheticwords said:
Greetings humans

Today is a grand day

My g2g jars are fully colonized and I am preparing more batches of  WBS tonight

http://www.shroomery.org/forums/showflat.php/Number/16071143/fpart/1/vc/1

Using TL's Glc tek can i just use the syringe of mycelium water to speed up the colonizing of these grains as opposed to a g2g transfer?

Thank you




just g2g all those into masters.

then make those masters into 5-10 more jars.  and spawn at least 5 qts spawn to each tub


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: twistedty]
    #21850431 - 06/24/15 01:12 PM (8 years, 7 months ago)

I just spawned a quart worth of brf cakes to 50/50 hpoo and straw into a tray

My main jars are nearing 100% colonization probably another 3 days


Here is the tray



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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21850445 - 06/24/15 01:15 PM (8 years, 7 months ago)

Tray is covered with aluminum foil with holes and a dome lid

I plan to Pop the lid everyday for 7-10 days while I wait for colonization

Then when I stick it in the sgfc and introduct it to pinning conditions I plan to put on a late casing after initial pins arise

Any thoughts on this brf tray. Go ahead and chime in


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21850630 - 06/24/15 02:05 PM (8 years, 7 months ago)

Cut your liner flush with the substrate. IT needs to shrink with the sub.


--------------------
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i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21851689 - 06/24/15 05:50 PM (8 years, 7 months ago)

It has been cut

Now we wait padawans

2 g2g jars created me a whopping 14 more jars

Hurray for g2g


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Invisible36fuckin5
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21852933 - 06/24/15 10:36 PM (8 years, 7 months ago)

I've gotten 40 jars from 2 masters before. Normal would be 10. But yeah, good job either way.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineBeyondScience
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21853234 - 06/24/15 11:50 PM (8 years, 7 months ago)

Good luck, I'm growing Shaggy Mane's and can barely get any colonization. I've tossed 1 round of BRF, 2 rounds of Rye. I think my moisture content is off, but I'll get there.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: BeyondScience]
    #21870012 - 06/28/15 07:02 PM (8 years, 6 months ago)

Greetings humans!

Great news. From my first batch of jars, 7 of the 14 are fully colonized  the others are shortly behind
How long should I wait before I use these fully colonized  grains? 3 days to consolidate or more?





Also here's what the g2g jars look like. I just stuck another jar into the fridge for the 3rd batch of g2g jars which will bring me up to my quota for 7 mono tubs! I must say that I've invested around $300 and plenty of time for these babies! I feel proud.





Meanwhile the brf cake/ hpoo tray is still locked up. Who the hell knows what could be going on in there. I vowed to not check until 10 days which will be the 4th. We will see then


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21873454 - 06/29/15 12:28 PM (8 years, 6 months ago)

Bump.


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21873845 - 06/29/15 02:23 PM (8 years, 6 months ago)

HI
No need to wait for grain jars to consolidate if they are 100% colonized you can spawn them to bulk aka your coir/verm that you are going to be using  for your monotub set up

You can have a quick peek at your tray but its best to leave it closed till around the 10 day mark


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Djchrist]
    #21874060 - 06/29/15 03:24 PM (8 years, 6 months ago)

Will post updates when I spawn the tub and check the tray


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OfflineTravelAgency
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Re: The Road to 7 mono tubs [Re: Djchrist]
    #21874102 - 06/29/15 03:34 PM (8 years, 6 months ago)

Quote:

Djchrist said:
HI
No need to wait for grain jars to consolidate if they are 100% colonized you can spawn them to bulk aka your coir/verm that you are going to be using  for your monotub set up

You can have a quick peek at your tray but its best to leave it closed till around the 10 day mark





Do you still need to wait for consolidation for brf cake to bulk?


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OfflineDjchrist
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Re: The Road to 7 mono tubs [Re: TravelAgency]
    #21876129 - 06/29/15 11:40 PM (8 years, 6 months ago)

NO, the  only reason consolidation is needed is for the mycelium to take a good hold on the substrate its colonizing before fruiting.If the brf cake's were going to be the substrate yes if you spawning to bulk its not needed


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Re: The Road to 7 mono tubs [Re: Djchrist]
    #21877730 - 06/30/15 11:47 AM (8 years, 6 months ago)

My brf cakes had a mushroom or two on each of them before I crumbled to my poo tray


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21880927 - 06/30/15 11:42 PM (8 years, 6 months ago)

Won't be the end


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Re: The Road to 7 mono tubs [Re: Djchrist]
    #21883194 - 07/01/15 02:51 PM (8 years, 6 months ago)

Update on TRAY!!!! :smile:

Have you spawned your jars to bulk yet ??


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Djchrist]
    #21883592 - 07/01/15 04:11 PM (8 years, 6 months ago)

Greetings humans!

I am currently in he process of pasteurizing some coir/Verm and a little bit of gypsum



franks tek ofcourse. I owe most of this op to him and countless others

The brf/ hpoo tray will be checked on July 4th! 10 days from when I spawned it.
I'm not sure what to expect. We will see then


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21883618 - 07/01/15 04:17 PM (8 years, 6 months ago)

Also here is the chosen spawn

I have 10 fully colonized jars

The question is if I should spawn 7qts per tub or 5 qts per tub
I have about 10 qts of coir

Also I shook up the 2nd batch of WBS g2g jars. They were all around 40-50% done


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Re: The Road to 7 mono tubs [Re: twistedty]
    #21883776 - 07/01/15 04:56 PM (8 years, 6 months ago)

When you say "a few days", how many days exactly?


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21883779 - 07/01/15 04:56 PM (8 years, 6 months ago)

Here's the monotub I just made for it

8 holes total all 1 inch



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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21883793 - 07/01/15 04:59 PM (8 years, 6 months ago)

A few days? What you mean


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21884245 - 07/01/15 06:37 PM (8 years, 6 months ago)

Bump. Any input fellows


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21884776 - 07/01/15 08:04 PM (8 years, 6 months ago)

Quote:

Syntheticwords said:
Bump. Any input fellows




What kind of input are you looking for? Looks like you've got it under control so far! :thumbup:

If I could add one quick note; when your inoculating with LC you should shake immediately after you inoculate. When you read about people suggesting not to shake right away they're talking about MS injections, where the germination process still has too take place. You've already covered that in a completed LC.


--------------------
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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: metaphoric]
    #21884881 - 07/01/15 08:26 PM (8 years, 6 months ago)

Thanks for the info!!

Waiting for my coir to cool down


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21885324 - 07/01/15 10:05 PM (8 years, 6 months ago)

Quick question

My coir moisture content seems slightly on the wet side

Is this a bad thing?


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21887784 - 07/02/15 12:45 PM (8 years, 6 months ago)

Greetings humans!! Here's an update on the poo tray!











Is this ready to fruit or what?


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21887792 - 07/02/15 12:48 PM (8 years, 6 months ago)

Also forgot to mention that two monotubs have been spawned today



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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21887854 - 07/02/15 01:04 PM (8 years, 6 months ago)

If it didn't ran a stream when you squeezed it in you your hands you should be good

That tray is so ready for fruiting  :smile:


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Djchrist]
    #21887915 - 07/02/15 01:23 PM (8 years, 6 months ago)

Looking for input on those trays still

Bump


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21888217 - 07/02/15 02:35 PM (8 years, 6 months ago)

So far here are my ideas

I put a dome lid that fits with aluminum tray, because it came with it and I planned to poke a hole through the middle and stuffing polyfill or maybe using a synthetic filter disc any thoughts on that?

Other option includes building a fruiting chamber for this bad boy.

Should I late case? Or not case them at all


Any input is appreciated


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21888229 - 07/02/15 02:38 PM (8 years, 6 months ago)

Quote:

Syntheticwords said:
Looking for input on those trays still

Bump




Pull the uncolonized bits of straw out. Then giver aanother day.


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Pastywhyte]
    #21888247 - 07/02/15 02:43 PM (8 years, 6 months ago)

Thanks pasty!

I will be using your agar tek to clone some of these fruits

Should I take a spore print or cut tissue? Either will work I'm sure


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21894470 - 07/03/15 08:46 PM (8 years, 6 months ago)

looks good to me and my coir is always slightly wet but it works fine the mycelium sucks it don pretty good


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: bugman]
    #21894572 - 07/03/15 09:08 PM (8 years, 6 months ago)

Behold humans! I am preparing for my last batch of g2g

I am moving to Colorado in the beginning of August and I just spawned my first two tubs

I'm nervous about the results as the others follow soon. Totaling up to my goal of 7 tubs

Hopefully I can get these all flushed out by then
If not I am not alone in my ventures. I'm sure my work will be carried on


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21897018 - 07/04/15 02:47 PM (8 years, 6 months ago)

Here's an update humans


This  is two days in







Both tubs seem to be speeding. Not sure if this is fast or not.
I used a 1:2 ratio


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21897026 - 07/04/15 02:49 PM (8 years, 6 months ago)

Also that tray will be put into fruiting tomoro.

I'm excited for that :smile:


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Offlinebugman
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21897204 - 07/04/15 03:34 PM (8 years, 6 months ago)

Quote:

Syntheticwords said:
Here's an update humans


This  is two days in







Both tubs seem to be speeding. Not sure if this is fast or not.
I used a 1:2 ratio



looks about as fast as mine they usualy ready to fruit at day 4 or 5 and in 3 days pinning have you tried the oats yet


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21897463 - 07/04/15 04:41 PM (8 years, 6 months ago)

Nope! Just stuck with the WBS


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21900991 - 07/05/15 01:10 PM (8 years, 6 months ago)

yea so did i i know what im doing with it


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: bugman]
    #21901040 - 07/05/15 01:19 PM (8 years, 6 months ago)

Yeah my results haven't proved me wrong yet!


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21905844 - 07/06/15 03:58 PM (8 years, 6 months ago)

Greetings humans

Another update:

My 2nd batch of jars are all almost 100% colonized



While my 3rd batch of jars have just begun colonizing






Everything is moving pretty smoothe. It's a shame I am moving soon and I have to stop in the middle of my groove.
My tubs are colonizing still
And my tray is getting introduced to its terrarium tomorrow

Well on the way to 7 tubs


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21906044 - 07/06/15 04:46 PM (8 years, 6 months ago)

:popcorn:


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: shellzenone]
    #21909849 - 07/07/15 01:54 PM (8 years, 6 months ago)

Greetings humans.

Today I introduced my brf/hpoo tray into its new home






My tubs are still rolling
How do they look after 5 days?





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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21910229 - 07/07/15 03:33 PM (8 years, 6 months ago)

I'm assuming after the top has been white for a few days it will be safe to fruit


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OfflineSyntheticwords
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21915189 - 07/08/15 02:01 PM (8 years, 6 months ago)

Tubs are still moving along fine. Looking for input on fruiting signs


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21916376 - 07/08/15 06:56 PM (8 years, 6 months ago)

everything looks in order.  should have some pins later this week


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Re: The Road to 7 mono tubs [Re: twistedty]
    #21917129 - 07/08/15 09:29 PM (8 years, 6 months ago)

Thanks twisted! Here are some pics as I was stuffing polyfill

Here are both of them



What you guys think? Too early or just right on time?


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21921425 - 07/09/15 07:15 PM (8 years, 6 months ago)

Behold all the white!!




Looks like it's about to start pinning?


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21921769 - 07/09/15 08:36 PM (8 years, 6 months ago)

cant tell get a shot inside the tub


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Re: The Road to 7 mono tubs [Re: bugman]
    #21922281 - 07/09/15 09:51 PM (8 years, 6 months ago)

I fear popping the hood


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21929547 - 07/11/15 04:16 PM (8 years, 6 months ago)

I stuffed with polyfill on July 8th

Are these guys needing air?



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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21929957 - 07/11/15 05:57 PM (8 years, 6 months ago)

just leave it for now do you have a fan running?


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Re: The Road to 7 mono tubs [Re: bugman]
    #21929995 - 07/11/15 06:10 PM (8 years, 6 months ago)

Awesome!


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Re: The Road to 7 mono tubs [Re: mr. top hat]
    #21930364 - 07/11/15 07:09 PM (8 years, 6 months ago)

Yup. A fan running in the room, not directly at it. But in the room


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21931060 - 07/11/15 09:58 PM (8 years, 6 months ago)

then you should be good


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Re: The Road to 7 mono tubs [Re: bugman]
    #21931290 - 07/11/15 11:01 PM (8 years, 6 months ago)

The patience begins I suppose....


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21931448 - 07/11/15 11:40 PM (8 years, 6 months ago)

I don't see any condensation on the sides.  What is the RH?


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Re: The Road to 7 mono tubs [Re: ShroominMe]
    #21931599 - 07/12/15 12:13 AM (8 years, 6 months ago)

No way of telling. Here it is on outside



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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21931613 - 07/12/15 12:19 AM (8 years, 6 months ago)

Aah that looks much better! :thumbup: Rock on, humanoid! :rockon:


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Re: The Road to 7 mono tubs [Re: ShroominMe]
    #21933662 - 07/12/15 02:04 PM (8 years, 6 months ago)

I loosened up the top holes

How long do you guys wait before pinning occurs??im only on day 4 of fruiting conditions


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21934065 - 07/12/15 03:51 PM (8 years, 6 months ago)

also two more tubs should be spawned by tomoro

Pics later today of the poo+ brf tray


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Re: The Road to 7 mono tubs *quick question * [Re: Syntheticwords]
    #21935465 - 07/12/15 10:42 PM (8 years, 6 months ago)

Hey humans!

I have water in my tub... It's not in direct contact with the substrate

What to do?


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Re: The Road to 7 mono tubs *quick question * [Re: Syntheticwords]
    #21935472 - 07/12/15 10:44 PM (8 years, 6 months ago)

Go smoke a bowl :pipesmoke:


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Re: The Road to 7 mono tubs *quick question * [Re: Pastywhyte]
    #21935483 - 07/12/15 10:47 PM (8 years, 6 months ago)

I am currently smoking a bowl

Can't wait for that colorado weed :smile:


But also. I have water in my tubs. Suggestions? Or should I continue smoking and not worry until harvest...


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Re: The Road to 7 mono tubs *quick question * [Re: Syntheticwords]
    #21935494 - 07/12/15 10:49 PM (8 years, 6 months ago)

Quote:

Syntheticwords said:
continue smoking and not worry until harvest...




:super:


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Re: The Road to 7 mono tubs *quick question * [Re: Pastywhyte]
    #21935503 - 07/12/15 10:52 PM (8 years, 6 months ago)

Speaking of harvest... How long is TOO long before pinning... I am nervous at this point not knowing what to expect. Or if to expect anything

I suppose nature will pump out when she is ready :shroomin:


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Re: The Road to 7 mono tubs *quick question * [Re: Syntheticwords]
    #21935505 - 07/12/15 10:54 PM (8 years, 6 months ago)

I have seen classic cubes take 3 weeks. I have seen PE take over a month. Those of course are not common but still possible.


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Re: The Road to 7 mono tubs *quick question * [Re: Pastywhyte]
    #21935519 - 07/12/15 10:59 PM (8 years, 6 months ago)

Excellent. That means I shouldn't be expecting just yet ..

Thanks pasty!


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Re: The Road to 7 mono tubs *quick question * [Re: Syntheticwords]
    #21935877 - 07/13/15 01:41 AM (8 years, 6 months ago)

Quote:

Syntheticwords said:
Excellent. That means I shouldn't be expecting just yet ..



Congratulations!


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Re: The Road to 7 mono tubs *quick question * [Re: ShroominMe]
    #21936420 - 07/13/15 08:45 AM (8 years, 6 months ago)

Lmao!!! That's hilarious



Two more tubs being spawned today!


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Re: The Road to 7 mono tubs *quick question * [Re: Syntheticwords]
    #21936702 - 07/13/15 10:26 AM (8 years, 6 months ago)

My bulk substrate was too hot to spawn.

I read that between 84-90 is range I should spawn to bulk.

For times sake and the fact that my spawn is soaking I put those puppies in the freezer


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Re: The Road to 7 mono tubs *quick question * [Re: Syntheticwords]
    #21936999 - 07/13/15 11:55 AM (8 years, 6 months ago)

your getting closer patience is key


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Re: The Road to 7 mono tubs *quick question * [Re: bugman]
    #21937100 - 07/13/15 12:19 PM (8 years, 6 months ago)

I will be doing what you are doing except smaller scale in a couple weeks. Nice looking grow, ill be following it. :thumbup:


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Re: The Road to 7 mono tubs *quick question * [Re: kibblesnibble] * 1
    #21937289 - 07/13/15 01:13 PM (8 years, 6 months ago)

Greetings humans


Today I have spawned two more tubs...




Here is an update pic of tub 1 as of this morning





Here is a update pic of tub 2. I put this one into fruiting today. 5 days of extra "consolidation" for my experiment to check and see if there is a difference between the two
My hypothesis is that it doesn't matter. Nature will push out when she's ready



I cased my tray this morning also, with pasteurized coir and Verm  An attempt to initiate pinning. And partial experimentation on the affection of casing.



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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21951786 - 07/16/15 12:19 PM (8 years, 6 months ago)

Greetings humans.

Today is a great day!

Upon checking my tub I spawned on July 2nd and introduced fruiting on the 8th "tub 1"
I  have begun to notice knots!! I will post pictures when they become grown pins


tub 2 is too high for me to check... Neighbor has my ladder, so I will check it when I can


However I am about to go on a small vacation!!
Going to camp on the beach on Florida for the next few days...perfect timing for these babies to be coming in

It's funny how nature works. Waiting for the right moment...


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21952417 - 07/16/15 03:13 PM (8 years, 6 months ago)

In just 3 hours from a knot, here is a pin



Il update with hopefully some pin porn when I come back from fthe beach


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21952421 - 07/16/15 03:14 PM (8 years, 6 months ago)

I just realized you can't see shit in the picture...

However my tub is filled with knots. So I will update!!


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21958345 - 07/17/15 09:59 PM (8 years, 6 months ago)

florida!!!!!!!!!!!


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Re: The Road to 7 mono tubs [Re: bugman]
    #21958749 - 07/17/15 11:43 PM (8 years, 6 months ago)

So where should I begin...
Florida was a complete disaster
Drove 4 hours there and back basically
Don't even want to start haha


But good news...



I will say that my bin has lost a considerable amount of condensation since Ive been gone
Is this because of the pinning/fruiting stage?


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21958772 - 07/17/15 11:50 PM (8 years, 6 months ago)

I will update with more pin porn in the morning :smile:

I'm not exactly sure how my pin set is

This is a MS inoculation so I know I'm basically up in the air with that

However I have many knots and pins throughout my tub

so I will update with my first flush of tub 1


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21959757 - 07/18/15 09:21 AM (8 years, 6 months ago)

Any input on the condensation during pinning?


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21959839 - 07/18/15 09:52 AM (8 years, 6 months ago)

I also taped up some holes for my tray...
I believe it's not humid enough

ALSO I HAVE VERY BAD NEWS
Tub 2 has come down with trich

Living in the city, can not exactly bury it around here

I was thinking of ripping the trich out with a plastic bag and shoving my tub somewhere far far away


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21959959 - 07/18/15 10:44 AM (8 years, 6 months ago)

Any takers :heytherebadboy:


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21960045 - 07/18/15 11:05 AM (8 years, 6 months ago)

nice quintuple bump :crazy:

dont tape holes its counterproductive. condensation is a temp difference thing.


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Re: The Road to 7 mono tubs [Re: spacechildo]
    #21960403 - 07/18/15 12:35 PM (8 years, 6 months ago)

I apologize for the bumps

I removed the tub and will be burying it
Should I cut the trich out?


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21960591 - 07/18/15 01:17 PM (8 years, 6 months ago)

what trich? if you have it dont cut it, just throw out the sub.
we prevent contams we dont fight them.


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Re: The Road to 7 mono tubs [Re: spacechildo]
    #21961036 - 07/18/15 03:28 PM (8 years, 6 months ago)

I buried it....



It was annoying as hell digging a hole. Many things in the way. I can't imagine I enjoyed it as a kid



Here is the tub 1 pinning up



A lot seem to be on the sides


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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21965480 - 07/19/15 02:41 PM (8 years, 6 months ago)

Here is a current pic



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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21966136 - 07/19/15 04:36 PM (8 years, 6 months ago)

3 hours later....



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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21966158 - 07/19/15 04:43 PM (8 years, 6 months ago)

More pin porn!




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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21969025 - 07/20/15 03:03 AM (8 years, 6 months ago)

Looking good! :thumbup:

Have you thought about how you're going to celebrate harvesting your first fruits? :mushroom2:


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Re: The Road to 7 mono tubs update* [Re: ShroominMe]
    #21969883 - 07/20/15 09:22 AM (8 years, 6 months ago)

Here is an update for today humans!

I put this brf/ hpoo tray into fruiting on June 24th
After no action, I plugged up some holes and applied a casing layer on July 13th

Today, 7 days later, here are the first pins of that tray





Also more pin porn from tub 1





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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21969887 - 07/20/15 09:23 AM (8 years, 6 months ago)

I plan on testing the potency by going on a kayaking trip :smile::shroomin:


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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21972636 - 07/20/15 08:08 PM (8 years, 6 months ago)

Which one would you clone?



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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21972650 - 07/20/15 08:10 PM (8 years, 6 months ago)

The first cluster that came up. Any clusters of 3 or more. Meaty fruits clustered together. Clone clusters :awesomenod:


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Re: The Road to 7 mono tubs update* [Re: Pastywhyte]
    #21972699 - 07/20/15 08:19 PM (8 years, 6 months ago)

To clone these babies I cut a piece of tissue from the stem and leave In ziplock bag to fuzz up before transferring to agar?


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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21972709 - 07/20/15 08:23 PM (8 years, 6 months ago)

For small pins you can put them right on the agar and transfer the leading edge away from the contams. It will usually be very aggressive and overgrow the bacteria.

Larger pins can be torn in half and a small amount of tissue scraped from the center and put right into the agar. No need for ziploc bags IMO.


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Re: The Road to 7 mono tubs update* [Re: Pastywhyte]
    #21972776 - 07/20/15 08:40 PM (8 years, 6 months ago)

Looks like I will need to prepare some petri plates. I'll be using your recipe just pouring into plastic plates rather then the mini rounds


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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21972890 - 07/20/15 09:01 PM (8 years, 6 months ago)

LOL yeah PDA is pretty standard. Remember to change the recipe if you want to keep the culture vigorous long term. I also like grain water, malt extract, corn starch and honey, brf agar with karo, dog food. Mix it up :thumbup:


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Re: The Road to 7 mono tubs update* [Re: Pastywhyte]
    #21972925 - 07/20/15 09:07 PM (8 years, 6 months ago)

Change the recipe as in add everything that you have mentioned?

I'll be moving to Colorado soon, and I would love to keep a genetic disc of information with me, if you know what I mean

That way I can start off where
I left off...essentially


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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21972997 - 07/20/15 09:17 PM (8 years, 6 months ago)

Just mix it up. For these plates make the PDA (potato and sugar source), next round do grain water, next try malt extract. A rotation will keep the culture vigorous.


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Re: The Road to 7 mono tubs update* [Re: Pastywhyte]
    #21974840 - 07/21/15 09:20 AM (8 years, 6 months ago)

Here are current pictures my humans

It seems that my second flush is moving in during the end of my first flush...
I suppose I'll let these grow out aswell before picking them




So I know I have to twist and pull to get these suckers out...the question is after pulling do you guys "patch up" the torn substrate by putting Verm or coir or something of that

Thank you


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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21975097 - 07/21/15 10:36 AM (8 years, 6 months ago)

looking good man! pump up the FAE now until harvest. they'll love it!

Dont rip up your substrate, it will be a great spot for contams to germinate on.
I cut mine with a scissors, as close to the base as you can get so you dont leave much mushroom flesh behind.

and no, the stumps wont rot :wink:


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Re: The Road to 7 mono tubs update* [Re: spacechildo]
    #21975259 - 07/21/15 11:23 AM (8 years, 6 months ago)

Well I went ahead and removed the polyfill from the top holes

Hopefully they will reward me nicely haha

I've read about cutting at the base aswell

I'm not sure if I can twist and pull without ripping up the substrate

That's my main goal. Keeping the tub flushing out with no contams


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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21975301 - 07/21/15 11:38 AM (8 years, 6 months ago)

some mushrooms you can, some you cant. at least not without ripping up some substrate.


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Re: The Road to 7 mono tubs update* [Re: spacechildo]
    #21975326 - 07/21/15 11:45 AM (8 years, 6 months ago)

I have been doing just twist and pull with zero issues. The divots seem to be a non issue. If you case they tend to come out a little cleaner but its not been an issue for me really. I actually have less contam issues than I did with the stumps.


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Re: The Road to 7 mono tubs update* [Re: Pastywhyte]
    #21975359 - 07/21/15 11:57 AM (8 years, 6 months ago)

maybe you're more careful than I am when doing it, or maybe I am too reckless :shrug:

Just to confirm for myself and OP you're not saying leaving stumps cause you trouble? being how they're just myc and all..


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Re: The Road to 7 mono tubs update* [Re: spacechildo]
    #21975369 - 07/21/15 12:00 PM (8 years, 6 months ago)

Mold seems to like latching onto stumps for me. I have yet to see it start in a divot.


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Offlinespacechildo
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Re: The Road to 7 mono tubs update* [Re: Pastywhyte]
    #21975384 - 07/21/15 12:08 PM (8 years, 6 months ago)

do you ever see exposed grains in your divots?
I pump up fae and dont water the sub the last couple days before harvest
so the sub/myc is a little dry and tears off in pieces, kinda like wallpaper for me, I tear off more than I'm trying to.

My stumps are where most new pins/knots form, but they usually dont do much until 3rd flush or so.


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Re: The Road to 7 mono tubs update* [Re: spacechildo]
    #21975430 - 07/21/15 12:24 PM (8 years, 6 months ago)

I may case the tub if it offers the most benefits, vs no casing


Also before I rehydrate the substrate, I should wait for the pins coming in right now to grow fully?


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21975441 - 07/21/15 12:26 PM (8 years, 6 months ago)

Almost as if flush 1 and 2 are coming in at same time


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21975478 - 07/21/15 12:38 PM (8 years, 6 months ago)

Quote:

Syntheticwords said:
Almost as if flush 1 and 2 are coming in at same time




That is normal.

Also space I find that when  I case I never see exposed grains but even when I don't its not a problem.  The myc usually takes over in a day or two before molds have a chance to germinate.


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Re: The Road to 7 mono tubs update* [Re: spacechildo]
    #21975977 - 07/21/15 03:02 PM (8 years, 6 months ago)

Quote:

spacechildo said:
looking good man! pump up the FAE now until harvest. they'll love it!

Dont rip up your substrate, it will be a great spot for contams to germinate on.
I cut mine with a scissors, as close to the base as you can get so you dont leave much mushroom flesh behind.

and no, the stumps wont rot :wink:




Well shit thats a good tip, i been ripping mine of nd the subs are hating me for it. I hit trech on one tub yesterday due to that same reason im thinking. Oh well?:shrug: It wont bruise the mushrooms to bad cutting them will it?


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Re: The Road to 7 mono tubs update* [Re: kushroom]
    #21977157 - 07/21/15 07:37 PM (8 years, 6 months ago)

I plan on harvesting these babies within the hour or so and I'll be testing the potency if you know what I mean :wink:

I'll be as gentle as possible and cut if necessary. Taking a spore print and I plan on making some agar plates tonight :smile: gonna clone the meatiest of the clusters



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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21977287 - 07/21/15 08:01 PM (8 years, 6 months ago)




Soon...


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Re: The Road to 7 mono tubs update* [Re: Syntheticwords]
    #21977321 - 07/21/15 08:08 PM (8 years, 6 months ago)

Quote:

Syntheticwords said:



Soon...



They look great! Let us know how your science experiment goes :smile:


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Re: The Road to 7 mono tubs [Re: Psilopsychic]
    #21978075 - 07/21/15 10:55 PM (8 years, 6 months ago)

I feel like you would have more success using agar to colonize maybe 10 or so WBS jars, then do G2G with 4 of those jars.  Those 4 could make you around 40 or so jars of fully colonized WBS, plus 6 left over jars for future use. I don't really see to point in making so many LC's.


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Re: The Road to 7 mono tubs [Re: uninc4life2010]
    #21978175 - 07/21/15 11:22 PM (8 years, 6 months ago)

I stopped using lcs after the first batch was knocked up


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21979536 - 07/22/15 09:14 AM (8 years, 6 months ago)

Alright you humans


I gathered my tissue clone sample, they are soaking in blue

Is this acceptable to transfer to agar?

Also picked 300grams  wet last night:heytherebadboy:


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21979673 - 07/22/15 09:56 AM (8 years, 6 months ago)

I don't soak tissue transfers in anything. Take a clean inner sample and put it right on the plate.


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Re: The Road to 7 mono tubs [Re: Pastywhyte]
    #21979741 - 07/22/15 10:15 AM (8 years, 6 months ago)

Well this sample came straight from the mushroom

By soaking in blue I mean the actives are showing


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21979755 - 07/22/15 10:18 AM (8 years, 6 months ago)

Here they are along from where the sample came from




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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21979766 - 07/22/15 10:22 AM (8 years, 6 months ago)

Those tissue samples are in a ziplock bag. I put them in yesterday, while making my plates / currently waiting for them to cool off


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21983630 - 07/23/15 08:42 AM (8 years, 6 months ago)

Greetings humans

My petri plates melted in pc. Should have known better. I'll be using some glad mini rounds instead

Total harvest weight from tub one's first flush= 540 grams wet

The brf/ poo tray is really started to take off, applying the casing layer gave exactly what the mushies wanted

Coming up next folks, we have 3 more tubs put into fruiting yesterday morning, expecting pins in 7 days


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21989026 - 07/24/15 09:13 AM (8 years, 6 months ago)

Greetings humans! The brf/ poo tray has come to life! check that cluster out! best believe it's getting cloned




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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21989109 - 07/24/15 09:38 AM (8 years, 6 months ago)

That cluster is a whopping 80grams wet!


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21989923 - 07/24/15 11:50 AM (8 years, 6 months ago)

Nice mushies ya got there. Keep us updated on the agar plates. You gonna use Pasty's tek?

And, I didn't know you could store tissue samples in the fridge. I thought it had to transferred to the plate immediately. Anyways, happy growin'!! :elmo:


Edited by Psilosoulful (07/24/15 11:53 AM)


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Re: The Road to 7 mono tubs [Re: Psilosoulful]
    #21990422 - 07/24/15 02:08 PM (8 years, 6 months ago)

Oh yes I will be using his tek

And I have limited time so I placed the tissue samples in plastic bags and placed in fridge to transfer on sunday


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21994101 - 07/25/15 08:40 AM (8 years, 6 months ago)

Another Christmas morning you guys

Here is another flush from tub 1, also the tray popped out again

Still patiently waiting for the next tubs to start pinning up



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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21994115 - 07/25/15 08:45 AM (8 years, 6 months ago)

:Awemazing:


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21995248 - 07/25/15 01:19 PM (8 years, 6 months ago)

So the 2nd harvest of tub 1 is 440 grams wet

The tray pushed out 70grams wet with this initial pinset


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21999386 - 07/26/15 10:59 AM (8 years, 6 months ago)

Alright guys! Tub 3 and 4 have started pinning :smile:

Tub 1 is still preparing for a 3rd flush pushed out 1130grams wet so far
Tub 2= FUCKING DIED
Tub 3= pinning
Tub 4= pinning

This morning I transferred 7 tissue samples to 7 pasty plates :smile:

I will be shutting this thread down and starting a new series

I am at the point where it's just going to rinse and repeat
So I'll be starting a new thread in the future here :smile:
Thanks for sticking by and lurking!


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21999400 - 07/26/15 11:02 AM (8 years, 6 months ago)

Rest in peace tub 2


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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21999605 - 07/26/15 11:40 AM (8 years, 6 months ago)

Quote:

Syntheticwords said:
This morning I transferred 7 tissue samples to 7 pasty plates :smile:



:jackiechanofapproval:


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Re: The Road to 7 mono tubs [Re: Psilosoulful]
    #22014418 - 07/29/15 11:07 AM (8 years, 6 months ago)

Haha I forgot about these old g2g jars j

They did not forget me



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