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first time expert
practice makes perfect



Registered: 02/19/13
Posts: 1,246
Loc: top of mount washington
Last seen: 6 years, 4 months
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lc or agar for brf cakes
#21723798 - 05/26/15 07:14 AM (8 years, 8 months ago) |
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im sure this has been asked 1000 times( i have searched for a couple hrs) I have 8 lc's and about 15 clean plates. Im going to make a bunch of brf jars and was wondering if I should stick with agar which i know is clean, or should I knock them with the lc's? These are my first lc's so im not 100% sure what i can rule out as a dirty lc. they look good to me, clear/ white myc.
ive had good luck with agar but want to try the lc route. The only thing im worried about with the lc is getting the myc into my (used) syringe with out contams.
any input or tips would help... thanks
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
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Take your syringes apart and wrap the pieces all together in some foil or place them into a jar with a filtered lid and run it through your PC the next time you do grains/cakes and you will now have some sterile syringes.
You can test your LCs with your newly sterilized syringes by placing 1 drop or so on a fresh petri and checking to see what grows. How did you start your LC, from spores or agar? What was your recipe?
If you have clean myc on agar you can use your newly sterilized syringes, suck up some sterile water, and make a ghetto LI out of your petris. Just shoot some sterile water on your plate after flame sterilizing the needle, mix it around, and suck up the white goodness. (That's what she said)
Last year when I was still doing cakes I got away with using spore - LC - cakes and ended up getting quite a few decent harvests. The downside to that was I found out my LCs were past their prime when I started loosing a shit ton of cakes. LCs can work and work well, but you should be extra careful when using them.
Good luck on your grow, let us know how it goes
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Straya
Wot?!


Registered: 03/04/12
Posts: 705
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Re: lc or agar for brf cakes [Re: Dhearic]
#21723868 - 05/26/15 07:40 AM (8 years, 8 months ago) |
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Quote:
Dhearic said: Take your syringes apart and wrap the pieces all together in some foil or place them into a jar with a filtered lid and run it through your PC the next time you do grains/cakes and you will now have some sterile syringes.
You surely don't mean to PC the Liquid Cultures do you mate? That will kill them won't it?
Bro do you have some pics of your LC's? LC should be fine, if they look clean then go for it, just flame sterilise between each jar you inoculate and you shouldn't have a problem at all.
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 9 months, 3 days
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Re: lc or agar for brf cakes [Re: Straya]
#21723927 - 05/26/15 07:58 AM (8 years, 8 months ago) |
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Quote:
Straya said:
Quote:
Dhearic said: Take your syringes apart and wrap the pieces all together in some foil or place them into a jar with a filtered lid and run it through your PC the next time you do grains/cakes and you will now have some sterile syringes.
You surely don't mean to PC the Liquid Cultures do you mate? That will kill them won't it?
Bro do you have some pics of your LC's? LC should be fine, if they look clean then go for it, just flame sterilise between each jar you inoculate and you shouldn't have a problem at all.
If you PC a syringe full of LC it will definitely kill the myc as well as anything that was contaminating(if it has a contam) the LC. I'm also pretty sure if you PC a syringe full of anything it will be empty when you finish, I doubt that plunger will stay in the open position when there's 15psi pushing on it.

I used to wrap my empty, disassembled syringes in foil to run em through the PC but recently I've been dropping them into a jar with a filtered lid. I'll leave that jar as is until I need the syringes, at which time I'll open it in front of my hood and get to work.
You can get a decent idea of the cleanliness of an LC by looking at it but I still suggest putting a drop of it on agar. A million gajillion things you don't want can hide in an LC....
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
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Re: lc or agar for brf cakes [Re: Straya]
#21723932 - 05/26/15 08:01 AM (8 years, 8 months ago) |
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Quote:
Straya said: Bro do you have some pics of your LC's? LC should be fine, if they look clean then go for it
recipe for disaster! even after testing your LC's you may have ruined them.
I'm really curious as to how you get an agar wedge into a brf cake FTE? have you read this? http://www.shroomery.org/forums/showflat.php/Number/20429745/fpart/1/vc/1/nt/21
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unfortunategent
Stranger


Registered: 06/04/14
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Re: lc or agar for brf cakes [Re: spacechildo] 1
#21724013 - 05/26/15 08:36 AM (8 years, 8 months ago) |
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I use agar with brf all the time. Just don't use a dry filter layer, opting for tyvek or polyfil instead. Then u just drop the wedge on top of the cake and let colonization take place.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: lc or agar for brf cakes [Re: spacechildo]
#21724116 - 05/26/15 09:09 AM (8 years, 8 months ago) |
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Quote:
spacechildo said:
Quote:
Straya said: Bro do you have some pics of your LC's? LC should be fine, if they look clean then go for it
recipe for disaster! even after testing your LC's you may have ruined them.
I'm really curious as to how you get an agar wedge into a brf cake FTE? have you read this? http://www.shroomery.org/forums/showflat.php/Number/20429745/fpart/1/vc/1/nt/21
Turn it into a LI and have a dozen pf cakes fully colonized in under ten days.
Edited by insanemike (05/26/15 09:12 AM)
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: lc or agar for brf cakes [Re: insanemike]
#21724137 - 05/26/15 09:15 AM (8 years, 8 months ago) |
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Quote:
insanemike said:
Quote:
spacechildo said:
Quote:
Straya said: Bro do you have some pics of your LC's? LC should be fine, if they look clean then go for it
recipe for disaster! even after testing your LC's you may have ruined them.
I'm really curious as to how you get an agar wedge into a brf cake FTE? have you read this? http://www.shroomery.org/forums/showflat.php/Number/20429745/fpart/1/vc/1/nt/21
Turn it into a LI and have a dozen pf cakes fully colonized in under ten days.
that was my idea too. hence the link. I've seen a couple of those who put agar on top of the brf cake and use grain jar lids but I've never seen those colonize fast.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: lc or agar for brf cakes [Re: spacechildo]
#21724355 - 05/26/15 10:35 AM (8 years, 8 months ago) |
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I recently knocked up a bunch of PF jars by placing a wedge on top and using grain filter.
They colonized at a very even rate but yeah, quite slow.
Not only wicked slow but also quite hard to get dialed in right, cakes are too fuasy. I find small trays or mini mono much better for tests Recently I saw the sugguestion to make up some half pint jars with some agar in the bottom and then inoculate with a wedge of your culture. When its colonized you can add sterile water, shake it up and suck up the mycelium laden water, kind of like a liquid innoculant.
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Edited by mushpunx (05/26/15 10:39 AM)
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first time expert
practice makes perfect



Registered: 02/19/13
Posts: 1,246
Loc: top of mount washington
Last seen: 6 years, 4 months
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Re: lc or agar for brf cakes [Re: Dhearic]
#21724730 - 05/26/15 12:39 PM (8 years, 8 months ago) |
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i would be using the agar as a li with sterile water. Quote:
Dhearic said: Take your syringes apart and wrap the pieces all together in some foil or place them into a jar with a filtered lid and run it through your PC the next time you do grains/cakes and you will now have some sterile syringes.
Y
thats a really good idea!! I have about 6 used syringes sitting around, i always soak them in iso,dry and suk up boiling water, pain in the ass to say the least. im still curious about the lc's. I made them with maple syrup, then droped a chunk of agar in them. only one lc jar has a thick solid white mass floating up top. all the others have see-through myc towards the bottom of the lc's.
I guess at this point, it would be smartest to use agar li, and one cake for each lc jar.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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I think you should make a few agar dishes and either do LI or cut wedges of clean agar and put it into a few LCs
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 9 months, 3 days
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Re: lc or agar for brf cakes [Re: Mad Season]
#21725622 - 05/26/15 05:13 PM (8 years, 8 months ago) |
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Do at least 2-3 cakes or 1 petri dish(multiple innoc points) per LC to get a good feel for how clean they are, if you only do one per LC you won't know whether it was you that fucked up that one innoc or if the LC was bad. Also you need to use expert sterile technique because you may introduce contams anytime you pull from your LC. It's a higher risk method imo. 3 of 3 bad means either you suck or the LC is dirty 
I'm looking to build my muda LI and tribal container setup in the next few weeks after a payday or 2. At the moment I've got some grain jars cooking with a pint 2/3 full of water and a quart holding some spoons and syringes.
I plan on dropping some wedges into a few of the jars and for my prettiest plate I'm going to drop it into the sterilized water and mix the shit out of it with the spoons/forks/whatever that I've got cooking then suck it up for a quick and dirty LI.
On a side note I picked up 50lbs of cleaned oats for 12 bucks today, epic fucking win
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: lc or agar for brf cakes [Re: spacechildo]
#21726588 - 05/26/15 09:13 PM (8 years, 8 months ago) |
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Quote:
spacechildo said:
Quote:
insanemike said:
Quote:
spacechildo said:
Quote:
Straya said: Bro do you have some pics of your LC's? LC should be fine, if they look clean then go for it
recipe for disaster! even after testing your LC's you may have ruined them.
I'm really curious as to how you get an agar wedge into a brf cake FTE? have you read this? http://www.shroomery.org/forums/showflat.php/Number/20429745/fpart/1/vc/1/nt/21
Turn it into a LI and have a dozen pf cakes fully colonized in under ten days.
that was my idea too. hence the link. I've seen a couple of those who put agar on top of the brf cake and use grain jar lids but I've never seen those colonize fast.
I didn't click the link until after I posted. For me, since going the route of LI, it has made LC's obsolete.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: lc or agar for brf cakes [Re: insanemike]
#21727315 - 05/27/15 03:13 AM (8 years, 8 months ago) |
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once you learn how stuff works and whats really going on in your liquids or on your grains/agar whatever you realize why LI is such a safer bet than the nutrient LC's!
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: lc or agar for brf cakes [Re: spacechildo]
#21727353 - 05/27/15 03:39 AM (8 years, 8 months ago) |
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Well yeah. In order to start clean, you must start on agar. Why wait the extra 7-10 days after that colonizing a LC when LI is ready to go same day the agar plate is.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: lc or agar for brf cakes [Re: insanemike]
#21727399 - 05/27/15 04:16 AM (8 years, 8 months ago) |
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yeah that too, but I got time. I was more thinking about whats going on in a LC even before you inoc it. Have you seen all of bodhisattas latest main threads? explains it pretty damn well!
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: lc or agar for brf cakes [Re: spacechildo]
#21727409 - 05/27/15 04:24 AM (8 years, 8 months ago) |
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Nah but I will check them out. Thanks, love when I have something good to read.
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first time expert
practice makes perfect



Registered: 02/19/13
Posts: 1,246
Loc: top of mount washington
Last seen: 6 years, 4 months
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Re: lc or agar for brf cakes [Re: insanemike]
#21728340 - 05/27/15 11:22 AM (8 years, 8 months ago) |
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thanks for the replys!! Li deff is just as easy as lc. the risk of contams with the lc is what has me wondering. Im trying to get the fastest results without trich or yeast.
when i made the lc's, i didnt put any glass or marbles in the jars so im not sure how im going to mix them up, maybe sterlize a fork or somthing stainless so i can break up the mats of myc.
I have a room designed for my mycology work, plastic sheets hung with all the seams taped and i spray bleach/antibacterial dawn soapy water on the entire surface of the walls, ceiling and floor before I start working in my sab.
Li has worked for me before so maybe stick with that for the most of my grow, I guess I can use the lc's as an experiment/learning crash n burn failure, might end up with some clean cakes in the process???
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insanemike

Registered: 02/23/14
Posts: 4,272
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: lc or agar for brf cakes [Re: insanemike]
#21728693 - 05/27/15 01:12 PM (8 years, 8 months ago) |
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Wow man I wish I had the ambition to bleach down my room like that before I start work
Isnt that a little overkill though man? I kinda think all that matters is your clothes/body and your still air box
But like I said I wish I had the drive to wash my room floor to ceiling every time I work Im sure it helps
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 Amateur Mycologists United AMU Q&A
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