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Doctor Mycelium
I've Heard It Both Ways



Registered: 06/19/13
Posts: 58
Loc: South Georgia
Last seen: 6 years, 7 months
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Would it be an error?
#21736922 - 05/29/15 01:48 PM (8 years, 7 months ago) |
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Hello. I am considering trying to grow mushrooms from the field that I frequent in a grain substrate. I have encountered the farmer in the field far too many times now and I am tired of taking the risk. Basically, is this a viable option? Is it a bad idea?
The mushrooms that I pick are P. Cubensis. My plan is to pick some and make spore prints onto a piece of tin foil. Then, I would scrape the print off of the foil with a clean razor and mix with water in a needle. I'd inject the needle into a substrate mix (either pre-made or I will make my own, depending on what is necessary to make this work). If I am careful with sterilization, then would there be a problem with this method?
(I have been trying to research into this myself, but am not having much luck. Can anyone here attest to trying it? Perhaps, even share your results? Thanks a lot.)
I can supply any additional information. Thanks for your time and input!
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DiggerOD
Stranger


Registered: 10/22/12
Posts: 8
Loc: N.Ireland.
Last seen: 3 years, 9 months
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Hi, it's a great idea! However making spore syringes from caps taken from a field would have a fairly high risk of contamination which would write-off your grow. I would recommend researching germinating spores on agar filled Petri dishes so you can isolate a pure sample of mycelium with which to inoculate your grain. Much good information on this site. Don't be too disheartened if you experience a bit of failure though, it happens to us all at some point, no matter how careful we are. Good luck.
Edited by DiggerOD (05/29/15 02:01 PM)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Would it be an error? [Re: DiggerOD]
#21736996 - 05/29/15 02:08 PM (8 years, 7 months ago) |
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I'd just get some agar and clone a fruit. You'll have better results.
And yes, do all of your own work. Not only are kits expensive but you can't know how they were prepped, so if something goes wrong you'll have no idea why.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Doctor Mycelium
I've Heard It Both Ways



Registered: 06/19/13
Posts: 58
Loc: South Georgia
Last seen: 6 years, 7 months
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Re: Would it be an error? [Re: DiggerOD]
#21736999 - 05/29/15 02:10 PM (8 years, 7 months ago) |
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Thank you very much for your timely response and encouragement. I will certainly look into the Petri dish germination. I will continue to use the site as a reference and a tool for help.
Is there something I could do to lessen the chances of contamination in the mean time while I isolate a pure sample? Perhaps, sterilizing the foil and maybe even the mushroom or spores themselves? I apologize if this is a noob question, but I would be interested in trying both methods. Thanks and be well!
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Doctor Mycelium
I've Heard It Both Ways



Registered: 06/19/13
Posts: 58
Loc: South Georgia
Last seen: 6 years, 7 months
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Thank you for your response, as well. I will certainly look into cloning. It sounds promising.
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DiggerOD
Stranger


Registered: 10/22/12
Posts: 8
Loc: N.Ireland.
Last seen: 3 years, 9 months
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Never be afraid to seek knowledge! I found that making a Still Air Box (SAB) really helped. Again I'd say look it up in this site, it would be a lot of typing to go through it all and I'm a lazy git. Also get yourself a little alcohol lamp to flame sterilise your foil and blade. Great call on the tissue clone idea too. Be sure to take tissue from inside the stem, not the surface.
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DiggerOD
Stranger


Registered: 10/22/12
Posts: 8
Loc: N.Ireland.
Last seen: 3 years, 9 months
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Forgot to say, whichever method you use, definitely get competent at taking spore prints. That way you can store them and start a future grow at a time that suits you. That's the main reason I suggested agar. Take a few every crop so you have a fresh stock to work from. Sometimes it just isn't practical to start a grow at the same time you are harvesting. Life can be busy.
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Doctor Mycelium
I've Heard It Both Ways



Registered: 06/19/13
Posts: 58
Loc: South Georgia
Last seen: 6 years, 7 months
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Re: Would it be an error? [Re: DiggerOD]
#21741310 - 05/30/15 02:36 PM (8 years, 7 months ago) |
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Thank you once again, Digger. I will certainly look more into and follow your advice. Be well!
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