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jellycrab
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12 days after Inoculation and 1 jar has no signs of growth
#21732073 - 05/28/15 10:52 AM (8 years, 8 months ago) |
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Its been 12 days since I inoculated 12 brf jars (spores from trusted sponsor). First sign of growth was at the 3rd day. All but one jar have signs of growth and most of those jars only show growth on 1 or 2 inoculation points the best jars show growth at 3 points but none show growth at all 4 points. Was wondering if this is common. This is only my second grow and would appreciate feedback. P.S. I only shook syringes before inoculating I didn't shake between jars (maybe that has something to do with it)
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
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Aero
Orea


Registered: 11/01/13
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21732084 - 05/28/15 10:55 AM (8 years, 8 months ago) |
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Quote:
jellycrab said: Its been 12 days since I inoculated 12 brf jars (spores from trusted sponsor). First sign of growth was at the 3rd day. All but one jar have signs of growth and most of those jars only show growth on 1 or 2 inoculation points the best jars show growth at 3 points but none show growth at all 4 points. Was wondering if this is common. This is only my second grow and would appreciate feedback. P.S. I only shook syringes before inoculating I didn't shake between jars (maybe that has something to do with it)
happens,
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: Aero]
#21732157 - 05/28/15 11:15 AM (8 years, 8 months ago) |
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Should I expect to eventually have growth on all 4 inoculation points or is it common for some inoculation points to never germinate?
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
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UncleFester
Lord Moldy-Butt



Registered: 09/23/14
Posts: 460
Loc: Where The Sun Don't Shine...
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21732177 - 05/28/15 11:23 AM (8 years, 8 months ago) |
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Sometimes it takes as much as two weeks. I had a homemade syringe that took almost 3 weeks to germinate. Just be patient bro spores take their sweet time, but once you cut off a corner wedge of clean brf/verm and transfer it to a simple easily broken grain like popcorn in a still air box or glove box. Remember to keep it sterile!
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Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: UncleFester]
#21733902 - 05/28/15 07:31 PM (8 years, 8 months ago) |
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Im just hoping its not a bacterial contaminate. (sniffed the jars no bad smell)
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
Edited by jellycrab (05/28/15 07:32 PM)
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UncleFester
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21741431 - 05/30/15 03:05 PM (8 years, 7 months ago) |
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Dude what... Why would you open it are you nuts? Do you want a contamination?
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Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: UncleFester]
#21741498 - 05/30/15 03:18 PM (8 years, 7 months ago) |
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I never said I opened it I just sniffed over lid. Im not retarded lol
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21741542 - 05/30/15 03:30 PM (8 years, 7 months ago) |
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Update: On one of the jars with only one spot of growth now shows growth at another inoculation point. Im thinking that I used too much MS I didn't really measure closely just injected a little in each hole but there where times when substrate got stuck in needle and made me squirt too much. Would that cause delayed germination?
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
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Aero
Orea


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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21741637 - 05/30/15 04:04 PM (8 years, 7 months ago) |
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Quote:
jellycrab said: Update: On one of the jars with only one spot of growth now shows growth at another inoculation point. Im thinking that I used too much MS I didn't really measure closely just injected a little in each hole but there where times when substrate got stuck in needle and made me squirt too much. Would that cause delayed germination?
not really
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UncleFester
Lord Moldy-Butt



Registered: 09/23/14
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21744542 - 05/31/15 11:47 AM (8 years, 7 months ago) |
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Quote:
jellycrab said: I never said I opened it I just sniffed over lid. Im not retarded lol
oh ok you had me like pls
But inoculation is the most important part after sterilization so you gotta be more careful dude. Know how much you use. Plan your movements in the sab/govebox
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Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: UncleFester]
#21744866 - 05/31/15 01:39 PM (8 years, 7 months ago) |
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So its 15 days after inoculation and 6 jars are about 90% 5 jars that are about 30% (the ones with growth on only 1 inoculation point) and 1 jar with no growth. How long should I wait before tossing the jar with no growth?
Edited by jellycrab (05/31/15 01:45 PM)
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Aero
Orea


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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21744919 - 05/31/15 02:03 PM (8 years, 7 months ago) |
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if u want to wait wait, if u want to knock up a few more jars then use the one that didn't show any growth
we are talking about 91.66666666666666% success rate here so get over it
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UncleFester
Lord Moldy-Butt



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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21745005 - 05/31/15 02:28 PM (8 years, 7 months ago) |
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I'd say after a month of no growth toss it
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Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: Aero]
#21745029 - 05/31/15 02:34 PM (8 years, 7 months ago) |
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I don't have any more spores so Ill prob just wait another week and if i still see no growth ill chuck it. Also was planing on doing the Poor Man's Mycelium Syringe Tek: http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek to inoculate more jars going to do grain jars (rye) cause I got a pc so I'm gonna finally be able to a bulk grow!!
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
Edited by jellycrab (05/31/15 02:39 PM)
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UncleFester
Lord Moldy-Butt



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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21745097 - 05/31/15 03:04 PM (8 years, 7 months ago) |
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I think that's a little too much work to keep mycelium running dude. What I did was just cut off a clean corner wedge of the cake (at base of jar pretty much) then I transferred it to popcorn (or any other grain duh) in a still air box. This is the most stress free thing I'd had to do. You can also drop it like it's hot on some agar (I recommend: Pastywhyte's easy agar tek). Just remember to flame sterilize the cutting tool with a alcohol lamp
Turn that jar into a mycelium bank and keep it alive

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Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
Edited by UncleFester (05/31/15 03:15 PM)
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: UncleFester]
#21745128 - 05/31/15 03:15 PM (8 years, 7 months ago) |
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I don't think it sounds like a lot of work all you have to do is inject jars with sterilized Karo and then let sit a couple minutes than draw back the Karo than you get 10cc of lc enough to inoculate plenty of jars and you don't have to cut anything. I guess I could just wait till I get my first flush and clone with agar.
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
Edited by jellycrab (05/31/15 03:20 PM)
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21745436 - 05/31/15 04:35 PM (8 years, 7 months ago) |
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Instead of Karo Ill prob use distilled water
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
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UncleFester
Lord Moldy-Butt



Registered: 09/23/14
Posts: 460
Loc: Where The Sun Don't Shine...
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21745647 - 05/31/15 05:47 PM (8 years, 7 months ago) |
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Distilled water does sound better but remember to sterilize it. And I'm just kinda lazy so I don't feel like putting too much though in how to keep it going. But hey its worth a try dude
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Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: UncleFester]
#21748343 - 06/01/15 12:46 PM (8 years, 7 months ago) |
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If I do bulk my next grow what grain would you recommend. its between wbs and rye.
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
Edited by jellycrab (06/01/15 01:25 PM)
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UncleFester
Lord Moldy-Butt



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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21748530 - 06/01/15 01:30 PM (8 years, 7 months ago) |
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I've had 6 month old syringe(s) arrive and kept in a fridge for 3 more months and still worked just fine. IME the one that works, works best IfyouknowwhatImean.jpg

I highly doubt a trusted sites would give out bad syringes. Maybe it was your procedure/handling of the syringe.
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Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
Edited by UncleFester (06/01/15 01:31 PM)
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: UncleFester]
#21748902 - 06/01/15 02:45 PM (8 years, 7 months ago) |
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My procedure was I shook up syringe than flame sterilized before every jar and when I injected I made sure it was next to the glass. Now their where times when sub got in needle and made me squirt more than I intended but if anything that would speed up colonization. When I prepared the brf jars I followed standard recipe and when I squeezed it in my hand no drops came out just a little moisture so I don't think their too moist. And I filled the jars loosely.
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
Edited by jellycrab (06/01/15 02:53 PM)
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UncleFester
Lord Moldy-Butt



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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: jellycrab]
#21749211 - 06/01/15 04:06 PM (8 years, 7 months ago) |
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Yeah that's seems on point enough for me dude.. Hmm I'd say just wait it out till it's been a month and if no growth then PC it and try again, you can reuse uncolonized sub as long as it's not contaminated
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Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
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jellycrab
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Re: 12 days after Inoculation and 1 jar has no signs of growth [Re: UncleFester]
#21750219 - 06/01/15 08:10 PM (8 years, 7 months ago) |
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I was checking my jars today and when I went to pick one up and set it back down some of the dry verm fell (the bottom of the layer, it apears the top of verm barrier is still intact). do you think Ill get a contaminate? the dry verm fell where there was no colonization so im worried. Im definitely not gonna mess with that jar anymore
-------------------- "I used to have a drug problem…..now I make enough money." … David Lee Roth
Edited by jellycrab (06/01/15 08:26 PM)
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