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Offlinejhud6403
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brf tek produce weak cubes or ways to improve?
    #21735500 - 05/29/15 03:19 AM (8 years, 7 months ago)

As my research continues I keep running into claims that brf tek produce weak cubes. If true what are some ways to improve on recipe or is cube just a cube?


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OfflineZuto18
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Re: brf tek produce weak cubes or ways to improve? [Re: jhud6403]
    #21735515 - 05/29/15 03:29 AM (8 years, 7 months ago)

Cubes a cube man brf produces fewer shrooms than bulk but its funny you say this i just started a little experiment in which i use Dark Rye Flour as apposed to Brown Rice Flour too see if i can get a bigger yeild/higher potency as apposed to faster colonization time you can follow that here theres also a link to another thread within the one below that you might wanna give a read
http://www.shroomery.org/forums/showflat.php/Number/21735344


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Ever make mistakes in life? Lets make them birds, yeah, they are birds now. -Bob Ross
I sense a fungus among us... -me when I'm trippin'


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OfflineMajickMuffin
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Re: brf tek produce weak cubes or ways to improve? [Re: Zuto18]
    #21735553 - 05/29/15 04:00 AM (8 years, 7 months ago)

I wouldn't say BRF makes weak cubes. I've had extremely potent, and average potent shrooms grow from BRF. When using multispore syringes I'd say it's all based on chance.


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Invisiblewildernessjunkie
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Re: brf tek produce weak cubes or ways to improve? [Re: MajickMuffin]
    #21735652 - 05/29/15 05:31 AM (8 years, 7 months ago)

BRF is plenty nutritious and produces potent enough cubes. If you want something more potent then you will need to start playing with isolates or clones. Either that or switch to Pans.


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Offlinejhud6403
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Re: brf tek produce weak cubes or ways to improve? [Re: wildernessjunkie]
    #21735801 - 05/29/15 07:00 AM (8 years, 7 months ago)

Thank you all for your response. Zuto 18  I like the reading material and I'm looking in to using millet with brf. I'll keep reading your post to see how it goes.

Yes I had a feeling it was genetics
But being this is my first time I wanted some opinions on the matter. You wouldn't believe how many post there are of people arguing about that. I might get into isolates but at a much later date when I have more experience. I'll stick with the tek and my test modification for now and see how things turn out.


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OfflineZuto18
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Re: brf tek produce weak cubes or ways to improve? [Re: jhud6403]
    #21736130 - 05/29/15 09:38 AM (8 years, 7 months ago)

Sweet id be interested to see how that goes with using millet and brf who knows you might make a discovery let me know when you decide to do that and i will so watch =D


--------------------

Ever make mistakes in life? Lets make them birds, yeah, they are birds now. -Bob Ross
I sense a fungus among us... -me when I'm trippin'


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Offlinejhud6403
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Re: brf tek produce weak cubes or ways to improve? [Re: Zuto18]
    #21738016 - 05/29/15 06:41 PM (8 years, 7 months ago)

cool my first five will be a half and half of both BRF and Millet. then next five will be strait BRF.


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Offlinemycomaniac1402
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Re: brf tek produce weak cubes or ways to improve? [Re: jhud6403]
    #21738125 - 05/29/15 07:06 PM (8 years, 7 months ago)

Definitely not the substrate IMO. Some people say mushrooms grown on coir are weak compared to manure and thats definitely not true. Its all about the shroom or substrain thats prominent. Interesting how each individual can be vastly different. Some of the most potent ive had were from coir, and brf cakes both.

I Personally haven't ever had issues with multispore , maybe its just my luck. Good vibes on your next run


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Invisibledjmako7
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Re: brf tek produce weak cubes or ways to improve? [Re: mycomaniac1402]
    #21738154 - 05/29/15 07:10 PM (8 years, 7 months ago)

Potency comes from two things.

Genetics and environmental conditions. Substrate has very little to do with potency unless its contaminated.


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OfflineZuto18
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Re: brf tek produce weak cubes or ways to improve? [Re: djmako7]
    #21738449 - 05/29/15 08:18 PM (8 years, 7 months ago)

I disagree tryptophan does increase cube potencyhttp://www.shroomery.org/forums/showflat.php/Number/13200397/fpart/all/vc/1
And DRF has 66% tryptophan brf has 33% so you do the math...


--------------------

Ever make mistakes in life? Lets make them birds, yeah, they are birds now. -Bob Ross
I sense a fungus among us... -me when I'm trippin'


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InvisiblePastywhyteMDiscord
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Re: brf tek produce weak cubes or ways to improve? [Re: Zuto18]
    #21738789 - 05/29/15 09:45 PM (8 years, 7 months ago)

That discourse in that link does not demonstrate that an increase in tryptophan increases potency, but it does show that it is the source for the bioconversion. Thats never been in question. The real issue is will simply providing more cause an increase?  I suspect no. Simply put an increase in availability by no means indicates that a culture can make use of it. I'm no biologist but I think of it like this. You have two brf cakes each inoculated with a different isolate. Isolate A is a very potent strain and isolate B is an average potency strain. They both come out strong and average respectively. Yet isolate B had access to as much tryptophan and by extension as much tryptomine as culture A. Yet potency was weaker. Why? Because it was unable to make as effective use of it. Therefore adding more will only make a difference insomuch as the culture is able to utilize it.

This I suspect is the driving force behind potency differences with other species as well. Speaking of other species there has been some (older) research that suggests the ability of certain fungi to convert tryptophan into alkaloids is dependent on zinc ions in the substrate.  I believe this was demonstrated with aspergillus. So assuming that alkaloids in higher fungi are produced in a similar manner the simple addition of more is not going to make it immediatly bio available.

Meh, I will admit most of this organic chemistry is way over my head but I have grown enough cubes to know that I can get brain melting results from the same exact sub that only did so so with a different culture.


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InvisiblebodhisattaMDiscordReddit
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Re: brf tek produce weak cubes or ways to improve? [Re: Pastywhyte]
    #21739065 - 05/29/15 10:42 PM (8 years, 7 months ago)

That and the old one about the most potent species growing naturally on low nutrients


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Offlinejhud6403
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Re: brf tek produce weak cubes or ways to improve? [Re: bodhisatta]
    #21740594 - 05/30/15 10:52 AM (8 years, 7 months ago)

Very interesting. So I started my first batch of 4 with millet and then BRF. I'm expecting hopefully higher yields over potency. I inculcated with golden teachers from TSD. I have a little over 5 cc of that left and two more syringes of B+ and south American. hopefully my sterile methods were adequate.


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