Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate

Jump to first unread post Pages: < Back | 1 | 2 | 3 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: Ghatti]
    #21756112 - 06/03/15 05:04 AM (8 years, 7 months ago)

Quote:

Ghatti said:
Dunno then. Is your agar work clean?





Its gorgeous! Thats the one thing I do best

BL is still around yea


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 25 minutes
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: mushpunx]
    #21756289 - 06/03/15 06:50 AM (8 years, 7 months ago)

Quote:

Yes oh mighty kizzle please do share. I'm hoping using sleeves in my bags have solved my problem but if not I need your wisdom as well.



If you're not already, transfer your cultures to antibiotic agar at least once before using even if they look clean. For a long time I would get these Trich batches popping up that I could link to a certain culture and I thought for sure the Trichoderma was hiding in the culture. Turns out it was actually bacteria hiding in the culture, presumably the whole time. I erred as I'm sure others have by thinking if I shook up my spawn and it recovered then it should be fine, in terms of bacteria anyway. What I failed to consider is how long it was taking them to recover. The bacteria jars were taking several days to recover completely which is long enough Trichoderma spores to germinate and take over.

Anyhow, that's my most recent discovery. It's probably worth noting that excessive substrate moisture or severe lack of GE can also slow the recovery of the spawn after spawning.


--------------------


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: Kizzle]
    #21756413 - 06/03/15 07:46 AM (8 years, 7 months ago)

So like when I want to pull a slant out of the fridge I should grow it out on an antibiotic plate before I put to grains

This is an awesome little tid bit I am going to start doing this ASAP.

This could be where my problems are coming from. I clean my cultures as  best as I know how but I know how well these tricky contams hide.

Ive never worked with anti biotics and I usually mix my own agar, do you buy the medicine and mix it in yourself/ have a good recipe to recomend or do you use pre mix powder?

Thx


--------------------

Amateur Mycologists United
AMU Q&A


Edited by mushpunx (06/03/15 07:49 AM)


Extras: Filter Print Post Top
OfflineMonkeyJesusFresco
am i suspended in agar?
Male


Registered: 10/09/12
Posts: 3,306
Loc: South East USA Flag
Last seen: 5 hours, 22 minutes
Re: How I overcome heavy room contamination! [Re: mushpunx]
    #21756474 - 06/03/15 08:13 AM (8 years, 7 months ago)

http://everythingmushrooms.com/cultivation-tools/mushroom-culturing-tools-and-agar-supplies/media-antibiotic/?page_context=category&faceted_search=0

personally, the only reason I haven't purchased it yet is cause I don't necessarily NEED a whole batch of antibiotic agar plates (1L of agar)

:shrug:


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: How I overcome heavy room contamination! [Re: MonkeyJesusFresco]
    #21757513 - 06/03/15 01:55 PM (8 years, 7 months ago)

Quote:

Kizzle said:
Quote:

slapphappypill said:
Just to followup, my spawn was never contaminated...

I had 100% success with spawn runs in jars and bags... Please don't take my years not on the forums for me not knowing my process. I work with a 2X4 flowhood (which had a tear in it a few months ago, fixed with silicon). But I could tell my 95-100% contams were coming in after I spawned to bulk, right before fruiting it during fruiting. I've even built a secret area for my tubs ;-)

So long story short, i'm nothing close to being new to this, but thanks for the concern. My contams were due to getting trich badly before I figured out about the tear in my flowhood. The tubs all stayed contaminated, even after regular cleaning with bleach etc.

So the reason for my post, was to inform those who get a real bad trich contamination in their grow area to try the mold killer above! It works fantastic, and since then, i've not changed my practice and see 100% success :jah:

-SHP






Of course Trichoderma has to be present for it to contaminate something. However I've noticed bacteria in the spawn has a major effect on whether the Trichoderma once present is able to grow on the substrate or not. So contaminated spawn doesn't just mean the Trichoderma itself being in the spawn even though that's what you were running into.





My spawn was clean, very clean without any bacteria. I could grow from the bags or jars if I wanted to... However the trich spores were already in my tubs, and bad enough that bleach didn't solve my issue. Only until I used the mold killer in the OP, that I was able to get my tubs to flush without contams.

I have noticed however, that the trich is still very much a problem, as I'm losing tubs still after the first dunk and waiting on 2nd flush. I'm cutting my dunk time down to 2 hours from 4, and running fresh cold water through twice during those 2 hours. Seems to be helping get to 2nd flush for now, and we'll see about 3rd flushes haha!!!

as a point of reference, I'm used to getting 4-7 flushes from my tubs... So being able to get back to at least 3-4 flushes will make me very happy!!! I can spot bacteria in my cultures pretty easily... so I clean them a few times before I use a culture to start my spawn runs.

Thanks all for stopping by my thread :datass:




And Chronicr!!! Sup bro!!! Great seeing ya again!! I'm going to try to frequent more often now as i've got some more free-time again :bender:


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: slapphappypill]
    #21757534 - 06/03/15 01:59 PM (8 years, 7 months ago)

Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: How I overcome heavy room contamination! [Re: Mad Season]
    #21757550 - 06/03/15 02:02 PM (8 years, 7 months ago)

Quote:

Mad Season said:
Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant :smile:




fantastic idea!!!

I'll give that a shot once I get a new spray bottle :hamsterdance:


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: How I overcome heavy room contamination! [Re: slapphappypill]
    #21757623 - 06/03/15 02:26 PM (8 years, 7 months ago)

Probably not getting trich from dunking but your sub is wet enough for bacteria to breed and the trich germinates on the weak spots.


BTW, what's the active ingredient in that stuff? I couldn't find an ingredient list on the website.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: Ghatti]
    #21757636 - 06/03/15 02:29 PM (8 years, 7 months ago)

That's exactly what it is ghatti. I just find it easier to mist when it looks dry rather than dry it out when it's too wet :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: How I overcome heavy room contamination! [Re: Ghatti]
    #21757657 - 06/03/15 02:35 PM (8 years, 7 months ago)

I used to dunk my tubs a day or two after harvest... to let it dry a little and let the harvest spots heal some before dunking... maybe thats part of it? I twist and pull, so sometimes I get chunks of sub that come off and tend to be the places that trich starts after a dunk.

However, I did find one tub where the trich seemed to start on a stump... so i'm not sure. Either way, I like the heavy misting idea between flushes, as that will allow me to skip the dunking step and just mist inbetween working :jah:


As far as the mold killer Ghatti, all the bottle says is bleach free and that it has no harsh toxic fumes so indoor and small space use is ok. There is a 1-888-326-7743 number to call them for questions and comments. They could tell you i'm sure :bigjoint:


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: How I overcome heavy room contamination! [Re: slapphappypill]
    #21757661 - 06/03/15 02:37 PM (8 years, 7 months ago)

So my tubs probably contamed on 2nd flush because they accidentally were dunked overnight then. I talked to the wife, and she said she forgot till the morning.

So oh well, i'll stick to misting for now, and see how my 4 hr and 2 hr dunks hold over the next week :jah:


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
OfflineMonkeyJesusFresco
am i suspended in agar?
Male


Registered: 10/09/12
Posts: 3,306
Loc: South East USA Flag
Last seen: 5 hours, 22 minutes
Re: How I overcome heavy room contamination! [Re: slapphappypill]
    #21757681 - 06/03/15 02:48 PM (8 years, 7 months ago)

wait...so did the mold killer work or not?

this is crazy.

my money is on your grow area, not your tubs. you got germinated trich fly'n around when you spawn, I guarantee it.


Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: How I overcome heavy room contamination! [Re: MonkeyJesusFresco]
    #21757686 - 06/03/15 02:50 PM (8 years, 7 months ago)

Does it smell like citrus?


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: MonkeyJesusFresco]
    #21757717 - 06/03/15 03:02 PM (8 years, 7 months ago)

Quote:

MonkeyJesusFresco said:
http://everythingmushrooms.com/cultivation-tools/mushroom-culturing-tools-and-agar-supplies/media-antibiotic/?page_context=category&faceted_search=0

personally, the only reason I haven't purchased it yet is cause I don't necessarily NEED a whole batch of antibiotic agar plates (1L of agar)

:shrug:





"Gentamycin Sulfate- Autoclavable. For invitro use only. Not for ingestion. Add 1/20 gram per liter of agar media prior to sterilization. Prevents bacterial growth while stimulating mushroom mycelium. Highly recommended. Not for prolonged use. 1 pack (1/20 gram)"


  Hmm it seems easy enough, I suppose you could quarter the bottle too if you only needed a small batch yea?

Thanks for putting that up Im gunna try Kizzles tip so I thimk Im gunna buy this


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Invisibleslapphappypill
Enthusiast!
 User Gallery


Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: How I overcome heavy room contamination! [Re: MonkeyJesusFresco]
    #21757783 - 06/03/15 03:21 PM (8 years, 7 months ago)

Quote:

MonkeyJesusFresco said:
wait...so did the mold killer work or not?

this is crazy.

my money is on your grow area, not your tubs. you got germinated trich fly'n around when you spawn, I guarantee it.





The mold killer WORKS haha! I was having issues before first flush and sometimes before fruiting even while still in the tubs. The grow area has mold spores also, but getting to first flush shouldn't be an issue. I do all work in front of my 4ft flowhood, including spawning to bulk.



Once again though, I'll remind everyone, that I KNOW for a fact my cultures and spawn were solid... as I am currently using the same cultures and spawn practices with 100% success...

My only current issue was that my wife left my 2 tubs dunked overnight, instead of 4 hours haha! Those are the only two tubs I see with any trich


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Extras: Filter Print Post Top
Offlinetranskei
Mr
Male User Gallery


Registered: 05/24/07
Posts: 218
Loc: Texas Flag
Last seen: 1 year, 10 months
Re: How I overcome heavy room contamination! [Re: PirateSwazey]
    #21757804 - 06/03/15 03:26 PM (8 years, 7 months ago)

Wow, that is bad ass!


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 25 minutes
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: mushpunx]
    #21758270 - 06/03/15 05:27 PM (8 years, 7 months ago)

Quote:

mushpunx said:
So like when I want to pull a slant out of the fridge I should grow it out on an antibiotic plate before I put to grains

This is an awesome little tid bit I am going to start doing this ASAP.

This could be where my problems are coming from. I clean my cultures as  best as I know how but I know how well these tricky contams hide.

Ive never worked with anti biotics and I usually mix my own agar, do you buy the medicine and mix it in yourself/ have a good recipe to recomend or do you use pre mix powder?

Thx



Just do use it for the first transfer after you make clone culture or germinate some spores.


Quote:

My spawn was clean, very clean without any bacteria. I could grow from the bags or jars if I wanted to...



Well that's kind of my whole point. I can grow from a bacteria jar too, but if I spawn one it winds up with Trich either at fruiting or growing on the side as soon the substrate pulls away enough to expose the grains there during the second flush.

Here's an example of a rogue spawn jar about 2 days after shaking at full colonization.

At a glance you'd think it's recovering fine. No slime or smell or anything like that usually associated with bacteria.

And if you happen to notice something like this in your tubs it's pretty clear the grain had not recovered in the spot where the Trich started growing.


Edited by Kizzle (06/03/15 05:36 PM)


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: Kizzle]
    #21758363 - 06/03/15 05:44 PM (8 years, 7 months ago)

Well this is a fountain of information.  Just wanted to throw my 2 cents in. I think everyone has a run in with spawn busts. I currently have a massive out break that I lost all of my jars to. I made up some more petri dishes to start over and those contaminated before I ever transferred mycelium to them. The funk in my place got so bad I realized 1 thing.

The number 1 cause of all of my contamination issues recently was, LAZYNESS. I got just plain lazy when I poured my petris, and let them in the glove box over night to cool before wrapping them with syran. I never checked my LC's on agar to make sure they were clean, and I didn't transfer my original agar enough to clean out the hiding trich in them. It's all a process, and I have learned that I just get to damn lazy for this hobby. My head is all wrapped up in my aquaponics system anyway.

But yeah good looking mold blocker, does it work on bacteria?


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 25 minutes
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: Sockadin]
    #21758478 - 06/03/15 06:08 PM (8 years, 7 months ago)

One other thing. Let the condensation disappear completely after pouring before using agar plates if you can. Bacteria will sometime appear on a plate as very slight discoloration of the surface. In the few days it takes for the condensation to completely disappear so can actually get a good look there's enough time for the bacteria to completely cover surface and when there's no edge of a bacteria colony to be seen it's less noticeable. I know in at least one case that's how it managed to slip by :tongue:


--------------------


Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,064
Trusted Cultivator
Re: How I overcome heavy room contamination! [Re: Mad Season]
    #21758752 - 06/03/15 07:17 PM (8 years, 7 months ago)

Quote:

Mad Season said:
Can I point out that I don't really dunk anymore because I notice trich from it. Just mist it heavily between flushes and keep it shimmering wet. I get quite a few flushes doing it and it remains pretty contam resistant :smile:




:whathesaid:

Everyone gets 5 - 8 flushes when they first start growing. Especially if they are in a newer building or a well kept older one.  After your first big contamination flushes go down to two.

I get two solid flushes before I see green. I used to gasp and wonder at the people who said that they throw tubs away as soon as the second flush is over but now I see that this really has to be the standard practice.

Third flushes are hardly ever yielding the kind of weight worth waiting around for in my case.  So now I toss them too.  The one thing I can say holds true for me about third and later flushes is that the shrooms always seem to be significantly bigger later on.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | Next >  [ show all ]

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* overcoming bacterial contamination - safe? Chibboleth 2,748 13 01/24/04 12:32 PM
by The_Mushroomm_Man
* Hit hard by trich... =( Onetwothree 2,871 19 07/07/05 04:22 PM
by sludge
* contamination uh oh! Asmbandits 608 3 04/06/03 05:21 PM
by SixCee
* The use of salt in fighting trichoderma mold
( 1 2 3 4 5 all )
Hippie3 66,177 86 03/01/23 08:28 PM
by 6The6Despised6One
* Contamination Identification & Subjugation NutsackRucksack 13,844 4 11/19/20 09:38 PM
by Goat 2020
* Remedy for Trich? (for you pro's)
( 1 2 all )
upupup 27,549 27 06/07/23 02:11 AM
by johnukguy
* Green Mold - Trichoderma harzianum example (pics) MAIA 13,732 8 07/16/21 09:49 AM
by sandman420
* interesting notes on trich. chineseguy 2,733 1 07/01/03 03:52 PM
by MAIA

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
5,646 topic views. 51 members, 260 guests and 40 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.01 seconds on 15 queries.