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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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LC lid
#21734534 - 05/28/15 09:50 PM (8 years, 7 months ago) |
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If you make an lc lid with one of those syringe filters in the cap, can you invert it?
Say I made an lc lid with one of those injection ports and a syringe filter, can I simply invert it and draw what's needed through the port?
I see those lids everywhere but I've always thought they were just for injecting spores or what have you INTO the jar.
I remember years ago reading (I think it was TL, and if you haven't been around long enough to know who TL is please move along) about a no tilt lc lid. Basically had a straw/tube in the jar.
Do I just need to tilt my chosen jar to get the solution to the port without wetting the filter?
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
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Re: LC lid [Re: Ghatti]
#21734581 - 05/28/15 10:01 PM (8 years, 7 months ago) |
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the one from outgrow invert fine, the SHIP will leak a bit if its been used (i dap a bit of high temp silicone on after i use them to keep the drips down)
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: LC lid [Re: rxb]
#21734598 - 05/28/15 10:04 PM (8 years, 7 months ago) |
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Didn't know if you had to tilt just so or could just invert, good stuff.
Now off to find a gallon jar...........
May have to just settle for a large pickle jar eh?
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
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Re: LC lid [Re: Ghatti]
#21734607 - 05/28/15 10:05 PM (8 years, 7 months ago) |
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i haven't found a gallon jar with a standard wide mouth yet. quarts do almost everything i need anyhow.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: LC lid [Re: rxb]
#21734648 - 05/28/15 10:17 PM (8 years, 7 months ago) |
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Likely a quart jar would be fine for me as well but I'd rather have a gallon laying around instead of making 4 LCS in the same time and having to test each one out.
If I make a bigger one and verify it clean then all I have to worry about is not sticking a dirty needle in it.
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rxb
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Re: LC lid [Re: Ghatti]
#21734691 - 05/28/15 10:30 PM (8 years, 7 months ago) |
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i think a quart of LC or LI is plenty it only takes a few drops.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: LC lid [Re: rxb]
#21734698 - 05/28/15 10:33 PM (8 years, 7 months ago) |
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That defeats a main purpose of lc/li for me. If I use either I use enough for the bag to basically explode in growth all over and be damn near done in 5 days after recovering.
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rxb
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Re: LC lid [Re: Ghatti]
#21734882 - 05/28/15 11:10 PM (8 years, 7 months ago) |
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only takes a few days with a few drops lol
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
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Re: LC lid [Re: Ghatti]
#21735639 - 05/29/15 05:20 AM (8 years, 7 months ago) |
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Don't listen to poor advice above. You are correct in your logic of the more LC you inoculate your substrate with the faster colonization will take place.
Fungi perfecti has gallon jars for sale with the high-quality white, plastic, PP5 media closures. They come in a pack of 4. http://www.fungi.com/product-detail/product/1-gallon-glass-jars-with-lids-set-of-4.html
When opting to do LC in large containers, such as a gallon jars, you are going to need to increase measures of gas exchange, as well as, sterilize for a lot longer than 45 minutes. Solution for gas exchange, and sterilization measures for gallon sized jars of LC : use 2 whatman filters, and sterilize at 15-20 psi for at least two hours.
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rxb
n00b-sabot



Registered: 08/24/13
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i cant believe i missed those jars.
colonization happens fast, with or without a shit ton of lc.
assuming isolation of course more is faster but... blah.
pointless imo.
it only takes a few drops.
but gallon jars are the tits.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 1 hour, 56 minutes
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Re: LC lid [Re: rxb]
#21735907 - 05/29/15 07:57 AM (8 years, 7 months ago) |
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i dont believe those jars are wide mouth (76mm) since it says they take a 110mm sfd.
so thats back to the drawing board.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
Edited by rxb (05/29/15 07:59 AM)
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
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Re: LC lid [Re: rxb]
#21735917 - 05/29/15 08:01 AM (8 years, 7 months ago) |
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The lids on those gallon jars are 125mm. Wide mouth quart jars, and half pint wide mouth media closures are 90mm. HUGE difference.
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rxb
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yeah i think thats what he is after. gallon jars which he can buy lids from out-grow for.
maybe i misunderstood him.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
hamloaf said: You are correct in your logic of the more LC you inoculate your substrate with the faster colonization will take place.
I must agree with the above. When I was first starting with LC I would only use a half cc or so as it was what was being touted at the time. But even with a vigorous shake it would never cover as much ground as I wanted initially and recovery was often weak. If you only made a pint of LC a half cc would be enough to inoculate 946 quarts. Personally that would be more than I could need and I would simply just end up pitching most of it (I don't like to keep LC around, even in the fridge).
So I am generous with it. I see better distribution and faster recovery.
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rxb
n00b-sabot



Registered: 08/24/13
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i feel like i must either be ok with a few days... or that im not having the same experience as you guys.
shrug
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: LC lid [Re: rxb]
#21736048 - 05/29/15 08:55 AM (8 years, 7 months ago) |
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Are you using a thick lc?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: LC lid [Re: rxb]
#21736075 - 05/29/15 09:10 AM (8 years, 7 months ago) |
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I have yet to see anyone post a grow log where a few drops or even a cc could be shaken throughout a jar and have full colonization a day or two past recovery. I know for myself I can't get it to work like that. But if I used 5cc and shake well recovery happens in two days and two days later its done. If you can't see full colonization in 5-7 days with LC its not worth the risk of using it IMO.
If you're able to do better than that, a grow log detailing the process would be of great value.
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rxb
n00b-sabot



Registered: 08/24/13
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from spores to full is 9 days. from lc to full is 4 to 6 ..
lc = shake every 2 days
spores = shake every 3.
its 3 shakes to full colonization
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: LC lid [Re: rxb] 1
#21736155 - 05/29/15 09:52 AM (8 years, 7 months ago) |
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I call shenanigans. Can't go from spores to full in 9 days
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
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Re: LC lid [Re: Ghatti]
#21736161 - 05/29/15 09:54 AM (8 years, 7 months ago) |
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uhm... yes you can
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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