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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 470
Last seen: 2 days, 9 hours
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is this contaminated? 3rd generation from agar
#21723348 - 05/26/15 01:37 AM (8 years, 8 months ago) |
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Ok so I did agar to agar 3 times got what I thought was a clean mono agar dish and took a lil bit to seed. it was at 100% a week ago but I wanted to make sure it was fully colonized so I waited one more week to go to bulk but I'm now that I'm ready to go to bulk it's getting kinda fluffy on the top idk if this is just a fully colonized seed striving to find new seed or if this is some sort of contam like mildew? These are the best photos I could get with my phone and its all white in real life any color difference you see in picture is just the camera being stupid. Also when making these seeds I also made another agar with this same stuff and it's contam free
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: is this contaminated? 3rd generation from agar [Re: more4u2c]
#21723352 - 05/26/15 01:39 AM (8 years, 8 months ago) |
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It looks contaminated to me. I'd toss it
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DensePlacebo
Stranger



Registered: 05/14/15
Posts: 186
Loc: canada
Last seen: 6 years, 4 months
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Re: is this contaminated? 3rd generation from agar [Re: Mad Season]
#21723397 - 05/26/15 02:13 AM (8 years, 8 months ago) |
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It looks fine to me. My wbs does that on the top in the space between the lid and the wbs. But I've only done two batches,24 quart jars. If its 100% white I'd say its good.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: is this contaminated? 3rd generation from agar [Re: DensePlacebo]
#21724107 - 05/26/15 09:07 AM (8 years, 8 months ago) |
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First pic on the top isn't mycelium. That is for certain. Idk what but definitely not good. That 1 jar would ruin a tub so it isn't worth it. Just toss and use clean spawn.
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MikeBearPig
Not liked.

Registered: 07/15/14
Posts: 2,319
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Re: is this contaminated? 3rd generation from agar [Re: Mad Season]
#21724584 - 05/26/15 11:59 AM (8 years, 8 months ago) |
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Quote:
Mad Season said: First pic on the top isn't mycelium. That is for certain. Idk what but definitely not good. That 1 jar would ruin a tub so it isn't worth it. Just toss and use clean spawn.
Looks like tom growth on top to me. Looks good, I don't see anything wrong.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: is this contaminated? 3rd generation from agar [Re: MikeBearPig]
#21724673 - 05/26/15 12:21 PM (8 years, 8 months ago) |
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It's definitely off. Ain't no tomentose I've ever seen. Definitely darker color than the rest of the jar. If it was tomentose it'd still be white.
Sticks out like a sore thumb to me..
Edited by Mad Season (05/26/15 12:22 PM)
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DensePlacebo
Stranger



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Posts: 186
Loc: canada
Last seen: 6 years, 4 months
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Re: is this contaminated? 3rd generation from agar [Re: Mad Season]
#21725018 - 05/26/15 02:10 PM (8 years, 8 months ago) |
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He said it was all white. Any color difference is the camera. With that many clean agar plates what are the odds its contam. If its all white I think its fine. Spawn just that one jar to a mini tub or something.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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MikeBearPig
Not liked.

Registered: 07/15/14
Posts: 2,319
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Re: is this contaminated? 3rd generation from agar [Re: DensePlacebo]
#21725030 - 05/26/15 02:13 PM (8 years, 8 months ago) |
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Quote:
DensePlacebo said: He said it was all white. Any color difference is the camera. With that many clean agar plates what are the odds its contam. If its all white I think its fine. Spawn just that one jar to a mini tub or something.
2nd this. I have had jars with many shades of white. It happens sometimes. Almost all contams won't be white anyways, at least the ones you will notice in the jar.
If you have a white spawn and it made it to full colonization, its most likely clean, not full proof though. Since you said it was on agar, i REALLLLLY don't think any white fluffy contam hitched a ride.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: is this contaminated? 3rd generation from agar [Re: MikeBearPig]
#21725216 - 05/26/15 03:09 PM (8 years, 8 months ago) |
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Yeah if you really wanted to play around with it I'd just spawn to a mini mono or a tray.
I don't really know how camera angles could make the entire surface look a completely different color. I know mycelium is different colors but it doesn't look THAT off... When I take a pic of white mycelium it's white mycelium. Different angles don't change it.
I've had stuff well over 3 clean transfers that contaminated. I get a contaminated jar every so often and all I use is clean agar. Hell even see agar contaminations after lots of clean transfers. Every millimeter on us has well over 200000 spores so it's not really that unheard of to have a contamination during inoculation. It's not that unheard of to have 1 dish be bad and 1 dish be good. That's why we clone to multiple dishes in case we see contaminations in one and have a back up that didn't contaminate. I always transfer to 2 dishes now in case it does contaminate.
Edited by Mad Season (05/26/15 03:14 PM)
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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 470
Last seen: 2 days, 9 hours
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Re: is this contaminated? 3rd generation from agar [Re: MikeBearPig]
#21725241 - 05/26/15 03:16 PM (8 years, 8 months ago) |
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Thanks guys for your input here is a sorta better pic of them with some daylight to get a better look at the all white jars
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: is this contaminated? 3rd generation from agar [Re: more4u2c]
#21725255 - 05/26/15 03:20 PM (8 years, 8 months ago) |
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Lol well those ones look fine. If your jar looks like those then just use it. I've never seen a camera make it look that off before lol.
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MikeBearPig
Not liked.

Registered: 07/15/14
Posts: 2,319
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Re: is this contaminated? 3rd generation from agar [Re: more4u2c]
#21725256 - 05/26/15 03:20 PM (8 years, 8 months ago) |
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Quote:
more4u2c said: Thanks guys for your input here is a sorta better pic of them with some daylight to get a better look at the all white jars

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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 470
Last seen: 2 days, 9 hours
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Re: is this contaminated? 3rd generation from agar [Re: MikeBearPig]
#21725488 - 05/26/15 04:32 PM (8 years, 8 months ago) |
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Lol woohoo I was worried the fluff was a problem lol they are all white damn camera phone in the dark dim room makes horrible pictures lol
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