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OfflineLawnboy1185
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Registered: 03/30/15
Posts: 119
Last seen: 7 years, 3 months
Contamination problems....HELP!!!!!!
    #21736596 - 05/29/15 12:11 PM (8 years, 7 months ago)

first grow......3rd attempt.

10 jars are almost 4 weeks in and today I noticed contamination.  It looks like rat shit...and there is condensation inside the jars?  I'm not sure how the jars got wet on the inside, but 7 out of the 10 jars have signs of mold.

Why is this so hard?!?!?!?   

PF tek....directions to a T, stored in an open closet at room temp, 72-81 degrees

HELP?!?!?!?


The only jar without contamination looks like it started fruiting before colonization was complete.


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OfflineSlimeySlugNug
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Registered: 05/28/15
Posts: 29
Loc: Negril Jamaica
Last seen: 6 years, 7 months
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736615 - 05/29/15 12:18 PM (8 years, 7 months ago)

Dang that is interesting, definitely looks like some kind of contamination


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OfflineMonkeyJesusFresco
am i suspended in agar?
Male


Registered: 10/09/12
Posts: 3,306
Loc: South East USA Flag
Last seen: 8 hours, 32 minutes
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736639 - 05/29/15 12:25 PM (8 years, 7 months ago)

Quote:

Lawnboy1185 said:

The only jar without contamination looks like it started fruiting before colonization was complete.




hey...

guess what pinning before full colonization is a sign of?


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OfflineLawnboy1185
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Registered: 03/30/15
Posts: 119
Last seen: 7 years, 3 months
Re: Contamination problems....HELP!!!!!! [Re: MonkeyJesusFresco]
    #21736672 - 05/29/15 12:31 PM (8 years, 7 months ago)

I'm all ears everyone?!


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Invisiblemush madness
absorbing everything
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Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: Contamination problems....HELP!!!!!! [Re: MonkeyJesusFresco]
    #21736679 - 05/29/15 12:33 PM (8 years, 7 months ago)

I know I know pick me lol


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InvisiblePFFFT


Registered: 08/31/09
Posts: 621
Loc: Velvet Underground
Re: Contamination problems....HELP!!!!!! [Re: SlimeySlugNug]
    #21736685 - 05/29/15 12:34 PM (8 years, 7 months ago)

yup.. all your jars are f-up:awesomenod:
that "rat shit" is bacteria IMO. and cause of it might be too wet substrate you made..

i think you need to do better at sterilization methods.molds can be from contaminated print or if you made syringe or even if you done it clean-but inoculated in open air and mold spores came in thru holes in lid ect.there is tons of ways to gget contams ,but only one to prevent it - really good sterile tech!

but do not worry- you will learn how to do it. 3rd- not last. :wink:
just try again, toss contaminated jars and try improve sterility next time!:super:


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736686 - 05/29/15 12:35 PM (8 years, 7 months ago)

What was your sterile procedure from brf prep through inoculation. Maybe there is something you over looked.


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OfflineLawnboy1185
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Registered: 03/30/15
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Last seen: 7 years, 3 months
Re: Contamination problems....HELP!!!!!! [Re: insanemike]
    #21736728 - 05/29/15 12:44 PM (8 years, 7 months ago)

PC for 90 minutes, let sit over night.  Made a glove box and used rubber gloves and a long sleeve shirt.  Sprayed the inside of the glove box and the area of my kitchen with 70% alcohol.

I made 1 quick transfer from the pc to the glove box.  And removed the foil before knock up inside the glove box.  After I was done innoculating I let them sit in the glove box for 4 or 5 minutes, then transferred into the original jar box and placed on a top shelf of an open closet.


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InvisiblePFFFT


Registered: 08/31/09
Posts: 621
Loc: Velvet Underground
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736754 - 05/29/15 12:51 PM (8 years, 7 months ago)

contaminated syringe ? :shrug:


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Contamination problems....HELP!!!!!! [Re: PFFFT]
    #21736778 - 05/29/15 12:56 PM (8 years, 7 months ago)

Quote:

PFFFT said:
contaminated syringe ? :shrug:




That's what I was thinking also.

Do not use a glovebox. Use this
http://www.shroomery.org/forums/showflat.php/Number/20048771

lower your contamination rate with this.
http://www.shroomery.org/forums/showflat.php/Number/19208976

and speed up your colonization times with this.
http://www.shroomery.org/forums/showflat.php/Number/20429745/page/1


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OfflineSyntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 9 months
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736818 - 05/29/15 01:07 PM (8 years, 7 months ago)

Did you alcohol the injection site point?
Did you flame sterilize the needle between each innoc ?


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: Contamination problems....HELP!!!!!! [Re: insanemike]
    #21736826 - 05/29/15 01:09 PM (8 years, 7 months ago)



no dry verm barrier or all dry verm barrier?


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InvisiblePFFFT


Registered: 08/31/09
Posts: 621
Loc: Velvet Underground
Re: Contamination problems....HELP!!!!!! [Re: spacechildo]
    #21736901 - 05/29/15 01:38 PM (8 years, 7 months ago)

Quote:

spacechildo said:


no dry verm barrier or all dry verm barrier?



:hmm::feelsbadman:


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OfflineLawnboy1185
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Registered: 03/30/15
Posts: 119
Last seen: 7 years, 3 months
Re: Contamination problems....HELP!!!!!! [Re: PFFFT]
    #21736916 - 05/29/15 01:46 PM (8 years, 7 months ago)

Flamed tip every 3rd jar after the first.  And there is a 1/4 inch of dry Verm.  Right at the start of the thread.  Followed 2:1:1.


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OfflineLawnboy1185
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Registered: 03/30/15
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736925 - 05/29/15 01:49 PM (8 years, 7 months ago)

The inside of the jar became very wet with condensation but the temp never rose above 85 as far as I know.


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
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Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21736939 - 05/29/15 01:53 PM (8 years, 7 months ago)

Quote:

Lawnboy1185 said:
PF tek....directions to a T




Quote:

Lawnboy1185 said:
Flamed tip every 3rd jar after the first.




hwell... www.mushroomvideos.com try follow that to a T and I'm sure you'll have some success!

also I fail to see any brf in your verm there..it just doesnt look right to me.


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OfflineSyntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 9 months
Re: Contamination problems....HELP!!!!!! [Re: spacechildo]
    #21736972 - 05/29/15 02:01 PM (8 years, 7 months ago)

I think the operation is FUBAR SNAFU
I would crush cakes bury them outside
And try again


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OfflineTFS
Stranger

Registered: 05/14/15
Posts: 7
Loc: Canada
Last seen: 8 years, 6 months
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21743177 - 05/30/15 11:36 PM (8 years, 7 months ago)

Quote:

Lawnboy1185 said:
Flamed tip every 3rd jar after the first.  And there is a 1/4 inch of dry Verm.  Right at the start of the thread.  Followed 2:1:1.




Although new here, I've done my fair share of pf style jars.

Those 2 jars look to be toast. If I had to make an educated guess, I'd say a dirty syringe.

Some suggestions that might help you out.

1. Flame the tip between each and every jar.
2. 1/4" dry vermiculite layer isn't enough, especially if it's courser vermiculite. The original pf-tek called for a minimum of 1/2"-2/3" barrier on top.
3. Source wide mouth jars if possible instead of the tall skinny ones, they will usually colonize faster.
4. 85 is no bueno, especially if your jars are too wet. I always found keeping them between 70-75 worked best.
5. If you feel your jars are too wet, don't be afraid to cut back and or experiment a little bit on the amount of water. Try a mixing up a batch with 5%-10%-15% less water and compare, see what works best for you.
6. Don't forget to make sure you always wipe the rim before adding your dry vermiculite.
7. If in doubt, throw it out.

Don't give up dude, use another syringe and try again, you'll get it.


Edited by TFS (05/30/15 11:37 PM)


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Invisiblermshroom
The PNW
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Registered: 04/16/15
Posts: 118
Re: Contamination problems....HELP!!!!!! [Re: Lawnboy1185]
    #21743397 - 05/31/15 12:44 AM (8 years, 7 months ago)

Is your incubation around 80, what is your water source, and are they kept out of the light while incubating?

your vermic looks a little dry in my opinion maybe try just a little more water in your tek. Either that or it is to wet and compact. also are you injecting in the middle or the outsides?


Edited by rmshroom (05/31/15 01:01 AM)


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OfflineLawnboy1185
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Registered: 03/30/15
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Last seen: 7 years, 3 months
Re: Contamination problems....HELP!!!!!! [Re: rmshroom]
    #21744244 - 05/31/15 09:29 AM (8 years, 7 months ago)

All injections were on the outside of glass.  I only flamed every 3rd jar and the inoculation was done after the PC sat overnight.  They are in an open closet with just basic natural nighting when it comes in the room. 

My first grow stalled in an incubator so people on the board said to leave it in the open.  In NE the weather went from 65 to 85 real fast so the 76-83 is room temperature.


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