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Offlinenwalker248
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Using a bleach solution to sterilize area during inoculation and making a spore syringe?
    #21730254 - 05/27/15 08:37 PM (8 years, 8 months ago)

I will soon be making a spore syringe with a spore print that I've been sent, and have chosen to do so within my bathroom as it's a reasonably small room and can be sprayed easily, in theory reducing the possibility of contams. getting in the syringe; the same environment will be used for inoculation.

I'm quite stuck on what to use to clean the area however. No shops around sell rubbing alcohol, and the closest to any disinfectant in the house is dettol surface cleaner (Claiming to kill 99.9% of bacteria,) and bleach. Would these two products together be enough to create an environment clean enough to inoculate and create a spore syringe? I also have white spirit, but I don't believe that kills bacteria as its used to just strip paint.

Thanks.


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InvisibleFriedEggS
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Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: nwalker248]
    #21730287 - 05/27/15 08:49 PM (8 years, 8 months ago)

You don't need to sanitize your SAB or room.  Just spray inside the SAB with soap and water.  The point is to make the air inside the box still so nothing flies around.  If it does fly around, it'll get stuck to the wet walls of your SAB.  Just be careful about letting anything touch the walls.  Also don't lift things up if you don't have to. 

I was paranoid about it too until recently.  Things have been fine since I've been only using soap and water.  :thumbup:


edit: wait... you're going to need isopropyl alcohol no matter what you do.  the jars need to be wiped down.  what country are you in where isopropyl alcohol is not available?


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Edited by FriedEgg (05/27/15 08:52 PM)


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Offlinephilsfan
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Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: FriedEgg]
    #21730822 - 05/28/15 12:15 AM (8 years, 8 months ago)

Iso alcohol should be at any pharmacy or pharmacy aisle at grocery store, Wal-Mart, dollar store, etc.  And yes focus on using a still air box and keeping everything inside that sterile you don't want to try to sterilize a while room just close the windows and turn off any fans in the room.


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Offlinenwalker248
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Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: philsfan]
    #21731354 - 05/28/15 06:39 AM (8 years, 8 months ago)

Quote:

philsfan said:
Iso alcohol should be at any pharmacy or pharmacy aisle at grocery store, Wal-Mart, dollar store, etc.  And yes focus on using a still air box and keeping everything inside that sterile you don't want to try to sterilize a while room just close the windows and turn off any fans in the room.




Here in the UK it's pretty difficult to find apparently. Asked at asda, boots pharmacy, b&q and none could point me in the right direction.

Are there any alternatives?


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InvisiblebodhisattaMDiscordReddit
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Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: nwalker248]
    #21731361 - 05/28/15 06:42 AM (8 years, 8 months ago)

Using neither :shrug: neither sterilize they both sanitize and if you do things right you use neither of them to make prints or syringes


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InvisibleFriedEggS
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Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: bodhisatta]
    #21732048 - 05/28/15 10:42 AM (8 years, 8 months ago)

Quote:

bodhisatta said:
Using neither :shrug: neither sterilize they both sanitize and if you do things right you use neither of them to make prints or syringes



then what do you use to wipe down your hands?


Quote:

nwalker248 said:Are there any alternatives?



try buying it online


--------------------

(Yes, the egg is real)

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Offlinenwalker248
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I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: FriedEgg]
    #21732820 - 05/28/15 02:37 PM (8 years, 8 months ago)

Would alcohol swabs with ISA (Say 50 individual packets, at 70%) be enough, or should i say give off enough ISA to sterilize things for inoculation and making a spore syringe? A friend has some that he said he would give me.

Thanks.

Edit: Perhaps I could drop a bunch of them into a spray bottle of water, and make a spray solution? I'm not sure how well this would work though. The only problem is that they're only 70%.


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Edited by nwalker248 (05/28/15 02:53 PM)


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Invisibleoontribe
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Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: nwalker248]
    #21732934 - 05/28/15 03:16 PM (8 years, 8 months ago)

dont forget to PC or steam sterilize the syringe and the shot gloss before making your spore print. and dont cool them down in a fridge. my 2 cents.


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InvisibleFriedEggS
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Registered: 09/22/14
Posts: 2,536
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Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: nwalker248]
    #21733160 - 05/28/15 04:17 PM (8 years, 8 months ago)

Quote:

nwalker248 said:
Would alcohol swabs with ISA (Say 50 individual packets, at 70%) be enough, or should i say give off enough ISA to sterilize things for inoculation and making a spore syringe? A friend has some that he said he would give me.

Thanks.

Edit: Perhaps I could drop a bunch of them into a spray bottle of water, and make a spray solution? I'm not sure how well this would work though. The only problem is that they're only 70%.



whats ISA?

You're going to drive yourself mad with those little alcohol swabs. 

And I doubt that solution would work.  Why not buy isopropyl alcohol online?  You'll probably go through a lot of it.


--------------------

(Yes, the egg is real)

How to post pics


Extras: Filter Print Post Top
Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: FriedEgg]
    #21733181 - 05/28/15 04:22 PM (8 years, 8 months ago)

Quote:

FriedEgg said:
Quote:

nwalker248 said:
Would alcohol swabs with ISA (Say 50 individual packets, at 70%) be enough, or should i say give off enough ISA to sterilize things for inoculation and making a spore syringe? A friend has some that he said he would give me.

Thanks.

Edit: Perhaps I could drop a bunch of them into a spray bottle of water, and make a spray solution? I'm not sure how well this would work though. The only problem is that they're only 70%.



whats ISA?

You're going to drive yourself mad with those little alcohol swabs. 

And I doubt that solution would work.  Why not buy isopropyl alcohol online?  You'll probably go through a lot of it.




ISA = isoprop. alcohol. The only reason is that I was planning to get things started tomorrow as I have everything needed for the syringe. And ordering some in means I'll have to wait till monday. Just how unpractical are the swabs do you reckon? I'll wait if I have to but it's not preferable.


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InvisibleFriedEggS
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Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: nwalker248]
    #21733233 - 05/28/15 04:35 PM (8 years, 8 months ago)

Ohh I see.

Yea you could probably make it work with those swabs.  I'm sure 50 should be plenty. Give it a dry run first to practice it and rehearse what you'll be doing.


--------------------

(Yes, the egg is real)

How to post pics


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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: Using a bleach solution to sterilize area during inoculation and making a spore syringe? [Re: FriedEgg]
    #21733269 - 05/28/15 04:42 PM (8 years, 8 months ago)

Quote:

FriedEgg said:
Ohh I see.

Yea you could probably make it work with those swabs.  I'm sure 50 should be plenty. Give it a dry run first to practice it and rehearse what you'll be doing.




Yeah I've just done exactly that, just without the swabs for now as I'll be getting them tomorrow. Thanks for your help.


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