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OfflineKeyaladis
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Hello *DELETED* *DELETED* *DELETED*
    #21733737 - 05/28/15 06:41 PM (8 years, 8 months ago)

Post deleted by Keyaladis

Reason for deletion: Ayy


Edited by Keyaladis (04/16/18 11:17 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Is my LQ containated? [Re: Keyaladis]
    #21733769 - 05/28/15 06:52 PM (8 years, 8 months ago)

test it


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InvisibleFriedEggS
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Re: Is my LQ containated? [Re: bodhisatta]
    #21733858 - 05/28/15 07:16 PM (8 years, 8 months ago)

:whathesaid:


It's probably impossible to tell if a liquid "qulture" :tongue2: is safe by looking at it.  Even harder trying to tell from looking at a pic.

But we can probably guess if you give us some more info. :smile: :thumbup: What was it inoculated with, in what conditions, and what kind of filter are you using on that jar? (pics of filter?) Has the filter ever gotten wet?


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(Yes, the egg is real)

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OfflineKeyaladis
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Re: Is my LQ containated? *DELETED* [Re: FriedEgg]
    #21734000 - 05/28/15 08:05 PM (8 years, 7 months ago)

Post deleted by Keyaladis

Reason for deletion: Reasons


Edited by Keyaladis (05/28/15 08:48 PM)


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Offlinehamloaf
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Re: Is my LQ containated? [Re: Keyaladis]
    #21735709 - 05/29/15 06:07 AM (8 years, 7 months ago)

Yea, that LC doesn't look good.  NOT being able to see through the liquid media is a symptom of a visually contaminated LC.  Wouldn't even expand on that culture.  Plus, inoculating LC with spores is the correct way to begin a LC with one foot in the grave.  Use your spores to inoculate agar.  Once you have a clean culture on agar, use the clean agar culture to inoculate LC with using the wedge, and transfer method.

Visual signs that your LC is contaminated.
https://www.shroomery.org/forums/showflat.php/Number/21068298#21068298


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How I Get Stuff done. - My Reference Guide. - My Grows.


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Offlineblackout
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Re: Is my LQ containated? [Re: hamloaf]
    #21739833 - 05/30/15 05:03 AM (8 years, 7 months ago)

What is it made from? honey will have bits in it which can look like that.


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OfflineFerather
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Re: Is my LQ containated? [Re: blackout]
    #21740160 - 05/30/15 08:38 AM (8 years, 7 months ago)

Ok, I'm gonna say from experience. DON'T use honey. Its utterly terrible. Chances of starting with a arsenal of either contamination or fungicides is too high.
Not worth working with, not worth the money. Try brewers sugar (glucose, dextrose). Its cheap and clear.

Yeast is a fungus, brewers use yeast. Hint hint :smile:


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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InvisiblebodhisattaMDiscordReddit
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Re: Is my LQ containated? [Re: Ferather]
    #21740172 - 05/30/15 08:44 AM (8 years, 7 months ago)

make YPD broth or malt extract broth. Agar without the agar. plain sugar seems to work but there's no free nitrogen or amino acids.


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OfflineFerather
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Re: Is my LQ containated? [Re: bodhisatta]
    #21740192 - 05/30/15 08:51 AM (8 years, 7 months ago)

To my understanding brewers sugar is 90% sugars and 10% other, most likely nutrition for the yeast to grow.
Its man made so its likely to fit purpose. As I said yeast is a fungus.
It's also made to be clear for inspection and detection.

I have no experience with other solubles. Except SPF and sucrose.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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InvisiblebodhisattaMDiscordReddit
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Re: Is my LQ containated? [Re: Ferather]
    #21740213 - 05/30/15 08:59 AM (8 years, 7 months ago)

brewers sugar is usually corn sugar for bottle priming. it's straight dextrose/glucose

the yeast are already grown you bottle condition with active yeast that has enough glycogen and trehalose to carry out bottle conditioning requirements, if you don't filter the autolyzed yeast from primary fermentation is plenty food too.

sucrose is table sugar.

There's some products that are made to be like sugar + DME but that's all weird homebrew shit.


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InvisiblebodhisattaMDiscordReddit
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Re: Is my LQ containated? [Re: bodhisatta]
    #21740248 - 05/30/15 09:10 AM (8 years, 7 months ago)

growing yeast for brewing isn't as easy as you would think. it's far more easy to keep fermentations moving along and reusing and possibly washing the yeast. you can do that for 20 generations if you're very clean and your handling techniques are spot on every time

even doing that most breweries run into rampant contamination and find themselves buying new pitches or using dry yeast eventually. it's really not that easy to get your shit together well enough without a dedicated full time lab department and well trained cellermen and brewers.



wild yeast like this canada albicans species(yeast infection quite literally) can show up as well as plenty of anaerobic bacteria
if you look closely you can see a pseudo hyphae in the C.albicans it's an interesting species of yeast as it can be both yeast like and "Fungus like" it has yeast, pseudo hyphae, and actual hyphae morphologies depending.



bacteria are a real problem too, they're much easier to grow and keep around than yeast.



the requirements for good yeast growth to get counts getting above 109 cells per mL are stringent, during propagation you use oxygen for cell wall synthesis. most breweries cant handle self sustained yeast propagation you have to get into the 100,000+ bbl/year range for most breweries to get to the point of being able to get that program going. 

I highly suspect the quality of LC varies hugely among hobby growers here on the shroomery due to LC growing conditions, DO, and nutrient contents. either way the result is if you put any living mycelium in a jar it will take off.


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Offlinepinto
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Registered: 02/23/15
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Re: Is my LQ containated? [Re: bodhisatta]
    #21740622 - 05/30/15 11:00 AM (8 years, 7 months ago)

This! :thumbup:
-from a professional brewer


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Stupid Sexy Flanders.


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Offlinehamloaf
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Re: Is my LQ containated? [Re: pinto]
    #21740921 - 05/30/15 12:37 PM (8 years, 7 months ago)

Brewing beer, and mushroom cultivation are two COMPLETELY different fields of expertise, therefore, are held to completely different stands of quality. 
End of boil cycle grain water makes a better LC than malt extract, and any form of sugars combined. 
https://www.shroomery.org/forums/showflat.php/Number/20970238#20970238
https://www.shroomery.org/forums/showflat.php/Number/21627998#21627998


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisiblebodhisattaMDiscordReddit
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Re: Is my LQ containated? [Re: hamloaf]
    #21740947 - 05/30/15 12:43 PM (8 years, 7 months ago)

end of boil grain water flash evaporated is malt extract with higher sugar to starch ratio than you get from rye which would be higher starch to sugar un-malted. either way if made with grain, grain extract or the likes it's better than straight sugar or honey or karo.


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InvisibleGhatti
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Re: Is my LQ containated? [Re: bodhisatta]
    #21741000 - 05/30/15 12:59 PM (8 years, 7 months ago)

LME for the win!


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OfflineHaywire
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Re: Is my LQ containated? [Re: Ghatti]
    #21741068 - 05/30/15 01:20 PM (8 years, 7 months ago)

I'm going to try that grain water LC next time.
atm i've got two jars, one with LME the other with honey.
LME is far ahead.


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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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Offlineblackout
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Re: Is my LQ containated? [Re: Haywire]
    #21741087 - 05/30/15 01:26 PM (8 years, 7 months ago)

Quote:

Haywire said:
atm i've got two jars, one with LME the other with honey.
LME is far ahead.



Next time try a LME with low concentration. Workman from sporeworks recommends just 0.1-0.5% and I find it works great.

If using honey I would recommend boiling it, letting it settle in the fridge and decanting it off any sediments and then heat treat it again.


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OfflineHaywire
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Re: Is my LQ containated? [Re: blackout]
    #21741177 - 05/30/15 01:53 PM (8 years, 7 months ago)

Quote:

blackout said:
Quote:

Haywire said:
atm i've got two jars, one with LME the other with honey.
LME is far ahead.



Next time try a LME with low concentration. Workman from sporeworks recommends just 0.1-0.5% and I find it works great.

If using honey I would recommend boiling it, letting it settle in the fridge and decanting it off any sediments and then heat treat it again.




hmm, I'm not sure what concentration I did. I put a teaspoon of LME in 200 ml of water. I'll definitely try it next time.
good tip for the honey, I'll stick with LME for now, but you never now when you might need it.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineKeyaladis
Kin

Registered: 05/28/15
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Re: Is my LQ containated? *DELETED* [Re: Haywire]
    #21744455 - 05/31/15 11:14 AM (8 years, 7 months ago)

Post deleted by Keyaladis

Reason for deletion: Reasons


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OfflineHaywire
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Re: Is my LQ containated? [Re: Keyaladis]
    #21744551 - 05/31/15 11:51 AM (8 years, 7 months ago)

This is what mine looks like.



--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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