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Invisibleslapphappypill
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How I overcome heavy room contamination!
    #21736430 - 05/29/15 11:25 AM (8 years, 7 months ago)

To preface, it's been a while since I've posted. However, I went through a real bad trich contamination that left me running spawn with 100% success and 0-5% success after spawning to tubs with damion 50/50 bucket tech.

So, if you have an issue where your fruiting area has contaminated everything, and no matter how well you clean your tubs/buckets, you still get the mean green guarenteed, check this out! http://www.zoro.com/mold-armor-moldmildew-stain-remover-32-oz-fg502/i/G3535357/

coat the inside and outside of your tubs/buckets and let sit overnight. Then wash off the cleaner well, let air dry. Then use some clorox wipes to clean any extra debris before you spawn to bulk. I still use plenty of disinfectant to keep things clear as well throughout the process.

All said and done, I was back on track with 100% success and zero contamination!!

Hope this helps anyone struggling with the same issue I had :-)


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We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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OfflineMonkeyJesusFresco
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
    #21736482 - 05/29/15 11:40 AM (8 years, 7 months ago)

I'm just not very convinced that "room contamination" is a real concern;
sounds like you had contaminated spawn, but whatever :shrug:

I got clean spawn and bulks, and there's like...trich all over my fucking place, house plants in my 'lab', fuck'n dirty carpet everywhere...all my furniture, including the table I use for my still-air-box was in storage, next to a swamp, and came with its own mold contamination (don't know what it was, but it was green and white, and in such a quantity I almost gave up the hobby on the spot)...I just...ya know? I'm not sold on "room contamination" being a thing :rolleyes:

but I guess it could be... I know sterile technique is a thing, that might account for my lack of contamination...I dunno :confused:


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OfflineAero
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Re: How I overcome heavy room contamination! [Re: MonkeyJesusFresco]
    #21736575 - 05/29/15 12:03 PM (8 years, 7 months ago)

Quote:

MonkeyJesusFresco said:
I'm just not very convinced that "room contamination" is a real concern;
sounds like you had contaminated spawn, but whatever :shrug:

I got clean spawn and bulks, and there's like...trich all over my fucking place, house plants in my 'lab', fuck'n dirty carpet everywhere...all my furniture, including the table I use for my still-air-box was in storage, next to a swamp, and came with its own mold contamination (don't know what it was, but it was green and white, and in such a quantity I almost gave up the hobby on the spot)...I just...ya know? I'm not sold on "room contamination" being a thing :rolleyes:

but I guess it could be... I know sterile technique is a thing, that might account for my lack of contamination...I dunno :confused:



:whathesaid:
i live in an old house man, no problem with contams :wink: check your technique, are u properly pasteurizing ur substrate?


--------------------
SPREAD THE SPORES



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InvisiblePirateSwazey
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Re: How I overcome heavy room contamination! [Re: Aero]
    #21736590 - 05/29/15 12:08 PM (8 years, 7 months ago)



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BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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InvisibleMad Season
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Re: How I overcome heavy room contamination! [Re: PirateSwazey]
    #21737952 - 05/29/15 06:23 PM (8 years, 7 months ago)

I live in an old 70s farm house with dogs lol. It's also small so I do all my SAB work in the kitchen or living room. They say a walk outside gets you covered in more trich than a contamination inside. Working with clean cultures and clean spawn is what gave me my best success rates


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Offlinemushpunx
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Re: How I overcome heavy room contamination! [Re: Mad Season]
    #21738225 - 05/29/15 07:22 PM (8 years, 7 months ago)

I know Ive read RR say he used to run his flowhood in his bedroom which is carpeted and his dog sleeps in there :lol:

I have noticed a higher rate of contamination in subsequent grows after something happens (like I didnt notice a tub in the stack has trich). Right now I am battling some other sort of green mold that seems to start off green instead of white..
I had a tub with it in my grow room and I still see it every couple of grows


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Amateur Mycologists United
AMU Q&A


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Invisibleelasticaltiger
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Re: How I overcome heavy room contamination! [Re: mushpunx]
    #21739109 - 05/29/15 10:53 PM (8 years, 7 months ago)

Shmuvbox:

http://www.shroomery.org/forums/showflat.php/Number/11353633

Proper Bucket Pasteurization:
http://www.shroomery.org/forums/showflat.php/Number/19907089/fpart/1/vc/1

I've had my own bouts with contamination. I think anyone who does this for more than a few months eventuallys meets the mean green.  I just wipe tubs down with alcohol between runs.  Practice good technique and I've gifted with quick, smooth hands.


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First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

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No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

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Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Invisibleelasticaltiger
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Re: How I overcome heavy room contamination! [Re: elasticaltiger]
    #21739114 - 05/29/15 10:54 PM (8 years, 7 months ago)

I am curious about that chemical though. If it improves success then why not?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineMonkeyJesusFresco
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Re: How I overcome heavy room contamination! [Re: elasticaltiger]
    #21739159 - 05/29/15 11:03 PM (8 years, 7 months ago)



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Invisibleelasticaltiger
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Re: How I overcome heavy room contamination! [Re: MonkeyJesusFresco]
    #21742201 - 05/30/15 06:48 PM (8 years, 7 months ago)

Quote:

MonkeyJesusFresco said:





OMG I love xkcd. This one is my favorite to share here for when n00bs ask about using bleach and alcohol.



--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Invisibleslapphappypill
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Re: How I overcome heavy room contamination! [Re: elasticaltiger]
    #21752207 - 06/02/15 11:01 AM (8 years, 7 months ago)

Just to followup, my spawn was never contaminated...

I had 100% success with spawn runs in jars and bags... Please don't take my years not on the forums for me not knowing my process. I work with a 2X4 flowhood (which had a tear in it a few months ago, fixed with silicon). But I could tell my 95-100% contams were coming in after I spawned to bulk, right before fruiting it during fruiting. I've even built a secret area for my tubs ;-)

So long story short, i'm nothing close to being new to this, but thanks for the concern. My contams were due to getting trich badly before I figured out about the tear in my flowhood. The tubs all stayed contaminated, even after regular cleaning with bleach etc.

So the reason for my post, was to inform those who get a real bad trich contamination in their grow area to try the mold killer above! It works fantastic, and since then, i've not changed my practice and see 100% success :jah:

-SHP




--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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OfflineKizzle
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
    #21752825 - 06/02/15 01:30 PM (8 years, 7 months ago)

Quote:

slapphappypill said:
Just to followup, my spawn was never contaminated...

I had 100% success with spawn runs in jars and bags... Please don't take my years not on the forums for me not knowing my process. I work with a 2X4 flowhood (which had a tear in it a few months ago, fixed with silicon). But I could tell my 95-100% contams were coming in after I spawned to bulk, right before fruiting it during fruiting. I've even built a secret area for my tubs ;-)

So long story short, i'm nothing close to being new to this, but thanks for the concern. My contams were due to getting trich badly before I figured out about the tear in my flowhood. The tubs all stayed contaminated, even after regular cleaning with bleach etc.

So the reason for my post, was to inform those who get a real bad trich contamination in their grow area to try the mold killer above! It works fantastic, and since then, i've not changed my practice and see 100% success :jah:

-SHP






Of course Trichoderma has to be present for it to contaminate something. However I've noticed bacteria in the spawn has a major effect on whether the Trichoderma once present is able to grow on the substrate or not. So contaminated spawn doesn't just mean the Trichoderma itself being in the spawn even though that's what you were running into.


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Offlinemushpunx
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Re: How I overcome heavy room contamination! [Re: Kizzle]
    #21754146 - 06/02/15 06:28 PM (8 years, 7 months ago)

Kizzle - what sort of advice would you give to those of us who are still struggling with losing tubs to trich?

I mean I do the best I can, I lysol my hood then run it for a bit, shower/clean up fresh scrubs tyvek,gloves, mask... blow torch my tools wash anything that goes in front of my hood down with ISO, I try to use proper hand motions :shrug:

Despite my best efforts at proper sterile tek and culture work I still end up losing many tubs to trich.
Is there anything else I should be doing, maybe environment wise?

I have to spawn way more tubs than I need to to ensure nice harvests. 

Just open to sugguestions! :lol:


--------------------

Amateur Mycologists United
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Offlinemushpunx
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Re: How I overcome heavy room contamination! [Re: slapphappypill]
    #21754187 - 06/02/15 06:37 PM (8 years, 7 months ago)

"But I could tell my 95-100% contams were coming in after I spawned to bulk, right before fruiting it during fruiting"


This right here sounds like its your spawn man. You wont see trich in your spawn unless you are lucky and it goes green in the jar.
Quite often my masters will look/smell/behave fine otherwise, same with reciving jars from G2G, my tubs will colonize just fine.

The trich will show up once you put your tubs into fruiting conditions, you are in effect fruiting the trich as well. This is why it is so difficult to detect in your spawn.

Good rule of thumb is if your tubs are going green before or during first flush, then it is from contaminated spawn.... really you cant say for sure your spawn is clean untill you fruit your sub.


--------------------

Amateur Mycologists United
AMU Q&A


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InvisibleGhatti
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Re: How I overcome heavy room contamination! [Re: mushpunx]
    #21754190 - 06/02/15 06:38 PM (8 years, 7 months ago)

Yes oh mighty kizzle please do share. I'm hoping using sleeves in my bags have solved my problem but if not I need your wisdom as well.

Mush, are you following the rule of "first air"? Sure your hood is built right and laminar?


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Offlinemushpunx
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Re: How I overcome heavy room contamination! [Re: Ghatti]
    #21754251 - 06/02/15 06:48 PM (8 years, 7 months ago)

Quote:

Ghatti said:
Yes oh mighty kizzle please do share. I'm hoping using sleeves in my bags have solved my problem but if not I need your wisdom as well.

Mush, are you following the rule of "first air"? Sure your hood is built right and laminar?







Yea man built her myself :wink:
I test the flow every once in a while and I test it by leaving blank plates open for a while. It seems solid


--------------------

Amateur Mycologists United
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InvisibleGhatti
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Re: How I overcome heavy room contamination! [Re: mushpunx]
    #21754273 - 06/02/15 06:52 PM (8 years, 7 months ago)

Dunno then. Is your agar work clean?


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OfflinecronicrFacebook
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Re: How I overcome heavy room contamination! [Re: Ghatti]
    #21754718 - 06/02/15 08:31 PM (8 years, 7 months ago)

holy lots of my old favorite posters here, nice to see ya slap/monkey/pirate/tiger/kizzle/areoghattti lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleGhatti
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Re: How I overcome heavy room contamination! [Re: cronicr]
    #21754751 - 06/02/15 08:40 PM (8 years, 7 months ago)

Is bl still around? Or blojo?


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OfflineMonkeyJesusFresco
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Re: How I overcome heavy room contamination! [Re: Ghatti]
    #21754781 - 06/02/15 08:47 PM (8 years, 7 months ago)

:hi: cron!


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