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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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LC lid
#21734534 - 05/28/15 09:50 PM (8 years, 7 months ago) |
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If you make an lc lid with one of those syringe filters in the cap, can you invert it?
Say I made an lc lid with one of those injection ports and a syringe filter, can I simply invert it and draw what's needed through the port?
I see those lids everywhere but I've always thought they were just for injecting spores or what have you INTO the jar.
I remember years ago reading (I think it was TL, and if you haven't been around long enough to know who TL is please move along) about a no tilt lc lid. Basically had a straw/tube in the jar.
Do I just need to tilt my chosen jar to get the solution to the port without wetting the filter?
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rxb
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Re: LC lid [Re: Ghatti]
#21734581 - 05/28/15 10:01 PM (8 years, 7 months ago) |
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the one from outgrow invert fine, the SHIP will leak a bit if its been used (i dap a bit of high temp silicone on after i use them to keep the drips down)
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
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Re: LC lid [Re: rxb]
#21734598 - 05/28/15 10:04 PM (8 years, 7 months ago) |
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Didn't know if you had to tilt just so or could just invert, good stuff.
Now off to find a gallon jar...........
May have to just settle for a large pickle jar eh?
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rxb
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Re: LC lid [Re: Ghatti]
#21734607 - 05/28/15 10:05 PM (8 years, 7 months ago) |
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i haven't found a gallon jar with a standard wide mouth yet. quarts do almost everything i need anyhow.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
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Registered: 06/23/14
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Re: LC lid [Re: rxb]
#21734648 - 05/28/15 10:17 PM (8 years, 7 months ago) |
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Likely a quart jar would be fine for me as well but I'd rather have a gallon laying around instead of making 4 LCS in the same time and having to test each one out.
If I make a bigger one and verify it clean then all I have to worry about is not sticking a dirty needle in it.
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rxb
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Re: LC lid [Re: Ghatti]
#21734691 - 05/28/15 10:30 PM (8 years, 7 months ago) |
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i think a quart of LC or LI is plenty it only takes a few drops.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
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Re: LC lid [Re: rxb]
#21734698 - 05/28/15 10:33 PM (8 years, 7 months ago) |
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That defeats a main purpose of lc/li for me. If I use either I use enough for the bag to basically explode in growth all over and be damn near done in 5 days after recovering.
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rxb
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Re: LC lid [Re: Ghatti]
#21734882 - 05/28/15 11:10 PM (8 years, 7 months ago) |
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only takes a few days with a few drops lol
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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hamloaf
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Re: LC lid [Re: Ghatti]
#21735639 - 05/29/15 05:20 AM (8 years, 7 months ago) |
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Don't listen to poor advice above. You are correct in your logic of the more LC you inoculate your substrate with the faster colonization will take place.
Fungi perfecti has gallon jars for sale with the high-quality white, plastic, PP5 media closures. They come in a pack of 4. http://www.fungi.com/product-detail/product/1-gallon-glass-jars-with-lids-set-of-4.html
When opting to do LC in large containers, such as a gallon jars, you are going to need to increase measures of gas exchange, as well as, sterilize for a lot longer than 45 minutes. Solution for gas exchange, and sterilization measures for gallon sized jars of LC : use 2 whatman filters, and sterilize at 15-20 psi for at least two hours.
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rxb
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i cant believe i missed those jars.
colonization happens fast, with or without a shit ton of lc.
assuming isolation of course more is faster but... blah.
pointless imo.
it only takes a few drops.
but gallon jars are the tits.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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rxb
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Re: LC lid [Re: rxb]
#21735907 - 05/29/15 07:57 AM (8 years, 7 months ago) |
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i dont believe those jars are wide mouth (76mm) since it says they take a 110mm sfd.
so thats back to the drawing board.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
Edited by rxb (05/29/15 07:59 AM)
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hamloaf
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Re: LC lid [Re: rxb]
#21735917 - 05/29/15 08:01 AM (8 years, 7 months ago) |
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The lids on those gallon jars are 125mm. Wide mouth quart jars, and half pint wide mouth media closures are 90mm. HUGE difference.
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rxb
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yeah i think thats what he is after. gallon jars which he can buy lids from out-grow for.
maybe i misunderstood him.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
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Registered: 09/15/12
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Quote:
hamloaf said: You are correct in your logic of the more LC you inoculate your substrate with the faster colonization will take place.
I must agree with the above. When I was first starting with LC I would only use a half cc or so as it was what was being touted at the time. But even with a vigorous shake it would never cover as much ground as I wanted initially and recovery was often weak. If you only made a pint of LC a half cc would be enough to inoculate 946 quarts. Personally that would be more than I could need and I would simply just end up pitching most of it (I don't like to keep LC around, even in the fridge).
So I am generous with it. I see better distribution and faster recovery.
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rxb
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i feel like i must either be ok with a few days... or that im not having the same experience as you guys.
shrug
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
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Registered: 06/23/14
Posts: 1,733
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Re: LC lid [Re: rxb]
#21736048 - 05/29/15 08:55 AM (8 years, 7 months ago) |
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Are you using a thick lc?
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Pastywhyte
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Re: LC lid [Re: rxb]
#21736075 - 05/29/15 09:10 AM (8 years, 7 months ago) |
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I have yet to see anyone post a grow log where a few drops or even a cc could be shaken throughout a jar and have full colonization a day or two past recovery. I know for myself I can't get it to work like that. But if I used 5cc and shake well recovery happens in two days and two days later its done. If you can't see full colonization in 5-7 days with LC its not worth the risk of using it IMO.
If you're able to do better than that, a grow log detailing the process would be of great value.
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rxb
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from spores to full is 9 days. from lc to full is 4 to 6 ..
lc = shake every 2 days
spores = shake every 3.
its 3 shakes to full colonization
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
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Posts: 1,733
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Re: LC lid [Re: rxb] 1
#21736155 - 05/29/15 09:52 AM (8 years, 7 months ago) |
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I call shenanigans. Can't go from spores to full in 9 days
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rxb
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Re: LC lid [Re: Ghatti]
#21736161 - 05/29/15 09:54 AM (8 years, 7 months ago) |
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uhm... yes you can
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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rxb
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Re: LC lid [Re: rxb]
#21736170 - 05/29/15 09:59 AM (8 years, 7 months ago) |
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the only variant i really see is the time it takes for the myc to be about quarter sized... thats the first period... ive seen it take a week it usually takes `3 days
then its shake like hell... wait three days shake like hell wait three days... etc ... with spores.
with lc... in 2 days you'll have a quarter sized bit....
and in 2 days you can shake it... 2 days shake 2 days shake... and the next day its usually filling the jar.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
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Posts: 1,733
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Re: LC lid [Re: rxb]
#21736210 - 05/29/15 10:16 AM (8 years, 7 months ago) |
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You may be able to colonize a single kernel. Spores are going to take 4-9 days to even show growth.
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rxb
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Re: LC lid [Re: Ghatti]
#21736217 - 05/29/15 10:18 AM (8 years, 7 months ago) |
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well thats not what im seeing.
i have no other frame of reference.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
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Registered: 09/15/12
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Re: LC lid [Re: rxb] 1
#21736220 - 05/29/15 10:18 AM (8 years, 7 months ago) |
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That's too long. With a decent amount of inoculate in 2 days the entire jar is showing recovery. 2-3 days after that its done. The number of shakes you're doing is going to really deplete energy making for a longer spawn run and possibly lower BE in the end. I actually doubt that you're even seeing that quick of recovery with that much shaking.
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rxb
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well i dont know what to tell you 
its what i see... over and over. i might do pictures sometime, im really bad at taking time out to do photos for stuff, which is odd because i have pro level photo equipment.
but if i wasnt so horrible at photodocumenting stuff i'd just tell you i'd put my photos where my mouth is.
shrug.
i guess i dont know what other people see.
right now im working on, making agar jars, colonizing those adding sterile water to make a lc/li sort of deal... and using that.
because its easy...and if you isolate a strain once, you can do it more or less endlessly. and pause it for a long while by simply refrigerating.
and i havent even photographed that.
rebooting the system soon to start using "hamloaf" agar
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
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Re: LC lid [Re: rxb]
#21736487 - 05/29/15 11:42 AM (8 years, 7 months ago) |
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Well I am familiar with agar, isolation and the keeping of cultures long term. But that is besides the point. I actually think that your claim of 8-10 days for full colonization is possibly a little optimistic but given a strong culture not impossible. However that is too long of a timeframe given how much inoculate can be derived from LC expansion. There is simply no need to use only a few drops.
If you needed to inoculate 2000 quarts then to suggest rationing every last drop of LC in a quart is a little odd given how simple it can be to create that volume of culture. The difference between making one quart of LC vs making 10 is moot, you could do it with a single plate. At that point you have halved your colonization time thus lengthening your spawning and fruiting window and without requiring the colony to constantly rebuild itself. This will pay off with a larger yield and faster spawn runs. Anyone in need of that much spawn will surely appreciate the tradeoff.
Of course if you don't need 2000 quarts of spawn then the question is why are you rationing inoculate in such a manner? LC is not a good way to store culture, even refridgerated its still growing and producing waste. Better to keep slants or at least plates for any period of storage over a month IMO.
If what your doing is good enough for you I won't say anymore. But I do encourage you to try it for yourself. You might be pleasantly surprised.
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rxb
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nah the storage state is a colonized agar jar.
i haven't tested how long it goes, but its kind like a huge slant that just takes up WAY more space. but who cares, i don't have 100s of isolates.
the jar vs plate thing, is that once you get it to an isolate, you never really need to have lab equipment in my cycle.
so in theory, you could work in a bag or such for your isolation tek, and then, once you have a clean isolated strain you could carry on indefinitely, with a pc and a dream.
im just playing. as for the timeline shrug. its working for me for now. if you think its taking longer than it really is, im not sure how it hurts either of us.
anyhow, if my timeline is faster than what others see i guess im sorry for giving false hope, or whatever crime i am guilty of 
but that's what seems to be normal for me.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
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Re: LC lid [Re: rxb]
#21736554 - 05/29/15 11:59 AM (8 years, 7 months ago) |
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Go back and re read what I wrote. I'm not saying your timelime is wrong. I'm saying you could be getting it done faster that you currently are, with less wasted energy resulting in a faster spawn run and better yield. Meh.
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rxb
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ah..
well im happy... i dont need it faster than this, im not a dealer
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
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Re: LC lid [Re: rxb]
#21736626 - 05/29/15 12:20 PM (8 years, 7 months ago) |
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Fair enough. Neither am I. But I would like to note that the main reason to even use LC or any liquid form of inoculate to grow many species typically started on grain, is that when we use grain we are working within a window of time. The whole point of liquid is to lengthen that window by speeding the time colonization takes. Its the chief reason to risk using it in the first place. Less destruction of the colony and faster colonization can mean less risk from endospores. It also contributes to a more aggressive state which again lowers the risk of contams during spawning. Also repeated or excessive shaking is counterproductive and expansion should take place in such a manner that it is limited as much as possible.
No matter what a persons reason for growing any species, faster results and higher yield should never be seen as bad. You say you don't need much and you store culture on agar. So then I can only presume your simply dumping the excess LC at the end of a grow. Waste not man. You literally have zero to lose but a lot of upside you're missing out on.
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rxb
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right now i do dump a lot because im playing with variables.
i also just knocked up jars that are 2 months old to see what i get.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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rxb
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Re: LC lid [Re: rxb]
#21736652 - 05/29/15 12:28 PM (8 years, 7 months ago) |
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this has so little to do with fruits for me right this minute 
i mean it has A BIT To do with fruits.. but you know... its the puzzle.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Re: LC lid [Re: rxb]
#21736706 - 05/29/15 12:39 PM (8 years, 7 months ago) |
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Fair enough. But if you recommend something to someone here on these boards its good to keep in mind that for them its usually about the fruits. Most people want a good yield and success rate. If they're simply looking for a proof of concept or observational data they either state it in the OP or don't bother with a question at all.
Anyway I think this small aside is wrapped up. Good discourse
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blackout


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Re: LC lid [Re: Ghatti]
#21739820 - 05/30/15 04:49 AM (8 years, 7 months ago) |
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Quote:
Ghatti said: If you make an lc lid with one of those syringe filters in the cap, can you invert it?
I often used LCs with no filter at all. I would not fill the jars more than 1/3rd full. This allows you to shake the hell out of the jars to break up myc and aerate the liquid. If the jar is too full you will have a hard time pulling much liquid out due to the vacuum forming. I would let my myc settle and only pull out really dense myc water, so I would never use all the liquid in the jar anyway. If you did want more the jar could be cracked open in an SAB to let new air in. I also had some lids with 2 holes to allow a filtered syringe to be injected to let new air in, this means you do not need filters for all the jars.
these jars had 30ml of LC added to them each, plenty of growth 3 days later and no shaking needed besides the initial shake when the LC was added

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