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Offlinenoobs1988
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how to make the most out of my 2 spore syringes and quick harvest
    #21721453 - 05/25/15 03:44 PM (8 years, 8 months ago)

Hello shroomery. It's been a while since I've been here. Had my very 1st 1000w coco grow going on that consumed my interest. Then I got robbed 75% of it... I broke the golden rule, DONT TELL NO ONE.  Some people have to learn the hard way but since then, I have definitely considered my lesson learned.  Anyways, now I'm gearing up do a bulk mushroom grow.

I have 2 spore syringes of B+.  I've done abt 6 grows, last 4 were monotubs which I loved. So I want to stay mono. 

I usually do multispore to grain to hpoo/coir. I will do that, but also want to experiment with hay and I have gypsum and crushed ostyster shell though I've never used any of that stuff. Just grain coco coir and hpoo.

Well I have 20 cc's to work with. My pressure cooker hold 7 quarts so what I was thinking was I could do 6 quarts and 1 liquid culture jar per run and use 10cc's for that. Then the next day do that again.  So I'll have 12 grain jars and 2 liquid culture jars. Only difference is I will do one LC in Karo and the other in honey.

Then I'm going to order prepoured agar disk and use LC as inoculate. I know its cheaper to make agar your self but I have no experience with any of that and I need to give myself the best chance of success.

Any tips on what kind of agar I should get. I saw different kinds. Any links would be awesome.

Well think that sound OK the start with?


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: how to make the most out of my 2 spore syringes and quick harvest [Re: noobs1988]
    #21721586 - 05/25/15 04:13 PM (8 years, 8 months ago)

Or you could just put 1 or 2 drops of MS solution on each plate, then it'll last forever:shrug:
There's 20 drops for every cc.

20cc x 20 drops = 400 plates knocked up with 1 drop

Don't buy premade agar, just buy some agar and do it yourself and save lots of money and time.


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Offlinenoobs1988
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: Buck513]
    #21721678 - 05/25/15 04:40 PM (8 years, 8 months ago)

Yes. But my problem is I don't have agar on hand and I figure by the time I order, my jars and lc would already be on its way to complete.

What's a good cheap supplier of agar. What kind should I get. Anything else I should get like dextrose or anything.


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OfflineJsneeb
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: noobs1988]
    #21722246 - 05/25/15 07:36 PM (8 years, 8 months ago)

I was about to pull the trigger on these Agar plates. But then went the home made approach.

They looked good and the price is OK since you car really do alot with 10 plates if you're efficient and don't fuck up on sterile tek.

http://www.amazon.com/EZ-BioResearch-Pre-poured-Plates-plates/dp/B004MKHNJK/ref=sr_1_1?ie=UTF8&qid=1432604104&sr=8-1&keywords=pre+poured+agar+plates


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: how to make the most out of my 2 spore syringes and quick harvest [Re: Jsneeb]
    #21722268 - 05/25/15 07:43 PM (8 years, 8 months ago)

Seriously? 20+ dollars?

I can buy 20 grams of agar for about $1.50-2.00
I can buy six 8-count packs of glad mini rounds for less than $25.



I would rather spend 2 or 3 extra dollars and get 60+ plates LOL


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Offlinenoobs1988
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: Buck513]
    #21722615 - 05/25/15 09:04 PM (8 years, 8 months ago)

Thanks for the link. Yeah I'll probably buy those 10pk. That'll be plenty to get me started.


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: how to make the most out of my 2 spore syringes and quick harvest [Re: noobs1988]
    #21722646 - 05/25/15 09:11 PM (8 years, 8 months ago)

:blowmybrainsout:


--------------------
Fail to plan and you plan to fail.

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Offlinekn33b
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: Buck513]
    #21722732 - 05/25/15 09:29 PM (8 years, 8 months ago)

Quote:

Buck513 said:
:blowmybrainsout:



He did say he needs to learn the hard way...


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InvisibleDoryBlazed
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: kn33b]
    #21722755 - 05/25/15 09:33 PM (8 years, 8 months ago)

You know from one of our sponsors out-grow.com, you can get premixed dehydrated PDA or MEA for $15. That is enough to make 120 plates.


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Offlinenoobs1988
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: DoryBlazed]
    #21723023 - 05/25/15 10:58 PM (8 years, 8 months ago)

But then I nedd plates and have to prepare  them. I also said I want to start with the vest chance of success and I think buying pre poured agar would be safer than me making it for this one time.

I already said I k ow its cheaper to make it yourself. Stop trying to chop my head off.


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InvisibleNumeroEno
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: noobs1988]
    #21723065 - 05/25/15 11:13 PM (8 years, 8 months ago)

Seriously, it's pretty easy. Go to your closest whole foods and buy agar powder, dextrose powder, and potato starch. I guarantee they will have all three.

I get my petri dishes from http://www.cynmar.com -- they have a 35 dollar minimum order but you get a shitload of petris for 35 bucks.

Also if there is a brewing supply store in your area you can get a pound of malt extract for like 5 bucks.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
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DOG FOOD AGAR

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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: how to make the most out of my 2 spore syringes and quick harvest [Re: NumeroEno]
    #21723069 - 05/25/15 11:14 PM (8 years, 8 months ago)



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OfflinePinPornProducer
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: NumeroEno]
    #21723091 - 05/25/15 11:20 PM (8 years, 8 months ago)

1/2 pint jars work great also, I use em because I have 72 of them from when I did cakes, may as well put em to good use of ya got em


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InvisibleNumeroEno
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: insanemike]
    #21723092 - 05/25/15 11:20 PM (8 years, 8 months ago)

That too.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineRosen_Rot
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: PinPornProducer]
    #21723291 - 05/26/15 12:58 AM (8 years, 8 months ago)

Nice rack! I need to make one of those as I always find trouble to store my jars and I keep running out of cardboard boxes haha

I would do what other posters have suggested. Buy from a sponsor, get agar ingredients and buy a ton of petris. Inoculate all and you'll be going forever

If you are in a hurry, find your local lab supplies store and get some petris and then go a whole food store and ask for agar. Trust me, if I can find agar in my area than there is agar everywhere else

I also wanted to say thanks for the links. I still need to extend my knowledge on that one :super:


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Offlinenoobs1988
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Re: how to make the most out of my 2 spore syringes and quick harvest [Re: insanemike]
    #21723459 - 05/26/15 02:55 AM (8 years, 8 months ago)

Quote:

insanemike said:
http://www.shroomery.org/forums/showflat.php/Number/19208976




Just read this thread. That looks super freaking easy. The one thing I've never seen is agar agar. Or agar powder. And I worked at Walmart 3 years. Where do they normally stock that stuff?

Also, I went to Walmart today to look at the jars.  It's close go harvest season and they got all kinds of canning stuff. I saw these tiny jars that should work great.

Also about about deep is a good depth to pour?


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: how to make the most out of my 2 spore syringes and quick harvest [Re: noobs1988]
    #21723470 - 05/26/15 03:09 AM (8 years, 8 months ago)

I buy my agar from out-grow.com. buy the mea agar premix, that's what I started off with. The glad mini rounds have the most bang for your buck. I use 15ml per mini round.


Edited by insanemike (05/26/15 03:10 AM)


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