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InvisibleHooray4Hoffman
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Registered: 11/19/12
Posts: 105
Spawning a stalled/slowed WBS jar? POLL!
    #21726382 - 05/26/15 08:21 PM (8 years, 8 months ago)

Let's say you have a 1 pint jar of WBS that slowed down until there was about a dime-sized spot visible against the glass that appeared uncolonized. It very slowly filled in, but not all the way, since rather than wait indefinitely you put it in the fridge.

You want to make a small tray to spawn this amount of grain to; you have prepped the sub and everything.

You also have a couple other pint jars of colonized WBS that you planned to hang on to for G2G.

Would you:
A. Use the first jar and risk that it has a contam and thus the tray will too
B. Use one of your good jars for the sake of the tray

If B, what would you do with the first jar -- something experimental but without too much effort, e.g. just open it up, maybe case the top and see if you can fruit right in the jar?
What would you do??
You may choose only one


Votes accepted from (05/26/15 06:17 PM) to (No end specified)
View the results of this poll



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InvisibleMad Season
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Registered: 09/16/12
Posts: 12,666
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Re: Spawning a stalled/slowed WBS jar? POLL! [Re: Hooray4Hoffman]
    #21726765 - 05/26/15 10:01 PM (8 years, 8 months ago)


Turned into:



--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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InvisibleHooray4Hoffman
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Registered: 11/19/12
Posts: 105
Re: Spawning a stalled/slowed WBS jar? POLL! [Re: Mad Season]
    #21727702 - 05/27/15 07:54 AM (8 years, 8 months ago)

^ Yeah I know that sometimes if grains are pressed up against the glass they will look normal i.e. not covered in white, but in this case it was one concentrated spot that just didn't seem to be in any hurry to fill in.

Not saying that's necessarily the explanation with yours, so good to see it turned out well.


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InvisibleMad Season
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Re: Spawning a stalled/slowed WBS jar? POLL! [Re: Hooray4Hoffman]
    #21727762 - 05/27/15 08:21 AM (8 years, 8 months ago)

That explanation isn't real. All wet uncolonized grains are bacterial. If they weren't it'd just be white. Grains stuck against the glass isn't what happens. That jar took a month to get that wet spot looking like how it did. I thought it stalled but it just verrrry slowly took it over

healthy jar with a few specks of bacteria.

Grains have bacterial endospores that can survive 2 hours of pressure cooking. If you see wet uncolonized spots, you need to up your pc time and work on your sterile techniques. If all you see in the stalled spot is wet grains, it's bacteria and the bulk substrate should have beneficial microbes to fight off bacteria.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleHooray4Hoffman
Researcher

Registered: 11/19/12
Posts: 105
Re: Spawning a stalled/slowed WBS jar? POLL! [Re: Mad Season]
    #21727970 - 05/27/15 09:39 AM (8 years, 8 months ago)

Interesting. Based on that, why don't we all PC for 2+ hrs rather than 90 mins? This is the first time I've ever seen a spot take so long, and this was 1 out of 12 jars.


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InvisibleMad Season
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Re: Spawning a stalled/slowed WBS jar? POLL! [Re: Hooray4Hoffman]
    #21728035 - 05/27/15 09:57 AM (8 years, 8 months ago)

That's what I'm thinking should happen. It should be a 2 hour minimum. Lots of members do 2 hours because 90 minutes just didn't cut it


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (05/27/15 10:02 AM)


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