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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: How I overcome heavy room contamination! [Re: Sockadin]
#21761584 - 06/04/15 12:57 PM (8 years, 7 months ago) |
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elasticaltiger said:
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Mad Season said: Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant 

Everyone gets 5 - 8 flushes when they first start growing. Especially if they are in a newer building or a well kept older one. After your first big contamination flushes go down to two.
I get two solid flushes before I see green. I used to gasp and wonder at the people who said that they throw tubs away as soon as the second flush is over but now I see that this really has to be the standard practice.
Third flushes are hardly ever yielding the kind of weight worth waiting around for in my case. So now I toss them too. The one thing I can say holds true for me about third and later flushes is that the shrooms always seem to be significantly bigger later on.
rgr that 
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Kizzle said:
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mushpunx said: So like when I want to pull a slant out of the fridge I should grow it out on an antibiotic plate before I put to grains
This is an awesome little tid bit I am going to start doing this ASAP. This could be where my problems are coming from. I clean my cultures as best as I know how but I know how well these tricky contams hide.
Ive never worked with anti biotics and I usually mix my own agar, do you buy the medicine and mix it in yourself/ have a good recipe to recomend or do you use pre mix powder?
Thx
Just do use it for the first transfer after you make clone culture or germinate some spores.
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My spawn was clean, very clean without any bacteria. I could grow from the bags or jars if I wanted to...
Well that's kind of my whole point. I can grow from a bacteria jar too, but if I spawn one it winds up with Trich either at fruiting or growing on the side as soon the substrate pulls away enough to expose the grains there during the second flush.
Here's an example of a rogue spawn jar about 2 days after shaking at full colonization.
 At a glance you'd think it's recovering fine. No slime or smell or anything like that usually associated with bacteria.
And if you happen to notice something like this in your tubs it's pretty clear the grain had not recovered in the spot where the Trich started growing.

I see where your issue was, however, the myc in my jars and bags never had issues with recovery and tub colonizing went pretty well also. I'm very convinced that since the spray worked, and that was the last thing I changed from previous attempts, that my tubs/buckets were still harboring contams even after washing.
The mold spray works even after washing etc, so it actually prevents the trich from bothering my healthy myc. I'm not saying your scenario doesn't happen, as it does quite often, but i'm saying that I tend to find very uncommon issues when dealing with mycology 
I've been through arguments with RR before where he was dead certain that he knew the issue, but my solution was the fix.
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Tetramine said: Well this is a fountain of information. Just wanted to throw my 2 cents in. I think everyone has a run in with spawn busts. I currently have a massive out break that I lost all of my jars to. I made up some more petri dishes to start over and those contaminated before I ever transferred mycelium to them. The funk in my place got so bad I realized 1 thing.
The number 1 cause of all of my contamination issues recently was, LAZYNESS. I got just plain lazy when I poured my petris, and let them in the glove box over night to cool before wrapping them with syran. I never checked my LC's on agar to make sure they were clean, and I didn't transfer my original agar enough to clean out the hiding trich in them. It's all a process, and I have learned that I just get to damn lazy for this hobby. My head is all wrapped up in my aquaponics system anyway.
But yeah good looking mold blocker, does it work on bacteria?
I think it works on almost everything.... It's a solid product for sure!!! Like I said, I soak everything I work with in it overnight, rinse it all off well with hot water, then spray with lysol before using. Worked like a charm 100% of the time now.
I've attributed my 2nd flush trich with the tubs being forgotten overnight in a dunk. So bacteria/trich was a high risk. My 2 hour/4 hour dunked tubs are strong.
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Ncogneato
Patriot



Registered: 04/27/15
Posts: 228
Loc: Wisco
Last seen: 7 years, 11 months
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21762042 - 06/04/15 03:07 PM (8 years, 7 months ago) |
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At what point is it a good idea to replace tubs all together with new tubs?
My point here is; every time you clean tubs (even lightly wiping them down) you are creating microscopic pits and scratches that may harbor nasties that are not visible to the eye and may not be penetrated by cleaners. Also, the cleaning solutions used are merely "sanitizers" rather than "sterilizers" so getting rid of unwanted molds and bacteria from these microscopic imperfections in the tubs would conceivable become more and more difficult with every cleaning.
My experience is very limited so I ask the community. How many runs before you replace your tubs all together?
-------------------- Psilocybin.........the poor man's Cancun.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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Re: How I overcome heavy room contamination! [Re: elasticaltiger]
#21762045 - 06/04/15 03:08 PM (8 years, 7 months ago) |
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Quote:
elasticaltiger said:
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Mad Season said: Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant 

Everyone gets 5 - 8 flushes when they first start growing. Especially if they are in a newer building or a well kept older one. After your first big contamination flushes go down to two.
I get two solid flushes before I see green. I used to gasp and wonder at the people who said that they throw tubs away as soon as the second flush is over but now I see that this really has to be the standard practice.
Third flushes are hardly ever yielding the kind of weight worth waiting around for in my case. So now I toss them too. The one thing I can say holds true for me about third and later flushes is that the shrooms always seem to be significantly bigger later on.
Haha yea man when I was first starting to grow I just couldnt figure out how other people were getting so many contams my first 10 + grows were clean, just syringe to grains no agar pretty lousy teqnique
Then after my first green tub trich is pretty frequent in my home
On tubs with real even pinsets I get 70% of my yeild im the first flush anyways then most of the rest in the secomd anyways
But I like to put them on the porch after that especially since the fruits get sooooo huge I love it!
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 Amateur Mycologists United AMU Q&A
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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Re: How I overcome heavy room contamination! [Re: Ncogneato]
#21762053 - 06/04/15 03:10 PM (8 years, 7 months ago) |
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Ncogneato said: At what point is it a good idea to replace tubs all together with new tubs?
My point here is; every time you clean tubs (even lightly wiping them down) you are creating microscopic pits and scratches that may harbor nasties that are not visible to the eye and may not be penetrated by cleaners. Also, the cleaning solutions used are merely "sanitizers" rather than "sterilizers" so getting rid of unwanted molds and bacteria from these microscopic imperfections in the tubs would conceivable become more and more difficult with every cleaning.
My experience is very limited so I ask the community. How many runs before you replace your tubs all together?
Nah jusy wash them out with soap and water. Your tubs are never gunna be *that* clean anyways, as long as you have clean spawn and pastutize properly it shouldnt matter.
Use your tubs untill they break
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 Amateur Mycologists United AMU Q&A
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 8 minutes
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Re: How I overcome heavy room contamination! [Re: mushpunx]
#21766079 - 06/05/15 02:09 PM (8 years, 7 months ago) |
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Quote:
Everyone gets 5 - 8 flushes when they first start growing. Especially if they are in a newer building or a well kept older one. After your first big contamination flushes go down to two.
Mold spores are not difficult to remove from a house. If there is an increase in contamination it's probably do to changes in methodology. Switching to bulk substrates, doing g2gs, making your own inoculant, stuff like that.
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britzman
Stranger

Registered: 04/03/15
Posts: 25
Last seen: 8 years, 7 months
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Re: How I overcome heavy room contamination! [Re: Kizzle]
#21766155 - 06/05/15 02:35 PM (8 years, 7 months ago) |
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Buy an ozone generator!
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: How I overcome heavy room contamination! [Re: britzman]
#21766170 - 06/05/15 02:40 PM (8 years, 7 months ago) |
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Please don't. Ozone is very bad for you
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 8 minutes
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Re: How I overcome heavy room contamination! [Re: britzman]
#21766540 - 06/05/15 04:10 PM (8 years, 7 months ago) |
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 Here's something from EPA. Besides the potential health effects it states it's generally ineffective anyway. http://www.epa.gov/iaq/pubs/ozonegen.html
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britzman
Stranger

Registered: 04/03/15
Posts: 25
Last seen: 8 years, 7 months
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Re: How I overcome heavy room contamination! [Re: Kizzle]
#21769033 - 06/06/15 05:46 AM (8 years, 7 months ago) |
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I use one and never got contams, i live a messy house; pets and many ppl. Just open a window after you run it and youll be safe. Its dangerous to good bacteria too thats why the epa warns about it. If you ventilate for 20 mins, no worries at all!
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: How I overcome heavy room contamination! [Re: britzman]
#21847237 - 06/23/15 07:08 PM (8 years, 7 months ago) |
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yeah, i'm just going to continue with my new method of not dunking after 1st flush and tossing after 2nd. Working great at about 12-14 oz per tub total
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
#21847257 - 06/23/15 07:12 PM (8 years, 7 months ago) |
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Quote:
slapphappypill said: yeah, i'm just going to continue with my new method of not dunking after 1st flush and tossing after 2nd. Working great at about 12-14 oz per tub total
12 to 14 dry oz per tub in 2 flushes is pretty fantastic dude.
Shouldnt need to dunk after first flush. I never dunk my tubs actually.
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 Amateur Mycologists United AMU Q&A
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