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OfflineMushSicle
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Registered: 04/14/15
Posts: 58
Last seen: 8 years, 6 months
f u contams
    #21735863 - 05/29/15 07:31 AM (8 years, 7 months ago)

After shaking 8 quart jars and 4 didn't recover and colonize, after 5 days of waiting and scrutinizing I took them out in my garage and cracked them individually and they smelled sour, yuck and toss. Ran through dish washer and started another 4. Luckily this time rather than ms I've been working with agar and have isolates. Round 2 slut contams.


Edited by MushSicle (05/29/15 07:32 AM)


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Offlinehamloaf
Loaf of Fam.
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Registered: 12/23/09
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Loc: Oklahoma. Flag
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Re: f u contams [Re: MushSicle]
    #21735912 - 05/29/15 07:58 AM (8 years, 7 months ago)

:deathstar:

Sounds like your contam issue came from improper sterilization of media, and/or overly hydrated grain content, and/or dirty inoculm, and/or colonizing/incubating at too high a temperature range.


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OfflineJenn
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Registered: 04/27/15
Posts: 200
Last seen: 7 years, 11 months
Re: f u contams [Re: hamloaf]
    #21736309 - 05/29/15 10:45 AM (8 years, 7 months ago)

You should give more detail about the event leading up to the contamination.  What substrate, how did you sterilize it and in what, method of inculation, sterile technique, where the jars were store after inoculation and at what temperature etc...  With more information, what may not be immediately apparent to you may be obvious to someone reading your post.  Don't give up cause you can do it!  Good luck!


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Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.


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OfflineMushSicle
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Last seen: 8 years, 6 months
Re: f u contams [Re: Jenn]
    #21736889 - 05/29/15 01:33 PM (8 years, 7 months ago)

Rye grass seed I sterilized in a PC and nocced in a still air box, I think my substrate was too wet. It was noticeably very moist in the containers, overly moist. So I put the grains out on newspaper under a fan, then loaded 4 quart jars today and pc'd at 15-16 psi for 60 mins. Letting them cool gradually in pc as we speak


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OfflineJenn
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Registered: 04/27/15
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Last seen: 7 years, 11 months
Re: f u contams [Re: MushSicle]
    #21736928 - 05/29/15 01:50 PM (8 years, 7 months ago)

So this is a new attempt?  What are you growing on the seed?  And how long did you PC the see and at what PSI?


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Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: f u contams [Re: Jenn]
    #21739890 - 05/30/15 06:16 AM (8 years, 7 months ago)

Just to let you know ham is most likely right. Grains have endospores that seem to be barely able to survive 2 hours in the pc at 15 psi. 1 hour isn't enough.. 90 minutes isn't enough in my opinion.

Moisture content is also critical.


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Invisibleblindingleaf
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Registered: 07/19/13
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Re: f u contams [Re: Mad Season]
    #21739904 - 05/30/15 06:27 AM (8 years, 7 months ago)

Quote:

Grains have endospores that seem to be barely able to survive 2 hours in the pc at 15 psi. 1 hour isn't enough.. 90 minutes isn't enough in my opinion.




they sit 290 degrees underneath palm trees, smoking beadies as they burn their calories :blazed:


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InvisibleMad Season
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Re: f u contams [Re: blindingleaf]
    #21739908 - 05/30/15 06:31 AM (8 years, 7 months ago)

Hahaha those damn lazy bacterial endospores :stoned:

Freeloading our taxes :drunkdriver:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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