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OfflineLawnboy1185
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Registered: 03/30/15
Posts: 119
Last seen: 7 years, 3 months
Contamination problems....HELP!!!!!!
    #21736596 - 05/29/15 12:11 PM (8 years, 7 months ago)

first grow......3rd attempt.

10 jars are almost 4 weeks in and today I noticed contamination.  It looks like rat shit...and there is condensation inside the jars?  I'm not sure how the jars got wet on the inside, but 7 out of the 10 jars have signs of mold.

Why is this so hard?!?!?!?   

PF tek....directions to a T, stored in an open closet at room temp, 72-81 degrees

HELP?!?!?!?


The only jar without contamination looks like it started fruiting before colonization was complete.


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OfflineSlimeySlugNug
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Registered: 05/28/15
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Loc: Negril Jamaica
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736615 - 05/29/15 12:18 PM (8 years, 7 months ago)

Dang that is interesting, definitely looks like some kind of contamination


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OfflineMonkeyJesusFresco
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Registered: 10/09/12
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736639 - 05/29/15 12:25 PM (8 years, 7 months ago)

Quote:

Lawnboy1185 said:

The only jar without contamination looks like it started fruiting before colonization was complete.




hey...

guess what pinning before full colonization is a sign of?


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OfflineLawnboy1185
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Registered: 03/30/15
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Re: Contamination problems....HELP!!!!!! [Re: MonkeyJesusFresco]
    #21736672 - 05/29/15 12:31 PM (8 years, 7 months ago)

I'm all ears everyone?!


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Invisiblemush madness
absorbing everything
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Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: Contamination problems....HELP!!!!!! [Re: MonkeyJesusFresco]
    #21736679 - 05/29/15 12:33 PM (8 years, 7 months ago)

I know I know pick me lol


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InvisiblePFFFT


Registered: 08/31/09
Posts: 621
Loc: Velvet Underground
Re: Contamination problems....HELP!!!!!! [Re: SlimeySlugNug]
    #21736685 - 05/29/15 12:34 PM (8 years, 7 months ago)

yup.. all your jars are f-up:awesomenod:
that "rat shit" is bacteria IMO. and cause of it might be too wet substrate you made..

i think you need to do better at sterilization methods.molds can be from contaminated print or if you made syringe or even if you done it clean-but inoculated in open air and mold spores came in thru holes in lid ect.there is tons of ways to gget contams ,but only one to prevent it - really good sterile tech!

but do not worry- you will learn how to do it. 3rd- not last. :wink:
just try again, toss contaminated jars and try improve sterility next time!:super:


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736686 - 05/29/15 12:35 PM (8 years, 7 months ago)

What was your sterile procedure from brf prep through inoculation. Maybe there is something you over looked.


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OfflineLawnboy1185
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Re: Contamination problems....HELP!!!!!! [Re: insanemike]
    #21736728 - 05/29/15 12:44 PM (8 years, 7 months ago)

PC for 90 minutes, let sit over night.  Made a glove box and used rubber gloves and a long sleeve shirt.  Sprayed the inside of the glove box and the area of my kitchen with 70% alcohol.

I made 1 quick transfer from the pc to the glove box.  And removed the foil before knock up inside the glove box.  After I was done innoculating I let them sit in the glove box for 4 or 5 minutes, then transferred into the original jar box and placed on a top shelf of an open closet.


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InvisiblePFFFT


Registered: 08/31/09
Posts: 621
Loc: Velvet Underground
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736754 - 05/29/15 12:51 PM (8 years, 7 months ago)

contaminated syringe ? :shrug:


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Contamination problems....HELP!!!!!! [Re: PFFFT]
    #21736778 - 05/29/15 12:56 PM (8 years, 7 months ago)

Quote:

PFFFT said:
contaminated syringe ? :shrug:




That's what I was thinking also.

Do not use a glovebox. Use this
http://www.shroomery.org/forums/showflat.php/Number/20048771

lower your contamination rate with this.
http://www.shroomery.org/forums/showflat.php/Number/19208976

and speed up your colonization times with this.
http://www.shroomery.org/forums/showflat.php/Number/20429745/page/1


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OfflineSyntheticwords
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Registered: 08/13/11
Posts: 375
Last seen: 6 years, 9 months
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736818 - 05/29/15 01:07 PM (8 years, 7 months ago)

Did you alcohol the injection site point?
Did you flame sterilize the needle between each innoc ?


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Offlinespacechildo
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Registered: 01/24/13
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Re: Contamination problems....HELP!!!!!! [Re: insanemike]
    #21736826 - 05/29/15 01:09 PM (8 years, 7 months ago)



no dry verm barrier or all dry verm barrier?


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InvisiblePFFFT


Registered: 08/31/09
Posts: 621
Loc: Velvet Underground
Re: Contamination problems....HELP!!!!!! [Re: spacechildo]
    #21736901 - 05/29/15 01:38 PM (8 years, 7 months ago)

Quote:

spacechildo said:


no dry verm barrier or all dry verm barrier?



:hmm::feelsbadman:


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OfflineLawnboy1185
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Re: Contamination problems....HELP!!!!!! [Re: PFFFT]
    #21736916 - 05/29/15 01:46 PM (8 years, 7 months ago)

Flamed tip every 3rd jar after the first.  And there is a 1/4 inch of dry Verm.  Right at the start of the thread.  Followed 2:1:1.


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OfflineLawnboy1185
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736925 - 05/29/15 01:49 PM (8 years, 7 months ago)

The inside of the jar became very wet with condensation but the temp never rose above 85 as far as I know.


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Offlinespacechildo
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736939 - 05/29/15 01:53 PM (8 years, 7 months ago)

Quote:

Lawnboy1185 said:
PF tek....directions to a T




Quote:

Lawnboy1185 said:
Flamed tip every 3rd jar after the first.




hwell... www.mushroomvideos.com try follow that to a T and I'm sure you'll have some success!

also I fail to see any brf in your verm there..it just doesnt look right to me.


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OfflineSyntheticwords
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Re: Contamination problems....HELP!!!!!! [Re: spacechildo]
    #21736972 - 05/29/15 02:01 PM (8 years, 7 months ago)

I think the operation is FUBAR SNAFU
I would crush cakes bury them outside
And try again


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OfflineTFS
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Registered: 05/14/15
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Loc: Canada
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21743177 - 05/30/15 11:36 PM (8 years, 7 months ago)

Quote:

Lawnboy1185 said:
Flamed tip every 3rd jar after the first.  And there is a 1/4 inch of dry Verm.  Right at the start of the thread.  Followed 2:1:1.




Although new here, I've done my fair share of pf style jars.

Those 2 jars look to be toast. If I had to make an educated guess, I'd say a dirty syringe.

Some suggestions that might help you out.

1. Flame the tip between each and every jar.
2. 1/4" dry vermiculite layer isn't enough, especially if it's courser vermiculite. The original pf-tek called for a minimum of 1/2"-2/3" barrier on top.
3. Source wide mouth jars if possible instead of the tall skinny ones, they will usually colonize faster.
4. 85 is no bueno, especially if your jars are too wet. I always found keeping them between 70-75 worked best.
5. If you feel your jars are too wet, don't be afraid to cut back and or experiment a little bit on the amount of water. Try a mixing up a batch with 5%-10%-15% less water and compare, see what works best for you.
6. Don't forget to make sure you always wipe the rim before adding your dry vermiculite.
7. If in doubt, throw it out.

Don't give up dude, use another syringe and try again, you'll get it.


Edited by TFS (05/30/15 11:37 PM)


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Invisiblermshroom
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Registered: 04/16/15
Posts: 118
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21743397 - 05/31/15 12:44 AM (8 years, 7 months ago)

Is your incubation around 80, what is your water source, and are they kept out of the light while incubating?

your vermic looks a little dry in my opinion maybe try just a little more water in your tek. Either that or it is to wet and compact. also are you injecting in the middle or the outsides?


Edited by rmshroom (05/31/15 01:01 AM)


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OfflineLawnboy1185
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Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21744244 - 05/31/15 09:29 AM (8 years, 7 months ago)

All injections were on the outside of glass.  I only flamed every 3rd jar and the inoculation was done after the PC sat overnight.  They are in an open closet with just basic natural nighting when it comes in the room. 

My first grow stalled in an incubator so people on the board said to leave it in the open.  In NE the weather went from 65 to 85 real fast so the 76-83 is room temperature.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21744259 - 05/31/15 09:39 AM (8 years, 7 months ago)

Although we don't recommend temps above 80*F I'm more than sure you can still make it work. Just click on this link and you will find a new best friend.:rasta:

http://www.shroomery.org/forums/showflat.php/Number/19208976


Edited by insanemike (05/31/15 09:40 AM)


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InvisiblePFFFT


Registered: 08/31/09
Posts: 621
Loc: Velvet Underground
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21744547 - 05/31/15 11:49 AM (8 years, 7 months ago)

Quote:

Lawnboy1185 said:
All injections were on the outside of glass.  I only flamed every 3rd jar and the inoculation was done after the PC sat overnight.  They are in an open closet with just basic natural nighting when it comes in the room. 

My first grow stalled in an incubator so people on the board said to leave it in the open.  In NE the weather went from 65 to 85 real fast so the 76-83 is room temperature.




i think that was cause of contamination..

i think each newbies first mistake is  - they do not follow tek properly.. you should flame sterilize whole needle before inoculating every jar.

but :themoreyouknow: need to learn from your own mistakes.. like i did.had to pass nearly 2years of fails until i understand that first newbie rule is simple FOLLOW THE TEK. and then  BAM mushrooms!


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Invisiblermshroom
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Registered: 04/16/15
Posts: 118
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21745179 - 05/31/15 03:27 PM (8 years, 7 months ago)

They will colonize way better in complete dark. the sunlight is creating the humidity and causing them to release chemicals that say fruit now. I bet your luck will change if you keep them out of light until full colonization :cool:


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Offlinespacechildo
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Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21745188 - 05/31/15 03:28 PM (8 years, 7 months ago)

:justno:


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Invisiblemush madness
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Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21745206 - 05/31/15 03:32 PM (8 years, 7 months ago)

Quote:

rmshroom said:
They will colonize way better in complete dark. the sunlight is creating the humidity and causing them to release chemicals that say fruit now. I bet your luck will change if you keep them out of light until full colonization :cool:




Dont listen to bad completely untrue advice


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Invisiblermshroom
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Posts: 118
Re: Contamination problems....HELP!!!!!! [Re: PFFFT]
    #21745252 - 05/31/15 03:45 PM (8 years, 7 months ago)

Quote:

PFFFT said:
Quote:

Lawnboy1185 said:
All injections were on the outside of glass.  I only flamed every 3rd jar and the inoculation was done after the PC sat overnight.  They are in an open closet with just basic natural nighting when it comes in the room. 

My first grow stalled in an incubator so people on the board said to leave it in the open.  In NE the weather went from 65 to 85 real fast so the 76-83 is room temperature.




i think that was cause of contamination..

i think each newbies first mistake is  - they do not follow tek properly.. you should flame sterilize whole needle before inoculating every jar.

but :themoreyouknow: need to learn from your own mistakes.. like i did.had to pass nearly 2years of fails until i understand that first newbie rule is simple FOLLOW THE TEK. and then  BAM mushrooms!





He had a glove box. He should have been fine. I am guessing it is the way he is making his substrate or the amount of light exposure.

Do you add the water to vermic and then flour in that order? And it was every third jar so 1 of every three should at least be sterile.


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Offlinespacechildo
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Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21745277 - 05/31/15 03:50 PM (8 years, 7 months ago)

Quote:

rmshroom said:
I am guessing




stop doing that! :shakefist:


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Invisiblermshroom
The PNW
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Registered: 04/16/15
Posts: 118
Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21745294 - 05/31/15 03:55 PM (8 years, 7 months ago)

I clearly don't know anything but here are my jars in wild bird seed. This is a easy cheap effective method here is a great tutorial and linkhttps { ://www.shroomology.org/topic/6-how-to-grow-magic-mushrooms-shrooms-in-bulk/ } this guy is a badass and inspired by the shroomery.





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Invisiblermshroom
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Registered: 04/16/15
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Re: Contamination problems....HELP!!!!!! [Re: spacechildo]
    #21745305 - 05/31/15 03:56 PM (8 years, 7 months ago)

Quote:

spacechildo said:
Quote:

rmshroom said:
I am guessing




stop doing that! :shakefist:




Oh so you know the solution for a fact? I am excited to hear it.


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Offlinespacechildo
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Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21745315 - 05/31/15 04:00 PM (8 years, 7 months ago)

it has nothing to do with lights. I've offered my help but OP haven't responded to any of it yet.

also please kill the link to the off site abomination of a website you linked, that whole site makes me wanna punch my screen!

and a little tip even tho this isn't your thread some of your jars look bacterial and all of them are ready for a shake.


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Invisiblermshroom
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Registered: 04/16/15
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Re: Contamination problems....HELP!!!!!! [Re: spacechildo]
    #21745333 - 05/31/15 04:04 PM (8 years, 7 months ago)

Quote:

spacechildo said:
it has nothing to do with lights. I've offered my help but OP haven't responded to any of it yet.

also please kill the link to the off site abomination of a website you linked, that whole site makes me wanna punch my screen!

and a little tip even tho this isn't your thread some of your jars look bacterial and all of them are ready for a shake.




Is someone jealous??


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Offlinespacechildo
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Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21745344 - 05/31/15 04:06 PM (8 years, 7 months ago)

of what? I see you reply that to most people who disagree with you. I like my grains without bacteria in them :shrug:


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Invisiblermshroom
The PNW
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Registered: 04/16/15
Posts: 118
Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21745345 - 05/31/15 04:06 PM (8 years, 7 months ago)

The middle lone jar is shook to show the difference if your curious space child. And thanks for the advice you are a kind person :crazy2:


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InvisiblePFFFT


Registered: 08/31/09
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Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21745576 - 05/31/15 05:23 PM (8 years, 7 months ago)

Quote:

rmshroom said:
They will colonize way better in complete dark. the sunlight is creating the humidity and causing them to release chemicals that say fruit now. I bet your luck will change if you keep them out of light until full colonization :cool:



they will NOT colonize better in complete dark. it was discussed earlier ant proven that mycelium grows way better in ambient light than in total darkness.
do more research before posting wrong information!
http://www.shroomery.org/forums/showflat.php/Number/16991017#16991017

Quote:

They will colonize way better in complete dark. the sunlight is creating the humidity and causing them to release chemicals that say fruit now. I bet your luck will change if you keep them out of light until full colonization :cool:


:doublefacepalm:


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OfflineLawnboy1185
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Re: Contamination problems....HELP!!!!!! [Re: PFFFT]
    #21745595 - 05/31/15 05:26 PM (8 years, 7 months ago)

I made some other jars as well and these have no contaminated yet but I can see drops of water inside all of the jars.  What is causing the condensation inside?

I mixed 2 cups of verm with 1 cup of water and mixed it in real good, then added the 1 cup brf.



Why is there condensation???  My room temp fluctuated from 76-83ish...but never above that so I'm not sure why the jars are becoming wet on the inside.


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Invisiblermshroom
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21745612 - 05/31/15 05:34 PM (8 years, 7 months ago)

My apology in the light thing, but when i tried it on my first incubation I had no success. Just mold. I may have had to much indirect, idk.

And you made sure your sub was not sticking together in big clumps and you put it in the jars without packing them down so they are nice and fluff like?


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OfflineprofessorFATTYCAP
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Re: Contamination problems....HELP!!!!!! [Re: spacechildo]
    #21745790 - 05/31/15 06:41 PM (8 years, 7 months ago)

Quote:

spacechildo said:
of what? I see you reply that to most people who disagree with you. I like my grains without bacteria in them :shrug:




ha ha ha haaa me too coincidently .


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Invisiblermshroom
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Re: Contamination problems....HELP!!!!!! [Re: professorFATTYCAP]
    #21746466 - 05/31/15 09:37 PM (8 years, 7 months ago)

Yea its too bad I got over 1000 grams wet today and more to harvest in the morning. I guess thats what shitty grains do….





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OfflineMonkeyJesusFresco
am i suspended in agar?
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Registered: 10/09/12
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Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21746518 - 05/31/15 09:50 PM (8 years, 7 months ago)

:yay:

hooray! I love troll threads!

:sploosh:


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InvisiblePFFFT


Registered: 08/31/09
Posts: 621
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21747270 - 06/01/15 03:24 AM (8 years, 7 months ago)

Quote:

Lawnboy1185 said:
I made some other jars as well and these have no contaminated yet but I can see drops of water inside all of the jars.  What is causing the condensation inside?

I mixed 2 cups of verm with 1 cup of water and mixed it in real good, then added the 1 cup brf.



Why is there condensation???  My room temp fluctuated from 76-83ish...but never above that so I'm not sure why the jars are becoming wet on the inside.



it because bit high temperature and that  jumping is not helping too.

and cause that creating condensation is that inside your jar is bit warmer than inside your room..
Quote:

The temperature of the entire content of the jars will not equalize to the tub temperature.

Colonizing mycelium generates heat, so the inside of the jars will be 2 to 5 degrees warmer than the air they sit in, meaning an 86F incubator can easily have 90F jars sitting in it, which is too much and favors contaminants such as thermophilic bacteria.

80 to 83F should be considered maximum temperature for colonizing. Personally, I colonize jars on an open shelf at normal room temperature.
RR





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OfflineLawnboy1185
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Re: Contamination problems....HELP!!!!!! [Re: PFFFT]
    #21748004 - 06/01/15 11:21 AM (8 years, 7 months ago)

How can I keep the temerature down if they are sitting in room temperature that is around 83?

When I inoculated the temp was around 60, and in a few weeks its jumped up.

I started a 4th batch (posted above) and those jars are wet on the side too?!?!


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21748026 - 06/01/15 11:27 AM (8 years, 7 months ago)

Climate control. You don't have ac of any kind?


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InvisiblePFFFT


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Re: Contamination problems....HELP!!!!!! [Re: insanemike]
    #21751420 - 06/02/15 04:01 AM (8 years, 7 months ago)

maybe a incubator would help?
i've used to live in this really shitty house,where in winter my room temperature used to drop to 13~16 C. and it slowed colonization dramaticly. so i've build tub-in-tub incubator and it helped!


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OfflinePhototropism
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Re: Contamination problems....HELP!!!!!! [Re: PFFFT]
    #21751434 - 06/02/15 04:18 AM (8 years, 7 months ago)

Quote:

PFFFT said:
maybe a incubator would help?
i've used to live in this really shitty house,where in winter my room temperature used to drop to 13~16 C. and it slowed colonization dramaticly. so i've build tub-in-tub incubator and it helped!




think op wants to reduce temp


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Invisiblemustangbob3
Mad Myrmecologist
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21751435 - 06/02/15 04:18 AM (8 years, 7 months ago)

Quote:

Lawnboy1185 said:
I made some other jars as well and these have no contaminated yet but I can see drops of water inside all of the jars.  What is causing the condensation inside?

I mixed 2 cups of verm with 1 cup of water and mixed it in real good, then added the 1 cup brf.



Why is there condensation???  My room temp fluctuated from 76-83ish...but never above that so I'm not sure why the jars are becoming wet on the inside.





condensation is caused by a temperature difference from out side the jar compared with in side. the moisture condenses on the cooler glass... has nothing to do with the amount of water contained :thumbup:


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InvisiblePFFFT


Registered: 08/31/09
Posts: 621
Loc: Velvet Underground
Re: Contamination problems....HELP!!!!!! [Re: mustangbob3]
    #21751596 - 06/02/15 06:54 AM (8 years, 7 months ago)

Quote:

Phototropism said:
Quote:

PFFFT said:
maybe a incubator would help?
i've used to live in this really shitty house,where in winter my room temperature used to drop to 13~16 C. and it slowed colonization dramaticly. so i've build tub-in-tub incubator and it helped!




think op wants to reduce temp



you can set incubator's thermostat to lower temps than room temperature is.and incubate jars in it.

didn't tried it tho. just an option:shrug:



Quote:

mustangbob3 said:
Quote:

Lawnboy1185 said:
I made some other jars as well and these have no contaminated yet but I can see drops of water inside all of the jars.  What is causing the condensation inside?

I mixed 2 cups of verm with 1 cup of water and mixed it in real good, then added the 1 cup brf.



Why is there condensation???  My room temp fluctuated from 76-83ish...but never above that so I'm not sure why the jars are becoming wet on the inside.





condensation is caused by a temperature difference from out side the jar compared with in side. the moisture condenses on the cooler glass... has nothing to do with the amount of water contained :thumbup:



i think OP just needs to read more and do more search in forums. EVERYTHING he needs is in cultivation forum. although just making a thead and waiting to answers is way easier..


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OfflineSlimeySlugNug
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Registered: 05/28/15
Posts: 29
Loc: Negril Jamaica
Last seen: 6 years, 7 months
Re: Contamination problems....HELP!!!!!! [Re: PFFFT]
    #21753086 - 06/02/15 02:33 PM (8 years, 7 months ago)

. EVERYTHING he needs is in cultivation forum. although just making a thead and waiting to answers is way easier..




It also never hurts to get feedback from his own specified thread... Everyone is hear for two reasons... To teach or to learn, even if you are one of the teacher's, some people need a little extra clarification and understanding before they completely comprehend a tek. Too many people around the internet are straight up trolls who sit and wait for someone to post something with a mistake and they all jump all over them... Lets not be shroomery trolls lollol


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OfflineMonkeyJesusFresco
am i suspended in agar?
Male


Registered: 10/09/12
Posts: 3,306
Loc: South East USA Flag
Last seen: 7 hours, 6 minutes
Re: Contamination problems....HELP!!!!!! [Re: SlimeySlugNug]
    #21753113 - 06/02/15 02:40 PM (8 years, 7 months ago)

OP misplled "HALP!!!!!!"


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