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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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How I overcome heavy room contamination!
#21736430 - 05/29/15 11:25 AM (8 years, 7 months ago) |
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To preface, it's been a while since I've posted. However, I went through a real bad trich contamination that left me running spawn with 100% success and 0-5% success after spawning to tubs with damion 50/50 bucket tech.
So, if you have an issue where your fruiting area has contaminated everything, and no matter how well you clean your tubs/buckets, you still get the mean green guarenteed, check this out! http://www.zoro.com/mold-armor-moldmildew-stain-remover-32-oz-fg502/i/G3535357/
coat the inside and outside of your tubs/buckets and let sit overnight. Then wash off the cleaner well, let air dry. Then use some clorox wipes to clean any extra debris before you spawn to bulk. I still use plenty of disinfectant to keep things clear as well throughout the process.
All said and done, I was back on track with 100% success and zero contamination!!
Hope this helps anyone struggling with the same issue I had :-)
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 8 hours, 17 minutes
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21736482 - 05/29/15 11:40 AM (8 years, 7 months ago) |
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I'm just not very convinced that "room contamination" is a real concern; sounds like you had contaminated spawn, but whatever 
I got clean spawn and bulks, and there's like...trich all over my fucking place, house plants in my 'lab', fuck'n dirty carpet everywhere...all my furniture, including the table I use for my still-air-box was in storage, next to a swamp, and came with its own mold contamination (don't know what it was, but it was green and white, and in such a quantity I almost gave up the hobby on the spot)...I just...ya know? I'm not sold on "room contamination" being a thing 
but I guess it could be... I know sterile technique is a thing, that might account for my lack of contamination...I dunno
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
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Quote:
MonkeyJesusFresco said: I'm just not very convinced that "room contamination" is a real concern; sounds like you had contaminated spawn, but whatever 
I got clean spawn and bulks, and there's like...trich all over my fucking place, house plants in my 'lab', fuck'n dirty carpet everywhere...all my furniture, including the table I use for my still-air-box was in storage, next to a swamp, and came with its own mold contamination (don't know what it was, but it was green and white, and in such a quantity I almost gave up the hobby on the spot)...I just...ya know? I'm not sold on "room contamination" being a thing 
but I guess it could be... I know sterile technique is a thing, that might account for my lack of contamination...I dunno 
 i live in an old house man, no problem with contams check your technique, are u properly pasteurizing ur substrate?
-------------------- SPREAD THE SPORES
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: How I overcome heavy room contamination! [Re: Aero]
#21736590 - 05/29/15 12:08 PM (8 years, 7 months ago) |
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: How I overcome heavy room contamination! [Re: PirateSwazey]
#21737952 - 05/29/15 06:23 PM (8 years, 7 months ago) |
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I live in an old 70s farm house with dogs lol. It's also small so I do all my SAB work in the kitchen or living room. They say a walk outside gets you covered in more trich than a contamination inside. Working with clean cultures and clean spawn is what gave me my best success rates
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
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Re: How I overcome heavy room contamination! [Re: Mad Season]
#21738225 - 05/29/15 07:22 PM (8 years, 7 months ago) |
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I know Ive read RR say he used to run his flowhood in his bedroom which is carpeted and his dog sleeps in there
I have noticed a higher rate of contamination in subsequent grows after something happens (like I didnt notice a tub in the stack has trich). Right now I am battling some other sort of green mold that seems to start off green instead of white.. I had a tub with it in my grow room and I still see it every couple of grows
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 Amateur Mycologists United AMU Q&A
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,066
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Re: How I overcome heavy room contamination! [Re: mushpunx]
#21739109 - 05/29/15 10:53 PM (8 years, 7 months ago) |
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Shmuvbox:
http://www.shroomery.org/forums/showflat.php/Number/11353633
Proper Bucket Pasteurization: http://www.shroomery.org/forums/showflat.php/Number/19907089/fpart/1/vc/1
I've had my own bouts with contamination. I think anyone who does this for more than a few months eventuallys meets the mean green. I just wipe tubs down with alcohol between runs. Practice good technique and I've gifted with quick, smooth hands.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,066
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Re: How I overcome heavy room contamination! [Re: elasticaltiger]
#21739114 - 05/29/15 10:54 PM (8 years, 7 months ago) |
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I am curious about that chemical though. If it improves success then why not?
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 8 hours, 17 minutes
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Re: How I overcome heavy room contamination! [Re: elasticaltiger]
#21739159 - 05/29/15 11:03 PM (8 years, 7 months ago) |
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,066
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Quote:
MonkeyJesusFresco said:

OMG I love xkcd. This one is my favorite to share here for when n00bs ask about using bleach and alcohol.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: How I overcome heavy room contamination! [Re: elasticaltiger]
#21752207 - 06/02/15 11:01 AM (8 years, 7 months ago) |
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Just to followup, my spawn was never contaminated...
I had 100% success with spawn runs in jars and bags... Please don't take my years not on the forums for me not knowing my process. I work with a 2X4 flowhood (which had a tear in it a few months ago, fixed with silicon). But I could tell my 95-100% contams were coming in after I spawned to bulk, right before fruiting it during fruiting. I've even built a secret area for my tubs ;-)
So long story short, i'm nothing close to being new to this, but thanks for the concern. My contams were due to getting trich badly before I figured out about the tear in my flowhood. The tubs all stayed contaminated, even after regular cleaning with bleach etc.
So the reason for my post, was to inform those who get a real bad trich contamination in their grow area to try the mold killer above! It works fantastic, and since then, i've not changed my practice and see 100% success 
-SHP
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 19 hours, 20 minutes
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21752825 - 06/02/15 01:30 PM (8 years, 7 months ago) |
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Quote:
slapphappypill said: Just to followup, my spawn was never contaminated...
I had 100% success with spawn runs in jars and bags... Please don't take my years not on the forums for me not knowing my process. I work with a 2X4 flowhood (which had a tear in it a few months ago, fixed with silicon). But I could tell my 95-100% contams were coming in after I spawned to bulk, right before fruiting it during fruiting. I've even built a secret area for my tubs ;-)
So long story short, i'm nothing close to being new to this, but thanks for the concern. My contams were due to getting trich badly before I figured out about the tear in my flowhood. The tubs all stayed contaminated, even after regular cleaning with bleach etc.
So the reason for my post, was to inform those who get a real bad trich contamination in their grow area to try the mold killer above! It works fantastic, and since then, i've not changed my practice and see 100% success 
-SHP

Of course Trichoderma has to be present for it to contaminate something. However I've noticed bacteria in the spawn has a major effect on whether the Trichoderma once present is able to grow on the substrate or not. So contaminated spawn doesn't just mean the Trichoderma itself being in the spawn even though that's what you were running into.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
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Re: How I overcome heavy room contamination! [Re: Kizzle]
#21754146 - 06/02/15 06:28 PM (8 years, 7 months ago) |
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Kizzle - what sort of advice would you give to those of us who are still struggling with losing tubs to trich?
I mean I do the best I can, I lysol my hood then run it for a bit, shower/clean up fresh scrubs tyvek,gloves, mask... blow torch my tools wash anything that goes in front of my hood down with ISO, I try to use proper hand motions
Despite my best efforts at proper sterile tek and culture work I still end up losing many tubs to trich. Is there anything else I should be doing, maybe environment wise?
I have to spawn way more tubs than I need to to ensure nice harvests.
Just open to sugguestions!
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 Amateur Mycologists United AMU Q&A
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21754187 - 06/02/15 06:37 PM (8 years, 7 months ago) |
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"But I could tell my 95-100% contams were coming in after I spawned to bulk, right before fruiting it during fruiting"
This right here sounds like its your spawn man. You wont see trich in your spawn unless you are lucky and it goes green in the jar. Quite often my masters will look/smell/behave fine otherwise, same with reciving jars from G2G, my tubs will colonize just fine.
The trich will show up once you put your tubs into fruiting conditions, you are in effect fruiting the trich as well. This is why it is so difficult to detect in your spawn.
Good rule of thumb is if your tubs are going green before or during first flush, then it is from contaminated spawn.... really you cant say for sure your spawn is clean untill you fruit your sub.
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 Amateur Mycologists United AMU Q&A
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: How I overcome heavy room contamination! [Re: mushpunx]
#21754190 - 06/02/15 06:38 PM (8 years, 7 months ago) |
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Yes oh mighty kizzle please do share. I'm hoping using sleeves in my bags have solved my problem but if not I need your wisdom as well.
Mush, are you following the rule of "first air"? Sure your hood is built right and laminar?
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
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Re: How I overcome heavy room contamination! [Re: Ghatti]
#21754251 - 06/02/15 06:48 PM (8 years, 7 months ago) |
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Quote:
Ghatti said: Yes oh mighty kizzle please do share. I'm hoping using sleeves in my bags have solved my problem but if not I need your wisdom as well.
Mush, are you following the rule of "first air"? Sure your hood is built right and laminar?

Yea man built her myself I test the flow every once in a while and I test it by leaving blank plates open for a while. It seems solid
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 Amateur Mycologists United AMU Q&A
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: How I overcome heavy room contamination! [Re: mushpunx]
#21754273 - 06/02/15 06:52 PM (8 years, 7 months ago) |
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Dunno then. Is your agar work clean?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: How I overcome heavy room contamination! [Re: Ghatti]
#21754718 - 06/02/15 08:31 PM (8 years, 7 months ago) |
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holy lots of my old favorite posters here, nice to see ya slap/monkey/pirate/tiger/kizzle/areoghattti lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: How I overcome heavy room contamination! [Re: cronicr]
#21754751 - 06/02/15 08:40 PM (8 years, 7 months ago) |
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Is bl still around? Or blojo?
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 8 hours, 17 minutes
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Re: How I overcome heavy room contamination! [Re: Ghatti]
#21754781 - 06/02/15 08:47 PM (8 years, 7 months ago) |
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cron!
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
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Re: How I overcome heavy room contamination! [Re: Ghatti]
#21756112 - 06/03/15 05:04 AM (8 years, 7 months ago) |
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Quote:
Ghatti said: Dunno then. Is your agar work clean?
Its gorgeous! Thats the one thing I do best
BL is still around yea
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 Amateur Mycologists United AMU Q&A
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 19 hours, 20 minutes
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Re: How I overcome heavy room contamination! [Re: mushpunx]
#21756289 - 06/03/15 06:50 AM (8 years, 7 months ago) |
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Quote:
Yes oh mighty kizzle please do share. I'm hoping using sleeves in my bags have solved my problem but if not I need your wisdom as well.
If you're not already, transfer your cultures to antibiotic agar at least once before using even if they look clean. For a long time I would get these Trich batches popping up that I could link to a certain culture and I thought for sure the Trichoderma was hiding in the culture. Turns out it was actually bacteria hiding in the culture, presumably the whole time. I erred as I'm sure others have by thinking if I shook up my spawn and it recovered then it should be fine, in terms of bacteria anyway. What I failed to consider is how long it was taking them to recover. The bacteria jars were taking several days to recover completely which is long enough Trichoderma spores to germinate and take over.
Anyhow, that's my most recent discovery. It's probably worth noting that excessive substrate moisture or severe lack of GE can also slow the recovery of the spawn after spawning.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
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Re: How I overcome heavy room contamination! [Re: Kizzle]
#21756413 - 06/03/15 07:46 AM (8 years, 7 months ago) |
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So like when I want to pull a slant out of the fridge I should grow it out on an antibiotic plate before I put to grains
This is an awesome little tid bit I am going to start doing this ASAP. This could be where my problems are coming from. I clean my cultures as best as I know how but I know how well these tricky contams hide.
Ive never worked with anti biotics and I usually mix my own agar, do you buy the medicine and mix it in yourself/ have a good recipe to recomend or do you use pre mix powder?
Thx
--------------------
 Amateur Mycologists United AMU Q&A
Edited by mushpunx (06/03/15 07:49 AM)
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 8 hours, 17 minutes
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Re: How I overcome heavy room contamination! [Re: mushpunx]
#21756474 - 06/03/15 08:13 AM (8 years, 7 months ago) |
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Quote:
Kizzle said:
Quote:
slapphappypill said: Just to followup, my spawn was never contaminated...
I had 100% success with spawn runs in jars and bags... Please don't take my years not on the forums for me not knowing my process. I work with a 2X4 flowhood (which had a tear in it a few months ago, fixed with silicon). But I could tell my 95-100% contams were coming in after I spawned to bulk, right before fruiting it during fruiting. I've even built a secret area for my tubs ;-)
So long story short, i'm nothing close to being new to this, but thanks for the concern. My contams were due to getting trich badly before I figured out about the tear in my flowhood. The tubs all stayed contaminated, even after regular cleaning with bleach etc.
So the reason for my post, was to inform those who get a real bad trich contamination in their grow area to try the mold killer above! It works fantastic, and since then, i've not changed my practice and see 100% success 
-SHP

Of course Trichoderma has to be present for it to contaminate something. However I've noticed bacteria in the spawn has a major effect on whether the Trichoderma once present is able to grow on the substrate or not. So contaminated spawn doesn't just mean the Trichoderma itself being in the spawn even though that's what you were running into.
My spawn was clean, very clean without any bacteria. I could grow from the bags or jars if I wanted to... However the trich spores were already in my tubs, and bad enough that bleach didn't solve my issue. Only until I used the mold killer in the OP, that I was able to get my tubs to flush without contams.
I have noticed however, that the trich is still very much a problem, as I'm losing tubs still after the first dunk and waiting on 2nd flush. I'm cutting my dunk time down to 2 hours from 4, and running fresh cold water through twice during those 2 hours. Seems to be helping get to 2nd flush for now, and we'll see about 3rd flushes haha!!!
as a point of reference, I'm used to getting 4-7 flushes from my tubs... So being able to get back to at least 3-4 flushes will make me very happy!!! I can spot bacteria in my cultures pretty easily... so I clean them a few times before I use a culture to start my spawn runs.
Thanks all for stopping by my thread 
And Chronicr!!! Sup bro!!! Great seeing ya again!! I'm going to try to frequent more often now as i've got some more free-time again
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21757534 - 06/03/15 01:59 PM (8 years, 7 months ago) |
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Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: How I overcome heavy room contamination! [Re: Mad Season]
#21757550 - 06/03/15 02:02 PM (8 years, 7 months ago) |
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Quote:
Mad Season said: Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant 
fantastic idea!!!
I'll give that a shot once I get a new spray bottle
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21757623 - 06/03/15 02:26 PM (8 years, 7 months ago) |
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Probably not getting trich from dunking but your sub is wet enough for bacteria to breed and the trich germinates on the weak spots.
BTW, what's the active ingredient in that stuff? I couldn't find an ingredient list on the website.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: How I overcome heavy room contamination! [Re: Ghatti]
#21757636 - 06/03/15 02:29 PM (8 years, 7 months ago) |
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That's exactly what it is ghatti. I just find it easier to mist when it looks dry rather than dry it out when it's too wet
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: How I overcome heavy room contamination! [Re: Ghatti]
#21757657 - 06/03/15 02:35 PM (8 years, 7 months ago) |
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I used to dunk my tubs a day or two after harvest... to let it dry a little and let the harvest spots heal some before dunking... maybe thats part of it? I twist and pull, so sometimes I get chunks of sub that come off and tend to be the places that trich starts after a dunk.
However, I did find one tub where the trich seemed to start on a stump... so i'm not sure. Either way, I like the heavy misting idea between flushes, as that will allow me to skip the dunking step and just mist inbetween working 
As far as the mold killer Ghatti, all the bottle says is bleach free and that it has no harsh toxic fumes so indoor and small space use is ok. There is a 1-888-326-7743 number to call them for questions and comments. They could tell you i'm sure
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21757661 - 06/03/15 02:37 PM (8 years, 7 months ago) |
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So my tubs probably contamed on 2nd flush because they accidentally were dunked overnight then. I talked to the wife, and she said she forgot till the morning.
So oh well, i'll stick to misting for now, and see how my 4 hr and 2 hr dunks hold over the next week
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21757681 - 06/03/15 02:48 PM (8 years, 7 months ago) |
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wait...so did the mold killer work or not?
this is crazy.
my money is on your grow area, not your tubs. you got germinated trich fly'n around when you spawn, I guarantee it.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Does it smell like citrus?
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mushpunx
Fungus Punk



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Quote:
MonkeyJesusFresco said: http://everythingmushrooms.com/cultivation-tools/mushroom-culturing-tools-and-agar-supplies/media-antibiotic/?page_context=category&faceted_search=0
personally, the only reason I haven't purchased it yet is cause I don't necessarily NEED a whole batch of antibiotic agar plates (1L of agar)

"Gentamycin Sulfate- Autoclavable. For invitro use only. Not for ingestion. Add 1/20 gram per liter of agar media prior to sterilization. Prevents bacterial growth while stimulating mushroom mycelium. Highly recommended. Not for prolonged use. 1 pack (1/20 gram)"
Hmm it seems easy enough, I suppose you could quarter the bottle too if you only needed a small batch yea?
Thanks for putting that up Im gunna try Kizzles tip so I thimk Im gunna buy this
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slapphappypill
Enthusiast!




Registered: 11/07/10
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Quote:
MonkeyJesusFresco said: wait...so did the mold killer work or not?
this is crazy.
my money is on your grow area, not your tubs. you got germinated trich fly'n around when you spawn, I guarantee it.
The mold killer WORKS haha! I was having issues before first flush and sometimes before fruiting even while still in the tubs. The grow area has mold spores also, but getting to first flush shouldn't be an issue. I do all work in front of my 4ft flowhood, including spawning to bulk.
Once again though, I'll remind everyone, that I KNOW for a fact my cultures and spawn were solid... as I am currently using the same cultures and spawn practices with 100% success...
My only current issue was that my wife left my 2 tubs dunked overnight, instead of 4 hours haha! Those are the only two tubs I see with any trich
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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transkei
Mr



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Re: How I overcome heavy room contamination! [Re: PirateSwazey]
#21757804 - 06/03/15 03:26 PM (8 years, 7 months ago) |
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Wow, that is bad ass!
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: How I overcome heavy room contamination! [Re: mushpunx]
#21758270 - 06/03/15 05:27 PM (8 years, 7 months ago) |
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Quote:
mushpunx said: So like when I want to pull a slant out of the fridge I should grow it out on an antibiotic plate before I put to grains
This is an awesome little tid bit I am going to start doing this ASAP. This could be where my problems are coming from. I clean my cultures as best as I know how but I know how well these tricky contams hide.
Ive never worked with anti biotics and I usually mix my own agar, do you buy the medicine and mix it in yourself/ have a good recipe to recomend or do you use pre mix powder?
Thx
Just do use it for the first transfer after you make clone culture or germinate some spores.
Quote:
My spawn was clean, very clean without any bacteria. I could grow from the bags or jars if I wanted to...
Well that's kind of my whole point. I can grow from a bacteria jar too, but if I spawn one it winds up with Trich either at fruiting or growing on the side as soon the substrate pulls away enough to expose the grains there during the second flush.
Here's an example of a rogue spawn jar about 2 days after shaking at full colonization.
 At a glance you'd think it's recovering fine. No slime or smell or anything like that usually associated with bacteria.
And if you happen to notice something like this in your tubs it's pretty clear the grain had not recovered in the spot where the Trich started growing.
Edited by Kizzle (06/03/15 05:36 PM)
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Sockadin



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Re: How I overcome heavy room contamination! [Re: Kizzle]
#21758363 - 06/03/15 05:44 PM (8 years, 7 months ago) |
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Well this is a fountain of information. Just wanted to throw my 2 cents in. I think everyone has a run in with spawn busts. I currently have a massive out break that I lost all of my jars to. I made up some more petri dishes to start over and those contaminated before I ever transferred mycelium to them. The funk in my place got so bad I realized 1 thing.
The number 1 cause of all of my contamination issues recently was, LAZYNESS. I got just plain lazy when I poured my petris, and let them in the glove box over night to cool before wrapping them with syran. I never checked my LC's on agar to make sure they were clean, and I didn't transfer my original agar enough to clean out the hiding trich in them. It's all a process, and I have learned that I just get to damn lazy for this hobby. My head is all wrapped up in my aquaponics system anyway.
But yeah good looking mold blocker, does it work on bacteria?
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Kizzle
Misanthrope


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Re: How I overcome heavy room contamination! [Re: Sockadin]
#21758478 - 06/03/15 06:08 PM (8 years, 7 months ago) |
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One other thing. Let the condensation disappear completely after pouring before using agar plates if you can. Bacteria will sometime appear on a plate as very slight discoloration of the surface. In the few days it takes for the condensation to completely disappear so can actually get a good look there's enough time for the bacteria to completely cover surface and when there's no edge of a bacteria colony to be seen it's less noticeable. I know in at least one case that's how it managed to slip by 
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,066
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Re: How I overcome heavy room contamination! [Re: Mad Season]
#21758752 - 06/03/15 07:17 PM (8 years, 7 months ago) |
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Quote:
Mad Season said: Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant 

Everyone gets 5 - 8 flushes when they first start growing. Especially if they are in a newer building or a well kept older one. After your first big contamination flushes go down to two.
I get two solid flushes before I see green. I used to gasp and wonder at the people who said that they throw tubs away as soon as the second flush is over but now I see that this really has to be the standard practice.
Third flushes are hardly ever yielding the kind of weight worth waiting around for in my case. So now I toss them too. The one thing I can say holds true for me about third and later flushes is that the shrooms always seem to be significantly bigger later on.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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slapphappypill
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Re: How I overcome heavy room contamination! [Re: Sockadin]
#21761584 - 06/04/15 12:57 PM (8 years, 7 months ago) |
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Quote:
elasticaltiger said:
Quote:
Mad Season said: Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant 

Everyone gets 5 - 8 flushes when they first start growing. Especially if they are in a newer building or a well kept older one. After your first big contamination flushes go down to two.
I get two solid flushes before I see green. I used to gasp and wonder at the people who said that they throw tubs away as soon as the second flush is over but now I see that this really has to be the standard practice.
Third flushes are hardly ever yielding the kind of weight worth waiting around for in my case. So now I toss them too. The one thing I can say holds true for me about third and later flushes is that the shrooms always seem to be significantly bigger later on.
rgr that 
Quote:
Kizzle said:
Quote:
mushpunx said: So like when I want to pull a slant out of the fridge I should grow it out on an antibiotic plate before I put to grains
This is an awesome little tid bit I am going to start doing this ASAP. This could be where my problems are coming from. I clean my cultures as best as I know how but I know how well these tricky contams hide.
Ive never worked with anti biotics and I usually mix my own agar, do you buy the medicine and mix it in yourself/ have a good recipe to recomend or do you use pre mix powder?
Thx
Just do use it for the first transfer after you make clone culture or germinate some spores.
Quote:
My spawn was clean, very clean without any bacteria. I could grow from the bags or jars if I wanted to...
Well that's kind of my whole point. I can grow from a bacteria jar too, but if I spawn one it winds up with Trich either at fruiting or growing on the side as soon the substrate pulls away enough to expose the grains there during the second flush.
Here's an example of a rogue spawn jar about 2 days after shaking at full colonization.
 At a glance you'd think it's recovering fine. No slime or smell or anything like that usually associated with bacteria.
And if you happen to notice something like this in your tubs it's pretty clear the grain had not recovered in the spot where the Trich started growing.

I see where your issue was, however, the myc in my jars and bags never had issues with recovery and tub colonizing went pretty well also. I'm very convinced that since the spray worked, and that was the last thing I changed from previous attempts, that my tubs/buckets were still harboring contams even after washing.
The mold spray works even after washing etc, so it actually prevents the trich from bothering my healthy myc. I'm not saying your scenario doesn't happen, as it does quite often, but i'm saying that I tend to find very uncommon issues when dealing with mycology 
I've been through arguments with RR before where he was dead certain that he knew the issue, but my solution was the fix.
Quote:
Tetramine said: Well this is a fountain of information. Just wanted to throw my 2 cents in. I think everyone has a run in with spawn busts. I currently have a massive out break that I lost all of my jars to. I made up some more petri dishes to start over and those contaminated before I ever transferred mycelium to them. The funk in my place got so bad I realized 1 thing.
The number 1 cause of all of my contamination issues recently was, LAZYNESS. I got just plain lazy when I poured my petris, and let them in the glove box over night to cool before wrapping them with syran. I never checked my LC's on agar to make sure they were clean, and I didn't transfer my original agar enough to clean out the hiding trich in them. It's all a process, and I have learned that I just get to damn lazy for this hobby. My head is all wrapped up in my aquaponics system anyway.
But yeah good looking mold blocker, does it work on bacteria?
I think it works on almost everything.... It's a solid product for sure!!! Like I said, I soak everything I work with in it overnight, rinse it all off well with hot water, then spray with lysol before using. Worked like a charm 100% of the time now.
I've attributed my 2nd flush trich with the tubs being forgotten overnight in a dunk. So bacteria/trich was a high risk. My 2 hour/4 hour dunked tubs are strong.
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Ncogneato
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21762042 - 06/04/15 03:07 PM (8 years, 7 months ago) |
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At what point is it a good idea to replace tubs all together with new tubs?
My point here is; every time you clean tubs (even lightly wiping them down) you are creating microscopic pits and scratches that may harbor nasties that are not visible to the eye and may not be penetrated by cleaners. Also, the cleaning solutions used are merely "sanitizers" rather than "sterilizers" so getting rid of unwanted molds and bacteria from these microscopic imperfections in the tubs would conceivable become more and more difficult with every cleaning.
My experience is very limited so I ask the community. How many runs before you replace your tubs all together?
-------------------- Psilocybin.........the poor man's Cancun.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: How I overcome heavy room contamination! [Re: elasticaltiger]
#21762045 - 06/04/15 03:08 PM (8 years, 7 months ago) |
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Quote:
elasticaltiger said:
Quote:
Mad Season said: Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant 

Everyone gets 5 - 8 flushes when they first start growing. Especially if they are in a newer building or a well kept older one. After your first big contamination flushes go down to two.
I get two solid flushes before I see green. I used to gasp and wonder at the people who said that they throw tubs away as soon as the second flush is over but now I see that this really has to be the standard practice.
Third flushes are hardly ever yielding the kind of weight worth waiting around for in my case. So now I toss them too. The one thing I can say holds true for me about third and later flushes is that the shrooms always seem to be significantly bigger later on.
Haha yea man when I was first starting to grow I just couldnt figure out how other people were getting so many contams my first 10 + grows were clean, just syringe to grains no agar pretty lousy teqnique
Then after my first green tub trich is pretty frequent in my home
On tubs with real even pinsets I get 70% of my yeild im the first flush anyways then most of the rest in the secomd anyways
But I like to put them on the porch after that especially since the fruits get sooooo huge I love it!
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: How I overcome heavy room contamination! [Re: Ncogneato]
#21762053 - 06/04/15 03:10 PM (8 years, 7 months ago) |
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Quote:
Ncogneato said: At what point is it a good idea to replace tubs all together with new tubs?
My point here is; every time you clean tubs (even lightly wiping them down) you are creating microscopic pits and scratches that may harbor nasties that are not visible to the eye and may not be penetrated by cleaners. Also, the cleaning solutions used are merely "sanitizers" rather than "sterilizers" so getting rid of unwanted molds and bacteria from these microscopic imperfections in the tubs would conceivable become more and more difficult with every cleaning.
My experience is very limited so I ask the community. How many runs before you replace your tubs all together?
Nah jusy wash them out with soap and water. Your tubs are never gunna be *that* clean anyways, as long as you have clean spawn and pastutize properly it shouldnt matter.
Use your tubs untill they break
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: How I overcome heavy room contamination! [Re: mushpunx]
#21766079 - 06/05/15 02:09 PM (8 years, 7 months ago) |
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Quote:
Everyone gets 5 - 8 flushes when they first start growing. Especially if they are in a newer building or a well kept older one. After your first big contamination flushes go down to two.
Mold spores are not difficult to remove from a house. If there is an increase in contamination it's probably do to changes in methodology. Switching to bulk substrates, doing g2gs, making your own inoculant, stuff like that.
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britzman
Stranger

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Re: How I overcome heavy room contamination! [Re: Kizzle]
#21766155 - 06/05/15 02:35 PM (8 years, 7 months ago) |
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Buy an ozone generator!
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
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Re: How I overcome heavy room contamination! [Re: britzman]
#21766170 - 06/05/15 02:40 PM (8 years, 7 months ago) |
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Please don't. Ozone is very bad for you
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Kizzle
Misanthrope


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Re: How I overcome heavy room contamination! [Re: britzman]
#21766540 - 06/05/15 04:10 PM (8 years, 7 months ago) |
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 Here's something from EPA. Besides the potential health effects it states it's generally ineffective anyway. http://www.epa.gov/iaq/pubs/ozonegen.html
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britzman
Stranger

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Re: How I overcome heavy room contamination! [Re: Kizzle]
#21769033 - 06/06/15 05:46 AM (8 years, 7 months ago) |
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I use one and never got contams, i live a messy house; pets and many ppl. Just open a window after you run it and youll be safe. Its dangerous to good bacteria too thats why the epa warns about it. If you ventilate for 20 mins, no worries at all!
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slapphappypill
Enthusiast!




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Re: How I overcome heavy room contamination! [Re: britzman]
#21847237 - 06/23/15 07:08 PM (8 years, 7 months ago) |
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yeah, i'm just going to continue with my new method of not dunking after 1st flush and tossing after 2nd. Working great at about 12-14 oz per tub total
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21847257 - 06/23/15 07:12 PM (8 years, 7 months ago) |
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Quote:
slapphappypill said: yeah, i'm just going to continue with my new method of not dunking after 1st flush and tossing after 2nd. Working great at about 12-14 oz per tub total
12 to 14 dry oz per tub in 2 flushes is pretty fantastic dude.
Shouldnt need to dunk after first flush. I never dunk my tubs actually.
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