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Invisiblemush madness
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Registered: 05/22/15
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Loc: Brazil
Isolating genetics?
    #21734138 - 05/28/15 08:38 PM (8 years, 7 months ago)

When starting with spores how many isolated agar plates should one start with before fruiting each one for the best chances of finding decent genetics ?

I ask because the more I read the more I find people say that finding good genetics is difficult and takes time, so what would you recommend a good place to start.


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InvisibleUncleFester
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Re: Isolating genetics? [Re: mush madness]
    #21734805 - 05/28/15 10:56 PM (8 years, 7 months ago)

Spores to agar don't usually work well, I was on a thread recently on this and I as well suffering the same problem. One guy said to dip a clean cottom swab in the spore solution and run it around the circumference of the plate. I'm not sure about isolating genetics so I can't help you out much there but I always just cut the best looking wedges of agar, so far everything is good for me


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:pm:Looking for Penis Envy & Psilocybe Cubensis Cuba spores.:pm:


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OfflineZuto18
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Re: Isolating genetics? [Re: UncleFester]
    #21734905 - 05/28/15 11:17 PM (8 years, 7 months ago)

Grow mushrooms from spores take tissue from your two best musrooms put to agar put tissue sample a close to sample b the mycelium from each sample will mate and share their genetic information creating the new better stronger faster sample c at least i think thats how it works as far as i can tell...


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Ever make mistakes in life? Lets make them birds, yeah, they are birds now. -Bob Ross
I sense a fungus among us... -me when I'm trippin'


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InvisibleLocN9ne
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Re: Isolating genetics? [Re: Zuto18]
    #21734985 - 05/28/15 11:40 PM (8 years, 7 months ago)

Quote:

Zuto18 said:
Grow mushrooms from spores take tissue from your two best musrooms put to agar put tissue sample a close to sample b the mycelium from each sample will mate and share their genetic information creating the new better stronger faster sample c at least i think thats how it works as far as i can tell...




Oh man you made that sound exciting!!!


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Q&A
US vs. THEM

The more I learn, the less I know.


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OfflineMycoBrainz
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Re: Isolating genetics? [Re: LocN9ne]
    #21735076 - 05/29/15 12:06 AM (8 years, 7 months ago)

I would do your 10-20+ best mushrooms until you have grown out and found a prolific producer.


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:stoned:


PFC x Creeper


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OfflineZuto18
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Re: Isolating genetics? [Re: LocN9ne]
    #21735119 - 05/29/15 12:18 AM (8 years, 7 months ago)

Quote:

LocN9ne said:
Quote:

Zuto18 said:
Grow mushrooms from spores take tissue from your two best musrooms put to agar put tissue sample a close to sample b the mycelium from each sample will mate and share their genetic information creating the new better stronger faster sample c at least i think thats how it works as far as i can tell...




Oh man you made that sound exciting!!!




Haha its an exiting hobby i cant wait till i get full fledged mushrooms i have some really good ideas first im gonna mate the most agressive mycelium then once i cet really nice agressive myc im gonna start fruiting them and mate only the most prolific fruiters and then im gonna take a 1g sample of each mushroom and mate only the ones that give me the most prolific expirence so basicaly im gonna make super shrooms


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Ever make mistakes in life? Lets make them birds, yeah, they are birds now. -Bob Ross
I sense a fungus among us... -me when I'm trippin'


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OfflineAchillita
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Re: Isolating genetics? [Re: Zuto18]
    #21735124 - 05/29/15 12:19 AM (8 years, 7 months ago)

Spores to agar isn't really the best way to find an isolate, but it is fairly good for expanding a culture, and a good alternative to g2g. Also for making sure the culture is sterile, and has no contams.

A lot of very experienced growers on here say that clones are usually better than isolates. If you fruit a MS, and get a bunch of clusters or very potent fruits, cloning a piece of the mushroom tissue would allow you to have the exact same potency or the sae fruiting rate(hopefully). To get both of those is the hardest part.
Quote:

Zuto18 said:
Grow mushrooms from spores take tissue from your two best musrooms put to agar put tissue sample a close to sample b the mycelium from each sample will mate and share their genetic information creating the new better stronger faster sample c at least i think thats how it works as far as i can tell...



Well, kinda-ish. If a clone is put next to a clone, it won't share genetic information and "mate". It'll just grow and work together.

To actually get mycelium to mate, you'd need 2 tortomase(non-rhizomorphic and most likely won't fruit) isolates. WHen they go together, they'd mate and make a strain. Most clones aren't true isolates, but work completely fine and better than isolates sometimes. Genetics at spores would usually dictate whether or not how fast the mycelium grows, potency, size of mushrooms, or how many mushrooms will fruit together. When you clone or isolate, you pick out the traits you want and try and get an isolate or clone that has all of the traits you want, or at least the ones most important.


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OfflineAchillita
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Re: Isolating genetics? [Re: Zuto18]
    #21735131 - 05/29/15 12:21 AM (8 years, 7 months ago)

Quote:

Zuto18 said:
Quote:

LocN9ne said:
Quote:

Zuto18 said:
Grow mushrooms from spores take tissue from your two best musrooms put to agar put tissue sample a close to sample b the mycelium from each sample will mate and share their genetic information creating the new better stronger faster sample c at least i think thats how it works as far as i can tell...




Oh man you made that sound exciting!!!




Haha its an exiting hobby i cant wait till i get full fledged mushrooms i have some really good ideas first im gonna mate the most agressive mycelium then once i cet really nice agressive myc im gonna start fruiting them and mate only the most prolific fruiters and then im gonna take a 1g sample of each mushroom and mate only the ones that give me the most prolific expirence so basicaly im gonna make super shrooms



Sorry zuto, don't mean to rain on your parade but mushrooms don't work like that.:sad: It'd be easier if they did though, check out my long ass post for more info. :sad:


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OfflineZuto18
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Re: Isolating genetics? [Re: Achillita]
    #21735247 - 05/29/15 01:06 AM (8 years, 7 months ago)

Ah well dont feel like your raining on my parade im glad you corrected me thanks for that =) now at least i wont do somthing stupid but im definatley gonna try my hand at isolating genomes and the like sometime it sounds like alot of fun and very rewarding i guess i need to read more xD


--------------------

Ever make mistakes in life? Lets make them birds, yeah, they are birds now. -Bob Ross
I sense a fungus among us... -me when I'm trippin'


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OfflineAchillita
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Re: Isolating genetics? [Re: Zuto18]
    #21735258 - 05/29/15 01:12 AM (8 years, 7 months ago)

You're welcome man, reading is your best bet. I'd suggest to learn how mushrooms mate from spores. It's kinda complex, but can be simplified. There's tons of info on this site.

Good luck at isolating and taking clones. Oh and taking clones and trying to isolate from there is much easier and faster than trying to isolate straight from spores.


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OfflineZuto18
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Re: Isolating genetics? [Re: Achillita]
    #21735427 - 05/29/15 02:20 AM (8 years, 7 months ago)

If i can get it down my goal is create my own albino strain but first to learn im probably gonna try working on albino a+ and see if i can get some nice albinos from that but first things first i gotta finish my current pf cakes and once thats down ill invest in a pc and get good at bulk then comes playing with genomes =)


--------------------

Ever make mistakes in life? Lets make them birds, yeah, they are birds now. -Bob Ross
I sense a fungus among us... -me when I'm trippin'


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Invisiblemush madness
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Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: Isolating genetics? [Re: Zuto18]
    #21736018 - 05/29/15 08:43 AM (8 years, 7 months ago)

Thanks guys, I had been working directly with spores and on the third transfer but I will work with pins in the future. I guess something that is proven to fruit is better to start with than something that's not

This wasn't a total loss I did get some great agar experience,  a lot easier and not as intimidating as one would think. Alot of fun actually


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Offlinespacechildo
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Re: Isolating genetics? [Re: mush madness]
    #21736108 - 05/29/15 09:29 AM (8 years, 7 months ago)

Quote:

mush madness said:
This wasn't a total loss I did get some great agar experience




and a clean culture on agar that you now can put to grain! :laugh:


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Invisiblemush madness
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Registered: 05/22/15
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Re: Isolating genetics? [Re: spacechildo]
    #21736187 - 05/29/15 10:07 AM (8 years, 7 months ago)

Quote:

spacechildo said:
Quote:

mush madness said:
This wasn't a total loss I did get some great agar experience




and a clean culture on agar that you now can put to grain! :laugh:




I will try a few of them in pints in the future,  hell I might get lucky


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Offlinespacechildo
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Re: Isolating genetics? [Re: mush madness]
    #21736200 - 05/29/15 10:12 AM (8 years, 7 months ago)

lucky?

you dont need an isolate to grow mushrooms man, once you clean up a culture on agar its ready to go!
isolation is more for knowing what you get each and every time in terms of potency, size, yields etc.


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Invisiblemush madness
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Registered: 05/22/15
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Re: Isolating genetics? [Re: spacechildo]
    #21736368 - 05/29/15 11:03 AM (8 years, 7 months ago)

Quote:

spacechildo said:
lucky?

you dont need an isolate to grow mushrooms man, once you clean up a culture on agar its ready to go!
isolation is more for knowing what you get each and every time in terms of potency, size, yields etc.




That's what I mean,  lucky to find one that has everything that I am looking for.


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Invisible36fuckin5
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Re: Isolating genetics? [Re: mush madness]
    #21736958 - 05/29/15 01:59 PM (8 years, 7 months ago)

You can absolutely start from spores and get a good isolate. It's the best way to find a culture. It's also the most time-consuming.

Start with a tiny swipe of spores on each plate. Label everything. I like to do letters for the agar plate so the 4th transfer might be like CFAD, coming from plate C with spores, plate F on the first transfer, A on the third and D on the fourth.

Once you've isolated a bunch of strains, make a backup slant of each and every one of them. Then take another wedge from the same plate and throw it on grain. Again, label everything.

Do a shitload of tiny grows at once and see which one does best. Keep that one and any others that look promising, toss the rest.

Go back to the slants of the ones you like and make another round of plates. Make several slants of each strain using wood in the tube for long-term backup. Take the rest of the plate and make up grain jars.

Like I said, it can be done and it's the best way. But you're gonna have to do a bunch of shitty strains to find your good one. Luckily Cubensis generally does reasonably well even with shitty strains.


--------------------
Redd Foxx said:
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i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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