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Peaceful Soul
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Registered: 04/25/15
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Am I transferring my Agar correctly?
#21729114 - 05/27/15 03:13 PM (8 years, 8 months ago) |
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I must confess, I bought 2 grow kits 2 months ago. I took spores and made LC of all of them. I know, I Know....... But at that time I didn´t ;-)
I am now aware of the value of agar. Therefore I have started a projekt, trying to isolate some good genetics for my future grows. Working with what I got (LC)
I inoculated some LC in my Agar jars, let it grow for one week and then made my first transfers. I would like to know if I have chosen the right parts, or if I should have made my transfers from other places? Like maybe 6 o clock at the Mc Kenny pic?
Here is the pic´s:
Mc Kenny

AA+

I know, the AA+ pic might not be that useful. I guess that a lot less inoculated LC would have done a better job..... I´m still learning :-)
Edited by Peaceful Soul (05/27/15 03:37 PM)
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bodhisatta 
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Re: Am I transferring my Ager correctly? [Re: Peaceful Soul]
#21729171 - 05/27/15 03:31 PM (8 years, 8 months ago) |
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looks good on the first picture, doesn't really matter where you take from you don't know what you have until you fruit it anyway.
now just work on spelling your agar.
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Peaceful Soul
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Re: Am I transferring my Ager correctly? [Re: bodhisatta]
#21729189 - 05/27/15 03:40 PM (8 years, 8 months ago) |
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Thx, edited :-)
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FriedEgg



Registered: 09/22/14
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Re: Am I transferring my Agar correctly? [Re: Peaceful Soul]
#21729214 - 05/27/15 03:46 PM (8 years, 8 months ago) |
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in my opinion, the locations you chose are perfect. but i wouldn't cut so deep into the center. just grab the tip, the leading edge of mycelium.
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bodhisatta 
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Re: Am I transferring my Agar correctly? [Re: FriedEgg]
#21729244 - 05/27/15 03:58 PM (8 years, 8 months ago) |
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big chunks small chunks doesn't really matter at this point unless you're trying to find an isolate. and if you're trying to find an isolate just a reminder almost all of them will suck, so make a shitload you'll have to test all of them to find a keeper.
what do you do with your agar egg? got any examples.
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FriedEgg



Registered: 09/22/14
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Re: Am I transferring my Agar correctly? [Re: bodhisatta]
#21729306 - 05/27/15 04:12 PM (8 years, 8 months ago) |
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Yeah you're right... I've just been doing a lot of cloning lately and was more concerned about isolating away from any contams. Taking small transfers from the edge I figured was the best way to avoid any contamination. Right? Especially for someone like me who has almost no experience with identification. Better safe than sorry?
Now that I think about it, I only have pics of contaminated plates so I can go online and try to identify them In a few weeks I'll have about 10 different monocultures ready to test. I'll take pics when they're fully isolated. I know using all the strains in a clone usually works better but I just don't feel right about that. I'd rather just work with 1 and keep things simple and get consistent results each time.
Here's one I posted in the contam forum but it can't really be confirmed what it is. http://www.shroomery.org/forums/showflat.php/Number/21727029
 
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bodhisatta 
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Re: Am I transferring my Agar correctly? [Re: FriedEgg]
#21729345 - 05/27/15 04:22 PM (8 years, 8 months ago) |
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odd it almost looks like reishi mycelium. do you have any reishi you're working with at all?
to avoid contamination transfer as soon as the plate grows out enough to transfer and keep doing that. it's repeated quick transfers that leave behind bacteria and hopefully mold.
dryer than normal agar can help too especially if you're dealing with bacteria as it will make the colonizes smaller, and if you have a motile bacteria you're battling it will really help you out.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
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Re: Am I transferring my Agar correctly? [Re: bodhisatta]
#21729402 - 05/27/15 04:38 PM (8 years, 8 months ago) |
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Nope, no reishi. Just cubensis.
Dry? Do you mean hard? As in a higher agar to water ratio?
I've always wondered how to make my agar "drier". About 75% of my pasty plates get tons of condensation and then a few days later it looks like this. i'm not sure if it's the containers or if it's just really dirty clones. when i went back to pouring my own plates i don't see any condensation so oh well.

Sorry to threadjack but I guess it's somewhat on topic with OP's question
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Kizzle
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Re: Am I transferring my Agar correctly? [Re: FriedEgg]
#21729403 - 05/27/15 04:38 PM (8 years, 8 months ago) |
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Quote:
FriedEgg said: I know using all the strains in a clone usually works better but I just don't feel right about that. I'd rather just work with 1 and keep things simple and get consistent results each time.
They should be virtually identical.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
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Re: Am I transferring my Agar correctly? [Re: Kizzle]
#21729413 - 05/27/15 04:41 PM (8 years, 8 months ago) |
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What about that test pussyfart did with 10 PE isolates from 3 fruits? http://www.shroomery.org/forums/showflat.php/Number/18219379
He got wildly different results on all of them
and yea sorry again OP for threadjacking
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Kizzle
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Re: Am I transferring my Agar correctly? [Re: FriedEgg]
#21729447 - 05/27/15 04:50 PM (8 years, 8 months ago) |
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He doesn't say which ones came from the same mushroom
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Peaceful Soul
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Re: Am I transferring my Agar correctly? [Re: bodhisatta]
#21729486 - 05/27/15 04:59 PM (8 years, 8 months ago) |
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Quote:
bodhisatta said: if you're trying to find an isolate just a reminder almost all of them will suck, so make a shitload you'll have to test all of them to find a keeper.
bodhisattva: Please help me out here, because I feel a little lost! I have 8 quart jars of oat spawn, ready for inoculation. The plan is to use them for my first mono tub grow.
It was my plan to use agar to find an isolate to inoculate them with, but I do not want my grow to suck...... What to do? 
The pieces transferred from the pictures is now ready to the next transfer (T2) but I only have 4 1/2 pint jars left so I can not make a shitload of transfers. Is it an option to transfer one piece to each jar, and then take additionally pieces to inoculate the grain spawn? Will it still suck? Is it too isolated already?..... or would more isolating be beneficial?
I still have lots of the LC made of spores from my grow kit ( the LC that started the agar on the pictures) Is a MS grow really my best option? (and then wait a year or so with the isolate, as i can not se myself tripping all the time trying to get faster through all the isolates that I need to grown out)
My goal is to se my mono tub full of mushrooms that will be potent and come in flushes so I do not have to harvest 3 mushrooms every day for a month ha ha :-)
I am starting to see that this thread should properly have bin started in the noob forum, sorry about that :-)
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mushpunx
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Re: Am I transferring my Agar correctly? [Re: Kizzle]
#21729653 - 05/27/15 05:40 PM (8 years, 8 months ago) |
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Quote:
Kizzle said:
Quote:
FriedEgg said: I know using all the strains in a clone usually works better but I just don't feel right about that. I'd rather just work with 1 and keep things simple and get consistent results each time.
They should be virtually identical.
So if you isolate out each sector in a clone theyre going to be pretty much the same?
So if you wanna stablize a clone then you might as well take the best looking sector and fruit that one out to test it instead of going through and testing all the sectors like you would if you are isolating sectors from a culture grown from spore?
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FriedEgg



Registered: 09/22/14
Posts: 2,536
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Re: Am I transferring my Agar correctly? [Re: Peaceful Soul]
#21729974 - 05/27/15 07:18 PM (8 years, 8 months ago) |
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Quote:
Peaceful Soul said:
Quote:
bodhisatta said: if you're trying to find an isolate just a reminder almost all of them will suck, so make a shitload you'll have to test all of them to find a keeper.
bodhisattva: Please help me out here, because I feel a little lost! I have 8 quart jars of oat spawn, ready for inoculation. The plan is to use them for my first mono tub grow.
It was my plan to use agar to find an isolate to inoculate them with, but I do not want my grow to suck...... What to do? 
The pieces transferred from the pictures is now ready to the next transfer (T2) but I only have 4 1/2 pint jars left so I can not make a shitload of transfers. Is it an option to transfer one piece to each jar, and then take additionally pieces to inoculate the grain spawn? Will it still suck? Is it too isolated already?..... or would more isolating be beneficial?
I still have lots of the LC made of spores from my grow kit ( the LC that started the agar on the pictures) Is a MS grow really my best option? (and then wait a year or so with the isolate, as i can not se myself tripping all the time trying to get faster through all the isolates that I need to grown out)
My goal is to se my mono tub full of mushrooms that will be potent and come in flushes so I do not have to harvest 3 mushrooms every day for a month ha ha :-)
I am starting to see that this thread should properly have bin started in the noob forum, sorry about that :-)
That's actually a really easy question. The answer is: if the mycelium didn't come from cloned tissue, you want as many strains as possible. Don't worry, you have plenty of strains in those pics. The reason you don't want to isolate too much is because not all strains from spores are fruiting strains.
Your best and fastest strategy is to squirt spores (if theyre clean) into grain jars and grow it in a tub. I just did that 45 days ago and already have about 20 clones on agar growing out. I'm going to continue to isolate these clones until they are monocultures and then fruit them all to test various charictaristics.
In another month or two I'll have a really aggressive and potent strain that I can use for years and years. I'm only going to bioassay the most 3 or 4 aggressive ones because yea I don't really want to do 20 test trips either, lol. That's a lot of work and a lot of time.
And no this is not a noob topic. You're fine
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bodhisatta 
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Re: Am I transferring my Agar correctly? [Re: FriedEgg]
#21729980 - 05/27/15 07:21 PM (8 years, 8 months ago) |
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Most isolated will fruit just not well. You would have better luck just using spores besides it takes upwards of 10 transfers to get an isolate from spores most times so good luck you'll get started weeks from now with those jars. Just put spores to agar make one or two transfers and use it or grow and clone then use your clone culture
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mushpunx
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Re: Am I transferring my Agar correctly? [Re: bodhisatta]
#21730001 - 05/27/15 07:28 PM (8 years, 8 months ago) |
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bodhisatta said: Most isolated will fruit just not well. You would have better luck just using spores besides it takes upwards of 10 transfers to get an isolate from spores most times so good luck you'll get started weeks from now with those jars. Just put spores to agar make one or two transfers and use it or grow and clone then use your clone culture
I second this get some good clones to grow with and work on isolation in the mean time, so you can have some awesome grows while searching out those awesome cultures
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FriedEgg



Registered: 09/22/14
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Re: Am I transferring my Agar correctly? [Re: bodhisatta]
#21730019 - 05/27/15 07:34 PM (8 years, 8 months ago) |
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Yup.
Going to agar first is going to slow you down though. If you want the fastest way possible, do this:
Use an in vitro tek with about 1/2 inch of grass seed. Inoculate with a spore syringe at 4 different places. Be sure to space out the inoculation points. Don't case it. Fruit it as soon as its colonized. Clone the first few pins you see.
I don't think it can get much faster than that. You'll have a clone on agar in about 4 weeks.
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Edited by FriedEgg (05/27/15 08:23 PM)
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mushpunx
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Re: Am I transferring my Agar correctly? [Re: FriedEgg]
#21731217 - 05/28/15 04:42 AM (8 years, 8 months ago) |
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^ the problem with this is that spore syringes are very rarely clean enough to put to grain. Sure when I started cultivating I had sucess with it but the more work I did the more I realized this is not the best practice, especially if you have the means to work with agar.
It is much better to germinate your spores on agar and clean up the culture with a couple transfers before putting the culture to grains. IMO all grain spawn should start with a clean culture on agar. Besides, when I want a clone I dont want just any clone, I want a whole tub of pins to search for the fastest, most healthly looking gnarliest pin cluster so my clone will probably rock out, not just any old pin
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Edited by mushpunx (05/28/15 04:45 AM)
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Kizzle
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Re: Am I transferring my Agar correctly? [Re: mushpunx]
#21731921 - 05/28/15 09:59 AM (8 years, 8 months ago) |
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Quote:
mushpunx said:
Quote:
Kizzle said:
Quote:
FriedEgg said: I know using all the strains in a clone usually works better but I just don't feel right about that. I'd rather just work with 1 and keep things simple and get consistent results each time.
They should be virtually identical.
So if you isolate out each sector in a clone theyre going to be pretty much the same?
So if you wanna stablize a clone then you might as well take the best looking sector and fruit that one out to test it instead of going through and testing all the sectors like you would if you are isolating sectors from a culture grown from spore?
Yup.
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FriedEgg



Registered: 09/22/14
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Re: Am I transferring my Agar correctly? [Re: mushpunx]
#21732009 - 05/28/15 10:28 AM (8 years, 8 months ago) |
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Quote:
mushpunx said: ^ the problem with this is that spore syringes are very rarely clean enough to put to grain. Sure when I started cultivating I had sucess with it but the more work I did the more I realized this is not the best practice, especially if you have the means to work with agar.
It is much better to germinate your spores on agar and clean up the culture with a couple transfers before putting the culture to grains. IMO all grain spawn should start with a clean culture on agar. Besides, when I want a clone I dont want just any clone, I want a whole tub of pins to search for the fastest, most healthly looking gnarliest pin cluster so my clone will probably rock out, not just any old pin
You're 100% right. When I did this, my success rate was only 60% but I did about 15 jars so I still had options. But here I'm just talking about speed, not success rate or yield. Even better, do a few tubs at the same time.
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mushpunx
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Re: Am I transferring my Agar correctly? [Re: Kizzle]
#21732392 - 05/28/15 12:32 PM (8 years, 8 months ago) |
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Quote:
Kizzle said:
Quote:
mushpunx said:
Quote:
Kizzle said:
Quote:
FriedEgg said: I know using all the strains in a clone usually works better but I just don't feel right about that. I'd rather just work with 1 and keep things simple and get consistent results each time.
They should be virtually identical.
So if you isolate out each sector in a clone theyre going to be pretty much the same?
So if you wanna stablize a clone then you might as well take the best looking sector and fruit that one out to test it instead of going through and testing all the sectors like you would if you are isolating sectors from a culture grown from spore?
Yup.
Haha thx Kizzle. I made an actual thread about this today, but yea, think I got the answer now
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