Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Proving an LC
    #21726910 - 05/26/15 10:45 PM (8 years, 8 months ago)

So when can you call an lc clean?

If it colonizes cleanly? Do you have to fruit it? Cause a e I have an lc I tested on a few bags and they all colonized real purdy like but can I call it clean already or do I need to go to sub and try to fruit before I call it?


Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 21 hours
Trusted Cultivator
Re: Proving an LC [Re: Ghatti]
    #21726966 - 05/26/15 11:04 PM (8 years, 8 months ago)

Quote:

Ghatti said:
So when can you call an lc clean?



3-5 days after it's put on agar.....you should be able to tell by then....

Quote:

Ghatti said:
If it colonizes cleanly? Do you have to fruit it?



Yes, fruit/spawn it.

If it fruits without contamming, you have your answer.


Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Proving an LC [Re: PussyFart]
    #21726975 - 05/26/15 11:09 PM (8 years, 8 months ago)

I put it to agar the same time I put it to grain and it looked fine although was hard to tell since the drop rolled around like spore solution tends to making a mess of the plate.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Proving an LC [Re: Ghatti]
    #21727051 - 05/26/15 11:51 PM (8 years, 8 months ago)

Melt a divot in the agar with a red hot loop and place the drop in it. It will hold it amd prevent it from rolling around.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Proving an LC [Re: Pastywhyte]
    #21727563 - 05/27/15 06:39 AM (8 years, 8 months ago)

Put a drop on your loop and streak it across the plate in numerous zig zags. If the syringe is fucked where you first started streaking will have the most colonies of rouge bacteria and or mold.


Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Proving an LC [Re: bodhisatta]
    #21727663 - 05/27/15 07:37 AM (8 years, 8 months ago)

Good tips. Well if there was mold I didn't see it, or it looked very similar to mushroom myc and never sporelated.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Prevent Contamination lvleph 1,375 5 01/09/03 05:14 AM
by debianlinux
* Far into the LC Myc Matrix
( 1 2 3 all )
agar 9,458 44 05/26/05 01:36 PM
by Aeolus1369
* Cleaning perlite. ?? Golgi 11,048 14 11/02/16 11:21 AM
by mushboy
* myc isolation through LC HidingInPlainSight 549 4 05/23/05 09:41 AM
by IGnosticAbhorI
* what to add to incubator (TIT) water to prevent algae / stench ... ohmatic 1,887 4 04/26/05 10:21 AM
by ohmatic
* Protec Humidifier Cleaning Cartridge dobinky 418 2 12/06/04 07:45 PM
by intrigue
* Modifying Chronic for clean prints mickywilliams2 810 6 01/20/05 10:41 PM
by kiss
* Prevention of post first flush contamination. Baby_Hitler 1,357 3 11/01/02 09:49 AM
by theDEA

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
276 topic views. 15 members, 200 guests and 52 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.008 seconds on 14 queries.