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OfflineLyghtsout
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Registered: 10/18/13
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Truffles seem to be taking a long time to colonize
    #21730732 - 05/27/15 11:35 PM (8 years, 8 months ago)

I'm pretty sure its genetics just want to hear your thoughts. They seem to he colonizing slow is there anything I can do to stimulate growth? Such as, injecting steroids into the mycelium while playing lamb of god? Also just general feedback on how they're looking they were inoculated 2 months ago I feel like I should be seeing invirto growth by now but have never grown truffles before. Its odd but the P. Tampensis jars are colonizing faster then the P. Mexicana?




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Invisiblestevo

Registered: 04/11/05
Posts: 5,100
Re: Truffles seem to be taking a long time to colonize *DELETED* [Re: Lyghtsout]
    #21731115 - 05/28/15 02:42 AM (8 years, 8 months ago)

Post deleted by stevo

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OfflineLyghtsout
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Re: Truffles seem to be taking a long time to colonize [Re: stevo]
    #21763010 - 06/04/15 07:52 PM (8 years, 7 months ago)

They're rye grain berries. I'm wondering if that looks like normal progress for 2 months? I read somewhere that some people eat them after 2 months, but these don't even look like anything more then mycelium.


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InvisibleCplCauliflower
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Re: Truffles seem to be taking a long time to colonize [Re: Lyghtsout]
    #21763969 - 06/04/15 11:31 PM (8 years, 7 months ago)

Maybe you could try spawning them to a monotub? All you gotta do is pick up some coco coir, verm, peat moss, and hydrated lime and you're good to go for an amazing tub. Just prepare it Damions way (http://www.shroomery.org/forums/showflat.php/Number/11916595), maybe use one preparation for both tubs if you only have 1 qt of each. Just wait another month or so and spawn them to a mono, let them colonize, case it, and then fruit it.


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InvisibleLocN9ne
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Re: Truffles seem to be taking a long time to colonize [Re: CplCauliflower]
    #21764049 - 06/04/15 11:55 PM (8 years, 7 months ago)

Is that green or like a grey down by your finger 2nd pic? Hard to tell in pictures.


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InvisibleRPW
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Re: Truffles seem to be taking a long time to colonize [Re: CplCauliflower]
    #21764499 - 06/05/15 03:38 AM (8 years, 7 months ago)

Quote:

CplCauliflower said:
Maybe you could try spawning them to a monotub? All you gotta do is pick up some coco coir, verm, peat moss, and hydrated lime and you're good to go for an amazing tub. Just prepare it Damions way (http://www.shroomery.org/forums/showflat.php/Number/11916595), maybe use one preparation for both tubs if you only have 1 qt of each. Just wait another month or so and spawn them to a mono, let them colonize, case it, and then fruit it.




He's trying to grow sclerotia, not fruit them out and grow mushrooms.


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InvisibleGuardian187
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Re: Truffles seem to be taking a long time to colonize [Re: RPW]
    #21764737 - 06/05/15 06:42 AM (8 years, 7 months ago)

I had some P. Galindoi that took 2.5 months to start stone production. I didn't think they would ever start! I've also heard multiple instances of Mexicana not producing stones.


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OfflineLyghtsout
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Re: Truffles seem to be taking a long time to colonize [Re: Guardian187]
    #21827035 - 06/19/15 07:46 AM (8 years, 7 months ago)




I'm getting frustrated as hell :p this is the jars close to 3 months they're stalled. What can I do to stimulate growth? I considered placing them in sunlight for a few days (they're in the dark) and-or breaking up the mycelium? I have 24 jars Im not spawning going for sclerotia production


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OfflineLyghtsout
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Re: Truffles seem to be taking a long time to colonize [Re: Lyghtsout]
    #21827070 - 06/19/15 07:56 AM (8 years, 7 months ago)

Another quick question, I am starting to think they may not have FAE, would loosing the lids to allow higher FAE be OK at this stage? They already have 0.22µm gas exchange filters. And if I can stimulate growth somehow these jars should still have time to grow before going "bad"? Or should I toss them?

Reasons I don't believe it to be a contamination - of the 24 jars 6 are tampensis, while they stalled they got a lot further in colonization and show no signs of contamination. All of the tampensis got a lot further which implies to me that genetics has somewhat of a role. Also, I've colonized tons of jars and have a very low contamination rate, before this project it was 1/36 to give you an idea.


Edited by Lyghtsout (06/19/15 08:29 AM)


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Invisiblestevo

Registered: 04/11/05
Posts: 5,100
Re: Truffles seem to be taking a long time to colonize *DELETED* [Re: Lyghtsout]
    #21827214 - 06/19/15 08:44 AM (8 years, 7 months ago)

Post deleted by stevo

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Invisiblemush madness
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Re: Truffles seem to be taking a long time to colonize [Re: stevo]
    #21827438 - 06/19/15 10:00 AM (8 years, 7 months ago)



This jar is alt 7 but you can see the thread like mycelium crawling up the glass and the stones forming well above the oat grain as the last poster mentioned ,  this jar is a isolate but with 24 jars you should have stones forming.  I normally get them within 3 weeks.

I would have to agree that fae is probably your issue,  I would swap out the air filters that you have for synthetic filter disc or put them all in a mono tub.


Edited by mush madness (06/19/15 10:02 AM)


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OfflineLyghtsout
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Re: Truffles seem to be taking a long time to colonize [Re: mush madness]
    #21827780 - 06/19/15 11:27 AM (8 years, 7 months ago)

How will I swap the filters already on them? Would loosening the lids slightly or drilling a hole (1/8") be ok?


Edited by Lyghtsout (06/19/15 11:34 AM)


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Invisiblemush madness
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Re: Truffles seem to be taking a long time to colonize [Re: Lyghtsout]
    #21827803 - 06/19/15 11:35 AM (8 years, 7 months ago)

In a sab remove the filter loosen the ring and change out for a synthetic filter disc


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InvisibleGrey
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Re: Truffles seem to be taking a long time to colonize [Re: mush madness]
    #21828041 - 06/19/15 12:47 PM (8 years, 7 months ago)

Are you positive it's stone producer mycelium?


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OfflineLyghtsout
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Re: Truffles seem to be taking a long time to colonize [Re: mush madness]
    #21831051 - 06/20/15 01:36 AM (8 years, 7 months ago)

I'm positive they're sclerotia producing strains, they aren't even fully colonized and I believe they won't start producing sclerotia until they feel there's no more substrate to colonize.

About changing the filters, is it pretty easy to do/straight forward if I mess with them? I actually bought these jars from a sponsor so I have no idea how the filter that's already on goes together. Is it as simple as yanking out the filter then taping the disc over the hole?  I'll shoot the sponsor I purchased them from to explain my situation and inquire more information.

These jars are already 3.5 months old and were inoculated 3 months ago, after changing out the filter - assuming they start colonizing at a normal rate - I should be able to get sclerotia from them before the jars go bad right? I'd most likely give them another 3 months if they start colonizing correct.

Also, is just loosening the lids a tad bit be a bad idea? Will that welcome contaminations? The reason I ask is it just seems way simpler and more effective, I'm not experienced with rye berries without a vermiculite layer, but wouldn't the fact that they're already partly colonized make them more resistant to contamination?


Edited by Lyghtsout (06/20/15 01:45 AM)


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OfflineLyghtsout
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Re: Truffles seem to be taking a long time to colonize [Re: Lyghtsout]
    #21839413 - 06/21/15 09:33 PM (8 years, 7 months ago)

Just a update, I slightly loosened the lid on one of the jars to see what happened. Also, I broke up the mycelium in a jar by hitting it against a tire. The inside of the jar seems to not have been colonized at all, when it was 30 percent colonized I merely shook the jars. I'm starting to think the reason there isn't enough FAE is because the mycelium colonized to form a wall around the substrate suffocating the center, it makes sense that now with it broken up more air will be allowed to get into the substrate.

I will update in a week to let y'all know what happened, in the mean time, any input is appreciated.


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InvisibleJuiceh
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Re: Truffles seem to be taking a long time to colonize [Re: Lyghtsout]
    #21841101 - 06/22/15 09:54 AM (8 years, 7 months ago)

It's not FAE that is the issue, it's GE. Those syringe filters suck for grains. They are great for LCs tho. Make a bunch of new lids with a SFD siliconed over a 1/4" hole. Don't take apart the ones you have, save them for LC. Take your new lids, once silicone is cured, and wrap them all in foil and PC them. Then in a SAB or in front of your flow hood swap out the lids with the new ones, cracking them out of the foil just before applying them.


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Edited by Juiceh (06/22/15 09:56 AM)


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OfflineLyghtsout
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Re: Truffles seem to be taking a long time to colonize [Re: Juiceh]
    #21841988 - 06/22/15 03:04 PM (8 years, 7 months ago)

Thank you! This is informative! I contacted the shroomery sponsor and they said they've done away with the style of filters on the jars I bought back in February 25th and they're sending me replacement filters free of charge. The jars should still be good to produce sclerotia right? I inoculated on March 16th, I've heard of people letting the jars sit quite a while before they go bad, my main concern is if they've dried up.


Edited by Lyghtsout (06/22/15 03:05 PM)


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InvisibleJuiceh
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Re: Truffles seem to be taking a long time to colonize [Re: Lyghtsout]
    #21842055 - 06/22/15 03:20 PM (8 years, 7 months ago)

You will be fine after the filter change. A buddy of mine has some sclerotia jars that are almost 2 years old!


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Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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InvisibleFantastic Mr. Fox
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Re: Truffles seem to be taking a long time to colonize [Re: Juiceh]
    #21842232 - 06/22/15 04:22 PM (8 years, 7 months ago)

plz post pics come harvest!!!

Or shoot me a :pm:


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